Antibiogram of E. coli serotypes isolated from children aged under five with acute diarrhea in Bahir Dar town

Background: Diarrheal disease and its complications remain a major cause of morbidity and mortality in children. The prevalence and antibiogram of E. coli as causative agents of diarrhea vary from region to region, and even within countries in the same geographical area.Objectives: To determine the serotype and antimicrobial susceptibility of E. coli in children under-five years of age.Methods: A cross-sectional study was conducted among 422 children with diarrhea from December 2011 to February 2012. Identification of E. coli and antimicrobial susceptibility testing were done following standard procedures.Results: The overall isolation rate of E. coli was 48.3%. Poly 2 sero-groups, poly 3 sero-groups, poly 4 sero-groups and E. coli O157:H7 accounted for 80 (39.2%), 40 (19.6%), 25 (12.3%), and 59 (28.9%) of the isolates, respectively. Poly 2 sero- groups, constituting isolates belonging to enteropathogenic E. coli were the most commonly isolated serotypes. E. coli exhibited high levels of antimicrobial resistance to ampicillin (86.8%), tetracycline (76%) and cotrimoxazole (76%). Low levels of resistance to ciprofloxacin (6.9%) and norfloxacin (9.3%) were documented.Conclusion: High prevalence of diarrheagenic E. coli compounded by alarming antimicrobial resistances is a serious public health problem. Regular determination of antibiogram and public education are recommended.Keywords: E. coli, antimicrobial susceptibility, diarrhea, Ethiopi

Outbreak of Aeromonas hydrophila associated with the parasitic infection Ichthyophthirius multifiliis in pond of African catfish (Clarias gariepinus) fingerlings at Sebeta, Ethiopia

Outbreak of a disease was observed on African catfish (Clarias gariepinus) fingerlings manifested by white nodules all over the body and hemorrhage in the skin that occurred on June 20, 2011 in an earthen pond at Sebeta, Ethiopia. The outbreak was investigated by using a combination of methods that included clinical observations, gross and histopathology examination and bacterial isolation. On histopathological  examination co-infection of Aeromonas hydrophila with Ichthyophthirius multifiliis a holotrichous ciliate, was found to be the cause of the outbreak. In order to control the outbreak, the fish density was reduced and the fish were removed and treated with sodium chloride (3%) and moved to another properly disinfected pond that contains fresh and good quality water. The former pond was drained and left empty for two  weeks to dry and then lime was added over it before filling it with water. The sick fish got cured after three weeks and no new case was observed; which may be due to development of immunity or the intervention measures taken to control the problem. This intervention protocols need to be further investigated in a properly designed experiment as a possible control of co-infection of these two pathogens in catfish  fingerlings.Keywords: Aeromonas hydrophila, Co-infection, Ethiopia, Ichthyophthirius multifilii

Farm diversification in the central highlands of Ethiopia: Patterns determinants and its effect on household income

Crop-livestock production is the major farming system in the highlands of Ethiopia. This study aimed to describe crop-livestock diversification pattern, examine determinants of diversification patterns, and evaluate effects of diversification on household income. Principal component analysis (PCA), seemingly unrelated regression (SUR) and ordinary least square (OLS) regression models were employed. Five major crop-livestock diversification patterns: sheep and goat, staple crops, chicken, vegetables, and animal feed-based farming were identified. The SUR model revealed that sex, education, income, extension contact, land size, market and road distance, irrigated land, and household size were significant factors that influence crop-livestock diversification patterns. It is also found that sheep and goat, vegetable, and chicken-based farming were significant production patterns that had positive effects on household income. We suggest that adoptive and adaptive agricultural practices such as small-scale irrigation, chicken rearing and sheep-based production patterns are the most potential farming systems in the highlands of Ethiopia

Maternal dietary diversity and micronutrient adequacy during pregnancy and related factors in East Gojjam Zone, Northwest Ethiopia, 2016.

BackgroundMonotonous and less diversified diets are associated with micronutrient deficiency. Evidence on maternal dietary intakes during pregnancy is essential to achieve the 2025 global nutrition target and reduce maternal and child mortalities. This study assessed pregnant women's dietary diversity and identified factors associated with inadequate dietary diversity in East Gojjam Zone.MethodsWe conducted a community-based cross-sectional study between April and June 2016. Eight hundred thirty-four pregnant women were randomly sampled. The Women Dietary Diversity Score tool developed by the Food and Agricultural Organization (FAO) and Food and Nutrition Technical Assistance (FANTA) was used. Data were entered into EpiData with double entry verification, and analysis was done using IBM SPSS version 20. Level of significance was set to P ResultsThe mean (±SD) dietary diversity score was 3.68 (±2.10). Inadequate dietary diversity was prevalent in 55% [95% CI (52.3-59.3%)] of pregnant women, or indirectly micronutrient was inadequate in more than half of the pregnant women. Commonly consumed dietary groups were legumes, nuts, and seeds (85.5%) followed by starchy staples (64.7%). Inadequate dietary diversity was higher among non-educated [Adjusted Odds Ratio (AOR) = 7.30, 95% CI (2.35-22.68)] compared to college and above completed women. Wealth index had significant association with dietary diversity, in which women in the poorest [AOR = 8.83, 95% CI, (1.60-48.61)], poorer [AOR = 6.34, 95% CI (1.16-34.65)], poor [AOR = 8.46, 95% CI (1.56-45.70)], and richer [AOR = 6.57, 95% CI (2.16-20.01)] had higher odds of inadequate dietary diversity. Those who had not received dietary counseling had three folds [AOR = 3.31, 95% CI (1.49-7.35)] of inadequate dietary diversity compared to their counterparts. Less likelihood of inadequate dietary diversity was among women with an increased meal frequency [AOR = 0.53, 95% CI (0.38-0.74)].ConclusionConsumption of less diversified food during pregnancy is common in the study area. Adequacy of micronutrients is insufficient for more than half of the studied pregnant women. We conclude that being non-educated affects pregnant women to depend on less diversified diet. Providing dietary counseling during pregnancy can improve nutritional practice for pregnant women

Evaluation of bio-physiological and yield responses of stay green QTL introgression sorghum lines to post-flowering drought stress

Drought is the most drastic abiotic stress that limits crop production in sub-Sahara Africa. Sorghum ( Sorghum bicolor (L.) Moench) is among the most climate resilient cereals of the future due to its tolerance to drought and heat. Thus, identification of tolerant genotypes and plant characteristics that contribute to drought adaptation is timely. The objective of this study was to evaluate the response of 12 genotypes, including seven Stay-green (Stg) QTL introgression sorghum lines and their two donor; and three recurrent parents, to induced post-flowering drought stress. The recurrent parents were varieties released for drought prone lowlands of the country, and the donor parents were lines known to have stay-green properties. The study was conducted at Werer, in the Great Rift-valley of Ethiopia, during the off-season of 2014 under well-watered and water limited conditions. Significant differences (P<0.05) were observed among genotypes under water deficit regimes, for all measured physiological, biochemical (proline content), yield and yield related traits. Three Stg QTL introgression lines showed better tolerance to drought than their recurrent parents, as reflected by yield and maintenance of green leaf area. Three Stg QTL introgression lines, Teshale/E36-1, Gambella/B35-5 and Meko/B35-12, gave the highest grain yield under post-flowering drought stress, and B35 and Teshale/B35 gave the poorest yield under similar conditions. Therefore, these improved lines bear potential for use as alternatives in breeding programmes and for possible release in parts of the country where post-flowering drought stress is frequent. Proline content, weight of hundred seeds (HSW) and CO2 assimilation are effective screening tools for Stg property due to their high correlation with other parameters.La s\ue8cheresse est le stress abiotique le plus drastique qui limite la production agricole en Afrique Sub-saharienne. Le sorgho ( Sorghum bicolor (L.) Moench) est parmi les c\ue9r\ue9ales les plus r\ue9silientes au climat pour le futur d\ufb \ue0 sa tol\ue9rance \ue0 la s\ue9cheresse et \ue0 la chaleur. Donc, l\u2019identification des g\ue9notypes tol\ue9rants et les caract\ue9ristiques des plants qui contribuent \ue0 l\u2019adaptation \ue0 la s\ue9cheresse est opportune. L\u2019objectif de cette \ue9tude \ue9tait d\u2019\ue9valuer la r\ue9ponse de 12 g\ue9notypes, comportant sept lign\ue9es de sorgho conf\ue9rant l\u2019introgression des QTL des attributs verts (Stg), leurs deux donneurs\ua0; et les trois parents r\ue9currents, au stress de la s\ue9cheresse post floraison induite. Les parents r\ue9currents ont \ue9t\ue9 les vari\ue9t\ue9s lib\ue9r\ue9es sensibles \ue0 la s\ue9cheresse dans les plaines du pays, et les donneurs ont \ue9t\ue9 les lign\ue9es connues pour leurs propri\ue9t\ue9s relatives aux attributs verts. L\u2019\ue9tude a \ue9t\ue9 conduite \ue0 Werer, dans la grande vall\ue9e du Rift d\u2019Ethiopie, pendant la contre saison de 2014 dans des conditions d\u2019irrigation abondante et d\u2019irrigation limit\ue9e. De diff\ue9rences significatives (P<0.05) ont \ue9t\ue9 observ\ue9es entre les g\ue9notypes sous des r\ue9gimes de d\ue9ficit hydriques, pour tous les traits physiologiques, biochimiques (teneur en proline), le rendement et les composantes de rendement. Trois lign\ue9es d\u2019introgression de Stg QTL ont montr\ue9 de meilleure tol\ue9rance \ue0 la s\ue9cheresse que leurs parents r\ue9currents, comme refl\ue9t\ue9 par le rendement et la maintenance de la surface verte de la feuille. Trois lign\ue9es d\u2019introgression de Stg QTL, Teshale/E36-1, Gambella/B35-5 et Meko/B35-12, ont donn\ue9 le plus haut rendement sous le stress de s\ue9cheresse de post floraison, et B35 et Teshale/B35 ont donn\ue9 le plus faible rendement dans des conditions similaires. En cons\ue9quence, ces lign\ue9es am\ue9lior\ue9es d\ue9tiennent tout le potentiel pour leur usage comme alternatives dans des programmes d\u2019am\ue9lioration g\ue9n\ue9tiques et pour une lib\ue9ration possible dans les r\ue9gions du pays o\uf9 le stress de la s\ue9cheresse post floraison est fr\ue9quent. La teneur en proline, le poids de 100 graines (HSW) et l\u2019assimilation du CO2 sont des outils effectifs de criblage pour la propri\ue9t\ue9 de Stg due \ue0 leur forte corr\ue9lation avec les autres param\ue8tres

Study of growth and physiological characters in stay-green QTL introgression Sorghum bicolor (L.) lines under post-flowering drought stress

This experiment was carried out to evaluate the growth, physiological and yield traits of stay-green (Stg) QTL introgression sorghum lines, which were developed by the Ethiopian Institute of Agricultural Research in collaboration with ICRISAT between 2006 and 2008, under induced post-flowering drought stress. It involved a total of 12 genotypes including seven Stg QTL introgression lines, two Stg donor parents and three senescent recurrent parents. It was organized in a split plot design with three replications under well-watered and induced drought stress growing conditions at Melka Werer, Ethiopia during the post-rainy season of 2014. Analysis of variance revealed that the effect of moisture regimes on all measured traits was significant (P > 0.05). Differences among the genotypes and genotypeby- water regime interaction were also significant (P > 0.05) for all the traits considered. Postflowering drought stress was observed to significantly reduce most of the growth, physiological and yield related traits. The Stg introgression lines Meko/B35-selection 120, Teshale/B35-selection 2 and Teshale/E36-1 showed better drought stress tolerance properties than the rest of the genotypes based on the measured growth and physiological traits. These introgression lines also showed better grain yield than their recurrent parents under post-flowering drought stress and can be used as new versions of the existing varieties (served as recurrent parents) and for future breeding programs. Furthermore, leaf chlorophyll content, assimilation rate, transpiration rate, water use efficiency, root length and root dry weight were found to have strong correlation and can be used to screen genotypes for post-flowering drought tolerance

Does certified organic agriculture increase agroecosystem health? Evidence from four farming systems in Uganda

Over the past decades, the number of certified organic farms have increased significantly in Uganda. One assumption is that certified organic agriculture contributes to economic, social and ecological health of agroecosystems. In the literature, however, there is thin empirical evidence to support such claims. We therefore developed health indicators and contrasted data from four Ugandan farming systems with principles and objectives of organic agriculture. We identified four health patterns (ecology-driven, economically struggling, socially-driven, and hanging in) demonstrating the impact of farm management on agroecosystem health and trade-offs between health domains. Ecological farm health is strengthened only if the conversion goes beyond ‘organic by default’. Market-oriented specialization can create lock-in situations if production strategies cannot be changed easily. Food shortages occur when additional income from certified production does not compensate for the reduced area and effort devoted to food crops. We conclude that the positive effects of organic certification on agroecosystem health cannot be taken for granted. Interventions promoting organic agriculture should acknowledge risks smallholder farmers take by converting to cash crop-oriented certified organic farming. A challenging question will be how aspects of wellbeing and social health can be translated into certification standards and thus product attributes

Knowledge and perception towards net care and repair practice in Ethiopia.

BACKGROUND: Long-lasting insecticidal nets (LLINs) are a key malaria control intervention. Although LLINs are presumed to be effective for 3 years under field or programmatic conditions, net care and repair approaches by users influence the physical and chemical durability. Understanding how knowledge, perception and practices influence net care and repair practices could guide the development of targeted behavioural change communication interventions related to net care and repair in Ethiopia and elsewhere. METHODS: This population-based, household survey was conducted in four regions of Ethiopia [Amhara, Oromia, Tigray, Southern Nations Nationalities Peoples Region (SNNPR)] in June 2015. A total of 1839 households were selected using multi-stage sampling procedures. The household respondents were the heads of households. A questionnaire was administered and the data were captured electronically. STATA software version 12 was used to analyse the data. Survey commands were used to account for the multi-stage sampling approach. Household descriptive statistics related to characteristics and levels of knowledge and perception on net care and repair are presented. Ordinal logistic regression was used to identify factors associated with net care and repair perceptions. RESULTS: Less than a quarter of the respondents (22.3%: 95% CI 20.4-24.3%) reported adequate knowledge of net care and repair; 24.6% (95% CI 22.7-26.5%) of the respondents reported receiving information on net care and repair in the previous 6 months. Thirty-five per cent of the respondents (35.1%: 95% CI 32.9-37.4%) reported positive perceptions towards net care and repair. Respondents with adequate knowledge on net care and repair (AOR 1.58: 95% CI 1.2-2.02), and those who discussed net care and repair with their family (AOR 1.47: 95% CI 1.14-1.89) had higher odds of having positive perceptions towards net care and repair. CONCLUSIONS: The low level of reported knowledge on net care and repair, as well as the low level of reported positive perception towards net repair need to be addressed. Targeted behavioural change communication campaigns could be used to target specific groups; increased net care and repair would lead to longer lasting nets

Antimicrobial resistance surveillance in Ethiopia: Implementation experiences and lessons learned

Introduction: Antimicrobial resistance (AMR) poses a global threat. High levels of AMR to commonly used antibiotics have been reported in East Africa. A situation analysis of AMR in Ethiopia also indicated high resistance levels. To prevent and contain AMR, Ethiopia established a national surveillance network. Objectives: This article describes the steps taken to prioritise AMR and establish the National Antimicrobial Resistance Surveillance System in Ethiopia, as well as present the challenges and lessons learned through implementation. Methods: In April 2017, Ethiopia had developed and approved the National AMR Surveillance Plan for laboratory-based AMR surveillance. The World Health Organization recommendations and Ethiopias’s current microbiology capacity were used to prioritise organisms for reporting. The surveillance system is comprised of a network linking the national reference laboratory with surveillance sentinel sites. Roll-out of the AMR surveillance network occurred in three phases in order to ensure successful implementation. Results: Electronic capture and transmission of data, supply chain for the microbiology laboratory and communication problems were challenges observed after implementation started. Support from Ethiopian Public Health Institute focal persons for data entry, regular scheduled communication establishment and procurement of supplies by the American Society for Microbiology were some of the measures taken to address the challenges. Conclusion: Ethiopia has demonstrated that setting up AMR surveillance in lower resource settings is possible with strong leadership and stakeholder engagement

Substance use and its predictors among undergraduate medical students of Addis Ababa University in Ethiopia

<p>Abstract</p> <p>Background</p> <p>Substance use remains high among Ethiopian youth and young adolescents particularly in high schools and colleges. The use of alcohol, <it>khat </it>and tobacco by college and university students can be harmful; leading to decreased academic performance, increased risk of contracting HIV and other sexually transmitted diseases. However, the magnitude of substance use and the factors associated with it has not been investigated among medical students in the country. This study was conducted to determine the prevalence of substance use and identify factors that influenced the behavior among undergraduate medical students of Addis Ababa University in Ethiopia.</p> <p>Methods</p> <p>A cross-sectional study using a pre-tested structured self-administered quantitative questionnaire was conducted in June 2009 among 622 medical students (Year I to Internship program) at the School of Medicine. The data were entered into Epi Info version 6.04d and analyzed using SPSS version 15 software program. Descriptive statistics were used for data summarization and presentation. Differences in proportions were compared for significance using Chi Square test, with significance level set at p < 0.05. Multivariate logistic regression analyses were used to assess the magnitude of associations between substance use and socio-demographic and behavioral correlates.</p> <p>Results</p> <p>In the last 12 months, alcohol was consumed by 22% (25% males vs. 14% females, p = 0.002) and <it>khat </it>use was reported by 7% (9% males vs. 1.5% females, p < 0.001) of the students. About 9% of the respondents (10.6% males vs. 4.6% females, p = 0.014) reported ever use of cigarette smoking, and 1.8% were found to be current smokers. Using multiple logistic regression models, being male was strongly associated with alcohol use in the last 12 months (adjusted OR = 2.14, 95% CI = 1.22-3.76). Students whose friends currently consume alcohol were more likely to consume alcohol (adjusted OR = 2.47, 95% CI = 1.50-4.08) and whose friends' use tobacco more likely to smoke (adjusted OR = 3.89, 95% CI = 1.83-8.30). <it>Khat </it>use within the past 12 months was strongly and positively associated with alcohol consumption (adjusted OR = 15.11, 95% CI = 4.24-53.91). Similarly, ever use of cigarette was also significantly associated with alcohol consumption (adjusted OR = 8.65, 95% CI = 3.48-21.50).</p> <p>Conclusions</p> <p>Concordant use of alcohol, <it>khat </it>and tobacco is observed and exposure to friends' use of substances is often implicated. Alcohol consumption or <it>khat </it>use has been significantly associated with tobacco use. While the findings of this study suggest that substance use among the medical students was not alarming, but its trend increased among students from Year I to Internship program. The university must be vigilant in monitoring and educating the students about the consequences of substance use.</p