Grape seed extract reduces the severity of selected disease markers in the proximal colon of dextran sulphate sodium-induced colitis in rats

BackgroundGrape seed extract (GSE) constitutes a rich source of procyanidins. GSE has been demonstrated to exert encouraging anti-inflammatory and anti-ulcer properties in experimental settings, although its effects on inflammation of the colon remain undefined.AimTo determine the effects of GSE in a rat model of dextran sulphate sodium (DSS) for ulcerative colitis.MethodsMale Sprague-Dawley rats were gavaged daily (days 0-10) with GSE (400 mg/kg). Ulcerative colitis was induced by substituting DSS (2 % w/v) for drinking water from days 5-10. A sucrose breath test was performed on day 11 to determine small bowel function and intestinal tissues were collected for histological analyses. Statistical analysis was by one-way or repeated-measures ANOVA and p ResultsCompared to DSS-treated controls, GSE significantly decreased ileal villus height (14 %; p ConclusionsGSE decreased the severity of selected markers of DSS-induced colitis in the distal ileum and proximal colon, suggesting the potential as an adjuvant therapy for the treatment of ulcerative colitis. Future studies of GSE should investigate alternative delivery methods and treatment regimens, further seeking to identify the individual bioactive factors.Ker Y. Cheah, Susan E. P. Bastian, Thomas M. V. Acott, Suzanne M. Abimosleh, Kerry A. Lymn, Gordon S. Howart

The first 80-hour continuous lidar campaign for simultaneous observation ofmesopause region temperature and wind

The Colorado State Sodium lidar has been upgraded to a two-beam system capable of simultaneous measurement of mesopause region temperature and winds, day and night, weather permitting. This paper reports the initial result of the first campaign, conducted in April 2002, with a total of 145 hours of observation including an 80-hour continuous data acquisition of temperature and zonal wind. The contour plots of the continuous data set show considerable coherence and activities of upward propagating waves, with a maximum day-night difference of 15.5 m/s in zonal wind at 88 km and of 10 K in temperature at 92 km. Oscillations at periods of 10-hour in temperature and 16-hour in zonal wind, implicating nonlinear interactions, can be identified. Decomposition of the time series into tidal periods, resulted in very good agreement with the GSWM00 predictions of diurnal tide. The observed altitude dependence in diurnal amplitudes and phases is consistent with the presence of a significant upward propagating wave, accompanying and modulating the main diurnal tide

Tidal perturbations and variability in mesopause region over Fort Collins, CO(41N, 105W): continuous multi-day temperature and wind lidar observations

An unusually long data set was acquired at the sodium lidar facility at Colorado State University (41N, 105W), between Sep 18 and Oct 01, 2003, including a 9-day continuous observation. This time is long enough to average out the perturbations of gravity waves and short-period planetary waves. As such, it can be used to define tidal-period perturbations in temperature and horizontal wind. Assuming the sodium mixing ratio is a constant of motion, the observed tidal-period oscillation in sodium density follows that of vertical wind. Thus, the data set defines tidal-period perturbations of temperature and wind vector. The observed amplitudes and phases were compared to Global Scale Wave Model predictions (both GSWM00 and GSWM02). We found excellent agreement in diurnal phases and reasonable agreement in semidiurnal phases. However, GSWM02 overestimates diurnal amplitudes and both model versions underestimate observed semidiurnal amplitudes. Since the data period is long enough for the study of planetary waves and of tidal variability, we perform spectral analysis of the data, revealing a strong quasi 3-day wave in meridional wind, a 14 hour perturbation in zonal wind, and both 14-hour and 10-hour periods in meridional wind, likely the result of nonlinear interactions. The observed semidiurnal amplitudes are much larger than the corresponding diurnal amplitudes above 85 km, and over a few days the diurnal and semidiurnal amplitudes vary by factors of 2–3. Causes for the observed tidal variability in terms of planetary wave modulation and tide-gravity wave interaction are explored qualitatively

Seasonal variation of diurnal perturbations in mesopause regiontemperature, zonal, and meridional winds above Fort Collins, Colorado (40.6°N, 105°)

On the basis of lidar observations from May 2002 through April 2003, covering both day and night, we performed a harmonic analysis to extract the diurnal perturbations in mesopause region temperature, zonal and meridional winds over Fort Collins, Colorado (40.6°N, 105°W), binned every 2 months. The results were compared to predictions of the 2000 and 2002 versions of Global-Scale Wave Model (GSWM00 and GSWM02). The diurnal tidal period oscillations showed a mixture of propagating and evanescent (trapped) modes, but the propagating modes dominated for most of the year. The agreement in temperature diurnal phases between observation and GSWM prediction is marginal. On the other hand, other than July-August meridional winds, the observed diurnal phases in both wind components are in good agreement with GSWM predictions for most of the altitude range reported. The diurnal amplitude predictions of GSWM00 were reasonably close to lidar observations, while other than January-February, the GSWM02 amplitude prediction overestimated the observations, typically by a factor of two. We also conducted comparisons on tidal perturbations in zonal wind between radar campaigns and our lidar observations. The lidar data agreed reasonably well with the MF radar data from 2000 to 2001 at nearby Platteville, Colorado (40.2°N, 104.7°W), but showed considerable differences with the data from other midlatitude stations from 1992 to 1993. The dominance of the evanescent mode in the temperature diurnal tidal oscillation during the early winter (November and December), which reached a peak value at midnight, was interesting and anomalous. By invoking the more recent data (November and December in 2003), as well as the diurnal temperature observations from December 1998, we report that the evanescent (trapped) diurnal tidal perturbations were robust and persisted from one year to the next

Purification and Characterization of a Sperm Motility Inhibiting Factor from Caprine Epididymal Plasma

Several studies have been reported on the occurrence of sperm motility inhibiting factors in the male reproductive fluids of different mammalian species, but these proteins have not been adequately purified and characterized. A novel sperm motility inhibiting factor (MIF-II) has been purified from caprine epididymal plasma (EP) by Hydroxylapatite gel adsorption chromatography, DEAE-Cellulose ion-exchange chromatography and chromatofocusing. The MIF-II has been purified to apparent homogeneity and the molecular weight estimated by Sephacryl S-300 gel filtration is 160 kDa. MIF-II is a dimeric protein, made up of two subunits each having a molecular mass of 80 kDa as shown by SDS-PAGE. The isoelectric point of MIF-II is 5.1 as determined by chromatofocusing and isoelectric focusing. It is a heat labile protein and maximal active at the pH 6.9 to 7.5. The sperm motility inhibiting protein factor at 2 µg/ml (12.5 nM) level showed maximal motility-inhibiting activity. The observation that the epididymal plasma factor lowered the intracellular cAMP level of spermatozoa in a concentration-dependent manner suggests that it may block the motility of caprine cauda spermatozoa by interfering the cAMP dependent motility function. The results revealed that the purified protein factor has the potential of sperm motility inhibition and may serve as a vaginal contraceptive. The antibody raised against the MIF-II has the potential for enhancement of forward motility of cauda-spermatozoa. This antibody may thus be useful for solving some of the problems of male infertility due to low sperm motility

Charting the course for a Blue Economy in Peru: A Research Agenda

Ocean- and coastal-based economic activities are increasingly recognised as key drivers for supporting global economies. This move towards the “blue economy” is becoming globally widespread, with the recognition that if ocean-based activities are to be sustainable, they will need to move beyond solely extractive and exploitative endeavours, aligning more closely with marine conservation and effective marine spatial planning. In this paper we define the “blue economy” as a “platform for strategic, integrated and participatory coastal and ocean development and protection that incorporates a low carbon economy, the ecosystem approach and human well-being through advancing regional industries, services and activities”. In Peru, while the seas contribute greatly to the national economy, the full potential of the blue economy has yet to be realised. This paper presents the findings of an early career scientist workshop in Lima, Peru, in March 2016. The workshop “Advancing Green Growth in Peru” brought together researchers to identify challenges and opportunities for green growth across three Peruvian economic sectors—tourism, transport and the blue economy with this paper exploring in detail the priorities generated from the “blue economy” stream. These priorities include themes such as marine spatial planning, detailed evaluations of existing maritime industries (e.g. guano collection and fisheries), development of an effective MPA network, support for sustainable coastal tourism, and better inclusion of social science disciplines in understanding societal and political support for a Peruvian blue economy. In addition, the paper discusses the research requirements associated with these priorities. While not a comprehensive list, these priorities provide a starting point for future dialogue on a co-ordinated scientific platform supporting the blue growth agenda in Peru, and in other regions working towards a successful “blue economy”

Mapping of the Disease Locus and Identification of ADAMTS10 As a Candidate Gene in a Canine Model of Primary Open Angle Glaucoma

Primary open angle glaucoma (POAG) is a leading cause of blindness worldwide, with elevated intraocular pressure as an important risk factor. Increased resistance to outflow of aqueous humor through the trabecular meshwork causes elevated intraocular pressure, but the specific mechanisms are unknown. In this study, we used genome-wide SNP arrays to map the disease gene in a colony of Beagle dogs with inherited POAG to within a single 4 Mb locus on canine chromosome 20. The Beagle POAG locus is syntenic to a previously mapped human quantitative trait locus for intraocular pressure on human chromosome 19. Sequence capture and next-generation sequencing of the entire canine POAG locus revealed a total of 2,692 SNPs segregating with disease. Of the disease-segregating SNPs, 54 were within exons, 8 of which result in amino acid substitutions. The strongest candidate variant causes a glycine to arginine substitution in a highly conserved region of the metalloproteinase ADAMTS10. Western blotting revealed ADAMTS10 protein is preferentially expressed in the trabecular meshwork, supporting an effect of the variant specific to aqueous humor outflow. The Gly661Arg variant in ADAMTS10 found in the POAG Beagles suggests that altered processing of extracellular matrix and/or defects in microfibril structure or function may be involved in raising intraocular pressure, offering specific biochemical targets for future research and treatment strategies

Trabecular Meshwork Gene Expression after Selective Laser Trabeculoplasty

BACKGROUND: Trabecular meshwork and Schlemm's canal are the tissues appointed to modulate the aqueous humour outflow from the anterior chamber. The impairment of their functions drives to an intraocular pressure increase. The selective laser trabeculoplasty is a laser therapy of the trabecular meshwork able to decrease intraocular pressure. The exact response mechanism to this treatment has not been clearly delineated yet. The herein presented study is aimed at studying the gene expression changes induced in trabecular meshwork cells by selective laser trabeculoplasty (SLT) in order to better understand the mechanisms subtending its efficacy. METHODOLOGY/PRINCIPAL FINDINGS: Primary human trabecular meshwork cells cultured in fibroblast medium underwent selective laser trabeculoplasty treatment. RNA was extracted from a pool of cells 30 minutes after treatment while the remaining cells were further cultured and RNA was extracted respectively 2 and 6 hours after treatment. Control cells stored in incubator in absence of SLT treatment were used as reference samples. Gene expression was evaluated by hybridization on miRNA-microarray and laser scanner analysis. Scanning electron microscopic examination was performed on 2 Trabecular meshwork samples after SLT at 4(th) and 6(th) hour from treatment. On the whole, selective laser trabeculoplasty modulates in trabecular meshwork the expression of genes involved in cell motility, intercellular connections, extracellular matrix production, protein repair, DNA repair, membrane repair, reactive oxygen species production, glutamate toxicity, antioxidant activities, and inflammation. CONCLUSIONS/SIGNIFICANCE: SLT did not induce any phenotypic alteration in TM samples. TM is a complex tissue possessing a great variety of function pivotal for the active regulation of aqueous humour outflow from the anterior chamber. SLT is able to modulate these functions at the postgenomic molecular level without inducing damage either at molecular or phenotypic levels

CXCR3 Antagonism of SDF-1(5-67) Restores Trabecular Function and Prevents Retinal Neurodegeneration in a Rat Model of Ocular Hypertension

Glaucoma, the most common cause of irreversible blindness, is a neuropathy commonly initiated by pathological ocular hypertension due to unknown mechanisms of trabecular meshwork degeneration. Current antiglaucoma therapy does not target the causal trabecular pathology, which may explain why treatment failure is often observed. Here we show that the chemokine CXCL12, its truncated form SDF-1(5-67), and the receptors CXCR4 and CXCR3 are expressed in human glaucomatous trabecular tissue and a human trabecular cell line. SDF-1(5-67) is produced under the control of matrix metallo-proteinases, TNF-α, and TGF-β2, factors known to be involved in glaucoma. CXCL12 protects in vitro trabecular cells from apoptotic death via CXCR4 whereas SDF-1(5-67) induces apoptosis through CXCR3 and caspase activation. Ocular administration of SDF-1(5-67) in the rat increases intraocular pressure. In contrast, administration of a selective CXCR3 antagonist in a rat model of ocular hypertension decreases intraocular pressure, prevents retinal neurodegeneration, and preserves visual function. The protective effect of CXCR3 antagonism is related to restoration of the trabecular function. These data demonstrate that proteolytic cleavage of CXCL12 is involved in trabecular pathophysiology, and that local administration of a selective CXCR3 antagonist may be a beneficial therapeutic strategy for treating ocular hypertension and subsequent retinal degeneration

Co-constructing cultural ecosystem services and wellbeing through a place-based approach

Reductive practices in fisheries management have tended to focus on ecological and economic dimensions that have rendered the social and cultural importance of fishing largely invisible, at least in the context of governance and policy making. This chapter builds on 5 years’ research in the English Channel and Southern North Sea in which the authors adopted a sense of place perspective as a framework for understanding the social and cultural value of small-scale fisheries. Through a number of case studies, the chapter describes how small-scale fisheries result in a series of ‘transformations’ as the marine environment is translated into cultural ecosystem services in coastal settings giving rise to socio-cultural value. This perspective is further developed by considering the value of the social wellbeing ‘lens’ to broaden the sense of place / cultural ecosystem services framework. In pursuing ‘values’ through sense of place, cultural ecosystem services and social wellbeing we discuss how the dualistic treatment of nature and society is problematic. We conclude that a relational co-constructionist approach, although challenging, offers a way of making visible an array of social and cultural values that emerge from the activity of small-scale fisheries