PlanetCam UPV/EHU: a two-channel lucky imaging camera for solar system studies in the spectral range 0.38-1.7 µm

This is an author-created, un-copyedited version of an article published in Publications of the Astronomical Society of the Pacific. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it.We present PlanetCam UPV/EHU, an astronomical camera designed fundamentally for high-resolution imaging of Solar System planets using the “lucky imaging” technique. The camera observes in a wavelength range from 380 nm to 1.7 µm and the driving science themes are atmosphere dynamics and vertical cloud structure of Solar System planets. The design comprises two configurations that include one channel (visible wavelengths) or two combined channels (visible and short wave nfrared) working simultaneously at selected wavelengths by means of a dichroic beam splitter. In this paper the camera components for the two configurations are described, as well as camera performance and the different tests done for the precise characterization of its radiometric and astrometric capabilities at high spatial resolution. Finally, some images of solar system objects are presented as well as photometric results and different scientific cases on astronomical targets.Peer ReviewedPostprint (author's final draft

Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept

Objective: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. Approach: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380–420 nm illumination at angles similar to those of maximal DLP. Per-pixel image analysis included quantification and combination of polarization feature descriptors in 475 samples. Main results: The angles (from sample surface) of maximal DLP were 3° for 405 nm and 6° for 514 nm. Similar viral particles that differed only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned at 10-degree and 15-degree configurations. Significance: Polarimetric imaging in the visible spectrum may help improve fast, non-contact detection and identification of viral particles, and/or other microbes such as tuberculosis, in multiple dry fluid samples simultaneously, particularly when combined with other imaging modalities. Further analysis including realistic concentrations of real SARS-CoV-2 viral particles in relevant human fluids is required. Polarimetric imaging under visible light may contribute to a fast, cost-effective screening of SARS-CoV-2 and other pathogens when combined with other imaging modalities.12 página

Hyperspectral image processing for the identification and quantification of lentiviral particles in fluid samples

Optical spectroscopic techniques have been commonly used to detect the presence of biofilm-forming pathogens (bacteria and fungi) in the agro-food industry. Recently, near-infrared (NIR) spectroscopy revealed that it is also possible to detect the presence of viruses in animal and vegetal tissues. Here we report a platform based on visible and NIR (VNIR) hyperspectral imaging for non-contact, reagent free detection and quantification of laboratory-engineered viral particles in fluid samples (liquid droplets and dry residue) using both partial least square-discriminant analysis and artificial feed-forward neural networks. The detection was successfully achieved in preparations of phosphate buffered solution and artificial saliva, with an equivalent pixel volume of 4 nL and lowest concentration of 800 TU.mu L-1. This method constitutes an innovative approach that could be potentially used at point of care for rapid mass screening of viral infectious diseases and monitoring of the SARS-CoV- 2 pandemic.This research was funded by grants number COV20-00080 and COV20-00173 of the 2020 Emergency Call for Research Projects about the SARS-CoV-2 virus and the COVID-19 disease of the Institute of Health 'Carlos III', Spanish Ministry of Science and Innovation, and by grant number EQC2019-006240-P of the 2019 Call for Acquisition of Scientific Equipment, FEDER Program, Spanish Ministry of Science and Innovation. This work has been supported by the European Commission through the JRC HUMAINT project. ABR was supported by grant number RTI2018-094465-J funded by the Spanish National Agency of Research. The authors would like to gratefully acknowledge the assistance of the members of the EOD-CBRN Group of the Spanish National Police, whose identities cannot be disclosed, and who are represented here by JMNG. Authors thank continuous support from their institutions

PlanetCam UPV/EHU: a two-channel lucky imaging camera for solar system studies in the spectral range 0.38-1.7 µm

This is an author-created, un-copyedited version of an article published in Publications of the Astronomical Society of the Pacific. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it.We present PlanetCam UPV/EHU, an astronomical camera designed fundamentally for high-resolution imaging of Solar System planets using the “lucky imaging” technique. The camera observes in a wavelength range from 380 nm to 1.7 µm and the driving science themes are atmosphere dynamics and vertical cloud structure of Solar System planets. The design comprises two configurations that include one channel (visible wavelengths) or two combined channels (visible and short wave nfrared) working simultaneously at selected wavelengths by means of a dichroic beam splitter. In this paper the camera components for the two configurations are described, as well as camera performance and the different tests done for the precise characterization of its radiometric and astrometric capabilities at high spatial resolution. Finally, some images of solar system objects are presented as well as photometric results and different scientific cases on astronomical targets.Peer Reviewe