132 research outputs found
The formation of ethnically mixed partnerships in Estonia: A stalling trend from a two-sided perspective
BACKGROUNDEthnically mixed partnerships are often regarded as the ultimate evidence of the integration of migrants and their descendants into their host society. A common finding in the literature is an increase in the occurrence of mixed partnerships across migrant generations.OBJECTIVEThis study investigates the formation of minority-majority partnerships in Estonia, with special attention to the variation associated with the migrants' generation and their exposure to the majority population.METHODSThe study uses pooled data from the Estonian Family and Fertility Survey (FFS) and the Estonian Generations and Gender Survey (GGS), and estimates proportional hazards models.RESULTSThe experience of second-generation migrants indicates a stalling trend in the incidence of mixed partnerships between the majority population and migrant groups, which is rooted in contextual features. Apart from residential proximity, the study shows the salience of early acquisition of the host society language. Our results for the majority population highlight the role of international migration, which exposes host populations to mixed partnership formation.CONCLUSIONSThe results lend support to the view that the integration of migrant populations through mixed partnering is a lengthy process that stretches across several generations. A linguistically divided school system and residential segregation contribute to the pillarization of society
Surprising variations in the rotation of the chemically peculiar stars CU Virginis and V901 Orionis
CU Vir and V901 Ori belong among these few magnetic chemically peculiar stars
whose rotation periods vary on timescales of decades. We aim to study the
stability of the periods in CU Vir and V901 Ori using all accessible
observational data containing phase information. We collected all available
relevant archived observations supplemented with our new measurements of these
stars and analysed the period variations of the stars using a novel method that
allows for the combination of data of diverse sorts. We found that the shapes
of their phase curves were constant, while the periods were changing. Both
stars exhibit alternating intervals of rotational braking and acceleration. The
rotation period of CU Vir was gradually shortening until the year 1968, when it
reached its local minimum of 0.52067198 d. The period then started increasing,
reaching its local maximum of 0.5207163 d in the year 2005. Since that time the
rotation has begun to accelerate again. We also found much smaller period
changes in CU Vir on a timescale of several years. The rotation period of V901
Ori was increasing for the past quarter-century, reaching a maximum of 1.538771
d in the year 2003, when the rotation period began to decrease. A theoretically
unexpected alternating variability of rotation periods in these stars would
remove the spin-down time paradox and brings a new insight into structure and
evolution of magnetic upper-main-sequence stars.Comment: 5 pages, 3 figure
Random, blocky and alternating ordering in supramolecular polymers of chemically bidisperse monomers
As a first step to understanding the role of molecular or chemical
polydispersity in self-assembly, we put forward a coarse-grained model that
describes the spontaneous formation of quasi-linear polymers in solutions
containing two self-assembling species. Our theoretical framework is based on a
two-component self-assembled Ising model in which the bidispersity is
parameterized in terms of the strengths of the binding free energies that
depend on the monomer species involved in the pairing interaction. Depending
upon the relative values of the binding free energies involved, different
morphologies of assemblies that include both components are formed, exhibiting
paramagnetic-, ferromagnetic- or anti ferromagnetic-like order,i.e., random,
blocky or alternating ordering of the two components in the assemblies.
Analyzing the model for the case of ferromagnetic ordering, which is of most
practical interest, we find that the transition from conditions of minimal
assembly to those characterized by strong polymerization can be described by a
critical concentration that depends on the concentration ratio of the two
species. Interestingly, the distribution of monomers in the assemblies is
different from that in the original distribution, i.e., the ratio of the
concentrations of the two components put into the system. The monomers with a
smaller binding free energy are more abundant in short assemblies and monomers
with a larger binding affinity are more abundant in longer assemblies. Under
certain conditions the two components congregate into separate supramolecular
polymeric species and in that sense phase separate. We find strong deviations
from the expected growth law for supramolecular polymers even for modest
amounts of a second component, provided it is chemically sufficiently distinct
from the main one.Comment: Submitted to Macromolecules, 6 figures. arXiv admin note: substantial
text overlap with arXiv:1111.176
Determination of Membrane Protein Transporter Oligomerization in Native Tissue Using Spatial Fluorescence Intensity Fluctuation Analysis
Membrane transporter proteins exist in a complex dynamic equilibrium between various oligomeric states that include monomers, dimers, dimer of dimers and higher order oligomers. Given their sub-optical microscopic resolution size, the oligomerization state of membrane transporters is difficult to quantify without requiring tissue disruption and indirect biochemical methods. Here we present the application of a fluorescence measurement technique which combines fluorescence image moment analysis and spatial intensity distribution analysis (SpIDA) to determine the oligomerization state of membrane proteins in situ. As a model system we analyzed the oligomeric state(s) of the electrogenic sodium bicarbonate cotransporter NBCe1-A in cultured cells and in rat kidney. The approaches that we describe offer for the first time the ability to investigate the oligomeric state of membrane transporter proteins in their native state
A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans
This is the first of a series of calorimetric studies designed to characterize and understand survival mechanisms of metal-reducing bacteria isolated from metal-polluted environments. In this paper we introduce a new concept of thermal spectrum of the endothermic melting of complex biological systems (e.g., proteins, nucleic acids, ribosomes, membrane structures) in intact cells. All thermal spectra measured are thermograms that describe the temperature dependence of heat capacity change of the complex systems of biologically active substances in bacterial cells. This new concept of thermal spectrum was applied to investigate spectral features from intact cells of Cr(VI)-reducer Arthrobacter oxydans at different points of their growth conditions and stages. Over the temperature range of 40–105°C, we observed that spectral changes are particularly significant in the 40–90°C interval. This may correspond to the orderly changes in subcellular structural elements: proteins, ribosomes and RNA, membranes, and various structural elements of the cell wall during different points of the growth cycle and growth conditions. Spectral changes in the 90–105°C region are less pronounced, implicating that the structural composition of DNA-Protein (DNP) complexes may change little
Genomic, Proteomic and Physiological Characterization of a T5-like Bacteriophage for Control of Shiga Toxin-Producing Escherichia coli O157:H7
Despite multiple control measures, Escherichia coli O157:H7 (STEC O157:H7) continues to be responsible for many food borne outbreaks in North America and elsewhere. Bacteriophage therapy may prove useful for controlling this pathogen in the host, their environment and food. Bacteriophage vB_EcoS_AKFV33 (AKFV33), a T5-like phage of Siphoviridae lysed common phage types of STEC O157:H7 and not non-O157 E. coli. Moreover, STEC O157:H7 isolated from the same feedlot pen from which the phage was obtained, were highly susceptible to AKFV33. Adsorption rate constant and burst size were estimated to be 9.31×10−9 ml/min and 350 PFU/infected cell, respectively. The genome of AKVF33 was 108,853 bp (38.95% G+C), containing 160 open reading frames (ORFs), 22 tRNA genes and 32 strong promoters recognized by host RNA polymerase. Of 12 ORFs without homologues to T5-like phages, 7 predicted novel proteins while others exhibited low identity (<60%) to proteins in the National Centre for Biotechnology Information database. AKVF33 also lacked the L-shaped tail fiber protein typical of T5, but was predicted to have tail fibers comprised of 2 novel proteins with low identity (37–41%) to tail fibers of E. coli phage phiEco32 of Podoviridae, a putative side tail fiber protein of a prophage from E. coli IAI39 and a conserved domain protein of E. coli MS196-1. The receptor-binding tail protein (pb5) shared an overall identify of 29–72% to that of other T5-like phages, with no region coding for more than 6 amino acids in common. Proteomic analysis identified 4 structural proteins corresponding to the capsid, major tail, tail fiber and pore-forming tail tip (pb2). The genome of AKFV33 lacked regions coding for known virulence factors, integration-related proteins or antibiotic resistance determinants. Phage AKFV33 is a unique, highly lytic STEC O157:H7-specific T5-like phage that may have considerable potential as a pre- and post-harvest biocontrol agent
Effect of Systemic Hypertension With Versus Without Left Ventricular Hypertrophy on the Progression of Atrial Fibrillation (from the Euro Heart Survey).
Hypertension is a risk factor for both progression of atrial fibrillation (AF) and development of AF-related complications, that is major adverse cardiac and cerebrovascular events (MACCE). It is unknown whether left ventricular hypertrophy (LVH) as a consequence of hypertension is also a risk factor for both these end points. We aimed to assess this in low-risk AF patients, also assessing gender-related differences. We included 799 patients from the Euro Heart Survey with nonvalvular AF and a baseline echocardiogram. Patients with and without hypertension were included. End points after 1 year were occurrence of AF progression, that is paroxysmal AF becoming persistent and/or permanent AF, and MACCE. Echocardiographic LVH was present in 33% of 379 hypertensive patients. AF progression after 1 year occurred in 10.2% of 373 patients with rhythm follow-up. In hypertensive patients with LVH, AF progression occurred more frequently as compared with hypertensive patients without LVH (23.3% vs 8.8%, p = 0.011). In hypertensive AF patients, LVH was the most important multivariably adjusted determinant of AF progression on multivariable logistic regression (odds ratio 4.84, 95% confidence interval 1.70 to 13.78, p = 0.003). This effect was only seen in male patients (27.5% vs 5.8%, p = 0.002), while in female hypertensive patients, no differences were found in AF progression rates regarding the presence or absence of LVH (15.2% vs 15.0%, p = 0.999). No differences were seen in MACCE for hypertensive patients with and without LVH. In conclusion, in men with hypertension, LVH is associated with AF progression. This association seems to be absent in hypertensive women
Progression From Paroxysmal to Persistent Atrial Fibrillation. Clinical Correlates and Prognosis
Objectives: We investigated clinical correlates of atrial fibrillation (AF) progression and evaluated the prognosis of patients demonstrating AF progression in a large population. Background: Progression of paroxysmal AF to more sustained forms is frequently seen. However, not all patients will progress to persistent AF. Methods: We included 1,219 patients with paroxysmal AF who participated in the Euro Heart Survey on AF and had a known rhythm status at follow-up. Patients who experienced AF progression after 1 year of follow-up were identified. Results: Progression of AF occurred in 178 (15%) patients. Multivariate analysis showed that heart failure, age, previous transient ischemic attack or stroke, chronic obstructive pulmonary disease, and hypertension were the only independent predictors of AF progression. Using the regression coefficient as a benchmark, we calculated the HATCH score. Nearly 50% of the patients with a HATCH score >5 progressed to persistent AF compared with only 6% of the patients with a HATCH score of 0. During follow-up, patients with AF progression were more often admitted to the hospital and had more major adverse cardiovascular events. Conclusions: A substantial number of patients progress to sustained AF within 1 year. The clinical outcome of these patients regarding hospital admissions and major adverse cardiovascular events was worse compared with patients demonstrating no AF progression. Factors known to cause atrial structural remodeling (age and underlying heart disease) were independent predictors of AF progression. The HATCH score may help to identify patients who are likely to progress to sustained forms of AF in the near future. \ua9 2010 American College of Cardiology Foundation
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