SKS splittings in the southern Apennines-Calabrian arc region (southern Italy)

During the years 2003-2006 CAT/SCAN (Calbarian-Apennine-TyrrhenianlSubductionCollision- Accretion Network) temporary broadband stations operate in Southern Apennine and Calabria (Italy). In the same period CESIS-INGV project improved the number of permanent seismic stations in the same area. We analyze the data recorded to study seismic anisotropy and to investigate the mantle flow in the boun(fary-zoile{ between Southern Apennine and Calabriaibeneath and above the subducting slab. In the current work we present new shear wave splittings obtained analyzing SKS phases of 15 teleseisms with epicentral distance ranging from 88.40 to 98.20 and magnitude greater than 6.0. We used the method of Silver & Chan (1991) to obtained anisotropic parameters: delay time and fast polarization direction. The splitting parameters reveal strong seismic anisotropy in the mantle beneath Southern Tyrrhenian Sea- Calabrian Arc System that seems to be controlled by the slab presence. The clear variability in the fast directions allow us to hypothesize the existence of different anisotropic domains: fast polarization directions vary from NNW -SSE in the tyrrhenian side ofthe Southern Apennine to N-S and NE-SW toward the Adriatic Sea. Moving toward the Calabria fast directions are prevalently trench parallel showing a NE-SW orientation following the strike on the mountain chain

Transmembrane transporters and salt tolerance in temperate japonica rice

Several investigations aimed at identifying molecular tools useful for the selection and/or the constitution of high-yield salt tolerant rice have been successfully carried out, concerning in particular indica and/ or tropical rice genotypes. Te global warming process is nowadays determining the intrusion of saline wedge into coastal fresh-water streams, and the soil salt concentration of many European rice areas, where temperate rice cultivars are mainly grown, is more and more increasing. In order to identify molecular markers and/or new loci related to salt tolerance, a Genome Wide Association Study (GWAS) has been carried out using a panel of 277 japonica rice accessions. Te panel has been subjected to Genotyping By Sequencing and phenotyping concerning tolerance to a mild-salt stress soil condition (5 dS m-1) expressed at the 4th-5th leaf developmental stage using the Standard Evaluation Score (SES) proposed by IRRI. On the basis of GWAS, a QTL including a few genes that in the indica rice genome are localized within the major salinity tolerance-related QTL \u2018SalTol\u2019 have been identified. Among them, the Os01g0337500 gene encoding the vacuolar H+-pyrophosphatase 6 (OsOVP6) is present. Since the role of the OsOVP6 activity is considered central in regulating the cellular Na+ homeostasis in both roots and leaves, investigations comparing some elements of the complex mechanisms involved in this process have been carried out. A physiological approach evaluating this possibility has been conducted in two japonica rice varieties (Galileo and Virgo) that resulted salt-tolerant, in one japonica rice variety (PL12) known to be quite salt-susceptible, and in the salt-tolerant indica inbred genotype FL478 (containing the \u2018SalTol\u2019 QTL) as reference genotype. Te root and shoot Na+/K+ ratio, Na+ influx and K+ efflux, H+ extrusion activity, cytosolic and vacuolar pH by in vivo 31P-NMR techniques were evaluated in roots of the four rice genotypes. Te results obtained, together with the electrophysiological evaluation of the whole root Na+ conductance, allow to define a picture that may explain the different salt tolerance observed among the rice genotypes analyzed. As a whole, these results confirm the interest towards a deep allele mining analysis, concerning OsOVP6, within the most significant members of the japonica rice accession panel under investigation

Shear wave splitting in southern tyrrhenian subduction zone (Italy) from CESIS and CAT/SCAN projects

In the years 2003 -2006 several broad band stations were installed in Southern Italy: 15 permanent ones (CESIS project), improved the INGV Italian national network and 40 temporary ones were installed in the frame of CAT/SCAN NSF project.We present shear wave splitting measurements obtained analyzing SKS phases and local S phases from slab earthquakes. We used the method of Silver & Chan to obtain shear wave splitting parameters: fast direction and delay time. Shear wave splitting measurements reveals strong seismic anisotropy in the mantle beneath Southern Tyrrhenian subduction system. The SKS splitting results show fast polarization directions varying from NNW-SSE in the Southern Apennines to N-S and to E-SW in Calabria, following the strike of the mountain chain. Moving toward the Adriatic sea the fast directions rotate from N-S to NE-SW. Fast directions could indicate the mantle flow below the slab, due to its retrograde motion but also the lithospheric fabric of the subducting plate. In the Tyrrhenian domain, above the slab, from Sardinia to the Italian and Sicilian coasts the dominant fast direction is E-W and could be related to the opening of the Tyrrhenian basin and to the corner flow in the asthenospheric wedge. In Sicily fast directions depict a ring around the slab edge supporting the existence of a slab tear and of a return flow from the back to the front of the slab. Measurements obtained with intermediate and deep earthquakes slab S phases show an extremely complex pattern of fast directions. They are mostly distributed in front of the Tyrrhenian Calabrian coast in correspondence of the fast velocity anomaly imaged at 150 km depth by tomography. We can relate this fast directions variability to the complex structure of the slab itself. The complex pattern of SKS and S splitting measurements suggests the presence of local scale mantle flow controled by the motion of an anisotropic slab

Breast cancer and communication: Monocentric experience of a self-assessment questionnaire

Background: The communication of the diagnosis of breast cancer induces to the patient a strong psychological trauma. Radiologists are at the forefront of communicating, either for a biopsy or the probable diagnosis of malignancy. This is a complex task, which requires the knowledge and application of correct “communicative models”, among which the SPIKES protocol rep-resents a fundamental reference. Design and methods: 110 patients, with a history of breast cancer, filled out a questionnaire consisting of six questions: five aimed at defining communication compliance with the SPIKES protocol, the sixth, consisting of six feelings, aimed at the knowledge of the next emotional state. Results: Regarding compliance with various “strategic points” of the SPIKES protocol, questionnaires show that 70% of patients reported no omissions, while the remaining 30% reported omissions relatively to perception (56%), emotions (23%), setting (13%), knowledge (6%) and invitation (2%). The results showed the existence of a correlation between the final emotional state and the correct application of the SPIKES protocol; in fact, patients who reacted with a positive final emotional state-reported greater adherence to the strategic points of the SPIKES protocol. Conclusions: In healthcare, knowing the communicative compliance of a team in giving “bad news” is fundamental, especially in breast cancer. The SPIKES protocol is recognized by the Literature as a fundamental reference able to affect “positively” the emotional state of patients. The proposed questionnaire is a valid tool to identify the weak points of communication and related criticalities, to improve clinical practice

The INGV Mobile Telemetered Network (Re.Mo.Tel.): long-term monitoring analysis.

Since 2008, the Istituto Nazionale di Geofisica e Vulcanologia (INGV) developed a temporary real-time telemetered seismic network infrastructure (Re.Mo.Tel.) to densify the Italian National Seismic Network in epicentral area, thus improving the location of the aftershocks distribution after a mainshock. This infrastructure consists of various mobile and autonomous seismic stations that in group of three are telemetered to a Very Small Aperture Terminal (VSAT). Using a dedicated bandwidth on UHF, Wi-Fi and satellite frequency, the system is able to stream real-time data to the INGV acquisition centers in Rome and Grottaminarda. The deployment of the seismic network is supported by a geographical information system (GIS) that visualizes, for the epicentral area, information about instrumental seismicity, seismic risk, macroseismic felts and territorial data. The April, 6th, 2009 Mw 6.3 L'Aquila destructive earthquake represented the first real-case where the entire Re.Mo.Tel. infrastructure has been deployed. Less than 6 hours after the earthquake occurrence, a first accelerometric station was streaming data to the INGV seismic monitoring headquarters. A total number of 9 seismic stations was installed within 4 days after the main event, with the aim of recording continuous data to contribute in the aftershocks detection. In detail the first day a number of 3 stations was installed, surrounding the mainshock. Then the rest of the stations was deployed on the third and the fourth day to the south and the north, following the post-seismic evolution. A project funded by the Dipartimento di Protezione Civile (DPC-Civil Defense) gave the opportunity to maintain some of the deployed stations throughout the duration of the project of one year (although the stations are still installed) to assure the long-term monitoring of the triggered seismic sequence. In this work, we investigate: • The impact of the Re.Mo.Tel. on earthquakes detection and real-time monitoring of the sequence evolution; • The location improvement due to the deployment of stations closer to the epicentral area (with installations that followed the early evolution and complexity of fault fractures); • The network reliability in terms of data stored throughout the working period

Prostaglandin E2 drives cyclooxygenase-2 expression via cyclic AMP response element activation in human pancreatic cancer cells.

Cyclooxygenase-2 (COX-2) is constitutively expressed in most human primary carcinomas and with its synthesized product, prostaglandin E2 (PGE2), appears to play important roles in tumor invasion, angiogenesis, resistance to apoptosis and suppression of host immunity. However, the molecular mechanisms that control COX-2 expression are unclear. The purpose of this study was to clarify the mechanism of basal and PGE2-mediated COX-2 expression in the highly metastatic L3.6pl human pancreatic cancer cell line. Using RNA interference to disrupt the expression of CREB and the NF-kappaB p65 subunit, we found that both are involved in maintaining basal COX-2 expression in L3.6pl cells. We also demonstrated that PGE2 increased the cyclic AMP concentration, thereby activating protein kinase A (PKA), which in turn phosphorylated the cyclic AMP response element binding protein (CREB), leading to interaction with the cyclic AMP response element in the promoter region of the COX-2 gene. Immunocytochemical analysis confirmed that PGE2 stimulated the translocation of PKA to the nucleus and increased the immuno-reactivity of phosphorylated CREB. Pretreatment with the PKA selective inhibitor H 89 and the E-prostanoid receptor 2 inhibitor AH 6809 reduced COX-2 upregulation by PGE2. Electrophoretic mobility shift assay and chromatin immunoprecipitation assay results further suggested a role for CREB in COX-2 transcriptional control. Understanding the pathways that control COX-2 expression may lead to a better understanding of its dysregulation in pancreatic carcinomas and facilitate the development of novel therapeutic approaches

Sequenze sismiche: La nuova struttura di Pronto Intervento dell’Istituto Nazionale di Geofisica e Vulcanologia

Fin dai primi anni ‘70, l’Istituto Nazionale di Geofisica e Vulcanologia (INGV, ex ING) si avvale di una struttura di Pronto Intervento in occasione di eventi sismici rilevanti garantendo in tempi relativamente brevi il monitoraggio di dettaglio in area epicentrale. Dal suo primo intervento (terremoto di Ancona 1972) ad oggi, la struttura di Pronto Intervento si è evoluta costantemente implementando migliorie tecnologiche e logistiche. In questo lavoro prensentiamo la nuova struttura impiegata per la prima volta durante l’ ”Operazione Blue Mountains 2008”, l'esercitazione di Protezione Civile sul rischio sismico organizzata dalla Regione Marche il 23-25 maggio 2008

Impact on mental health, disease management, and socioeconomic modifications in hematological patients during the COVID-19 pandemic in Italy

Background: Hematological patients are a highly vulnerable population with an increased risk of developing severe COVID-19 symptoms due to their immunocompromised status. COVID-19 has proven to cause serious mental health issues, such as stress, anxiety, and depression in the general population. However, data on the psycho-social impact of COVID-19 on hematological patients are lacking.Objectives: This study aims to examine the psychological well-being of hematological patients in Italy during the initial period of the COVID-19 pandemic. Furthermore, it seeks to explore the association between modifications in the management of hematological diseases and employment status of these patients during the COVID-19 pandemic and the resulting mental health outcomes.Design and Methods: A survey using the DASS-21 questionnaire was administered to 1105 hematological patients. Data analysis was conducted using the R software, and logistic regression analysis was performed to predict the association between hematological patient/general population and employment status with DASS scores.Results: The hematological patient population reported significantly higher levels of depression (OR 0.947, 95% CI 0.966-0.982, p < 0.001), anxiety (OR 0.948, 95% CI 0.939-0.958, p < 0.001), and stress (OR 0.984, 95% CI 0.977-0.992, p < 0.001) compared with the general population. A significant relationship has been found in stress between employed and unemployed patients (OR 1.015, 95% CI 1.000-1.030, p = 0.044), as well as in the control group (OR 1.024, 95% CI 1.010-1.039, p = 0.001). In addition, employment status is significantly related to depression, anxiety, and stress in both the hematological patient group and the general population.Conclusion: During the initial phase of the COVID-19 pandemic, hematological patients had elevated levels of depression, anxiety, and stress compared with the general population. The delay in their treatment and employment status played a role in their mental health outcomes. These findings emphasize the importance of further research to gain deeper insight into the long-term psychological effects and explore effective strategies for managing mental health in similar crises