78 research outputs found

    Factor de riego para nectarino

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    Ministerio de Economía y Competitividad AGL2013-49047-C02-2

    Mahogunin Ring Finger 1 Is Required for Genomic Stability and Modulates the Malignant Phenotype of Melanoma Cells.

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    The mouse mahoganoid mutation abrogating Mahogunin Ring Finger-1 (MGRN1) E3 ubiquitin ligase expression causes hyperpigmentation, congenital heart defects and neurodegeneration. To study the pathophysiology of MGRN1 loss, we compared Mgrn1-knockout melanocytes with genetically matched controls and melan-md1 (mahoganoid) melanocytes. MGRN1 knockout induced a more differentiated and adherent phenotype, decreased motility, increased the percentage of cells in the S phase of the cell cycle and promoted genomic instability, as shown by stronger γH2AX labelling, increased burden of DNA breaks and higher abundance of aneuploid cells. Lack of MGRN1 expression decreased the ability of melanocytes to cope with DNA breaks generated by oxidizing agents or hydroxyurea-induced replicative stress, suggesting a contribution of genomic instability to the mahoganoid phenotype. MGRN1 knockout in B16-F10 melanoma cells also augmented pigmentation, increased cell adhesion to collagen, impaired 2D and 3D motility and caused genomic instability. Tumors formed by Mgrn1-KO B16-F10 cells had lower mitotic indices, fewer Ki67-positive cells and showed a trend towards smaller size. In short-term lung colonization assays Mgrn1-KO cells showed impaired colonization potential. Moreover, lower expression of MGRN1 is significantly associated with better survival of human melanoma patients. Therefore, MGRN1 might be an important phenotypic determinant of melanoma cells

    Volatile compounds and phenolic composition of skins and seeds of 'Cabernet Sauvignon' grapes under different deficit irrigation regimes

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    [EN] Aroma compounds and skin and seed pohphenols arc determinants of wine composition. The aim of this study was to determine the effect of different post-veraison deficit irrigation strategies on volatile profile and the chemical composition of grape skin and seeds in a 'Cabernet Sauvignon' vineyard in Valencia (Spain). Besides a non-irrigated regime (rainfed), irrigation treatments consisted of replacing 25, 50 and 75 % of the estimated crop evapotranspiration (ETC). When compared to rainfed vines, watering during post-veraison at 75 % of the ETC, decreased concentrations of alcohols but increased those of aldehydes such as hexanal, related to herbaceous (non-desirable) aromas in wines. Irrigating at 25 % or 50 % of ETC resulted in similar concentrations of grape volatile compounds than rainfed vines. There was also a general trend in a reduction in skin to flesh ratio as irrigation regime increased. The concentration of skin anthocyanins and tannins increased with water applications, but seed tannins decreased in the most irrigated regimes. This suggests different effects of water stress on skin and seed polyphenol synthesis and accumulation. For the tannin content, water stress provoked higher tannin mean degree polymerization values, which positively affect must astringency. Under the experimental conditions of the present study, watering at 50 % ETC during post-veraison is the recommended irrigation strategy for optimizing grape composition and improving yield in comparison with rainfed vines.This research was funded by the Spanish Ministry of Economy, Industry and Competitiveness MINECO (FEDER co-financing Projects TRACE 2009_00120 and AGL2014-54201-C4-4-R) and a grant agreement with Celler del Roure Winery.García Esparza, MJ.; Abrisqueta, I.; Escriche Roberto, MI.; Intrigliolo, D.; Alvarez Cano, MI.; Lizama Abad, V. (2018). Volatile compounds and phenolic composition of skins and seeds of 'Cabernet Sauvignon' grapes under different deficit irrigation regimes. VITIS. 57(3):83-91. https://doi.org/10.5073/vitis.2018.57.83-91S839157

    Short-Term Environmental Enrichment Rescues Adult Neurogenesis and Memory Deficits in APPSw,Ind Transgenic Mice

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    Epidemiological studies indicate that intellectual activity prevents or delays the onset of Alzheimer's disease (AD). Similarly, cognitive stimulation using environmental enrichment (EE), which increases adult neurogenesis and functional integration of newborn neurons into neural circuits of the hippocampus, protects against memory decline in transgenic mouse models of AD, but the mechanisms involved are poorly understood. To study the therapeutic benefits of cognitive stimulation in AD we examined the effects of EE in hippocampal neurogenesis and memory in a transgenic mouse model of AD expressing the human mutant β-amyloid (Aβ) precursor protein (APPSw,Ind). By using molecular markers of new generated neurons (bromodeoxiuridine, NeuN and doublecortin), we found reduced neurogenesis and decreased dendritic length and projections of doublecortin-expressing cells of the dentate gyrus in young APPSw,Ind transgenic mice. Moreover, we detected a lower number of mature neurons (NeuN positive) in the granular cell layer and a reduced volume of the dentate gyrus that could be due to a sustained decrease in the incorporation of new generated neurons. We found that short-term EE for 7 weeks efficiently ameliorates early hippocampal-dependent spatial learning and memory deficits in APPSw,Ind transgenic mice. The cognitive benefits of enrichment in APPSw,Ind transgenic mice were associated with increased number, dendritic length and projections to the CA3 region of the most mature adult newborn neurons. By contrast, Aβ levels and the total number of neurons in the dentate gyrus were unchanged by EE in APPSw,Ind mice. These results suggest that promoting the survival and maturation of adult generated newborn neurons in the hippocampus may contribute to cognitive benefits in AD mouse models

    Respuesta del melocotonero extra-temprano al riego deficitario

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    The effect of different irrigation strategies in water relations, vegetative growth and yield of early maturing peach trees, growing in Murcia, (Spain) was studied during two years. Treatments consisted on: a control T1, full irrigated (150% of ETc); T2, continuous deficit irrigation at 50% of ETc; T3, regulated deficit irrigation (RDI), irrigated at 100% of ETc only during stage III of fruit growth and 25% the rest of the growing season; and T4, with automatic control of irrigation based on capacitance FDR-type probe data, varying threshold values. The results indicated that irrigation deficits in T2 and T3 treatments induced the lowest soil water content and stem water potential(Ψstem) values during the postharvest period (e.g. ψstem up to –1.8 MPa in T3 during summer 2008); thus, a reduction in trunk growth and pruning weight, respect to control treatment values, was noted in both years. Also, peach yield was significantly reduced in both deficit irrigated treatments. The greatest irrigation water saving in T3 treatment (≈60%) caused the higher water use efficiency values in this treatment. For these reasons, water deficit during the postharvest periods (extended in the early maturing varieties) must be limited if fruit yield is not to be reduced. Irrigation scheduling based on capacitance probes have become a useful tool in the control of soil water content. When threshold values were precisely defined, the slight water deficits limited only vegetative growth while maintaining similar peach yield to that of well irrigated trees.Se estudió el efecto de distintas estrategias de riego deficitario en las relaciones hídricas, crecimiento vegetativo y producción de melocotoneros extratempranos ‘Flordastar’ en Murcia (España) durante dos años. Los tratamientos fueron: T1, control, riego diario al 150% ETc; T2, riego deficitario continuo (50% ETc); T3, riego deficitario controlado (100% ETc sólo durante la fase III de crecimiento del fruto y 25% ETc el resto del ciclo) y T4, control automático del riego basado diferentes umbrales de contenido hídrico del suelo con sondas de capacitancia. El déficit hídrico en los tratamientos T2 y T3 indujo los valores más bajos de contenido de agua del suelo y de potencial hídrico de tallo ( Ψtallo) durante la poscosecha (Ψtallo = –1,8 MPa en T3, verano 2008); lo que provocó una reducción del crecimiento del tronco y peso de poda, respecto a los valores del tratamiento control, en los dos años. Además, la producción se redujo significativamente en ambos tratamientos. El tratamiento T3 resultó ser el más eficiente en el uso del agua debido al mayor ahorro de agua de riego (≈60%). Por estas razones, los déficits hídricos en poscosecha (muy larga en variedades tempranas) deben ser limitados si no se quiere afectar la producción. Las sondas de capacitancia son una herramienta útil para el control del contenido hídrico del suelo, permitiendo una programación eficiente del riego. Cuando los valores umbrales se definen con precisión, se generan déficits hídricos ligeros que limitan sólo el crecimiento vegetativo manteniendo la producción similar a la de árboles bien regados

    Soil water balance trial involving capacitance and neutron probe measurements

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    The objective of this study was to compare soil water measurements made using capacitance and neutron probes by means of a water balance experiment in a drainage lysimeter. The experiment was conducted in a 5-year-old drip-irrigated peach orchard (Prunus persica L. Batsch, cv. Flordastar, on GF-677 peach rootstock) planted in a clay loam textured soil located in southern Spain. Four drainage lysimeters (5mx5mx1.5m), each containing one tree, were constructed and equipped with one lateral line containing eight drippers per tree, with a discharge rate of 2Lh-1. Three access tubes for the neutron probe (NP), symmetrically facing three PVC access tubes containing the multi-depth capacitance probes (MDCP) were located perpendicularly to the drip line (0.2, 0.6 and 1m). The results demonstrated that both the capacitance and neutron probes gave similar soil water content values under steady state hydraulic gradient conditions (0.2m from the emitter) although some discrepancies were found in heterogeneous soil water distribution conditions (1m from the emitter), which might be attributed to the smaller soil volume explored by the MDCP compared with the NP. Explanations for the discrepancies between both devised are presented. When water inputs and outputs were fairly constant, the volumetric soil water content could be considered to represent field saturation ([theta]sat=0.36m3m-3). When drainage was zero, there were 2 days when the soil water content was constant and could be considered as field capacity ([theta]fc=0.31m3m-3). The findings suggest that: (i) capacitance probes can be used for continuous real-time soil water content monitoring unlike the manual measurements obtained with the neutron probe; (ii) the location of the sensors is critical when used for drip irrigation scheduling and our recommendations for practical agricultural purposes would be to place MDCP sensors in the place representing the highest root density, leading the sensors to become biological sensors rather than mere soil moisture sensors; and (iii) on average, the water balance values determined by lysimeter match those calculated using the data from both probes. However, due to the smaller soil volume explored by MDCP, more of these sensors must be used to characterize the soil water status in water balance studies.Soil water content Capacitance probe Neutron probe Drainage lysimeter Water balance Drip irrigation
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