Effects of hyperbaric oxygen treatment on implant osseointegration in experimental diabetes mellitus

Objective: To evaluate whether hyperbaric oxygen (HBO) treatment has a favorable effect on implant osseointegration in diabetic rabbits. Material and Methods: An experimental diabetes model was induced in 32 New Zealand rabbits through IV injection of alloxan. After the state of diabetes had been confirmed, one dental implant was placed in the metaphysical region of each animal’s tibia. After the implants’ placements, the animals were divided into two groups. Half of the animals underwent HBO treatment, while the other group did not receive HBO treatment and served as the control group. The animals were euthanized at the 4th and 8th weeks. The osseointegration of the implants were compared by histomorphometry and resonance frequency analysis (RFA). Results: The Bone Implant Contact (BIC) values were significantly higher in the HBO group than in the control group at the 4th week. There was no difference in the BIC values between the groups at the 8th week. There was no significant difference in the RFA scores between the groups both at the 4th and 8th weeks after the operation. Conclusion: Histomorphometry findings suggest that HBO has positive effect on implant osseointegration in the early healing period in diabetic rabbits. However, implant stability is not affected by HBO treatment

Warburg Micro Syndrome 1 due to Segmental Paternal Uniparental Isodisomy of Chromosome 2 Detected by Whole-Exome Sequencing and Homozygosity Mapping

Warburg micro syndrome (WARBM) is a rare autosomal recessive disorder characterized by microcephaly, cortical dysplasia, intellectual disability, ocular abnormalities, spastic diplegia, and microgenitalia. WARBM has 4 subtypes arising from pathogenic variants in 4 genes (RAB18,RAB3GAP1,RAB3GAP2, andTBC1D20). Here, we report on a patient with a homozygous pathogenic c.665delC (p.Pro222HisfsTer30) variant in theRAB3GAP1gene identified by whole-exome sequencing (WES) analyses. Only his father was a heterozygous carrier, and homozygosity mapping analysis of the WES data revealed large loss-of-heterozygosity regions in both arms of chromosome 2, interpreted as uniparental isodisomy. This uniparental disomy pattern could be due to paternal meiosis I nondisjunction because of the preserved heterozygosity in the pericentromeric region. This report provides novel insights, including a rare form of UPD, usage of homozygosity mapping analysis for the evaluation of isodisomy, and the first reported case of WARBM1 as a result of uniparental isodisomy

Atropine sulphate test can be an aetiologic indicator of vagal bradycardia developed in a megaoesophagus case

An acquired megaoesophagus in a calf has been described. The diagnosis of megaoesophagus was made based on clinical and laboratory examinations, using contrast radiography, and necropsy findings. Atropine sulphate was used to enlighten the relationship between damage in the N. vagus and bradycardia, which developed in the megaoesophagus case

Hydrophobic Pesticide Endosulfan (alpha plus beta) and Endrin Sorption on Different Types of Microplastics

Ease of plastics production has caused high usage in many areas from water bottles, furniture and packaging to cosmetics during the last decade. Plastic products cause environmental pollution due to the high demand in the industry. It is known that pesticides are used to prevent various pests in order to increase production efficiency. With the use of such substances, there is considerable growth in the amount of product. However, pesticides remain on the water, soil, fruits, and vegetables for a long time, causing environmental pollution and thus leading to various damages that can reach people through the food chain. Since some of the mostly found pesticides in the environment are endosulfan and endrin, the sorbing capacities of endosulfan and endrin on microplastics have been investigated. The kinetic parameters at 23 degrees C in ultrapure water, hexane and saline water have been tested to show the effect of solvents on sorption behaviors

Rupture of the gastrocnemius muscle in a cow two months after twin birth

The rupture of the gastrocnemius muscle was described in a cow who gave a twin birth. A tentative diagnosis of complete rupture of the muscle was based on information obtained from both anamnesis and clinical signs. The definitive diagnosis was made on necropsy. The role of serum minerals, especially Ca, changes in the muscle originated enzymes, and other laboratory parameters together with clinical examination findings was discussed

Effects of hyperbaric oxygen treatment on implant osseointegration in experimental diabetes mellitus

Abstract Objective To evaluate whether hyperbaric oxygen (HBO) treatment has a favorable effect on implant osseointegration in diabetic rabbits. Material and Methods An experimental diabetes model was induced in 32 New Zealand rabbits through IV injection of alloxan. After the state of diabetes had been confirmed, one dental implant was placed in the metaphysical region of each animal’s tibia. After the implants’ placements, the animals were divided into two groups. Half of the animals underwent HBO treatment, while the other group did not receive HBO treatment and served as the control group. The animals were euthanized at the 4th and 8th weeks. The osseointegration of the implants were compared by histomorphometry and resonance frequency analysis (RFA). Results The Bone Implant Contact (BIC) values were significantly higher in the HBO group than in the control group at the 4th week. There was no difference in the BIC values between the groups at the 8th week. There was no significant difference in the RFA scores between the groups both at the 4th and 8th weeks after the operation. Conclusion Histomorphometry findings suggest that HBO has positive effect on implant osseointegration in the early healing period in diabetic rabbits. However, implant stability is not affected by HBO treatment

Effect of Gum Chewing and Coffee Consumption on Intestinal Motility in Caesarean Sections

Akpak, Yasam Kemal/0000-0002-1699-8667WOS: 000423935700012Aim: The time to first postoperative flatulence and defaecation are two of the main factors that determine caesarean patient comfort and morbidity. In this study, the effect of postoperative gum chewing and decaffeinated coffee consumption on intestinal motility was tested in full-term caesarean sections without complication with a prospective, randomised controlled trial. Material and Method: 100 women who underwent caesarean section without a complication were divided into 4 groups using the simple randomisation method: Group 1 (women given sugar-free gum at 4-hour intervals after postoperative hour 2 until defaecation) n:25; Group 2 (women given 100 mL coffee at 4-hour intervals beginning from postoperative hour 2 until defaecation for three times a day) n:25; Group 3 (Sham group-women given 100 ml hot water at 4-hour intervals beginning from postoperative hour 2 until defaecation for three times a day) n:25; and Group 4 (control group) n:25. Time to sensation of first bowel movement and time to passage of first flatus and defaecation were compared between the groups. Results: Times to sensation of first bowel movement and passage of first flatus were significantly shorter in Group 1 compared to the sham and control groups. Time to first defaecation was also detected to be significantly shorter in Groups 1, 2, and 3 compared to the control group. No significant difference could be detected between the groups. Discussion: Sugar-free gum chewing and decaffeinated coffee consumption during postoperative period after caesarean section may decrease the time to first flatulence and defaecation and thus, the hospitalisation time

Serum Potassium-Lowering Effects of Insulin Plus Dextrose and Adrenalin Treatment that Enhance Intracellular Potassium Transitions in Hyperkalemic Diarrheic Calves

The study examined the serum potassium-lowering effects of different treatment options for hyperkalemia in diarrheic calves (n=18). Calves were allocated into three treatment groups. All groups received isotonic sodium bicarbonate solution for a period of 90 minutes. The animals in group 1 received no further treatment, those in group 2 received a further dose of 50% dextrose and insulin, and those in group 3 received a further dose of adrenaline. High serum K levels in all groups significantly decreased after treatment (AT) than before treatment (BT). Serum potassium levels AT in groups 2 and 3 were found to be significantly lower than those in group 1. Increase in pH, bicarbonate and total carbon dioxide values were observed AT in all groups. However, bicarbonate and total carbon dioxide levels in the AT were not statistically significantly different from those in the adrenaline group compared with the groups 1. These parameters were significantly lower in the group 2. Moreover, the concentration of glucose in the groups 2 in contrast to the adrenalin group was higher than that in both the BT and group 1 AT. The insulin + dextrose combination and adrenaline applications in hyperkalemic calves had similar effects. However, the administration of adrenaline had a lower negative effect on metabolic acidosis treatment. (C) 2015 PVJ. All rights reserve

The Effect of Oxfendazole plus Oxyclozanide Paste and Tablet Formulations on Parasite Burden and Metabolic Status of Sheep

The aim of the present study was to evaluate the effects of anthelmintic treatment with Oxyclozanid + Oxfendazole in different formulation (paste and tablet) on parasite load and metabolic status in sheep. Forty five sheep, infected with various gastrointestinal parasites and kept on a grazing flock, were evenly divided into 3 groups. Sheep in the control group did not receive any treatment. The sheep in the remaining groups received the same dose of Oxyclozanid (5 mg kg(-1) body weight) and Oxfendazole (13.5 mg kg(-1) body weight) in combination either as tablet or as paste. Faeces and blood samples were collected pre-treatment (day 0) and on day 14 post-treatment. Several gastrointestinal nematode parasites were detected in the faecal samples. Eggs PerGram (EPG) on days 0 and 14 were 100 +/- 18 and 78 +/- 8 in CG, 96 +/- 12 and 6 +/- 3 in TG and 76 +/- 9 and 2 +/- 2 in the PG, respectively. Serum total protein and globulin concentrations increased significantly (p<0.05) in CG and PG on day 14 compare to pre-treatment levels. In both sampling times total protein levels did not differ whereas a slight decrease in paste group and a significant decrease in tablet group were detected concerning globulin level, but no significant difference was detected between the tablet group and paste group on day 14 post-treatment. In paste group serum albumin level increased (p<0.05) on day 14 compare to baseline level. Compare to control group, albumin level slightly increased in tablet group, whereas it significantly increased in paste group on day 14 post-treatment. On day 14 in paste group Pi, Mg and Cu levels were higher (p<0.05) than the post-treatment levels of the remaining groups. A combination of Oxyclozanid+Oxfendazole is effective to eliminate parasite burden in sheep. Drug formulation does not have any influence on the metabolic status of the sheep

Relationship between Packed Cell Volume Levels and Serum Nitric Oxide Concentrations in Cattle with Tropical Theileriosis

This study was performed to determine changes in serum NO (nitric oxide) levels in cattle with theileriosis in accordance with PCV levels. For this purpose, 42 cattle with theileriosis and 10 healthy cattle have been used as material. Infected animals have been divided into 3 equal groups. 1st group had <12%, 2nd group had 13-24% and 3rd group had <25% PCV level. Blood samples have been used to determine NO concentrations. Serum NO concentrations prior to treatment increased in all infected groups compared to control group however its levels were significant only in the third group. NO concentrations decreased to almost normal values after treatment especially in 2nd and 3rd group, however this decrease was not significant in the first group. As a result, NO levels have been found increased in cattle with natural theileriosis. Therefore, its concluded that NO level and PCV levels in theileriosis should be studied with more detail in the future to enlighten the role of NO release and strategies for controlling its' release should be made especially in severe cases. (C)2015 PVJ. All rights reserve