Bacterial co-infections in a captive Python bivittatus with septicemia

This case reports bacterial co-infection in a dead albino python (Python bivittatus). The snake was brought in dead to the Universiti Veterinary Hospital, Universiti Putra Malaysia. Necropsy was conducted and organ samples were sent for virus and bacterial isolation, as well as histopathology. Gross pathological lesions include congestion of the heart, spleen and liver and presence of superficial whitish circumscribed spots in the lungs. Bacterial culture showed the presence of Salmonella enterica ssp arizona, Plesiomonas shigelloides and Enterobacter cloacae. Histopathological evaluation showed evidence of necrosis, degeneration and heterophil infiltration in the liver, kidney, heart and lungs. The snake was diagnosed to have died of bacterial septicemia.Keywords: Bacterial isolation, Captive snake, Histopathology, Python bivittatus, Septicemi

Exfoliative cytology of the uterus and vagina during the follicular and luteal phases in one-humped camel (Camelus dromedarius)

The exfoliative cytology of the uterus and vagina area was evaluated in one-humped camel during the follicular and luteal phases. This was carried out using intact genitalia collected from camels immediately after slaughter. A total of 86 genitalia and blood samples were collected and processed. The mean serum progesterone (P4) profile was determined and used to categorize the animals into the follicular (n=51) and luteal phases (n=35). The result of the differential cell counts of the exfoliated vaginal epithelial cells showed mean basal, superficial cells and leucocytes of 27.0%, 25.4% and 14%, respectively being the most prominent during the follicular phase, while the uterine smears were characterized by mean basal (25.6%) , intermediate cells (24.6%) and leucocytes (9.6%) respectively. During the luteal phase, the vaginal smears were characterized majorly by the intermediate (27.4%), parabasal cells (26.6%) and leucocytes (11.6%, while the uterine smears were predominantly characterized by intermediate (22.8%), superficial cells (22.6%) and leucocytes (17.8%) respectively. The result showed that the exfoliative cytology of the uterus and vagina are similar during both the follicular and luteal phases in camels, and so cannot be used alone to characterize these reproductive period. However, this finding has a significant potential application in the use of vaginal cytology alone to assess uterine cellular changes without necessarily invading the uterus under field conditions in camel.Keywords: Camels, Exfoliative cytology, Follicular, Utero-vagina, Luteal, Progesteron

Testicular and Related Size Evaluations in Nigerian Sahel Goats with Optimal Cauda Epididymal Sperm Reserve

Testicular sizes of animals are important for identification of those with adequate sperm production. The aim of this study was to define the testicular and related size estimates that would be associated with optimal cauda epididymal sperm counts (ESC) in Sahel goats based on postmortem evaluations. A stratified quota sample population of 125 male goats inclusive of all testicular sizes was taken at a slaughterhouse in Maiduguri, Nigeria. The bucks were aged 18–30 months and weighed 17.04 ± 2.99 (12–25) kg. Body, testicular, and epididymal weights of each goat with other related size measurements were estimated. ESC was determined from homogenized tissue using a manual cytometer. At the cut-off ESC of >1.1 × 109 sperm heads, 66 (52.80%) of the goats had optimal ESC which was associated with testicular weight of 59.90 ± 16.10 (31.40–86.20) g, gonadosomatic index of 3.51 ± 0.69 (2.00–4.50) g/kg, and scrotal circumference of 19.07 ± 1.29 (17.00–21.80) cm. The size variables of the scrotum and testis correlated with one another and with the ESC. These findings provide data that may be used to anticipate adequate antemortem sperm reserve based on testicular size during preliminary selection of sires for breeding from a sexually mature Sahel buck population

Volumineux Mélanome Malin De La Conjonctive Multi Récidivant À Propos d’un Cas

Melanoma of the conjunctiva is a tumor that develops from the conjunctival melenocytes. It often reoccurs even after several surgical removals. The authors present the case of a 45 years old woman who was at first seen in our outpatient clinic with a spitz naevus of the conjunctiva of the right eye since more than 20 years ago. This naevus which was excised twice and then the eye enucleated underwent malignant transformation into a voluminous multi-recidivant malignant melanoma weighing after surgical removal 350g and measures 6x12x5cm

Bacterial co-infections in a captive Python bivittatus with septicemia

This case reports bacterial co-infection in a dead albino python (Python bivittatus). The snake was brought in dead to the Universiti Veterinary Hospital, Universiti Putra Malaysia. Necropsy was conducted and organ samples were sent for virus and bacterial isolation, as well as histopathology. Gross pathological lesions include congestion of the heart, spleen and liver and presence of superficial whitish circumscribed spots in the lungs. Bacterial culture showed the presence of Salmonella enterica ssp arizona, Plesiomonas shigelloides and Enterobacter cloacae. Histopathological evaluation showed evidence of necrosis, degeneration and heterophil infiltration in the liver, kidney, heart and lungs. The snake was diagnosed to have died of bacterial septicemia

Assessment of the Relationship between Digit Lengths and Circumferences of the Waist and Hip Amongst Ugandans

Abstract: In human hand the second Digit (2D) and the fourth Digit (4D) present a pattern of approximate symmetry around the central axis of the third digit. Many individuals have longer 2D than 4D (2D>1) and many have longer 4D than 2D 2D:4D<1). The former ratio is more common in females and the later ratio is more in males. The 2D:4D ratio is fixed at an early age. There is evidence that 2D:4D is negatively associated with prenatal testosterone and positively with prenatal estrogen and that this ratio differs between male and female and different ethnic groups, with low values found in Black populations. This study sought to assess the relationship between sexually dimorphic traits established early in life and those established at puberty amongst Ugandans in Ishaka Bushenyi. One sixty nine males and one fifty four females Ugandans in Bushenyi district were used for this study. Anthropometric tape was used to measure waist and hip circumferences, a digital venier caliper was used to measure the lengths of the digit. Using Pearson's correlation, the lengths of the right index or second finger in females was found to have a positive correlation with the hip circumference while the length of the ring or fourth finger in the males was found to have a positive correlation with the waist circumference. This suggests that the lengths of the digit established early in life are positively correlated with the increased waist circumference in males and increased hip circumference in females that occur at puberty. Implying that the sexually dimorphic traits that appear in life during puberty under the influence of testosterone and estrogen is positively correlated with the sexually dimorphic traits that appear early in life due to prenatal exposure to the androgen

A molecular portrait of high-grade ductal carcinoma in situ

Ductal carcinoma in situ (DCIS) is a noninvasive precursor lesion to invasive breast carcinoma. We still have no understanding on why only some DCIS lesions evolve to invasive cancer whereas others appear not to do so during the life span of the patient. Here, we performed full exome (tumor vs. matching normal), transcriptome, and methylome analysis of 30 pure high-grade DCIS (HG-DCIS) and 10 normal breast epithelial samples. Sixty-two percent of HG-DCIS cases displayed mutations affecting cancer driver genes or potential drivers. Mutations were observed affecting PIK3CA (21% of cases), TP53 (17%), GATA3 (7%), MLL3 (7%) and single cases of mutations affecting CDH1, MAP2K4, TBX3, NF1, ATM, and ARID1A. Significantly, 83% of lesions displayed numerous large chromosomal copy number alterations, suggesting they might precede selection of cancer driver mutations. Integrated pathway-based modeling analysis of RNA-seq data allowed us to identify two DCIS subgroups (DCIS-C1 and DCIS-C2) based on their tumor-intrinsic subtypes, proliferative, immune scores, and in the activity of specific signaling pathways. The more aggressive DCIS-C1 (highly proliferative, basal-like, or ERBB2 + ) displayed signatures characteristic of activated Treg cells (CD4 + /CD25 + /FOXP3 + ) and CTLA4 + /CD86 + complexes indicative of a tumor-associated immunosuppressive phenotype. Strikingly, all lesions showed evidence of TP53 pathway inactivation. Similarly, ncRNA and methylation profiles reproduce changes observed postinvasion. Among the most significant findings, we observed upregulation of lncRNA HOTAIR in DCIS-C1 lesions and hypermethylation of HOXA5 and SOX genes. We conclude that most HG-DCIS lesions, in spite of representing a preinvasive stage of tumor progression, displayed molecular profiles indistinguishable from invasive breast cancer.Centro de Investigaciones Inmunológicas Básicas y Aplicada

A molecular portrait of high-grade ductal carcinoma in situ

Ductal carcinoma in situ (DCIS) is a noninvasive precursor lesion to invasive breast carcinoma. We still have no understanding on why only some DCIS lesions evolve to invasive cancer whereas others appear not to do so during the life span of the patient. Here, we performed full exome (tumor vs. matching normal), transcriptome, and methylome analysis of 30 pure high-grade DCIS (HG-DCIS) and 10 normal breast epithelial samples. Sixty-two percent of HG-DCIS cases displayed mutations affecting cancer driver genes or potential drivers. Mutations were observed affecting PIK3CA (21% of cases), TP53 (17%), GATA3 (7%), MLL3 (7%) and single cases of mutations affecting CDH1, MAP2K4, TBX3, NF1, ATM, and ARID1A. Significantly, 83% of lesions displayed numerous large chromosomal copy number alterations, suggesting they might precede selection of cancer driver mutations. Integrated pathway-based modeling analysis of RNA-seq data allowed us to identify two DCIS subgroups (DCIS-C1 and DCIS-C2) based on their tumor-intrinsic subtypes, proliferative, immune scores, and in the activity of specific signaling pathways. The more aggressive DCIS-C1 (highly proliferative, basal-like, or ERBB2 + ) displayed signatures characteristic of activated Treg cells (CD4 + /CD25 + /FOXP3 + ) and CTLA4 + /CD86 + complexes indicative of a tumor-associated immunosuppressive phenotype. Strikingly, all lesions showed evidence of TP53 pathway inactivation. Similarly, ncRNA and methylation profiles reproduce changes observed postinvasion. Among the most significant findings, we observed upregulation of lncRNA HOTAIR in DCIS-C1 lesions and hypermethylation of HOXA5 and SOX genes. We conclude that most HG-DCIS lesions, in spite of representing a preinvasive stage of tumor progression, displayed molecular profiles indistinguishable from invasive breast cancer.Centro de Investigaciones Inmunológicas Básicas y Aplicada

Clinical and histopathological study on reproductive lesions caused by Pasteurella multocida type B2 immunogens in buffalo heifers

Haemorrhagic septicaemia (HS) is a globally renowned disease that affects cattle and buffaloes. Its effects on the reproductive system have not been previously studied. The present study evaluated the pathological responses in pre-pubertal female buffaloes infected with immunogens; lipopolysaccharide (LPS) and outer membrane protein (OMP) derived from P. multocida type B:2. Fifteen healthy pre-pubertal female buffaloes were divided into 5 equal groups. Buffaloes in groups 1 and 2 were intravenously and orally inoculated with P. multocida LPS, while those in groups 3 and 4 were sub-cutaneously and orally inoculated with P. multocida OMP. Buffaloes in group 5 were inoculated with sterile phosphate buffered saline (negative control). During the post infection period, all buffaloes were examined for clinical signs throughout 21 days and surviving animals were euthanised for postmortem evaluation. Histopathological evaluation of buffaloes inoculated intravenously with LPS showed a significantly higher occurrence (P<0.05) of necrosis and degeneration in the uterine horn and supramammary glands . Following inoculation with OMP, haemorrhage and congestion was significantly higher (P<0.05) in the ovaries, cervix, vagina, mammary and supra-mammary glands of buffaloes inoculated subcutaneously, while necrosis and degeneration and inflammatory cell infiltration in different organs was also significantly increased in the same group. The findings showed that both P. multocida bacterial LPS and OMP affected the uterine horn, ovaries, cervix, mammary gland and supramammary lymph nodes of buffalo calves inoculated either intravenously or subcutaneously, as compared to those inoculated orally

Clinical management of dietary induced urolithiasis associated with balanoposthitis in a Boer goat

A Boer-Kajang cross male goat was presented to the Veterinary Hospital, University Malaysia Kelantan with a history of dysuria, hematuria and restlessness. The goat was intensively managed (confined to the pen) and fed with only palm kernel cake for the last three months. Physical examination revealed that the goat was dull, depressed, having an inflamed penis and prepuce with blood stained urine dripping from the penis. The differential diagnoses were obstructive urolithiasis, urinary tract infection and balanoposthitis. Based on the history, clinical signs, physical examination, urinalysis, ultrasonagraphy and feed analysis, the goat was diagnosed with obstructive urolithiasis and balanoposthitis. Treatment was instituted by amputation of the urethral process and retrograde urohydropulsion to relieve the blockade. Sulfadiazine-trimethoprim (Norodine®24) 15mg/kg, I.M; flunixin meglumine 2.2mg/kg, I.M; vitamin B complex 1ml/10kg, I.M and ammonium chloride 300mg/kg orally were administered. The goat responded well to treatment and was recovering well during a follow up visit