Anti-tuberculosis activity of -lactam antibiotics: prospects for the treatment of multi-drug-resistant tuberculosis

Tuberculosis (TB) is a global problem and the spread of multi-drug-resistant (MDR) TB, defined as resistance to at least isoniazid and rifampicin, is a formidable challenge for control programs. There are no widely accepted standard regimens for the treatment of MDR-TB. However, a considerable proportion of cases can be cured with a regimen containing both first-line and second-line drugs. In order to prevent the development of resistance to drugs already in use and also to try to develop a standard regimen, there is need to look for new drugs. This review is prepared to show results on the anti-TB activity of -lactam antibiotics. -Lactams are among the oldest drugs with little or no side effects. Both in vitro studies and clinical data indicate that -lactams have a promising activity for use in the management of MDR-TB. More studies are required to define the interaction of -lactams with other first-line and second-line drugs, and to clearly show the clinical usefulness in the management of MDR-TB. (Ethiopian Journal of Health Development, 2000, 14(3): 269-276

Determinants of Stunting among Children Aged 6-23 Months of Age in Pastoral Community, Afar Region, Ethiopia: Unmatched Case-Control Study

Background: Globally, stunting is a public health concern, more of in developing counties, including Ethiopia. Once occurred, in the first two years of life, it is irreversible and has long-lasting harmful consequences. Exploring the determinants has pivotal importance for evidence-based interventions. Therefore, the rationale of this study was to identify determinants of stunting among children aged 6-23 months in the pastoralist community, Afar region, Ethiopia.Method: A community-based unmatched case-control study was conducted among 381 (cases=126, controls 255) study participants from February 15/2017 to March 30/2017. Cases and controls were identified consecutively using the world health organization growth monitoring chart.Data was collected by interviewer-administered questionnaires and anthropometric measurements. Statistical significance was declared at p-value < 0.05 in the final multivariable logistic regression model.Result: Maternal education (AOR:0.34, 95% CI: 0.16, 0.77), maternal under-nutrition (AOR:2.91, 95% CI:1.51, 5.60), number of under-five children within the household (AOR:2.66, 95% CI: 1.38, 5.10), latrine ownership (AOR:0.28, 95% CI:0.15, 0.55), minimum Dietary Diversity score of children (AOR:0.41, 95% CI:0.22, 0.75), child age (AOR:1.76, 95% CI:1.01, 3.09), colostrum intake (AOR:3.03, 95%CI:1.62, 5.66), and exclusively breastfeed for the first six months (AOR:3.20, 95% CI:1.72,5.95) were found to be determinants of stunting.Conclusion: This study found that determinants of childhood stunting are multifactorial. Maternal, household and child-related characteristics are associated with childhood stunting. Therefore, to improve childhood nutritional status, inter-sectoral collaboration and commitment are vital

Disease-Related Knowledge and Practices of Tuberculosis Patients

BACKGROUND: Tuberculosis (TB) is a major public health problem. World Health Organization (WHO) recommends the use of directly observed treatment, short course (DOTS) strategy for effective control of TB. Disease-related knowledge, beliefs and practices need to be recognized to tailor the DOTS strategy into the local norms. This study was performed with two objectives: i) to assess TB-related knowledge among TB patients in two rural hospitals in Ethiopia, ii) to assess the practices that contribute to delay in the initiation of anti-TB treatment. METHODS: The study was conducted between June 2000 and May 2001.Two hundred twenty two known adult TB patients attending TB clinics in Jimma and Hossana hospitals were interviewed on their knowledge, attitude and practice related to TB using pre-tested structured questionnaire. Patients belonged to 14 ethnic groups. RESULTS: Sixty percent (134/222) were female. The average family size of patients was 5.3. The number of illiterate female patients was greater than that of males (p = 0.002). Only 36% (80/222) of patients gave a correct response on the transmission of TB. Similarly, only 34 % (76/222) knew about symptoms indicative of TB. Hundred and fifty (67.5 %) patients sought medical help after experiencing TB indicative symptoms for more than one month. Age, sex, education, occupation, and religion did not affect TBrelated knowledge. Thirty-seven (16.7 %) patients used traditional medicine for at least one week before they came to a health institution. The diagnosis of TB had a negative social impact. Forty-eight (21.6 %) patients were either divorced, expelled from their family, abandoned by friends, or lost job. CONCLUSION: Health education targeting basic concepts on the transmission of TB and also targeting the need for early diagnosis is important in TB control. TB control programs may need to include traditional healers into the control strategy to refer patients with specific symptoms.Ethiop J Health Sci. Vol. 13, No. 1 January 72 200

Evaluation of a direct colorimetric assay for rapid detection of rifampicin resistant Mycobacterium tuberculosis

No Abstract Available Ethiop.J.Health Dev. Vol.19(1) 2005: 51-5

Manual for production of Chamo strain Nile tilapia fingerlings and preparation of fish feeds from locally available material

Climate change has resulted in increased local temperatures that in turn have increased evapotranspiration, and negatively affected the breeding grounds of several important fish species due to reduction in the water levels of water bodies (Ficke et al. 2007). The rise in local temperature also affects the physico-chemical properties of water, including temperature, pH, dissolved oxygen, salinity, and concentration of different ions in water bodies (El Morhit & Mouhir 2014). These changes negatively affect the physiological (e.g. reproduction) and behavioral dynamics of fish as well as their natural feed leading to lower production of capture fish (Chabot & Guénette 2013). To increase production of capture fish, there is need for a reliable method for mass production of fry. Although tilapia breed freely in ponds, it is important for farmers (producers) to consider using properly produced fingerlings. However, there are quality challenges with semi-natural or hormone-induced propagation of fingerlings. Quality fingerlings in tilapia aquaculture are needed for improved production. For this reason, it is advisable for farmers to generate their own fingerlings if they cannot ascertain the quality of those from other sources. Poor fingerlings result in poor harvests. Therefore, artificial propagation under controlled environmental conditions in a hatchery has become a necessity to ensure production en-masse of fry and fingerlings

BCG Vaccination Induces M. avium and M. abscessus Cross-Protective Immunity

Pulmonary non-tuberculous mycobacterial (NTM) infections particularly caused by Mycobacterium avium complex (MAC) and Mycobacterium abscessus (MAB) are becoming major health problems in the U.S. New therapies or vaccines which will help prevent the disease, shorten treatment duration and/or increase treatment success rates are urgently needed. This study was conducted with the objective of testing the hypothesis that Bacillus Calmette Guerin (BCG), a vaccine used for prevention of serious forms of tuberculosis (TB) in children and adolescents in tuberculosis hyperendemic countries, induces cross-protective T cell immunity against Mycobacterium avium (MAV) and MAB. Human TB and NTM cross-protective T cells were quantified using flow cytometric assays. The ability of BCG expanded T cells to inhibit the intracellular growth of MAV and MAB was assessed in co-cultures with infected autologous macrophages. In both BCG-vaccinated and M. tuberculosis (Mtb)-infected mice, NTM cross-reactive immunity was measured using IFN-γ ELISPOT assays. Our results demonstrate the following key findings: (i) peripheral blood mononuclear cells from TB skin test-positive individuals contain MAV and MAB cross-reactive T cells, (ii) both BCG vaccination and Mtb infection of mice induce MAV and MAB cross-reactive splenic cells, (iii) BCG-expanded T cells inhibit intracellular MAV and MAB, (iv) CD4, CD8, and γδ T cells play important roles in inhibition of intracellular MAV and MAB and (v) BCG vaccination of healthy volunteers induces TB and NTM cross-reactive T cells. In conclusion, BCG-vaccination induces NTM cross-reactive immunity, and has the potential for use as a vaccine or immunotherapy to prevent and/or treat pulmonary NTM disease

First PCR Confirmed anthrax outbreaks in Ethiopia-Amhara region, 2018-2019.

BackgroundAnthrax is a disease that affects humans and animals. In Ethiopia, anthrax is a reportable disease and assumed to be endemic, although laboratory confirmation has not been routinely performed until recently. We describe the findings from the investigation of two outbreaks in Amhara region.MethodsFollowing reports of suspected outbreaks in Wag Hamra zone (Outbreak 1) and South Gondar zone (Outbreak 2), multi-sectoral teams involving both animal and public health officials were deployed to investigate and establish control programs. A suspect case was defined as: sudden death with rapid bloating or bleeding from orifice(s) with unclotted blood (animals); and signs compatible with cutaneous, ingestion, or inhalation anthrax ≤7 days after exposure to a suspect animal (humans). Suspect human cases were interviewed using a standard questionnaire. Samples were collected from humans with suspected anthrax (Outbreak 1 and Outbreak 2) as well as dried meat of suspect animal cases (Outbreak 2). A case was confirmed if a positive test was returned using real-time polymerase chain reaction (qPCR).ResultsIn Outbreak 1, a total of 49 cows died due to suspected anthrax and 22 humans developed symptoms consistent with cutaneous anthrax (40% attack rate), two of whom died due to suspected ingestion anthrax. Three people were confirmed to have anthrax by qPCR. In Outbreak 2, anthrax was suspected to have caused the deaths of two livestock animals and one human. Subsequent investigation revealed 18 suspected cases of cutaneous anthrax in humans (27% attack rate). None of the 12 human samples collected tested positive, however, a swab taken from the dried meat of one animal case (goat) was positive by qPCR.ConclusionWe report the first qPCR-confirmed outbreaks of anthrax in Ethiopia. Both outbreaks were controlled through active case finding, carcass management, ring vaccination of livestock, training of health professionals and outreach with livestock owners. Human and animal health authorities should work together using a One Health approach to improve case reporting and vaccine coverage