Live Cell Imaging of Bone Marrow Stromal Cells on Nano-pitted and Polished Titanium Surfaces: A Micro-Incubator in vitro Approach

Current orthopedic implants are not conducive for optimal integration of the biomaterial with newly-formed tissue (osseointegration) inside a patient’s body. In this study, medical-rade Ti-6Al-4V was used as a substrate due to its biocompatibility and ability to facilitate cellular adhesion and proliferation. Live cell imaging was conducted on bone marrow stromal cells, genetically modified to express the green fluorescent protein (GFP), from the 24-96 hours growth period, with the first 24 hours of growth being held inside a lab-scale incubator. Periodic images were recorded on nanopitted anodized and polished Ti-6Al-4V substrates to study how substratestiffness influences adhesion and proliferation. Collected images were analyzed for mitosis, adhesion, and filopodia-stretchability using ImageJ, an image processing program. Images were enhanced in order to perform cell counts at 24, 48, 72, and 96 hours of growth. Continuous recordings were produced to account for the number of mitosis occurrences and cellular migration on each of the substrates. Based on the conducted experiments, it appears that polished Ti-6Al-4V has a higher cell adherence than “nanopitted” anodized surface and an improved rate of proliferation which may be because the cells once adhered on the nano-pitted surface have less ability to detach in-order to undergo mitosis.https://engagedscholarship.csuohio.edu/u_poster_2014/1004/thumbnail.jp

Comprehensive Screening of Eight Known Causative Genes in Congenital Hypothyroidism With Gland-in-Situ.

CONTEXT: Lower TSH screening cutoffs have doubled the ascertainment of congenital hypothyroidism (CH), particularly cases with a eutopically located gland-in-situ (GIS). Although mutations in known dyshormonogenesis genes or TSHR underlie some cases of CH with GIS, systematic screening of these eight genes has not previously been undertaken. OBJECTIVE: Our objective was to evaluate the contribution and molecular spectrum of mutations in eight known causative genes (TG, TPO, DUOX2, DUOXA2, SLC5A5, SLC26A4, IYD, and TSHR) in CH cases with GIS. Patients, Design, and Setting: We screened 49 CH cases with GIS from 34 ethnically diverse families, using next-generation sequencing. Pathogenicity of novel mutations was assessed in silico. PATIENTS, DESIGN, AND SETTING: We screened 49 CH cases with GIS from 34 ethnically diverse families, using next-generation sequencing. Pathogenicity of novel mutations was assessed in silico. RESULTS: Twenty-nine cases harbored likely disease-causing mutations. Monogenic defects (19 cases) most commonly involved TG (12), TPO (four), DUOX2 (two), and TSHR (one). Ten cases harbored triallelic (digenic) mutations: TG and TPO (one); SLC26A4 and TPO (three), and DUOX2 and TG (six cases). Novel variants overall included 15 TG, six TPO, and three DUOX2 mutations. Genetic basis was not ascertained in 20 patients, including 14 familial cases. CONCLUSIONS: The etiology of CH with GIS remains elusive, with only 59% attributable to mutations in TSHR or known dyshormonogenesis-associated genes in a cohort enriched for familial cases. Biallelic TG or TPO mutations most commonly underlie severe CH. Triallelic defects are frequent, mandating future segregation studies in larger kindreds to assess their contribution to variable phenotype. A high proportion (∼41%) of unsolved or ambiguous cases suggests novel genetic etiologies that remain to be elucidated.This study made use of data generated by the UK10K Project and we acknowledge the contribution of the UK10K Consortium. This work was supported by Wellcome Trust Grants 100585/Z/12/Z (to N.S.), and 095564/Z/11/Z (to V.K.C.) and the National Institute for Health Research Cambridge Biomedical Research Center (to V.K.C., N.S.). E.G.S and C.A.A. are supported by the Wellcome Trust (098051). Funding for the UK10K Project was provided by the Wellcome Trust under award WT091310

Birinci karpometakarpal eklem osteoartozunda trapeziektomi ve tendon interpozisyon/süspansiyon artroplastisi sonuçları

Birinci parmağın karpometakarpal (KMK) eklemi insan elinin kavrama konusundaki üstünlüğünü sağlamada çok önemli ve karakteristik bir fonksiyona sahip, eyer tipinde ve geniş hareket açıklığına sahip bir eklemdir. Başparmağın en tabanında yer alan bu eklem hayat boyu çok farklı fiziksel streslere maruz kalmaktadır. Osteoartroz, romatoid artrit, kapsüler ligamentöz yapıların postmenapozal laksitesi gibi durumlar bu önemli eklem yüzeyinin subluksasyonu ve ilerleyen dislokasyonuna neden olabilmekte, rotasyon aksının yer değiştirmesi ve kas kuvvetlerinin anormal etkilerinin ortaya çıkması ile artroz süreci geri dönülmez bir hal almaktadır. Birinci parmak KMK eklem osteoartrozuna günlük ortopedi kliniğinde çok sık rastlanır ve genellikle postmenapozal kadınlarda görülmesi ile öne çıkan bir patolojidir. Erkeklerde %7 insidansla bildirilmiş olan radyografik osteoartroz prevelansı postmenapozal kadın popülasyonunda %33'e yükselmektedir. Birinci karpometakarpal eklem osteoartrozu nedeniyle ortalama cerrahi uygulanma yaşı 60 olmakla birlikte erkeklere uygulanan cerrahi insidansı radyografik insidansa oranla daha da fazladır. Çalışmamızda bu hastalığın cerrahi tedavisinde uygulanan trapeziektomi ve tendon interpozisyon/süspansiyon artroplastisi operasyonunun sonuçları retrospektif bir olgu-kontrol çalışması ile değerlendirilmiş, değerlendirme genel veriler, hareket açıklığı ve kuvvet değerlendirmesi, ağrı ve memnuniyet skorlamaları üzerinden yapılmıştır. Sonuç olarak çalışmamızda bu operasyonun ağrılı KMK eklem osteoartrozunda doğru endikasyonlarla uygulandığında ağrının %82 oranında azaltılması, % 15,2 fleksiyon kaybı, %19,7 ekstansiyon kaybı, %6,9 abdüksiyon kaybı ile tatmin edici düzeyde hareket açıklığı korunması, farklı gruplarda analiz edildiğinde %71 -%105 oranında kuvvet korunması özellikleri ile son derece etkin ve geçerli bir tedavi yöntemi olduğu gösterilmiştir.The carpometacarpal (CMC) joint of the first finger which is sellar in shape that leads to a great range of motion, has a really important and characteristic function on human hand's dexterity of grip. This joint that is at the very base of the thumb is exposed to a variety of physical stresses life long. Conditions like osteoarthrosis, rheumatoid arthritis, postmenapausal laxity of the capsular ligamentous structures may cause subluxation and further dislocation of this important joint surfaces, by the change of the rotational arc's and appearence of abnormal affects of muscle forces, the arthrosis process becomes inevitable. First CMC joint osteoarthrosis is frequently encountered in daily orthopaedics clinic and this pathology comes forward being seen especially in postmenapausal women. The radiographic osteoarthrosis prevelance in postmenapausal women is as high as 33% while it's reported as 7% in men. Although the mean surgery age for first carpometacarpal osteoarthrosis is 60, the incidence of the surgery performed on men is more than the radiographic incidence. In this study, the results of the trapeziectomy and tendon interposition/suspension arthroplasty operation were evaluated retrospectively with a case control study, the evaluation consisting of general data, range of motion, grip strength evaluation, pain and satisfaction score analysis. As a result of this study, for its properties like 82 % decrease in pain, satisfactory range of motion preservation by 15,2% flexion loss, 19,7%extansion loss, 6,9% abduction loss and 71% to 105% maintanence of grip strength with analisation of different groups, this operation is found to be a highly effective and valid treatment method, when it is performed on true indications, in painful first CMC joint osteoarthrosis

The moderating role of employee's moral identity on the relationship between perceived organizational politics and deviant behaviors

Bu çalışmada örgütsel politika ile sapkın davranışlar arasındaki ilişkide, ahlaki kimliğin düzenleyici rolü analiz edilmiştir. Araştırmanın örneklemini Nevşehir ilinde faaliyet gösteren otel işletmelerindeki 389 çalışan oluşturmaktadır. Regresyon analizi sonuçlarına göre örgütsel politika ile sapkın davranışlar arasında pozitif yönlü ve anlamlı bir ilişki olduğu, ahlaki kimlik ile sapkın davranışlar arasında ise negatif yönlü ve anlamlı bir ilişki olduğu tespit edilmiştir. Diğer yandan örgütsel politika ile ahlaki kimlik arasında anlamlı bir ilişki olmadığı ortaya konulmuştur. Benzer şekilde örgütsel politika ile sapkın davranışlar arasında ahlaki kimliğin düzenleyici rolü oynamadığı tespit edilmiştir. Gelecekteki araştırmaya yönelik öneriler sunulmuştur.In this study, the moderating role of moral identity in the relationship between organizational politics and deviant behavior was analyzed. The sample of the research consists of 389 employees in hotel enterprises operating in Nevşehir province. According to the results of the study, it was determined that there was a positive and significant relationship between organizational politics and deviant behaviors, and a negative and significant relationship between moral identity and deviant behaviors. On the other hand, it has been revealed that there is no significant relationship between organizational politics and moral identity. Similarly, it was determined that moral identity did not play a moderating role between organizational politics and deviant behaviors. Recommendations for future reserarch are presented