Prevention of ulcer disease in goldfish by means of vaccination

A vaccine comprising cells of Aeromonas bestiarum grown in tryptic soy broth and atypical A. salmonicida cells produced in iron-limited and iron-supplemented media protected goldfish Carassius auratus when administered by immersion (dosage ≈ 5 × 107 cells/mL for 60 s) followed after 28 d by an oral booster (dosage = 5 × 107 cells/g of feed), which was fed for 7 d so that each fish received about 1 g of vaccine-containing feed. After challenge by intramuscular injection of a virulent culture of atypical A. salmonicida, the relative percent survival (RPS) was more than 90%. The approach was more successful than using a commercial furunculosis vaccine with or without supplementation with A. bestiarum or atypical A. salmonicida cells. Moreover, a smooth derivative of the virulent rough culture of atypical A. salmonicida was less effective as a vaccine candidate, yielding an RPS of only 65%. Low antibody titers of 1:39–1:396 were found in the vaccinated fish. The vaccinated fish had a significantly higher proportion of dead head kidney macrophages (10.9 ± 3.5%; P = 0.0149) than did the controls (6.8 ± 3.1%). However, differences in the number of erythrocytes and leukocytes, the level of phagocytic and lysozyme activities, and the proportion of lymphocytes, monocytes, and polymorphonuclear cells were not statistically significant between the two groups

Conditionality Contaminates Conservation: Structural Adjustment and Land Protection in Less-Developed Nations

The destruction that human beings cause the natural environment is so catastrophic that the current era has now been labeled the “Sixth Extinction.” Conservation and the preservation of species and ecosystems is a leading strategy in preventing biodiversity loss and preserving natural ecosystems. As threats to biodiversity mount, it is imperative that social scientists explore the macro-level processes that influence conservation areas, especially in poorer nations where the majority of biodiverse zones are located. This study explores the impact of structural adjustment policies on the ability of less-developed nations to designate land for conservation. We use ordinary least squares (OLS) regression to examine the influence of IMF conditionality on levels of terrestrial protected areas for 86 less-developed nations. The results confirm our hypothesis that nations undergoing IMF structural adjustment have a smaller percentage of land devoted to terrestrial protected areas than nations not undergoing structural adjustment. Neoliberal approaches that encourage privatization and deregulation ultimately impair less-developed nations’ abilities to make conservation a priority

Absorption of Energy at a Metallic Surface due to a Normal Electric Field

The effect of an oscillating electric field normal to a metallic surface may be described by an effective potential. This induced potential is calculated using semiclassical variants of the random phase approximation (RPA). Results are obtained for both ballistic and diffusive electron motion, and for two and three dimensional systems. The potential induced within the surface causes absorption of energy. The results are applied to the absorption of radiation by small metal spheres and discs. They improve upon an earlier treatment which used the Thomas-Fermi approximation for the effective potential.Comment: 19 pages (Plain TeX), 2 figures, 1 table (Postscript

Do Hadronic Charge Exchange Reactions Measure Electroweak L = 1 Strength?

An eikonal model has been used to assess the relationship between calculated strengths for first forbidden beta decay and calculated cross sections for (p,n) charge exchange reactions. It is found that these are proportional for strong transitions, suggesting that hadronic charge exchange reactions may be useful in determining the spin-dipole matrix elements for astrophysically interesting leptonic transitions.Comment: 14 pages, 5 figures, Submitted to Physical Review

Single crystals of metal solid solutions

The following definitions were sought in the research on single crystals of metal solid solutions: (1) the influence of convection and/or gravity present during crystallization on the substructure of a metal solid solution; (2) the influence of a magnetic field applied during crystallization on the substructure of a metal solid solution; and (3) requirements for a space flight experiment to verify the results. Growth conditions for the selected silver-zinc alloy system are described, along with pertinent technical and experimental details of the project

Elemental bio-imaging of thorium, uranium, and plutonium in tissues from occupationally exposed former nuclear workers

Internal exposure from naturally occurring radionuclides (including the inhaled long-lived actinides 232Th and 238U) is a component of the ubiquitous background radiation dose (National Council on Radiation Protection and Measurements. Ionizing radiation exposure of the population of the United States; NCRP Report No. 160; NCRP: Bethesda, MD, 2009). It is of interest to compare the concentration distribution of these natural ?-emitters in the lungs and respiratory lymph nodes with those resulting from occupational exposure, including exposure to anthropogenic plutonium and depleted and enriched uranium. This study examines the application of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICPMS) to quantifying and visualizing the mass distribution of uranium and thorium isotopes from both occupational and natural background exposure in human respiratory tissues and, for the first time, extends this application to the direct imaging of plutonium isotopes. Sections of lymphatic and lung tissues taken from deceased former nuclear workers with a known history of occupational exposure to specific actinide elements (uranium, plutonium, or americium) were analyzed by LA-ICPMS. Using a previously developed LA-ICPMS protocol for elemental bio-imaging of trace elements in human tissue and a new software tool, we generated images of thorium (232Th), uranium (235U and 238U), and plutonium (239Pu and 240Pu) mass distributions in sections of tissue. We used a laboratory-produced matrix-matched standard to quantify the 232Th, 235U, and 238U concentrations. The plutonium isotopes 239Pu and 240Pu were detected by LA-ICPMS in 65 ?m diameter localized regions of both a paratracheal lymph node and a sample of lung tissue from a person who was occupationally exposed to refractory plutonium (plutonium dioxide). The average (overall) 239Pu concentration in the lymph node was 39.2 ng/g, measured by high purity germanium (HPGe) ?-spectrometry (Lynch, T. P.; Tolmachev, S. Y.; James, A. C. Radiat. Prot. Dosim. 2009, 134, 94?101). Localized mass concentrations of thorium (232Th) and uranium (238U) in lymph node tissue from a person not occupationally exposed to these elements (chronic natural background inhalation exposure) ranged up to 400 and 375 ng/g, respectively. In lung samples of occupationally nonexposed to thorium and uranium workers, 232Th and 238U concentrations ranged up to 200 and 170 ng/g, respectively. In a person occupationally exposed to air-oxidized uranium metal (Adley, F. E.; Gill, W. E.; Scott, R. H. Study of atmospheric contaminiation in the melt plant buiding. HW-23352(Rev.); United States Atomic Energy Commission: Oakridge, TN, 1952, p 1?97), the maximum 235U and 238U isotopic mass concentrations in a lymph node, measured at higher resolution (with a 30 ?m laser spot diameter), were 70 and 8500 ng/g, respectively. The ratio of these simultaneously measured mass concentrations signifies natural uranium. The current technique was not sufficiently sensitive, even with a 65 ?m laser spot diameter, to detect 241Am (at an overall tissue concentration of 0.024 ng/g, i.e., 3 Bq/g). © 2010 American Chemical Society

Shielding effectiveness of non-woven carbon fibre sheets : modelling the microstructure

This paper describes work undertaken to understand how the structure of a nonwoven carbon fibre material determines its shielding effectiveness, including the effects of fibre orientation, and contact resistance. In order to facilitate understanding of the material behaviour, software has been written to generate Monte Carlo Models (MCMs) of the material structure. The results of our MCMs are compared with measurements and some empirical expressions

Shielding Effectiveness and Sheet Conductance of Nonwoven Carbon-Fiber Sheets

Nonwoven carbon-fiber sheets are often used to form a conductive layer in composite materials for electromagnetic shielding and other purposes. While a large amount of research has considered the properties of similar idealized materials near the percolation threshold, little has been done to provide validated analytic models suitable for materials of practical use for electromagnetic shielding. Since numerical models consume considerable computer resource and do not provide the insight which enables improved material design, an analytic model is of great utility for materials development. This paper introduces a new theoretical model for the sheet conductance of nonwoven carbon-fiber sheets built on the theory of percolation for 2-D conducting stick networks. The model accounts for the effects of sample thickness, fiber angle distribution, and contact conductance on the sheet conductance. The theory shows good agreement with Monte Carlo simulations and measurements of real materials in the supercritical percolation regime where the dimensionless areal concentration of fibers exceeds about 50. The theoretical model allows the rapid prediction of material shielding performance from a limited number of manufacturing parameters

Eigenvalue spectra of complex networks

We examine the eigenvalue spectrum, (), of the adjacency matrix of a random scale-free network with an average of p edges per vertex using the replica method. We show how in the dense limit, when p , one can obtain two relatively simple coupled equations whose solution yields () for an arbitrary complex network. For scale-free graphs, with degree distribution exponent , we obtain an exact expression for the eigenvalue spectrum when = 3 and show that () ~ 1/2-1 for large . In the limit we recover known results for the Erdös–Rényi random graph