430 research outputs found

    Moringa oleifera: Resource management and multiuse life tree

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    Moringa oleifera Lamarck (Moringaceae family) is a plant native from the Western and sub-Himalayan parts of Northwest India, Pakistan and Afghanistan. This species is widely cultivated across Africa, South-East Asia, Arabia, South America and Caribbean Islands. M. oleifera culture is also being distributed in the Semi-Arid Northeast of Brazil. It is a multiuse life tree with great environmental economic importance in industrial and medical areas. This review reports different purposes of M. oleifera including sustaining environmental resources, soil protection and shelter for animals. This plant requires not much care and distinct parts have bioactive compounds. Moringa tissues used in human and animal diets, also withdraw pollutants from water. The seeds with coagulant properties used in water treatment for human consumption, remove waste products like surfactants, heavy metals and pesticides. The oil extracted from seeds is used in cosmetic production and as biodiesel. M. oleifera tissues also contain proteins with different biological activities, including lectins, chitin-binding proteins, trypsin inhibitors, and proteases. The lectins are reported to act as insecticidal agents against Aedes aegypti (vector of dengue, chikungunya and yellow fevers) and Anagasta kuehniella (pest of stored products) and also showed water coagulant, antibacterial and blood anticoagulant activities. The presence of trypsin inhibitors has been reported in M. oleifera leaves and flowers. The inhibitor from flowers is toxic to larvae of A. aegypti. The flowers also contain caseinolytic proteases that are able to promote clotting of milk. In this sense, M. oleifera is a promising tree from a biotechnological point of view, since it has shown a great variety of uses and it is a source of several compounds with a broad range of biological activities.Conselho Nacional de Desenvolvimento Científico e Tecnológico for fellowship (LCBBC) and to the Foundation for Science and Technology, POPH/FSE (AFSS

    Utilização de chuveiros na sala pré-ordenha e sua influência na produtividade de búfalas da raça Murrah (Bubalus bubalis).

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    O estudo foi realizado com 64 búfalas, em dois tratamentos (1 e 2), durante seis dias. No primeiro, antes da ordenha, os animais permaneceram 1h em sala de espera com acesso ao chuveiro No segundo, os animais não tiveram acesso ao chuveiro. Foram registrados Índice de Temperatura de Globo e Umidade (ITGU) e a Umidade Relativa (UR) das salas de ordenha e de espera, Temperatura de Corpo (TC), Frequência Respiratória (FR), Reatividade (REAT), Nível de Estresse (NEST) e a Produção Diária de Leite (PL). Os dados foram analisados com o auxílio do programa SAS. O efeito da FR não foi significativo (P>0,57) sobre a PL. Não houve diferença significativa na PL para a REAT. O NEST foi o mesmo nos dois tratamentos. As regressões da característica (PL_AJ) sobre ITGU Interno (ITGU_I), ITGU Externo (ITGU_E), TC e UR não foram significativas (P>0,12). No tratamento 2 foram observados maior número de animais em classes de estresse, verificou-se que o tratamento 1 possibilitou a mudança ( alguns animais) da zona de alerta ou estresse para a zona de conforto térmico em relação ao ITGU_I. No tratamento 2, todos os animais estavam em estresse térmico. Os animais submetidos ao estresse calórico em ambas as situações utilizaram com eficiência o seu sistema termorregulador, sem interferir na produção de leite

    Anticoagulation in hospitalized patients

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    Os eventos tromboembólicos, principalmente o tromboembolismo venoso, acrescem importante morbidade e mortalidade e representam a segunda causa mais comum de complicações em pacientes hospitalizados. A anticoagulação é o tratamento padrão nestes casos, porém frequentemente seu manejo é complexo e requer conhecimento adequado tanto da farmacologia das drogas quanto da fisiologia da coagulação.Este artigo revê alguns pontos essenciais para quem lida com esses eventos e fornece noções práticas do manejo dos antagonistas da vitamina K e dos anticoagulantes parenterais.Thromboembolic events, particularly venous thromboembolism, add significant morbidity and mortality and represent the second most common cause of complications in hospitalized patients. Anticoagulation is the standard treatment in these cases, but often their management is complex and requires adequate knowledge of both the pharmacology of drugs and the physiology of coagulation. This article reviews some key points to those who deal with these events and provides practical notions of management of vitamin K antagonists and parenteral anticoagulants

    Coagulant and antibacterial activities of the water-soluble seed lectin from Moringa oleifera

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    Aims: The aim of this work was to analyse the coagulant and antibacterial activities of lectin isolated from Moringa oleifera seeds that are used for water treatment. Methods and Results:  The water-soluble M. oleifera lectin (WSMoL) was separated from nonhemagglutinating components (NHC) by chitin chromatography. WSMoL fluorescence spectrum was not altered in the presence of ions that are often present in high concentrations in polluted waters. Seed extract, NHC and WSMoL showed coagulant activity on a turbid water model. Both NHC and WSMoL reduced the growth of Staphylococcus aureus, but only WSMoL caused a reduction in Escherichia coli. WSMoL was also more effective in reducing the growth of ambient lake water bacteria. Conclusions:  Data obtained from this study indicate that WSMoL is a potential natural biocoagulant for water, reducing turbidity, suspended solids and bacteria. Significance and Impact of the Study: Moringa oleifera seeds are a material effective in the treatment of water.The authors express their gratitude to the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for research grants and fellowship (LCBBC, MLVO and PMGP), the Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE) and the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) for financial support. Authors are grateful to Maria Barbosa Reis da Silva for the technical assistance and to David Pillard and Felix Nonnenmacher for English editing

    Validation of TerraClass mapping for the Municipality of Paragominas state of Pará

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    This work aims to evaluate the TerraClass mapping for the year 2014, in the municipality of Paragominas, State of Pará. The validation was made by comparing the mapping with the observations found in the field. Images of the Satétile Landsat-8, OLI sensor of the year 2014, path/row 222/062, 222/063, 223/062 and 223/063 were used to aid in the field. Using this data it was possible to analyze the main representative classes in the area, including agriculture, urban area, forest, clean pasture, dirty pasture, reforestation, regeneration with pasture and secondary vegetation. The secondary vegetation presented 2,198.16 km², clean pasture with 3,332.29 km², agriculture with 896.75 km² and the forest occupying 54.21% of the total area of Paragominas. The overall concordance index was 86%, corroborating the reliability of the mapping performed. The average error was 6% and the total value of discordance was of 14%. Concerning the secondary vegetation, pasture, agriculture, urban area and forest classes, they presented concordance higher to 50%, while regeneration with pasture and reforestation presented greater intensity of omission with 40,57% and 76,31% respectively. Inclusion errors were less than 40% for the secondary vegetation, pasture regeneration, clean pasture and dirty pasture classes. The field work was essential to validate and analyze the accuracy of the 2014 TerraClass Project for the studied region, which becomes important for the understanding of the dynamics of land use

    Purification, characterization and termiticidal activity of Moringa oleifera flower peptides

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    Moringa oleifera (Moringaceae) is a tree cultivated in tropical regions . Their flowers are consumed as food, mainly in the Philippines. Antinutritional factors such as lectins and protease inhibitors are common in plant tissues and may disrupt nutrient digestion. This study aimed to isolate and characterize bioactive peptides in M. oleifera flowers. Dried flowers (10 g) were extracted with 0.15 M NaCl (100 mL). A saline extract from M. oleifera flowers (EMo) was treated with 60 and 90% ammonium sulfate. The 60% precipitated fraction (F0-60) was assessed on hemagglutinating, trypsin inhibitor, caseinolytic and endopeptidase activities. The 90% precipitated fraction (F0-90) was submitted to termiticidal activity assay against Nasutitermes corniger (Termitidae), workers and soldiers. None hemagglutinating activity was detected. A M. oleifera flower trypsin inhibitor (MoFTI) was purified on trypsin-Sepharose affinity column and partially characterized. MoFTI activity was stable until 90 °C and lower after heating to 100 ºC (65%). MoFTI was active at pH range 4-8. MoFTI SDS-PAGE showed three polypeptide bands of 14, 22 and 30 kDa. F0-60 showed caseinolytic activity and endopeptidase activity to hydrolyze a-N-benzoyl-DL-arginine-p-nitroanilide. The enzyme adsorbed on ion exchange column, CM-cellulose, was eluted with 1 M NaCl; SDS-PAGE revealed the presence of two polypeptide bands with molecular weights of 14 and 20 kDa. F0-90 promoted mortality of N. corniger workers, but did not affect soldier survival. In conclusion, the M. oleifera flowers contained enzymes, trypsin inhibitor and termiticidal activity.info:eu-repo/semantics/publishedVersio
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