Uji Fitokimia dan Profil Kromatografi Lapis Tipis Senyawa Metabolit Sekunder dari Ekstrak Daun Calathea Silver

Daun Calathea Silver merupakan tanaman hias yang memiliki warna dasar ungu. Jika tanaman memiliki warna ungu, maka kemungkinan memiliki aktivitas antioksidan yang tinggi dan senyawa metabolit yang banyak terkandung adalah golongan flavonoid. Penelitian ini bertujuan sebagai skrinning awal dalam menentukan kandungan senyawa metabolit sekunder yang ada di dalam ekstrak etanol daun Calathea silver berdasarkan uji reagen fitokimia dan profil persebaran senyawa kimia menggunakan kromatografi lapis tipis. Hasil penelitian ini adalah senyawa metabolit sekunder yang terkandung dalam ekstrak etanol daun Calathea silver adalah golongan flavonoid, alkaloid, tanin, dan steroid. Berdasarkan profil kromatografinya terdapat 5 nilai Rf yaitu, 0,4; 0,5; 0,7; 0,8 dan 0,9. Eluen yang baik dan yang dapat digunakan untuk proses pemisahan selanjutnya adalah eluen campuran kloroform : metanol dengan perbandingan 10:1

HEALTH EDUCATION 6 STEPS HAND WASHING, USE OF MASK, AND HANDSANITIZER IN RA AL HUDA PAYAMAN, NGANJUK REGENCY, EAST JAVA

Pada tanggal 2 Maret 2020, kasus pertama COVID-19 terdeteksi di Indonesia; per tanggal 8 Mei 2020, ada 12.776 kasus dan 930 kematian dilaporkan terjadi di 34 provinsi. Pandemi ini telah menjadi ancaman signifikan bagi kesehatan fisik dan mental manusia dan secara dramatis mempengaruhi kehidupan sehari-hari dengan implikasi psikososial dalam skala global. Berbagai upaya untuk mengendalikan pandemi tersebut menimbulkan dampak signifikan di sektor ekonomi, kegiatan sehari-hari, dan seluruh aspek kehidupan anak. Penutupan sekolah dapat memperburuk kesenjangan akses pendidikan. Anak-anak menghadapi beragam kesulitan dalam mengakses dan mendapatkan pendidikan berkualitas, bahkan sejak sebelum pandemi. Fakultas Ilmu Kesehatan Universitas Kadiri melalui program Pengabdian Masyarakat ini dapat ikut berkontribusi dalam upaya untuk memutus mata rantai penyebaran COVID-19 pada anak-anak di sekolah dengan cara memberikan pendidikan kesehatan kepada siswa taman kanak-kanak tentang cuci tangan 6 langkah, pemakaian masker, dan handsanitizer. Melalui kegiatan simulasi ini anak sekolah dapat memperoleh pengetahuan dan teknik cuci tangan yang benar, memakai masker dengan benar, dan memakai handsanitizer dengan tepat. Simulasi ini diharapkan dapat menekan angka kejadian penularan COVID-19 dan penyakit pada anak sekolah seperti diare dan kecacingan akibat bakteri yang masuk ke dalam tubuh ketika anak tidak mencuci tangan

EDUKASI ORANGTUA SISWA MENGENAI CARA PEMBUATAN HANDSANITIZER DI RA AL HUDA PAYAMAN, KABUPATEN NGANJUK, JAWA TIMUR

Handsanitizer atau yang juga dikenal sebagai antiseptik tangan atau hand rub adalah produk yang dioleskan ke tangan untuk menghilangkan patogen umum di tangan. Handsanitizer biasanya tersedia dalam bentuk busa, gel, atau cair dan direkomendasikan untuk digunakan ketika tidak ada air dan sabun atau ada masalah medis lainnya (misalnya, menyebabkan retakan pada kulit). Munculnya COVID-19 ini adanya persediaan handsanitizer di toko-toko sering menipis dan harganya mahal karena semua masyarakat sedang membutuhkan handsanitizer untuk mencegah COVID-19. Fakultas Ilmu Kesehatan Universitas Kadiri mengadakan penyuluhan pembuatan handsanitizer. Melihat situasi saat ini masih besar harapan bahwa taraf kehidupan orangtua siswa di RA Al Huda, Payaman, Kabupaten Nganjuk, Jawa Timur ini dapat ditingkatkan melalui kegiatan masyarakat yang dapat membantu melindungi sesama keluarga dari COVID-19, salah satu caranya dengan penggunaan handsanitizer yang berlebihan tidak baik untuk kesehatan kulit dan setelah pemakain handsanitizer berulang kali harus tetap cuci tangan.

Uji Potensi Triterpenoid dari Kulit Batang Waru Jawa (Hibiscus tiliaceus L.) sebagai Kandidat Antiinflamasi pada Mencit (Mus musculus) Model Rheumatoid Arthritis Berbasis in Silico

Waru jawa (Hibiscus tiliaceus L.) is a plant that functions as an anti-inflammatory. Compounds from the waru jawa plant, especially the bark can be grouped into alkaloids, flavonoids, triterpenoids, and steroids. The content of triterpenoids from the waru jawa stem bark was tested for their biochemical activity, so that it is expected that the bark of Javanese waru stems can be used optimally. This study aims to determine the potential of triterpenoids from waru jawa stem bark as anti-inflammatory candidates in mice (Mus musculus) rheumatoid arthritis model in silico-based. Potential analysis of triterpenoids in mice was carried out using the STITCH, which is a database of known and predicted interactions between chemicals and proteins found in living things. Interactions in question are physical and functional associations, the data contained in STITCH comes from computational predictions, transfer of knowledge between organisms, and from interactions collected from other databases. Analysis of the triterpenoid mechanism of waru jawa stem bark against rheumatoid arthritis model mice in silico-based using the bioinformatics application Kegg. Based on the results of the analysis, it can be seen that triterpenoids derived from waru jawa stem bark sources play an important role in reducing inflammation. The triterpenoids target NF-κB, leading to its downregulation. Triterpenoids have been found to have many functions, although their effective concentrations for various cellular effects may vary widely. Depending upon the dose administered, triterpenoids can induce anti-inflammatory, proliferation-arresting, apoptotic effects, cytoprotective, and tumor-differentiating

In Silico BCell Epitope Design of Zika Virus Vaccine Using “Zika Virus Isolate Zika Virus/H sapiensTc/Tha/2006/CVD_06020 Complete Genome”

ZIKV has been found in the cerebrospinal fluid (CSF) and brains of adults infected with viruses that manifest neurological disorders. ZIKV is a mosquito-borne single-stranded RNA virus, which belongs to the family Flaviviridae. The efforts of the scientific community have rapidly increased knowledge about this virus. However, understanding the complexities of ZIKV infection, transmission and pathogenesis remains an urgent challenge. Therefore, it is critical to study competent vectors and natural reservoirs for ZIKV, viral genetic diversity and flavivirus coinfection. Due to the great challenges to develop a ZIKV vaccine, it is still not possible to be immunized against ZIKV infection and related pathologies. The methods are nucleotide search for the Zika virus was carried out in silico, using the NCBI bioinformatics application by providing access to biomedical and genomic information. /H. sapiens-tc/THA/2006/CVD_06-020, the complete genome was then searched for FASTA, then prediction of vaccine epitope using the IEDB. The vaccine candidate peptides were analyzed for their antigenicity using VaxiJen. Proteins were classified by AllerTop to known allergenicity, then ToxinPred to predict and design toxic/non-toxic peptides. There are 30 peptide sequences are predicted to be a candidate of peptides B-cell epitope zika virus vaccine design using “zika virus isolate zika virus/H. sapiens-tc/THA/2006/CVD_06-020, complete genome”

Older Blood Is Associated With Increased Mortality and Adverse Events in Massively Transfused Trauma Patients: Secondary Analysis of the PROPPR Trial

© 2018 American College of Emergency Physicians Study objective: The transfusion of older packed RBCs may be harmful in critically ill patients. We seek to determine the association between packed RBC age and mortality among trauma patients requiring massive packed RBC transfusion. Methods: We analyzed data from the Pragmatic, Randomized Optimal Platelet and Plasma Ratios trial. Subjects in the parent trial included critically injured adult patients admitted to 1 of 12 North American Level I trauma centers who received at least 1 unit of packed RBCs and were predicted to require massive blood transfusion. The primary exposure was volume of packed RBC units transfused during the first 24 hours of hospitalization, stratified by packed RBC age category: 0 to 7 days, 8 to 14 days, 15 to 21 days, and greater than or equal to 22 days. The primary outcome was 24-hour mortality. We evaluated the association between transfused volume of each packed RBC age category and 24-hour survival, using random-effects logistic regression, adjusting for total packed RBC volume, patient age, sex, race, mechanism of injury, Injury Severity Score, Revised Trauma Score, clinical site, and trial treatment group. Results: The 678 patients included in the analysis received a total of 8,830 packed RBC units. One hundred patients (14.8%)died within the first 24 hours. On multivariable analysis, the number of packed RBCs greater than or equal to 22 days old was independently associated with increased 24-hour mortality (adjusted odds ratio [OR]1.05 per packed RBC unit; 95% confidence interval [CI]1.01 to 1.08): OR 0.97 for 0 to 7 days old (95% CI 0.88 to 1.08), OR 1.04 for 8 to 14 days old (95% CI 0.99 to 1.09), and OR 1.02 for 15 to 21 days old (95% CI 0.98 to 1.06). Results of sensitivity analyses were similar only among patients who received greater than or equal to 10 packed RBC units. Conclusion: Increasing quantities of older packed RBCs are associated with increased likelihood of 24-hour mortality in trauma patients receiving massive packed RBC transfusion (≥10 units), but not in those who receive fewer than 10 units