Exosomes released from breast cancer carcinomas stimulate cell movement

For metastasis to occur cells must communicate with to their local environment to initiate growth and invasion. Exosomes have emerged as an important mediator of cell-to-cell signalling through the transfer of molecules such as mRNAs, microRNAs, and proteins between cells. Exosomes have been proposed to act as regulators of cancer progression. Here, we study the effect of exosomes on cell migration, an important step in metastasis. We performed cell migration assays, endocytosis assays, and exosome proteomic profiling on exosomes released from three breast cancer cell lines that model progressive stages of metastasis. Results from these experiments suggest: (1) exosomes promote cell migration and (2) the signal is stronger from exosomes isolated from cells with higher metastatic potentials; (3) exosomes are endocytosed at the same rate regardless of the cell type; (4) exosomes released from cells show differential enrichment of proteins with unique protein signatures of both identity and abundance. We conclude that breast cancer cells of increasing metastatic potential secrete exosomes with distinct protein signatures that proportionally increase cell movement and suggest that released exosomes could play an active role in metastasis

Multistep Measurement of Plantar Pressure Alterations Using Metatarsal Pads

Metatarsal pads are frequently prescribed for nonoperative management of metatarsalgia due to various etiologies. When appropriately placed, they are effective in reducing pressures under the metatarsal heads on the plantar surface of the foot. Despite the positive clinical reports that have been cited, there are no quantitative studies documenting the load redistribution effects of these pads during multiple step usage within the shoe environment. The objective of this study was to assess changes in plantar pressure metrics resulting from pad use. Ten normal adult male subjects were tested during a series of 400-step trials. Pressures were recorded from eight discrete plantar locations at the hindfoot, midfoot, and forefoot regions of the insole. Significant increases in peak pressures, contact durations, and pressure-time integrals were noted at the metatarsal shaft region with pad use (P ≤ .05). Statistically significant changes in metric values were not seen at the other plantar locations, although metatarsal pad use resulted in mild decreases in mean peak pressures at the first and second metatarsal heads and slight increases laterally. Contact durations decreased at all metatarsal head locations, while pressure-time integrals decreased at the first, second, third, and fourth metatarsal heads. A slight increase in pressure-time integrals was seen at the fifth metatarsal head. The redistribution of plantar pressures tended to relate not only to the dimensions of the metatarsal pads, but also to foot size, anatomic foot configuration, and pad location. Knowledge of these parameters, along with careful control of pad dimensions and placement, allows use of the metatarsal pad as an effective orthotic device for redistributing forefoot plantar pressures

Higher Education, de-centred subjectivities and the emergence of a pedagogical self among Black and Muslim students

This article explores late modern Black and Muslim young men’s and women’s experiences of higher education. Carrying out qualitative research with 14 male and female young people, these students claimed that their Youth and Community Work course at their university made available an alternative representational space, enabling them to develop a major transformation of their sense of identity and self. In deploying the term pedagogical self, we are attempting to capture their naming pedagogy as central, in their terms, to the ‘reinvention of their selves’. We conclude by suggesting that our research participants’ narratives are located within an exploration of late modern identity and the self in higher education. In turn, this enables us to reflect on a generational shift in meanings around racialization and difference in thinking about the future of higher education in Britain

Excitation Functions of Stopping Power and Flow in Relativistic Heavy-Ion Collisions

Using a relativistic transport (ART) model, we study the stopping power, the formation of superdense hadronic matter as well as the strength of transverse and radial flow in central Au+Au collisions at beam momentum from 2 to 12 GeV/c per nucleon. We find that complete stopping is achieved in the whole beam momentum range. In particular, the proton rapidity distribution scaled by the beam rapidity is independent of the beam momentum, and this is in agreement with the experimental findings. Also, a large volume of superdense hadronic matter with a local energy density exceeding that expected for the transition of a hadronic matter to the quark-gluon plasma is formed in collisions at beam momenta greater than 8 GeV/c per nucleon. Furthermore, it is found that the transverse flow in these collisions is sensitive to the nuclear equation of state and decreases with increasing beam momentum. On the other hand, the radial flow is insensitive to the equation of state, and its strength increases with beam momentum.Comment: Talk given at NN97, Gatlinburg, Tennessee June 2-6,1997. To appear in the proc. in Nucl. Phys.

BES3 time of flight monitoring system

A Time of Flight monitoring system has been developed for BES3. The light source is a 442-443 nm laser diode, which is stable and provides a pulse width as narrow as 50 ps and a peak power as large as 2.6 W. Two optical-fiber bundles with a total of 512 optical fibers, including spares, are used to distribute the light pulses to the Time of Flight counters. The design, operation, and performance of the system are described.Comment: 8 pages 16 figures, submitted to NI

Resonance raman characterization of the forms of ground-state 8-substituted 7-hydroxyquinoline caged acetate compounds in aqueous solutions

Monday Poster Session: Resonance Raman in Biological and Chemical Systems (MP22) - Poster Number: 0978-substituted 7-hydroxyquinolines, like 8-chloro-7-hydroxyquinoline (CHQ) and 8-cyano-7-hydroxyquinoline (CyHQ), are able to be useful for 1PE and 2PE and their acetate acids CHQ−OAc and CyHQ−OAc were also able to undergo photolysis reactions in neutral aqueous buffer solutions. To examine the substituent effect on the relative populations of the forms of the ground state species of 8-substituted 7-hydroxyquinolines, ultraviolet absorption and resonance Raman spectroscopy experiments were done for CHQ–OAc and CyHQ–OAc in differnt solutions.postprintThe 22nd International Conference on Raman Spectroscopy (ICORS 2010), Boston, MA., 8-13 August 2010

MicroRNAs in lung cancer

Lung cancer (LC) is a serious public health problem responsible for the majority of cancer deaths and comorbidities in developed countries. Tobacco smoking is considered the main risk factor for LC; however, only a few smokers will be affected by this cancer. Current screening methods are focused on identifying the early stages of this malignancy. Thus, new data concerning the roles of microRNA alterations in inflammation, epithelial-mesenchymal transition and lung disease have increased hope about LC pathogenesis, diagnosis, treatment and prognosis. MicroRNA mechanisms include angiogenesis promotion, cell cycle regulation by modulating cellular proliferation and apoptosis, and migration and invasion inhibition. In this context, this manuscript reviews the current information about many important microRNAs as they relate to the initiation and progression of LC.info:eu-repo/semantics/publishedVersio

Contribution of the Myosin Binding Protein C Motif to Functional Effects in Permeabilized Rat Trabeculae

Myosin binding protein C (MyBP-C) is a thick-filament protein that limits cross-bridge cycling rates and reduces myocyte power output. To investigate mechanisms by which MyBP-C affects contraction, we assessed effects of recombinant N-terminal domains of cardiac MyBP-C (cMyBP-C) on contractile properties of permeabilized rat cardiac trabeculae. Here, we show that N-terminal fragments of cMyBP-C that contained the first three immunoglobulin domains of cMyBP-C (i.e., C0, C1, and C2) plus the unique linker sequence termed the MyBP-C “motif” or “m-domain” increased Ca2+ sensitivity of tension and increased rates of tension redevelopment (i.e., ktr) at submaximal levels of Ca2+. At concentrations ≥20 μM, recombinant proteins also activated force in the absence of Ca2+ and inhibited maximum Ca2+-activated force. Recombinant proteins that lacked the combination of C1 and the motif did not affect contractile properties. These results suggest that the C1 domain plus the motif constitute a functional unit of MyBP-C that can activate the thin filament