22 research outputs found
Trimethylamine-N-Oxide Promotes Vascular Calcification Through Activation of NLRP3 (Nucleotide-Binding Domain, Leucine-Rich-Containing Family, Pyrin Domain-Containing-3) Inflammasome and NF-ÎşB (Nuclear Factor ÎşB) Signals
MoS<sub>2</sub>–Gd Chelate Magnetic Nanomaterials with Core–Shell Structure Used as Contrast Agents in <i>in Vivo</i> Magnetic Resonance Imaging
Despite their frequent usages as
contrast agents for <i>in vivo</i> MRI imaging, paramagnetic
molecules continue to suffer from low resolution, physicochemical
instability, and high toxicity. Herein, we present a molybdenum disulfide
and gadolinium complex, as an alternative core–shell magnetic
nanomaterial that exhibits enhanced paramagnetic property; 4.5-times
longer water proton spin–lattice relaxation time (<i>T</i><sub>1</sub>) when compared to commercial gadolinium contrast agents;
as well as lowered toxicity, extended blood circulation time, increased
stability, and desirable excretion characteristic. Transmission electron
microscopy (TEM) revealed smooth core–shell nanoparticles 100
nm in size with a shell width of approximately 10 nm. These findings
suggest that the synthesized nanomaterial possesses high potential
as a positive contrast agent for the enhancement of MRI imaging
Resonance energy transfer, pH-induced folded states and the molecular interaction of human serum albumin and icariin
Delineation of groundwater recharge zones and identification of artificial recharge sites in West Medinipur district, West Bengal, using RS, GIS and MCDM techniques
Cartography of Pathway Signal Perturbations Identifies Distinct Molecular Pathomechanisms in Malignant and Chronic Lung Diseases
Vertically-aligned 1T/2H-MS2 (MÂ =Â Mo, W) nanosheets for surface-enhanced Raman scattering with long-term stability and large-scale uniformity
Mechanism(S) Involved in the Colon-Specific Expression of the Thiamine Pyrophosphate (Tpp) Transporter
Microbiota of the large intestine synthesizes considerable amount of vitamin B1 (thiamine) in the form of thiamine pyrophosphate (TPP). We have recently demonstrated the existence of an efficient and specific carrier-mediated uptake process for TPP in human colonocytes, identified the TPP transporter (TPPT) involved (product of the SLC44A4 gene), and shown that expression of TPPT along the gastrointestinal (GI) tract is restricted to the colon. Our aim in this study was to determine the molecular basis of the colon-specific expression of TPPT focusing on a possible epigenetic mechanism. Our results showed that the CpG island predicted in the SLC44A4 promoter is non-methylated in the human colonic epithelial NCM460 cells, but is hyper-methylated in the human duodenal epithelial HuTu80 cells (as well as in the human retinal pigment epithelial ARPE19 cells). In the mouse (where TPPT expression in the GI tract is also restricted to the colon), the CpG island predicted in the Slc44a4 promoter is non-methylated in both the jejunum and colon, thus arguing against possible contribution of DNA methylation in the colon-specific expression of TPPT. A role for histone modifications in the tissue-specific pattern of Slc44a4 expression, however, was suggested by the findings that in mouse colon, histone H3 in the 5'-regulatory region of Slc44a4 is tri-methylated at lysine 4 and acetylated at lysine 9, whereas the tri-methylation at lysine 27 modification was negligible. In contrast, in the mouse jejunum, histone H3 is hyper-trimethylated at lysine 27 (repressor mark). Similarly, possible involvement of miRNA(s) in the tissue-specific expression of TPPT was also suggested by the findings that the 3'-UTR of SLC44A4 is targeted by specific miRNAs/RNA binding proteins in non-colonic, but not in colonic, epithelial cells. These studies show, for the first time, epigenetic mechanisms (histone modifications) play a role in determining the tissue-specific pattern of expression of TPPT in the GI tract