Requirements traceability in model-driven development: Applying model and transformation conformance

The variety of design artifacts (models) produced in a model-driven design process results in an intricate relationship between requirements and the various models. This paper proposes a methodological framework that simplifies management of this relationship, which helps in assessing the quality of models, realizations and transformation specifications. Our framework is a basis for understanding requirements traceability in model-driven development, as well as for the design of tools that support requirements traceability in model-driven development processes. We propose a notion of conformance between application models which reduces the effort needed for assessment activities. We discuss how this notion of conformance can be integrated with model transformations

Enumeration and characterization of arsenic-tolerant diazotrophic bacteria in a long-term heavy-metal-contaminated soil

The abundance of arsenic-tolerant diazotrophic bacteria was compared in a long-term contaminated soil versus a non-contaminated one. In addition, the characterization of tolerant diazotrophic bacteria was carried out. Differences in the number of heterotrophic N2 fixers were found between soils. Contaminated soil showed a decrease in the microbial population size of about 80%, confirming the great sensitivity of this group of soil bacteria to metals. However, quantitative analysis of the response to increased doses of arsenic reveals that the proportion of the culturable diazotrophic community tolerant to arsenic was identical for both soils (contaminated and non-contaminated). Twentytwo arsenic-tolerant diazotrophic isolates were obtained and further characterized. 16S ribosomal DNA sequence analysis revealed that these bacterial isolates were distributed among four taxons (Actinobacteria, Firmicutes, γ-Proteobacteria, and β-Proteobacteria). Most genera recovered from the contaminated soil were also found in the uncontaminated soi

A whole genome screen for association with multiple sclerosis in portuguese patients

Multiple sclerosis (MS) is common in Europe affecting up to 1:500 people. In an effort to identify genes influencing susceptibility to the disease, we have performed a population-based whole genome screen for association. In this study, 6000 microsatellite markers were typed in separately pooled DNA samples from MS patients (n = 188) and matched controls (n = 188). Interpretable data was obtained from 4661 of these markers. Refining analysis of the most promising markers identified 10 showing potential evidence for association.SERONO (Portugal).Fundação para a Ciência e a Tecnologia (FCT) - grant FRH/BD/9111/2002.British Council/ICCTI.Wellcome Trust, Multiple Sclerosis Societies of the United States and Great Britain, Multiple Sclerosis International Federation - GAMES project - grant 057097

Gauge and Scheme Dependence of Mixing Matrix Renormalization

We revisit the issue of mixing matrix renormalization in theories that include Dirac or Majorana fermions. We show how a gauge-variant on-shell renormalized mixing matrix can be related to a manifestly gauge-independent one within a generalized ${\bar {\rm MS}}$ scheme of renormalization. This scheme-dependent relation is a consequence of the fact that in any scheme of renormalization, the gauge-dependent part of the mixing-matrix counterterm is ultra-violet safe and has a pure dispersive form. Employing the unitarity properties of the theory, we can successfully utilize the afore-mentioned scheme-dependent relation to preserve basic global or local symmetries of the bare Lagrangian through the entire process of renormalization. As an immediate application of our study, we derive the gauge-independent renormalization-group equations of mixing matrices in a minimal extension of the Standard Model with isosinglet neutrinos.Comment: 31 pages, LaTeX, uses axodraw.st

Body condition score, weight variation and reproductive performance of beef cows in rangelands from the Pantanal region

Objetivou-se avaliar a influência do escore de condição corporal (ECC) sobre a eficiência reprodutiva de vacas de cria nos períodos pré e pós-parto e a probabilidade de parição, usando o escore de condição corporal e a variação ponderal no período crítico do ciclo reprodutivo. Utilizaram-se 248 vacas multíparas mantidas em pastagens nativas distribuídas em quatro estratégias de suplementação: mistura mineral (controle); suplemento farelado; suplemento líquido; suplemento líquido para vacas e bezerros. As avaliações de peso e ECC, na escala de 1 a 9, foram realizadas no período crítico: em agosto (período pré-parto, 60-90 dias antes da parição) e em janeiro (período pós-parto e reprodutivo) durante quatro anos consecutivos. Para a análise dos anos consecutivos de reprodução, criou-se um escore de reprodução, ou seja, somou-se o número de parições de cada vaca durante os quatro anos pecuários avaliados. O escore de condição corporal nos períodos pré e pós-parto e a idade da vaca influenciaram o escore de reprodução. Portanto, para avaliação da probabilidade de parição, foram utilizadas todas as vacas, classificadas em paridas e vazias. Utilizando equações de estimação generalizadas (EEG), foi modelada a probabilidade de as matrizes serem classificadas como paridas considerando variáveis exploratórias ao longo do tempo. Para se obter probabilidade de parição superior a 80%, as vacas devem possuir escore de aproximadamente 5,5 no período pré-parto. Aproximadamente 60% das vacas paridas mantiveram ou ganharam peso no período crítico do ano, o que levou a maior probabilidade de reconcepção, similar ao observado para as vacas vazias, porém com probabilidade menor de parição. O período pré-parto é o melhor para estimar a probabilidade de parição das vacas por meio da avaliação do escore corporal. A seleção de vacas adaptadas às condições bioclimáticas do Pantanal aumenta a probabilidade de produção de um bezerro por vaca por ano. _________________________________________________________________________________ ABSTRACTThe aim of this study was to evaluate the influence of body condition score (BCS) on reproductive performance of beef cows before and after calving and to evaluate the calving probability using body condition score and weight variation during critical periods of the cow reproductive cycle. From 2001 to 2005, the body condition score was recorded in 248 multiparous cows raised in native pastures, submitted to different supplementation treatments (mineral mixture only - control; dry supplement; liquid supplement and liquid supplement for cow and calves). Weighing and body condition evaluation were made on a 1-9 point scale in August (prepartum period, 60-90 days before parturition) and January (postpartum and reproductive period). A reproduction score was created in function of the body scores in four consecutive years. Except for the treatment, the other factors (body condition score in the prepartum and postpartum periods and cow age) influenced the reproduction score. Thus, the calving probabilities were estimated for all cows. Generalized estimating equations (GEE) were used to evaluate the calving probability in function of the exploratory variables over time. Calving probability higher than 80% was obtained when cows scored 5,5 before calving. In relation to weight, the results showed that about 60% of calving cows maintained or gained weight in the postpartum period, leading to increase in the rebreeding probability. Similar situation occurred with single cows; however, the calving probability was lower. These results indicate that the calving probability using BCS is better estimated before calving. Furthermore, it indicates the presence of cows more adapted to the Pantanal climatic and feeding conditions than others

Phyllosticta citricarpa and sister species of global importance to Citrus.

Several Phyllosticta species are known as pathogens of Citrus spp., and are responsible for various disease symptoms including leaf and fruit spots. One of the most important species is P. citricarpa, which causes a foliar and fruit disease called citrus black spot. The Phyllosticta species occurring on citrus can most effectively be distinguished from P. citricarpa by means of multilocus DNA sequence data. Recent studies also demonstrated P. citricarpa to be heterothallic, and reported successful mating in the laboratory. Since the domestication of citrus, different clones of P. citricarpa have escaped Asia to other continents via trade routes, with obvious disease management consequences. This pathogen profile represents a comprehensive literature review of this pathogen and allied taxa associated with citrus, focusing on identification, distribution, genomics, epidemiology and disease management. This review also considers the knowledge emerging from seven genomes of Phyllosticta spp., demonstrating unknown aspects of these species, including their mating behaviour.TaxonomyPhyllosticta citricarpa (McAlpine) Aa, 1973. Kingdom Fungi, Phylum Ascomycota, Class Dothideomycetes, Order Botryosphaeriales, Family Phyllostictaceae, Genus Phyllosticta, Species citricarpa.Host rangeConfirmed on more than 12 Citrus species, Phyllosticta citricarpa has only been found on plant species in the Rutaceae.Disease symptomsP. citricarpa causes diverse symptoms such as hard spot, virulent spot, false melanose and freckle spot on fruit, and necrotic lesions on leaves and twigs.Useful websitesDOE Joint Genome Institute MycoCosm portals for the Phyllosticta capitalensis (https://genome.jgi.doe.gov/Phycap1), P. citriasiana (https://genome.jgi.doe.gov/Phycit1), P. citribraziliensis (https://genome.jgi.doe.gov/Phcit1), P. citrichinaensis (https://genome.jgi.doe.gov/Phcitr1), P. citricarpa (https://genome.jgi.doe.gov/Phycitr1, https://genome.jgi.doe.gov/Phycpc1), P. paracitricarpa (https://genome.jgi.doe.gov/Phy27169) genomes. All available Phyllosticta genomes on MycoCosm can be viewed at https://genome.jgi.doe.gov/Phyllosticta

Comprehensive clinical and molecular analysis of 12 families with type 1 recessive cutis laxa.

Autosomal recessive cutis laxa type I (ARCL type I) is characterized by generalized cutis laxa with pulmonary emphysema and/or vascular complications. Rarely, mutations can be identified in FBLN4 or FBLN5. Recently, LTBP4 mutations have been implicated in a similar phenotype. Studying FBLN4, FBLN5, and LTBP4 in 12 families with ARCL type I, we found bi-allelic FBLN5 mutations in two probands, whereas nine probands harbored biallelic mutations in LTBP4. FBLN5 and LTBP4 mutations cause a very similar phenotype associated with severe pulmonary emphysema, in the absence of vascular tortuosity or aneurysms. Gastrointestinal and genitourinary tract involvement seems to be more severe in patients with LTBP4 mutations. Functional studies showed that most premature termination mutations in LTBP4 result in severely reduced mRNA and protein levels. This correlated with increased transforming growth factor-beta (TGFβ) activity. However, one mutation, c.4127dupC, escaped nonsense-mediated decay. The corresponding mutant protein (p.Arg1377Alafs(*) 27) showed reduced colocalization with fibronectin, leading to an abnormal morphology of microfibrils in fibroblast cultures, while retaining normal TGFβ activity. We conclude that LTBP4 mutations cause disease through both loss of function and gain of function mechanisms

Meat quality of lambs fed different saltbush hay (Atriplex nummularia) levels

Climate changes have increased soil and water salinity, compromising animal produc- tion especially in dry areas where scientists have become more interested in halophyte plants, like saltbush. The effects of saltbush hay levels (30, 40, 50 and 60%) were evaluated based on physical-chemical, nutritional and sensory parameters of Santa Ine^s lamb meat. Thirty-two 8-month-old castrated Santa Ine^s lambs, with initial weights of 22±1.97 kg were used; they were slaughtered after 60 days in the feedlot. The pH, colour, moisture, protein and cholesterol contents did not differ among treatments. Panelists observed a greater inten- sity of lamb smell and flavour (P=0.0035) in the meat of animals that received more con- centrate in the diet. An increase in the inclu- sion of saltbush increased ash percentage (P=0.0232), total saturated (P=0.0035) and polyunsaturated (P=0.0287) fatty acids and reduced the lipids (P=0.0055) and the n-6:n-3 ratio (P=0.0058) of the meat. Therefore, salt- bush hay can be used as a feeding resource in regions with problems of water and soil salini- ty because it does not impair the physical- chemical, nutritional and sensory quality of sheep meat

Bird community composition of two riparian forests at Jacaré-Pepira river, São Paulo, Brazil

A quali-quantitative survey was carried out in two riparian forests fragmente (approximately 40 ha each) at Jacaré-Pepira river. Our intention was to characterize its bird community conceming richness, abundance and species occurrence in these areas. The qualitative survey showed 130 species at Santa Elisa (Brotas, São Paulo) and 151 at Morro Chato (Dourado, São Paulo), whereas the quantitative survey revealed the presence of 69 and 75 species at Santa Elisa and Morro Chato, respectively. The small size and the isolation might be responsible for the low number of species found. Observing the abundance index values (IPA) we realize that there are a few number of species with a high IPA on the one hand, while on the other there is a large number of species with intermediate and low IPA rates. A high detection coefficient (vocalization), low predatoiy rates and competition might have contributed for the higher abundance values found among these species. Furthermore, we have also registered species which are abundant in a fragment, but absent in the other, which might be explained by initial exclusion or local extinction. In spite of the riparian forests being protected by law, its clearing process has not stopped yet. Therefore, the study of this bird community is of uppermost impoitance for the elaboration of both conservation and management projects regarding these areas.10871098Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Experimental characterization of combustion in recirculation zone of double-stage swirl chamber

Paper presented at the 9th International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, Malta, 16-18 July, 2012.The focus of the present work is a new Low-NOx combustor configuration to especial application in gas turbine. The combustion happens in two phases; the first one with oxidant deficiency, or fuel rich combustion, and the second one is a fuel lean combustion. This combustion structure allows the conciliation of low NOx emissions and partial oxidation combustion products, as carbon monoxide and unburned hydrocarbons. In the new concept proposed here, these unfavorable combustion conditions for NOx formation are reached through the dynamic control of reactants mixing process into the chamber. However, the success of this strategy depends on the formation of a strong recirculation zone in the secondary chamber and quick-mixing between air, reminiscent fuel and combustion products. So that the present work shows experimental results about the structure of the recirculation zone using Particle Image Velocimetry (PIV) and the combustion dynamics using Planar Laser Inducing Fluorescence (PLIF). Both techniques were applied in the secondary zone of combustion. The conclusion based on the results presented in this paper can be summarized according to the increase of the recirculation zone intensity: 1. the volume occupied by recirculation zone is greater, the transition to reverse flow is more abrupt and the magnitude of the reverse velocity is higher; 2. intensify the vortices formation; 3. the combustion reactions take place in the central region of recirculation zone.dc201