Antibody dynamics and spontaneous viral clearance in patients with acute hepatitis C infection in Rio de Janeiro, Brazil

Submitted by Sandra Infurna ([email protected]) on 2017-01-18T10:31:05Z No. of bitstreams: 1 clara_yoshida_etal_IOC_2011.pdf: 146042 bytes, checksum: 8ec889224534b76d755828a99d0660c2 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2017-01-18T10:59:52Z (GMT) No. of bitstreams: 1 clara_yoshida_etal_IOC_2011.pdf: 146042 bytes, checksum: 8ec889224534b76d755828a99d0660c2 (MD5)Made available in DSpace on 2017-01-18T10:59:52Z (GMT). No. of bitstreams: 1 clara_yoshida_etal_IOC_2011.pdf: 146042 bytes, checksum: 8ec889224534b76d755828a99d0660c2 (MD5) Previous issue date: 2011Innsbruck Medical University. Department of Medical Statistics, Informatics and Health Economics. Innsbruck, Austria.Harvard Medical School. Boston, MA, USA / Massachussets General Hospital. Division of Infectious Diseases. Boston, MA, USA.Harvard Medical School. Boston, MA, USA / Massachussets General Hospital. Gastrointestinal Unit. Boston, MA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Laboratório Central de Saúde Pública Noel Nutels. Divisão de Hepatites. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil / Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gaffrée Guinle. Unidade de Hepatologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Universitätsklinikum Eppendorf. Medizinische Klinik I. Hamburg, Germany.Fondation Merieux. Emerging Pathogens Laboratory. Lyon, France.University of Innsbruck. Institute of Statistics. Innsbruck, Austria.National Institute on Aging. Gerontology Research Center. Baltimore, USA.Innsbruck Medical University. Department of Medical Statistics, Informatics and Health Economics. Innsbruck, Austria.Innsbruck Medical University. Department of Medical Statistics, Informatics and Health Economics. Innsbruck, Austria.Innsbruck Medical University. Department of Medical Statistics, Informatics and Health Economics. Innsbruck, Austria.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Background: The anti-HCV antibody response has not been well characterized during the early phase of HCV infection and little is known about its relationship to the clinical course during this period. Methods: We analyzed serial anti-HCV antibodies longitudinally obtained from a prospective cohort of 65 patients with acute HCV infection by using a microparticle enzyme immunoassay AxSYM HCV 3.0 (Abbott Diagnostics) during the first 12 months from HCV acquisition in Rio de Janeiro, Brazil. Spontaneous viral clearance (SVC) was defined as undetectable HCV RNA in serum, in the absence of treatment, for three consecutive HCV PCR tests within 12-months of follow-up. Results: Baseline antibody values were similar among patient groups with self-limiting HCV evolution (n = 34) and persistent viremia (n = 31) [median (interquartile range) signal/cut-off ratio (s/co) 78.7 (60.7-93.8) vs. 93.9 (67.8- 111.9), p = 0.26]. During 12-months follow-up, patients with acute spontaneous resolving HCV infection showed significantly lower serial antibody response in comparison to individuals progressing to chronic infection [median (interquartile range) s/co 62.7 (35.2-85.0) vs. 98.4 (70.4-127.4), p < 0.0001]. In addition, patients with self-limiting HCV evolution exhibited an expeditious, sharp decline of serial antibody values after SVC in comparison to those measured before SVC [median (interquartile range) s/co 56.0 (25.4-79.3) vs. 79.4 (66.3-103.0), p < 0.0001]. Conclusion: Our findings indicate a rapid short-term decline of antibody values in patients with acute spontaneous resolving HCV infection

Gender and partnership differences in the context of economic recession

BACKGROUND: The economic recession produced a rapid rise of unemployment rates that was more visible in Southern European countries. There is evidence that unemployment correlates highly with individuals' poor life satisfaction. OBJECTIVE: To analyse the relationship between life satisfaction, household composition and socioeconomic deprivation in people facing unemployment during the economic recession. METHODS: A sample of 748 unemployed people from Lisbon (Portugal) completed a socio-demographic questionnaire, the Cantril's ladder of life scale, and the latent and manifest benefits of work scale (LAMB). Multiple regression analyses were used to test the associations between life satisfaction and all other variables. RESULTS: Partnered people report higher life satisfaction compared to singles. Financial deprivation and lack of structured time were the strongest factors negatively related to life satisfaction in both partnered and single people. Having children had a particular negative effect on the life satisfaction of partnered men; and living with an unemployed partner together with lack of social contact and high enforced activity had a negative effect on life satisfaction in partnered women. CONCLUSION: The heterogeneity of socioeconomic needs found by household composition bring practical policy implications for support actions targeting unemployed individuals in the unique context of economic recession.publishersversionpublishe

Synthesis of 1,2,4 trioxolanes from vegetable oils for pharmaceutical and veterinary application

<p><em>Vegetable oils</em><em> are rich in mono-and poly-unsaturated fatty acids, and these compounds contain carbon-carbon double bonds available for chemical/structural modification, especially via oxidative processes. Sunflower oil obtained from seed of sunflower (</em><em>Helianthus annuus) contains a large amount of these acids, such as linoleic (48-74%) and oleic (14-39%) acids, proving to be an ideal starting material for these changes [1]. Ozone is an oxidizing agent that reacts with double bonds of fatty acids present in vegetable oils to form ozonides or 1,2,4 trioxolanes,  and peroxidic species such as hydroperoxides, hydrogen peroxide, polymeric peroxides and other organic peroxides. These compounds are of great pharmaceutical interest for the treatment of various dermatological diseases, due to their antimicrobial properties and stimulating action on tissue repair and regeneration [2]. In this work, the sunflower oil was ozonized, and the ideal conditions, as reaction time, ozone concentration and temperature, were determined. The IR and ¹H and ¹³C NMR of ozonized oils confirm the formation of 1,2,4 trioxolane ring according to the mechanism proposed by Criegee [3]. The ozonolysis reaction was performed under different conditions and the product is in the process of knowledge protection or patent</em><em>.</em></p

Multiple cerebral aneurysms originating from previously resected cardiac myxoma

Primary cardiac tumors are very rare, accounting for 0,001 – 0,03% in autopsy series, cardiac myxoma (CM) account for approximately 50% of them. Association between CM and cerebral aneurism is well documented in this condition but the occurrence of cerebral aneurism after resection of CM is very rare there is only 40 reports in medical literature. We present a case of 45 years old female patient with multiple cerebral aneurysms 2 years after a successful resection of cardiac myxoma, submitted to radiosurgery for 2 bigger aneurisms with good result. This report ads to literature new clinical findings and a hypothesis of radiosurgery as an effective option of treatment for cerebral aneurisms with origin in CM.Primary cardiac tumors are very rare, accounting for 0,001 – 0,03% in autopsy series, cardiac myxoma (CM) account for approximately 50% of them. Association between CM and cerebral aneurysm is well documented in this condition but the occurrence of cerebral aneurysm after resection of CM is very rare, with only 40 reports in medical literature. We present a case of a 45 years old female patient with multiple cerebral aneurysms 2 years after a successful resection of cardiac myxoma, submitted to radiosurgery for 2 bigger aneurysms with good results. This report ads to literature new clinical findings and a hypothesis of radiosurgery as an effective option of treatment for cerebral aneurysms with origin in CM. Keywords: Cardiac Myxoma; Cerebral aneurysm; Radiosurgery; Neurosurgery.Keywords: Cardiac Myxoma; Cerebral aneurism; Radiosurgery; Neurosurgery

A Cross Section Study to Determine the Prevalence of Antibodies against HIV Infection among Hepatitis B and C Infected Individuals

Submitted by Sandra Infurna ([email protected]) on 2016-12-01T10:06:28Z No. of bitstreams: 1 geane_flores_etal_IOC_2016.pdf: 295930 bytes, checksum: ed873280bd363d5f854cf8ea55438996 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2016-12-01T10:15:09Z (GMT) No. of bitstreams: 1 geane_flores_etal_IOC_2016.pdf: 295930 bytes, checksum: ed873280bd363d5f854cf8ea55438996 (MD5)Made available in DSpace on 2016-12-01T10:15:09Z (GMT). No. of bitstreams: 1 geane_flores_etal_IOC_2016.pdf: 295930 bytes, checksum: ed873280bd363d5f854cf8ea55438996 (MD5) Previous issue date: 2016Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.(1) BACKGROUND: There are limited data regarding human immunodeficiency virus (HIV) prevalence among hepatitis B virus (HBV) or hepatitis C virus (HCV) infected individuals. The aim of this cross-sectional study is to determine the prevalence of HBV and HCV infection among HIV individuals; (2) METHODS: A total of 409 patients (126 HBV+ and 283 HCV+) referred to the Brazilian Reference Laboratory for Viral Hepatitis from 2010 to 2013 donated serum samples. Anti-HIV, HBsAg, anti-HBc, anti-HBs, anti-HBcIgM, anti-HBe, HBeAg, and anti-HCV antibodies were measured, and anti-HCV positive samples were tested for viral RNA and genotype; (3) RESULTS: The anti-HIV antibody prevalence was 10.31% and 4.59% among HBV+ and HCV+ patients, respectively. The HCV mean (SD) viral load was log 5.14 ± 1.64 IU/mL, and genotype I was most prevalent (163/283). Anti-HBs and anti-HBc were detected in 40% and 26% of HCV+ individuals, respectively. Among the HBV+ population, the presence of anti-HIV antibodies was associated with male gender, marital status (married), tattoo, sexual orientation, sexual practices (oral sex and anal sex), history of sexually transmitted diseases (STDs), history of viral hepatitis treatment, and a sexual partner with hepatitis or HIV. For the HCV+ group, the presence of anti-HIV antibodies was associated with female gender, marital status (married), anal intercourse, previous history of STDs, and number of sexual partners; (4) CONCLUSION: A high prevalence of anti-HIV antibodies was found among individuals with HBV and HCV, showing the importance of education programmes towards HIV infection among HBV- and HCV-infected individuals