A polymerase III-like reinitiation mechanism is operating in regulation of histone expression in archaea

An archaeal histone gene from the hyperthermophile Pyrococcus furiosus containing four consecutive putative oligo-dT terminator sequences was used as a model system to investigate termination signals and the mechanism of termination in vitro. The archaeal RNA polymerase terminated with high efficiency at the first terminator at 90°C when it contained five to six T residues, at 80°C readthrough was significantly increased. A putative hairpin structure upstream of the first terminator had no effect on termination efficiency. Template competition experiments starting with RNA polymerase molecules engaged in ternary complexes revealed recycling of RNA polymerase from the terminator to the promoter of the same template. This facilitated reinitiation was dependent upon the presence of a terminator sequence suggest-ing that pausing at the terminator is required for recycling as in the RNA polymerase III system. Replacement of the sequences immediately down-stream of the oligo-dT terminator by an AT-rich segment improved termination efficiency. Both AT-rich and GC-rich downstream sequences seemed to impair the facilitated reinitiation pathway. Our data suggest that recycling is dependent on a subtle interplay of pausing of RNA polymerase at the ter-minator and RNA polymerase translocation beyond the oligo-dT termination signal that is dramatically affected by downstream sequences

The ExaVolt Antenna: A Large-Aperture, Balloon-embedded Antenna for Ultra-high Energy Particle Detection

We describe the scientific motivation, experimental basis, design methodology, and simulated performance of the ExaVolt Antenna (EVA) mission, and planned ultra-high energy (UHE) particle observatory under development for NASA's suborbital super-pressure balloon program in Antarctica. EVA will improve over ANITA's integrated totals - the current state-of-the-art in UHE suborbital payloads - by 1-2 orders of magnitude in a single flight. The design is based on a novel application of toroidal reflector optics which utilizes a super-pressure balloon surface, along with a feed-array mounted on an inner membrane, to create an ultra-large radio antenna system with a synoptic view of the Antarctic ice sheet below it. Radio impulses arise via the Askaryan effect when UHE neutrinos interact within the ice, or via geosynchrotron emission when UHE cosmic rays interact in the atmosphere above the continent. EVA's instantaneous antenna aperture is estimated to be several hundred square meters for detection of these events within a 150-600 MHz band. For standard cosmogenic UHE neutrino models, EVA should detect of order 30 events per flight in the EeV energy regime. For UHE cosmic rays, of order 15,000 geosynchrotron events would be detected in total, several hundred above 10 EeV, and of order 60 above the GZK cutoff energyComment: 20 pages, 14 figures; introductory section shortened; additional horizontal polarization simulation results included. In final review for Astroparticle Physic

Heavy quarkonium: progress, puzzles, and opportunities

A golden age for heavy quarkonium physics dawned a decade ago, initiated by the confluence of exciting advances in quantum chromodynamics (QCD) and an explosion of related experimental activity. The early years of this period were chronicled in the Quarkonium Working Group (QWG) CERN Yellow Report (YR) in 2004, which presented a comprehensive review of the status of the field at that time and provided specific recommendations for further progress. However, the broad spectrum of subsequent breakthroughs, surprises, and continuing puzzles could only be partially anticipated. Since the release of the YR, the BESII program concluded only to give birth to BESIII; the $B$-factories and CLEO-c flourished; quarkonium production and polarization measurements at HERA and the Tevatron matured; and heavy-ion collisions at RHIC have opened a window on the deconfinement regime. All these experiments leave legacies of quality, precision, and unsolved mysteries for quarkonium physics, and therefore beg for continuing investigations. The plethora of newly-found quarkonium-like states unleashed a flood of theoretical investigations into new forms of matter such as quark-gluon hybrids, mesonic molecules, and tetraquarks. Measurements of the spectroscopy, decays, production, and in-medium behavior of c\bar{c}, b\bar{b}, and b\bar{c} bound states have been shown to validate some theoretical approaches to QCD and highlight lack of quantitative success for others. The intriguing details of quarkonium suppression in heavy-ion collisions that have emerged from RHIC have elevated the importance of separating hot- and cold-nuclear-matter effects in quark-gluon plasma studies. This review systematically addresses all these matters and concludes by prioritizing directions for ongoing and future efforts.Comment: 182 pages, 112 figures. Editors: N. Brambilla, S. Eidelman, B. K. Heltsley, R. Vogt. Section Coordinators: G. T. Bodwin, E. Eichten, A. D. Frawley, A. B. Meyer, R. E. Mitchell, V. Papadimitriou, P. Petreczky, A. A. Petrov, P. Robbe, A. Vair