Partner Capabilities and Alliance Time Frame: An Analysis of International Strategic Alliances from the CEE

Partner selection is one of the most discussed issues in strategic alliances literature. However, the majority of research has typically focused on generic partner characteristics and presented conceptual models for alliance partner selection, addressing clan image but only limited pieces of the partner selection puzzle. Rooted in the resource-based view, this paper suggests that partner selection is contingent upon the intended time frame of strategic alliances and presents a new and intensive conceptual framework that examines the appropriate partner capability for strategic alliances, in the case of short/medium-term alliances and long-term ones. Based on empirical evidences from 736 alliances in the CEE region, the findings stress the differences between varied partner capabilities in short/medium-term and long-term alliances. Accordingly, the significance of technological capability increases with the number of year’s alliances endured. Moreover, the importance of market capability decreases significantly when alliances last for a longer time frame

Biochemical and immunological characterization of Toxoplasma gondii macrophage migration inhibitory factor

Macrophage migration inhibitory factor (MIF) is a proinflammatory molecule in mammals that, unusually for a cytokine, exhibits tautomerase and oxidoreductase enzymatic activities. Homologues of this well conserved protein are found within diverse phyla including a number of parasitic organisms. Herein, we produced recombinant histidine-tagged Toxoplasma gondii MIF (TgMIF), a 12-kDa protein that lacks oxidoreductase activity but exhibits tautomerase activity with a specific activity of 19.3 μmol/min/mg that cannot be inhibited by the human MIF inhibitor ISO-1. The crystal structure of the TgMIF homotrimer has been determined to 1.82 Å, and although it has close structural homology with mammalian MIFs, it has critical differences in the tautomerase active site that account for the different inhibitor sensitivity. We also demonstrate that TgMIF can elicit IL-8 production from human peripheral blood mononuclear cells while also activating ERK MAPK pathways in murine bone marrow-derived macrophages. TgMIF may therefore play an immunomodulatory role during T. gondii infection in mammals