A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Screening of Five and Six-Membered Nitrogen-Containing Heterocyclic Compounds as New Effective Stimulants of Linum Usitatissimum L. Organogenesis in Vitro

Linum usitatissimum L. (flax or linseed) is one of the oldest economically important crops used in biotechnology as a source for bioactive compounds. Flax fiber and seed have industrial significance as a source of cellulosic fiber for textile and paper industry and seed oil for pharmaceutical, cosmetic and food industry. The elaboration of new effective techniques for in vitro regeneration of Linum usitatissimum L. is very actual problem today. The influence of low molecular weight five and sixmembered nitrogen-containing heterocyclic compounds on organogenesis of Linum usitatissimum L. cultivar heavenly in vitro has been studied. It was found that heterocyclic compounds derivatives pyridine, pyrimidine, pyrazole and isoflavones revealed high stimulating effect on direct shoot organogenesis of Linum usitatissimum L. in vitro. The maximal shoot regeneration frequency was obtained on the MS media containing heterocyclic compounds at the concentration 10-8 M/l as compared to lower regeneration frequency obtained on the control MS medium containing 1 mg/l BAP and 0.05 mg/l NAA. The maximal index of explants with regeneration (in %) that exceeded almost twice the same index in control MS medium was obtained on the MS medium containing heterocyclic compound derivative of isoflavones. At the same time, the index of explants with regeneration (in %) that exceeded one and a half times the same index in control MS medium and the maximal index of shoots elongated more than 1 cm (in %) that exceeded almost twice the same index in control MS medium were obtained on the MS media containing heterocyclic compounds derivatives of pyridine, pyrimidine and pyrazole. The current study confirms perspective of using of low molecular weight five and six-membered nitrogen-containing heterocyclic compounds at the concentration 10-8 М/l of the MS medium as new effective substitutes of traditional growth regulators auxin NAA and cytokinin BAP for regeneration of Linum usitatissimum L. cultivar heavenly in vitro

Induced polyploidization in Brassica campestris L. (Brassicaceae)

Present experimental design has been made up to obtain crop with higher ploidy level via synthetic polyploidization. Since ploidy manipulation is generally associated with the obtainment of some increased enviable traits of the crop and also provides them greater adaptability to unfavorable or harsh circumstances as compared to its diploids counterparts. Thus, herein present research autotetraploids of Brassica campestris L. have been lucratively achieved by the application of colchicine. Two methods of treatment were utilized i.e. seed treatment and seedling treatment. No polyploidy could be obtained through seed treatment while seedling treatment responded well towards polyploidy. However, the status of autotetraploidy has been confirmed by cytomorphological investigations of treated plants as against its diploids counterparts. For the purpose, morphological parameters such as increased stomata size, pollen diameter, flower size, reproductive organs whereas reduction in plant height, leaf length, leaf breadth, stomata frequency, number of flowers/inflorescence etc. were appraised. Further, cytological observations were made that had clearly revealed the doubling of genome in the autotetraploids as compared to diploids. Meanwhile, pollen fertility and size of pollen grains were evaluated as well.Разработана экспериментальная схема получения сельскохозяйственных культур с более высокой плодиностью путем искусственной полиплоидизации. Изменение плоидности обычно связано с приобретением некоторых важных признаков у таких культур, а также обеспечивает их более высокую приспособляемость к неблагоприятным или суровым условиям внешней среды по сравнению с диплоидными аналогами. В настоящем исследовании представлено успешное получение автотетраплоидов Brassica campestris L. с помощью колхицина. Для этого применяли два метода обработки – семян и проростков. Никакой полиплоидии не обнаружено при обработке семян, тогда как после обработки проростков полиплоиды были получены. С помощью цитоморфологических исследований подтверждено наличие автотетраплоидности у обработанных растений по сравнению с их диплоидными аналогами. Для этого оценивали такие морфологические параметры, как увеличенные размер устьиц, диаметр пыльцы, размер цветка, репродуктивные органы, а также уменьшенные высота растений, длина листа, ширина листа, частота устьиц, число цветков и соцветий. Более того, в результате цитологических исследований четко установлено удвоение генома у автотетраплоидов по сравнению с диплоидами. Наряду с этим в работе оценивали фертильность пыльцы и величину пыльцевых зерен

Fitness costs linked to dinitroaniline resistance mutation in Setaria

A mutant Thr-239-Ileu at the α2-tubulin gene was found to confer resistance to dinitroanilines, a family of mitosis-disrupting herbicides. However, mutations affecting microtubule polymerization and cell division are expected to impact growth and reproduction, that is, the fitness of a resistant weed or the yield of a tolerant crop, although it has not been demonstrated yet. This study was designed to test this hypothesis for the growth and reproduction of near-isogenic resistant and susceptible materials that were created in F2 and F3 generations after a Setaria viridis x S. italica cross. Differential growth was noticeable at the very onset of seedling growth. The homozygous resistant plants, grown both in a greenhouse cabinet and in the field, were smaller and had lower 1000-grain weight and therefore a lower yield. This fitness penalty is certainly due to modified cell division kinetics. Although the presence of the mutant allele accounted for 20% yield losses, there were also measurable benefits of dinitroaniline resistance, and these benefits are discussed