Quality Improvement of Disqualified Tiger Prawn (Penaeus Monodon Fab.) Broodstock Using Dopamine

Disqualified tiger prawn broodstock is the one that has undergone ablation after two breeding periodes, which causes high production cost, pollution of environment and difficulties in providing it continuously with enough quantity at exact time. The purpose of this study is to find the proper dosage of dopamine which will give good quality for breeding of the disqualified tiger prawn, because dopamine inhibits maturation, so it has enough time for maturation and can be improved the eggs quality. This experiment used 15 pairs the twice breeding tiger prawn, which is taken from Perigi (Central Java). The sample were treated with difference dosage of dopamine (dosage 10-9 , 10-8, 10-7, 10-6 moll and control/injected by aquabidest). The parameters were identified in this experiment are duration (days) for eggs maturation, fecundity, fertilizing rate, hatching rate, egg diameter and phototaxis respnnsive of the nauplii. The result of this research are the of the average of duration for eggs maturation on the broodstock that were injected dopamine, dosage 10-9 , 10-8, 10-7, 10-6 moll and control , were 14, 12, 4, 2, 5 days. Fecundity 561.489, 503.000, 384.205, 230.850 and 150.034. Fertilizing rate 89,8 %, 82,1 %, 56.0 %, 46,3 %, and 23,2 %. Hatching rate 79,53%, 72,53%, 47,75%, 12,27%, and 36.6% . Diameter 0,29; 0,29; 0,29; 0,27; and 0,27mm. The nauplii that had produced by injected broodstock by dosage 10-9 , 10-8, 10-7, have the positive phototaxis responsive while dosage 10-6 and control were weak

Pematangan Gonad Ikan Sidat Betina (Anguilla Bicolor Bicolor) melalui Induksi Ekstrak Hipofisis

Tujuan penelitian ini adalah untuk menjelaskan pengaruh pemberian ekstrak hipofisis ikan mas terhadap pematangan gonad ikan sidat tropis betina (Anguilla bicolor bicolor). Ikan sidat dengan bobot 600 gram telah digunakan sebagai materi biologis. Ekstrak hipofisis ikan mas dengan tingkat 0, ¼ dan ½ dosis dan tiga tipe frekuensi penyuntikan yakni setiap 1, 2 dan 3 minggu dijadikan sebagai perlakuan. Parameter yang diamati adalah Gonado somatic index (GSI), Hepatosomatic index (HSI), diameter folikel, kadar lemak dalam folikel, serta kadar glikokonjugat dalam folikel dan hati. Hasil percobaan menunjukkan bahwa penyuntikan ekstrak hipofisis ikan mas dengan ½ dosis yang dilakukan setiap minggu selama 10 minggu memberikan pengaruh yang terbaik

Spermatogenesis and Semen Quality of Male Muntjak (Muntiacus Muntjak Muntjak) During Antler Growth Periods

Muntjak (Muntiacus muntjak muntjak) belongs to Cervidae family which distributed in Java Island and Southern part of Sumatera. This cervid has been protected by Indonesian Government since 1999. In order to support breeding program of the species and to avoid them from extinction, itsreproductive biology such as spermatogenesis and the correlation to semen quality is important to be investigated. Therefore, the objective of this study was to examine spermatogenesis and semen quality of two adult male muntjaks during antler growth periods that consist of hard antler (H), casting (C), andvelvet antler (V). Testicular tissues and semen (ejaculates) were obtained by core needle biopsy and electroejaculation methods respectively. Testicular tissues were processed histologically and stained with periodic acid Schiff (PAS) to observe spermatogenesis whereas semen was evaluated to obtaine itsquality. The results showed that spermatogenic activities were detected in all antler periods which marked by PAS positive staining (magenta colour) of round and elongated spermatid acrosomes. In H period, spermatogenic activity was higher than those C and V periods. According to semen evaluation,motile spermatozoa were found with different concentration in all antler periods. The highest sperm concentration (x 106 spermatozoa/ml) in both of muntjaks was found in H period (506.25 ± 61.87), and slightly decreased in C (288.75 ± 37.12), and V periods (362.60 ± 17.68). These finding showed thatspermatogenesis to produce spermatozoa is taken place while muntjaks are in C and V periods with differ activities that provable with the existence of motile spermatozoa from ejaculates in both of male muntjaks. Therefore, muntjaks could provide reproductive function throughout the year of reproductiveaseasonality which is similar to the reeves and formosan muntjak

PAT-1 Case report: Metastatic ganglioneuroma in a Sumatran Tiger

Neuroblastic tumors, which are uncommon tumors of nerve cell origin, are classified into neuroblastoma, ganglioneuroblastoma, and ganglioneuroma depend-ing on the degree of maturation of neoplastic neuroblasts and development of schwannian stroma [2,4,7].  Ganglioneuroma is a rare neuroectodermal tumor of the peripheral nervous system. Ganglio-neuromas generally are considered to be benign neoplasms composed of both mature ganglion cells and nerve fascicles with axons, Schwann cells, fibroblasts, and other connective tissue elements [1].  The aim of this study is to clarify the pathomor-phological feature of a subcutaneous ganglio-neuroma which occurred in a Sumatran tiger.

PENGOPTIMALAN KINERJA MOTORIK PADA PENUAAN FISIOLOGIS DAN PENUAAN AKIBAT STRES OKSIDATIF DENGAN ALANIN-GLUTAMIN DIPEPTIDA DAN HUBUNGANNYA DENGAN PERBAIKAN FUNGSI HIPOKAMPUS

Penelitian ini bertujuan membuktikan pengaruh alanin-glutamin dipeptida 7% terhadap perbaikan fungsi hipokampus melalui pengamatan beberapa parameter kinerja motorik seperti jarak tempuh (JT), waktu stereotipe (WT), waktu ambulatori (WA), dan waktu istirahat (WI). Penelitian ini dilakukan dengan memberikan alanin-glutamin dipeptida 7% selama 12 hari pada tikus umur 12 dan 24 bulan yang mengalami penuaan fisiologis dan penuaan akibat stres oksidatif. Pengamatan terhadap semua parameter kinerja motorik dilakukan dengan alat Opto-Varimex yang dikoneksikan dengan software Autotract. Hasil penelitian menunjukkan alanin-glutamin dipeptida 7% berpengaruh terhadap perbaikan kinerja motorik pada kondisi penuaan fisiologis dan penuaan akibat stres oksidatif

Morphological Study of the Oesophagus and Stomach of the Cave Swiflets (Collocalia linchi)

This study was conducted with aim to tend out morphology of oesophagus and stomach of the cave swiflets (Collocalia linchi) at macroscopic and microscopic levels.  The data revealed that esophagus was 2.93 cm in length and possessed no crop.  The stomach was small and the isthmus was not clear. Mucosa of the esophagus was lined by stratified squoamus epithelium. Esophageal gland was mucous type. The glands were well developed and distributed along the esophagus. The external muscle layer consisted of inner circular and outer longitudinal layers.  The stomach could be distinguished into proventriculus and ventriculus with no clear isthmus between them. The mucosa of proventriculus was lined by single columnar epithelium. The ventricular gland area was divided into cardiac, fundic  and pyloric gland areas. The surface of ventriculus was lined with cuticula. In general the esophagus and stomach of the cave swiftlets were simple with no crop in the esophagus and isthmus in the stomach. These might be related with the kind of food and feeding behavior of this species

Granulovacuolar Degeneration in Brains of Senile Cynomolgus Monkeys

Neurons with histopathological changes consistent with granulovacuolar degeneration (GVD) were found in brain sections from aged cynomolgus monkeys (Macaca fascicularis) with clinical and pathological signs of cognitive aging. To our knowledge, this is the first reported description of GVD in non-human primates. GVD-like lesions were found also in age-matched cognitively healthy subjects, albeit in lower numbers, suggesting that they may relate to aging and the increase may have tendency to increase with the memory deficits. The increased incidence of GVD-like lesions in memory-impaired subjects with pahological backgrounds of senile plaques (SPs) and tauopathy is, however, an interesting observation of relevance to the characterization of pathologies in the spontaneous cynomolgus monkey model of human Alzheimer’s type of brain pathology