Current knowledge on Amaranthus spp.: research avenues for improved nutritional value and yield in leafy amaranths in sub-Saharan Africa. Euphytica

Abstract In the past 20 years, very little progress has been achieved in reducing food insecurity, child malnutrition and hunger in Africa. Under-nutrition and micronutrients deficiencies are widespread and affect mainly women and children. To address these problems, increased consumption of African leafy vegetables is promoted as sources of both micronutrients and bio-active compounds. Widely promoted African leafy vegetables include Amaranthus spp., a taxonomic group cultivated worldwide. Species of this genus are used as pseudo-cereals in Europe and America, and are mostly planted as vegetables in Africa. Amaranthus has been rediscovered as a promising food crop mainly due to its resistance to heat, drought, diseases and pests, and the high nutritional value of both seeds and leaves. Leaves are rich in proteins and micronutrients such as iron, calcium, zinc, vitamin C and vitamin A. All parts of the plant are used as medicine to heal many diseases in African communities. This paper focuses on leafy amaranths traditionally utilized on the continent. It briefly reviews the current knowledge on taxonomy, ecology, nutritional and nutraceutical value, production and cultivation systems, reproductive biology, genetic resources and breeding of amaranths. Species of interest include: A. blitum, A. caudatus, A. cruentus, A. dubius, A. hypochondriacus, A. spinosus, A. thunbergii, A. tricolor, and A. viridis. Research and development opportunities on nutritive and nutraceutical properties, production and commercialization, taxonomic evaluation and breeding perspectives were explored

Designing User-Friendly Grids for the Gender-Specification of Rural, Semi-Urban, and Urban School Toilets in West Africa: Lessons from the Republic of Benin

Gender equity, quality education, and sanitation in rural and urban areas are widely considered as development imperatives. However, while many countries of the world are already far with the gendering of their schools’ toilets, most others still tend to systematically construct the same number of toilet cabins for female and male students, often ignoring specific needs associated with biophysical differences and disabilities among the students. This poor gendering of school toilets prevailed in the Republic of Benin until 2013, when, within the framework of the Pluriannual Water and Sanitation Programme (PPEA phase 2) funded by the Netherlands’ Ministry of Cooperation through its Embassy in the Republic of Benin, the Directorate of Hygiene and Sanitation of the Ministry of Public Health in collaboration with Water and Sanitation for Africa Agency, decided to launch a study for gender and disability specific toilets in primary and secondary schools. This original paper discusses the process towards the design of norms and grids for the calculation of toilet cabins and urinals needs of primary and secondary school students of rural, semi-urban, urban, Sahelian, Sudanian, lake, and coastal areas of the Republic of Benin. The study took place in four steps. The first step consisted of identifying key factors that affect the use of school toilets by students, especially female and disabled students. The second step consisted of observing and recording the use of school toilets by students, and building on those observations and records to estimate the number of toilet cabins and urinals required per agroecological and sociocultural area. The third step built on the results from the first and second steps to design simplified grids that allow sanitation and schools’ decision-makers to easily and quickly calculate the number of toilet cabins and urinals required for the target schools, per gender and disability. The fourth step consisted of building the capacities of potential users of the grids, for their relevant use. The findings indicate a variation of needs among primary and secondary schools, and the study zones, due to sociocultural and development differences. The grids have been in use in Benin since 2016 and it has easily helped to calculate gender-specific toilet needs in the study areas. After five years of use, their performance requires to be assessed for further adaptations. A further paper will report on the performance of these grids

Designing User-Friendly Grids for the Gender-Specification of Rural, Semi-Urban, and Urban School Toilets in West Africa: Lessons from the Republic of Benin

Gender equity, quality education, and sanitation in rural and urban areas are widely considered as development imperatives. However, while many countries of the world are already far with the gendering of their schools’ toilets, most others still tend to systematically construct the same number of toilet cabins for female and male students, often ignoring specific needs associated with biophysical differences and disabilities among the students. This poor gendering of school toilets prevailed in the Republic of Benin until 2013, when, within the framework of the Pluriannual Water and Sanitation Programme (PPEA phase 2) funded by the Netherlands’ Ministry of Cooperation through its Embassy in the Republic of Benin, the Directorate of Hygiene and Sanitation of the Ministry of Public Health in collaboration with Water and Sanitation for Africa Agency, decided to launch a study for gender and disability specific toilets in primary and secondary schools. This original paper discusses the process towards the design of norms and grids for the calculation of toilet cabins and urinals needs of primary and secondary school students of rural, semi-urban, urban, Sahelian, Sudanian, lake, and coastal areas of the Republic of Benin. The study took place in four steps. The first step consisted of identifying key factors that affect the use of school toilets by students, especially female and disabled students. The second step consisted of observing and recording the use of school toilets by students, and building on those observations and records to estimate the number of toilet cabins and urinals required per agroecological and sociocultural area. The third step built on the results from the first and second steps to design simplified grids that allow sanitation and schools’ decision-makers to easily and quickly calculate the number of toilet cabins and urinals required for the target schools, per gender and disability. The fourth step consisted of building the capacities of potential users of the grids, for their relevant use. The findings indicate a variation of needs among primary and secondary schools, and the study zones, due to sociocultural and development differences. The grids have been in use in Benin since 2016 and it has easily helped to calculate gender-specific toilet needs in the study areas. After five years of use, their performance requires to be assessed for further adaptations. A further paper will report on the performance of these grids

Differential household vulnerability to climatic and non-climatic stressors in semi-arid areas of Mali, West Africa

Semi-Arid Regions (SARs) of West Africa are considered climate change “hotspots” where strong ecological, economic and social impacts converge to make socio-ecological systems particularly vulnerable. While both climatic and non-climatic drivers interact across scales to influence vulnerability, traditionally, this inter-connectedness has received little attention in vulnerability assessments in the region. This study adopted the vulnerability patterns framework, operationalized using the Multidimensional Livelihood Vulnerability approach to include both climatic and nonclimatic stressors to analyze differential household vulnerability in SARs of Mali. Findings showed that while drought was the most mentioned climate-related stressor, households were also exposed to a diversity of environmental and socio-economic stressors, including food scarcity, livestock disease, labour unavailability, crop damage, and erratic rainfall patterns. The typology revealed three vulnerability archetypes differentiated by adaptive capacity and sensitivity. Availability of productive household members, household resource endowments, livelihood diversification and social networks were the main discriminant factors of household adaptive capacity, while challenges relating to food and water security make households more sensitive to stressors. The analysis highlighted the heterogeneity in household vulnerability patterns within and across communities. Failing to account for this heterogeneity in adaptation planning might result in a mismatch between adaptation needs and interventions, and potentially in maladaptation

Morphological characterization of pineapple (Ananas comosus) genetic resources from Benin

International audienceIntroduction - The narrow knowledge of the genetic variability in cultivated pineapple in the Republic of Benin and in other West African countries limits its efficient use and its expansion in the international market; it also hinders the efficient development of pineapple while the crop is listed among the top three priority fruit species to be promoted in Africa. Materials and methods - In this study, we assessed pineapple morphological diversity of 55 accessions collected in Benin. Ten qualitative and twenty quantitative traits were used to describe them. Stepwise discriminant analysis and hierarchical cluster analysis were performed to identify quantitative morphological traits which best differentiate accessions and group them into cultivars/morphotypes. Results and discussion - Five pineapple cultivars were identified and characterized for Benin, including 'Smooth Cayenne', 'Baronne de Rothschild', 'Perola', 'Singapore Spanish', and 'Green Spanish'. We observed significant morphological variation among the cultivars. The collected materials were grouped in three clusters based on flowering date, fruit diameter, fruit shelf life, water content, leaf width, fruit weight and the crown height and weight. Correlation analyses between descriptors revealed positive relationships between fruits weight, peduncle diameter, and conicity index in 'Cayenne' and 'Spanish'. Conclusion - This study showed the existence of clear morphological variation among pineapple cultivars which could be used for fruit improvement through clonal selection and farmer training on propagule production and crop homogeneity. 'Singapore Spanish' and 'Green Spanish' could be promoted for their attractive shell color and long shelf life

Understanding Molecular Mechanisms of Seed Dormancy for Improved Germination in Traditional Leafy Vegetables: An Overview

Loss of seed viability, poor and delayed germination, and inaccessibility to high-quality seeds are key bottlenecks limiting all-year-round production of African traditional leafy vegetables (TLVs). Poor quality seeds are the result of several factors including harvest time, storage, and conservation conditions, and seed dormancy. While other factors can be easily controlled, breaking seed dormancy requires thorough knowledge of the seed intrinsic nature and physiology. Here, we synthesized the scattered knowledge on seed dormancy constraints in TLVs, highlighted seed dormancy regulation factors, and developed a conceptual approach for molecular genetic analysis of seed dormancy in TLVs. Several hormones, proteins, changes in chromatin structures, ribosomes, and quantitative trait loci (QTL) are involved in seed dormancy regulation. However, the bulk of knowledge was based on cereals and Arabidopsis and there is little awareness about seed dormancy facts and mechanisms in TLVs. To successfully decipher seed dormancy in TLVs, we used Gynandropsis gynandra to illustrate possible research avenues and highlighted the potential of this species as a model plant for seed dormancy analysis. This will serve as a guideline to provide prospective producers with high-quality seeds

Agromorphological Characterization Revealed Three Phenotypic Groups in a Region-Wide Germplasm of Fonio (Digitaria exilis (Kippist) Stapf) from West Africa

International audienceFonio is an ancient orphan cereal, cultivated by resource-poor farmers in arid and semi-arid regions of West Africa, who conserved and used the cereal for nutrition and income generation. However, the extent and patterns of phenotypic variation within the fonio germplasm remain scarcely evaluated to inform breeding decisions. In this study, we used alpha lattice design to assess the phenotypic variability of 180 fonio accessions using 20 agro morphological descriptors, including both qualitative and quantitative traits at Bengou research station in 2018 and 2019. Descriptive statistics, combined analysis and multivariate analyses, including principal components analysis (PCA), hierarchical clustering, and multiple factor analysis (MFA) were performed. As results, we found significant differences among fonio accessions and among agro ecological zones of provenance for most of the quantitative traits. Furthermore, highly significant positive correlations were found between grain yield and other yield-related attributes such as harvest index and thousand seeds weight, whereas significant negative correlations were found between grain yield and flowering and maturity times. Clusters analysis revealed three phenotypic groups. Group 1 was characterized by early maturing fonio accessions with higher grain yield. Groups 2 and 3 were characterized by late maturing accessions with intermediate to moderate grain yield. The accessions from Group 1 are candidate for yield improvement and development of fonio lines with enhanced lodging resistance. Accessions from Group 2 and 3 can be improved for yield through marker-assisted selection of best thousand seed weight. This study highlights how traits are correlated within the major phenotypic groups of fonio in West Africa, and we discussed how these groups could be further exploited for improving traits of economic importance. Furthermore, this study exhibited agro morphological descriptors that discriminate fonio accessions and provide useful information for parental selection with economically important agronomic traits

The genome of Gynandropsis gynandra provides insights into whole-genome duplications and the evolution of C4 photosynthesis in Cleomaceae

Gynandropsis gynandra (Cleomaceae) is a cosmopolitan leafy vegetable and medicinal plant, which has also been used as a model to study C4 photosynthesis due to its evolutionary proximity to Arabidopsis. Here, we present a high-quality genome sequence of G. gynandra, anchored onto 17 main super- scaffolds with a total length of 740 Mb, an N50 of 42 Mb and 30,933 well-supported gene models. The G. gynandra genome and previously released genomes of C3 relatives in the Cleomaceae and Brassicaceae make an excellent model for studying the role of genome evolution in the transition from C3 to C4 photosynthesis. We revealed that G. gynandra and its C3 relative Tarenaya hassleriana shared a whole-genome duplication event (Gg-α), then an addition of a third genome (Th-α, +1x) took place in T. hassleriana but not in G. gynandra. Analysis of syntenic copy number of C4 photosynthesis-related gene families indicates that G. gynandra generally retained more duplicated copies of these genes than C3 T. hassleriana, and also that the G. gynandra C4 genes might have been under positive selection pressure. Both whole-genome and single-gene duplication were found to contribute to the expansion of the aforementioned gene families in G. gynandra. Collectively, this study enhances our understanding of the impact of gene duplication and gene retention on the evolution of C4 photosynthesis in Cleomaceae

From shade to light : fonio, an african orphan crop, towards renewed challenges

The FAO recently estimated that demand for food will increase by 70% by 2050. The challenge will not only be on increasing the food supply but also on improving its nutritional value under an accelerating rate of environmental and social changes. One solution would be the wider use of underutilized crops to diversify alimentation and develop sustainable and low-input agriculture. Are orphan crops commodities for the future, and how can they be promoted. In this regard, fonio (Digitaria exilis Stapf) appears to be a promising crop. It is an indigenous staple cereal from Western Africa playing a crucial role in food security. Additionally, it is a model crop in important up-to-date concepts such as resilience, agroecology, population health, climatic changes, poverty reduction, and women's empowerment. Nevertheless, fonio has received limited attention from mainstream research compared to other dryland cereals, such as pearl millet and sorghum. Increasingly available genomic resources promise to promote advanced breeding strategies in fonio. This paper presents the past, present, and future of fonio research. We argue the need for interdisciplinarity and multistakeholder research approaches for increasing fonio production, conservation, and sustainable uses