The association of problematic eating behaviours with food quality and body mass index at 7 years of age

Background/objectives: There is scarce evidence of how certain eating behaviours compromise the compliance with dietary guidelines and weight status in school-aged children. This study aims to evaluate the association of children’s problematic eating behaviours with food quality and body mass index at 7 years of age. Subjects/methods: Participants were children aged 7 years old from a population-based cohort study from Porto, Portugal-Generation XXI. Children’s quantity and speed of ingestion, food refusal at the table and food rewards requesting were evaluated by caregiver’s perception. Food consumption was evaluated by a food frequency questionnaire and compared with age-appropriate guidelines. Children’s weight status was assessed by objective measurements and parent’s perceptions. Associations were estimated by logistic regressions (odds ratio (OR), 95% confidence intervals (95% CI)) adjusted for maternal age, education, smoking during pregnancy, birth type, child’s sex, weight-for-gestational age and sports (n = 3801). Results: Children eating small amounts of food, refusing to eat at the table with the rest of the family during meals and asking for food rewards showed a higher consumption of energy-dense foods (OR = 1.51; 95% CI:1.23–1.86; OR = 1.58; 95% CI:1.16–2.16; OR = 1.56; 95% CI:1.14–2.12) and presented higher odds of consuming fruit and vegetables below recommendations (OR = 1.41; 95% CI:1.22–1.64; OR = 1.20; 95% CI:1.00–1.45; OR = 1.28; 95% CI:1.05–1.55). Children displaying these eating behaviours were less likely to be overweight/obese, hence their parents were less likely to show concern with their weight. Including food quality as covariate in the final models with obesity status did not change the associations. Conclusions: Problematic eating behaviours were associated with poorer food quality (more energy-dense foods and low fruit and vegetables), but food quality does not seem to explain the association with obesity status. Parents were less likely to be concerned about their child’s weight if the child ate slowly or poorly, and accordingly the odds of them being overweight or obese were lower.Generation XXI was funded by the Health Operational Programme—Saúde XXI, Community Support Framework III and the Regional Department of Ministry of Health. This study was supported through FEDER from the Operational Programme Factors of Competitiveness (COMPETE) and through national funding from the Foundation for Science and Technology (FCT; Portuguese Ministry of Education and Science) under the project PTDC/SAU-EPI/121532/2010 (FCOMP-01-0124-FEDER-021177) and through the Investigator Programme (IF/01350/2015–Andreia Oliveira and IF/01060/2015–Ana Cristina Santos). It also had support from the Calouste Gulbenkian Foundation

InfectionCMA: A Cell MicroArray Approach for Efficient Biomarker Screening in In Vitro Infection Assays

The recently emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has forced the scientific community to acquire knowledge in real-time, when total lockdowns and the interruption of flights severely limited access to reagents as the global pandemic became established. This unique reality made researchers aware of the importance of designing efficient in vitro set-ups to evaluate infectious kinetics. Here, we propose a histology-based method to evaluate infection kinetics grounded in cell microarray (CMA) construction, immunocytochemistry and in situ hybridization techniques. We demonstrate that the chip-like organization of the InfectionCMA has several advantages, allowing side-by-side comparisons between diverse cell lines, infection time points, and biomarker expression and cytolocalization evaluation in the same slide. In addition, this methodology has the potential to be easily adapted for drug screening. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Funding text 1: Funding: The Portuguese Foundation for Science and Technology (FCT) funded this project through the Research4COVID19 projects 109_596696487 and RESEARCH COVID-19 projects Ref. 510. FCT also financed the Ph.D. grant to R.J.P. (SFRH/BD/145217/2019) and M.N. (2020.04720.BD). i3S is supported by FEDER–Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020–Operational Program for Competitiveness and Internationalization (POCI), Portugal 2020, and by Portuguese funds through FCT/Ministério da Ciência, Tecnologia e Inovação in the framework of the project ‘Institute for Research and Innovation in Health Sciences’ (POCI-01-0145-FEDER-007274).; Funding text 2: The Portuguese Foundation for Science and Technology (FCT) funded this project through the Research4COVID19 projects 109_596696487 and RESEARCH COVID-19 projects Ref. 510. FCT also financed the Ph.D. grant to R.J.P. (SFRH/BD/145217/2019) and M.N. (2020.04720.BD). i3S is supported by FEDER?Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020?Operational Program for Competitiveness and Internationalization (POCI), Portugal 2020, and by Portuguese funds through FCT/Minist?rio da Ci?ncia, Tecnologia e Inova??o in the framework of the project ?Institute for Research and Innovation in Health Sciences? (POCI-01-0145-FEDER-007274)

Do sound waves transport the AGN energy in the Perseus cluster?

The level of random motions in the intracluster gas lying between 20 and 60 kpc radius in the core of the Perseus cluster has been measured by the Hitomi Soft X-ray Spectrometer (SXS) at 164 ± 10 km s$^{−1}$ . The maximum energy density in turbulent motions on that scale is therefore low. If dissipated as heat, the turbulent energy will be radiated away in less than 80 Myr and cannot spread across the core. A higher velocity is needed to prevent a cooling collapse. Gravity waves are shown to travel too slowly in a radial direction. Here we investigate propagation of energy by sound waves. The energy travels at ~1000 km s$^{−1}$ and can cross the core in a cooling time. We show that the displacement velocity amplitude of the gas required to carry the power is consistent with the Hitomi result and that the inferred density and temperature variations are consistent with $\textit{Chandra}$ observations.ACF, CP, CSR and HRR thank the Hitomi collaboration for the opportunity to participate in the analysis of the SXS data. ACF, CP, HRR and SAW acknowledge support from ERC Advanced Grant FEEDBACK, 340442

Bearing My Heart: The Role of Extracellular Matrix on Cardiac Development, Homeostasis, and Injury Response

The extracellular matrix (ECM) is an essential component of the heart that imparts fundamental cellular processes during organ development and homeostasis. Most cardiovascular diseases involve severe remodeling of the ECM, culminating in the formation of fibrotic tissue that is deleterious to organ function. Treatment schemes effective at managing fibrosis and promoting physiological ECM repair are not yet in reach. Of note, the composition of the cardiac ECM changes significantly in a short period after birth, concurrent with the loss of the regenerative capacity of the heart. This highlights the importance of understanding ECM composition and function headed for the development of more efficient therapies. In this review, we explore the impact of ECM alterations, throughout heart ontogeny and disease, on cardiac cells and debate available approaches to deeper insights on cell–ECM interactions, toward the design of new regenerative therapies.This work was funded by the European Regional Development Fund (ERDF) through COMPETE 2020, Portugal 2020, and by the FCT (Fundação para a Ciência e Tecnologia) (POCI-01-0145-FEDER-030985) and by the FCT/Ministério da Ciência, Tecnologia e Inovação in the framework of individual funding (CEECINST/00091/2018) to DN

Cyclin D 1‐induced proliferation is independent of beta‐catenin in H ead and N eck C ancer

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106775/1/odi12124.pd

Gloeothece sp.—exploiting a new source of antioxidant, anti-inflammatory, and antitumor agents

Bioactive lipidic compounds of microalgae, such as polyunsaturated fatty acids (PUFA) and carotenoids, can avoid or treat oxidation-associated conditions and diseases like inflammation or cancer. This study aimed to assess the bioactive potential of lipidic extracts obtained from Gloeothece sp.–using Generally Recognized as Safe (GRAS) solvents like ethanol, acetone, hexane:isopropanol (3:2) (HI) and ethyl lactate. The bioactive potential of extracts was assessed in terms of antioxidant (ABTS•+, DPPH•,• NO and O2• assays), anti-inflammatory (HRBC membrane stabilization and Cox-2 screening assay), and antitumor capacity (death by TUNEL, and anti-proliferative by BrdU incorporation assay in AGS cancer cells); while its composition was characterized in terms of carotenoids and fatty acids, by HPLC-DAD and GC-FID methods, respectively. Results revealed a chemopreventive potential of the HI extract owing to its ability to: (I) scavenge- NO• radical (IC50, 1258 ± 0.353 µg·mL-1 ); (II) inhibit 50% of COX-2 expression at 130.2 ± 7.4 µg·mL-1; (III) protect 61.6 ± 9.2% of lysosomes from heat damage, and (IV) induce AGS cell death by 4.2-fold and avoid its proliferation up to 40% in a concentration of 23.2 ± 1.9 µg·mL-1 . Hence, Gloeothece sp. extracts, namely HI, were revealed to have the potential to be used for nutraceutical purposes.This research was supported by national funds through FCT—Foundation for Science and Technology within the scope of UIDB/04423/2020, granted to CIIMAR and UIDB/00511/2020 granted to LEPABE funded by national funds through FCT/MCTES (PIDDAC)

Resolved atomic lines reveal outflows in two ultraluminous X-ray sources

Ultraluminous X-ray sources are extragalactic, off-nucleus, point sources in galaxies, and have X-ray luminosities in excess of 3 × 1039 ergs per second. They are thought to be powered by accretion onto a compact object. Possible explanations include accretion onto neutron stars with strong magnetic fields, onto stellar-mass black holes (of up to 20 solar masses) at or in excess of the classical Eddington limit, or onto intermediate-mass black holes (103–105 solar masses). The lack of sufficient energy resolution in previous analyses has prevented an unambiguous identification of any emission or absorption lines in the X-ray band, thereby precluding a detailed analysis of the accretion flow. Here we report the presence of X-ray emission lines arising from highly ionized iron, oxygen and neon with a cumulative significance in excess of five standard deviations, together with blueshifted (about 0.2 times light velocity) absorption lines of similar significance, in the high-resolution X-ray spectra of the ultraluminous X-ray sources NGC 1313 X-1 and NGC 5408 X-1. The blueshifted absorption lines must occur in a fast-outflowing gas, whereas the emission lines originate in slow-moving gas around the source. We conclude that the compact object in each source is surrounded by powerful winds with an outflow velocity of about 0.2 times that of light, as predicted by models of accreting supermassive black holes and hyper-accreting stellar-mass black holes.A.C.F. acknowledges support from the European Research Council through Advanced Grant on Feedback 340492. M.J.M. appreciates support from an STFC advanced fellowship. This work is based on observations with XMM-Newton, an ESA science mission with instruments and contributions directly funded by ESA Member States and NASA. This research has also made use of data obtained from NASA’s Chandra satellite. All codes used are publicly available

An in-depth study of a neutron star accreting at low Eddington rate: On the possibility of a truncated disc and an outflow

Due to observational challenges, our knowledge of low-level accretion flows around neutron stars is limited. We present $\textit{NuSTAR}$, $\textit{Swift}$ and $\textit{Chandra}$ observations of the low-mass X-ray binary IGR J17062-6143, which has been persistently accreting at ≃0.1 per cent of the Eddington limit since 2006. Our simultaneous $\textit{NuSTAR/Swift}$ observations show that the 0.5–79 keV spectrum can be described by a combination of a power law with a photon index of Γ ≃ 2, a blackbody with a temperature of kT$_{bb}$ ≃ 0.5 keV (presumably arising from the neutron star surface) and disc reflection. Modelling the reflection spectrum suggests that the inner accretion disc was located at R$_{in}$ ≳ 100 $\textit{GM/c}$$^{2}$ (≳225 km) from the neutron star. The apparent truncation may be due to evaporation of the inner disc into a radiatively-inefficient accretion flow, or due to the pressure of the neutron star magnetic field. Our $\textit{Chandra }$ gratings data reveal possible narrow emission lines near 1 keV that can be modelled as reflection or collisionally ionized gas, and possible low-energy absorption features that could point to the presence of an outflow. We consider a scenario in which this neutron star has been able to sustain its low accretion rate through magnetic inhibition of the accretion flow, which gives some constraints on its magnetic field strength and spin period. In this configuration, IGR J17062-6143 could exhibit a strong radio jet as well as a (propeller-driven) wind-like outflow.ND is supported by an Netherlands Organisation for Scientific Research (NWO) Vidi grant and an European Commission Marie Curie Intra-European fellowship (contract no.FP-PEOPLE-2013-IEF-627148). CP and ACF are supported by European Research Council (ERC) Advanced Grant Feedback340442. JMM acknowledges support from the Chandra guest observer program. DA acknowledges support from the Royal Society. RW is supported by an NWO Top grant, module 1. This work is based on data from the NuSTAR mission, a project led by California Institute of Technology, managed by the Jet Propulsion Laboratory, and funded by National Aeronautics and Space Administration (NASA).This is the final version of the article. It first appeared from Oxford University Press via https://doi.org/10.1093/mnras/stw235

Human Immunodeficiency Virus (HIV) 2 Superinfection in a Patient Receiving Antiretroviral Therapy With Longstanding HIV-1 Viral Load Suppression

Dual human immunodeficiency virus (HIV) 1 and HIV-2 superinfections are rare but challenging. A HIV-1–infected patient receiving effective antiretroviral therapy was investigated for a severe CD4+ cell count decline. HIV-2 superinfection was diagnosed and genotypic test revealed mutations conferring resistance to most drug class, limiting options for treatment

AGN feedback in the Phoenix cluster

Active galactic nuclei (AGN) release a huge amount of energy into the intracluster medium (ICM) with the consequence of offsetting cooling and star formation (AGN feedback) in the centers of cool core clusters. The Phoenix cluster is among the most massive clusters of galaxies known in the Universe. It hosts a powerful starburst of several hundreds of Solar masses per year and a large amount of molecular gas in the center. In this work we use the high-resolution Reflection Grating Spectrometer (RGS) on board XMM-Newton to study the X-ray emitting cool gas in the Phoenix cluster and heating-cooling balance. We detect for the first time evidence of O VIII and Fe XXI-XXII emission lines, the latter demonstrating the presence of gas below 2 keV. We find a cooling rate of 350 (-200,+250) Msun/year below 2 keV (at the 90% confidence level), which is consistent with the star formation rate in this object. This cooling rate is high enough to produce the molecular gas found in the filaments via instabilities during the buoyant rising time. The line broadening indicates that the turbulence (~ 300 km/s or less) is below the level required to produce and propagate the heat throughout the cool core. This provides a natural explanation to the coexistence of large amounts of cool gas, star formation and a powerful AGN in the core. The AGN activity may be either at a young stage or in a different feedback mode, due to a high accretion rate