Trauma, PTSD, and Traumatic Grief among Palestinian Children

Aim: The aim of this study was to find the relationship between war traumatic experiences due to war on Gaza, PTSD, and traumatic grief in Palestinian children. Methods: The sample included randomly selected 374 children aged 6-16 years. Children completed measures of experience of traumatic events (Gaza Traumatic Checklist), Child Post Traumatic Stress Reaction Index, and Traumatic Grief inventory. Results: Palestinians children experiences variety of traumatic events. No sex differences in reporting trauma. Mean traumatic events reported by children was 12.80 traumatic events. The study showed that 9.3% of the participants lost someone during the war. Mean traumatic grief in boys was 19.96 and 18.29 in girls. For PTSD, 1.3% of children showed no PTSD, 7.2% reported mild PTSD reactions, 29.9% showed moderate PTSD reactions, and 61.5% showed severe to very severe PTSD reactions. Trauma exposure was significantly associated with PTSD. No sex differences in PTSD. Conclusions: This study revealed that children living in area of conflict and war are at risk of developing mental health problems. Study showed that children with traumatic grief need psychosocial support from families and community to enable them of passing through their grief. Moreover, parents have to be involved in all activities given to their children and to be part of such activities to enable them better communication with their children and being able of detecting children with pathological grief and enable them of helping children in overcoming the effect of grief and trauma.We are grateful to the team who collected the data under enormous difficulties. Also, our many thanks to families and children who participated in this study, for their openness in sharing such difficult issues. Also to Mr. Khalil Megdad for his work as statistician in this study

MYO9B polymorphisms in multiple sclerosis

"Single-nucleotide polymorphisms (SNPs) in the 30 region of myosin IXB (MYO9B) gene have recently been reported to associate with different inflammatory or autoimmune diseases. We monitored for the association of MYO9B variants to multiple sclerosis (MS) in four Northern European populations. First, 18 SNPs including 6 SNPs with previous evidence for association to immune disorders, were tested in 730 Finnish MS families, but no linkage or family-based association was observed. To ensure the power to detect variants with a modest effect size, we further analyzed 10 variants in 899 Finnish cases and 1325 controls, and in a total of 1521 cases and 1476 controls from Denmark, Norway and Sweden, but found no association. Our results thereby do not support a major function of the tested MYO9B variants in MS. European Journal of Human Genetics (2009) 17, 840-843; doi: 10.1038/ejhg.2008.251; published online 14 January 2009""Single-nucleotide polymorphisms (SNPs) in the 30 region of myosin IXB (MYO9B) gene have recently been reported to associate with different inflammatory or autoimmune diseases. We monitored for the association of MYO9B variants to multiple sclerosis (MS) in four Northern European populations. First, 18 SNPs including 6 SNPs with previous evidence for association to immune disorders, were tested in 730 Finnish MS families, but no linkage or family-based association was observed. To ensure the power to detect variants with a modest effect size, we further analyzed 10 variants in 899 Finnish cases and 1325 controls, and in a total of 1521 cases and 1476 controls from Denmark, Norway and Sweden, but found no association. Our results thereby do not support a major function of the tested MYO9B variants in MS. European Journal of Human Genetics (2009) 17, 840-843; doi: 10.1038/ejhg.2008.251; published online 14 January 2009""Single-nucleotide polymorphisms (SNPs) in the 30 region of myosin IXB (MYO9B) gene have recently been reported to associate with different inflammatory or autoimmune diseases. We monitored for the association of MYO9B variants to multiple sclerosis (MS) in four Northern European populations. First, 18 SNPs including 6 SNPs with previous evidence for association to immune disorders, were tested in 730 Finnish MS families, but no linkage or family-based association was observed. To ensure the power to detect variants with a modest effect size, we further analyzed 10 variants in 899 Finnish cases and 1325 controls, and in a total of 1521 cases and 1476 controls from Denmark, Norway and Sweden, but found no association. Our results thereby do not support a major function of the tested MYO9B variants in MS. European Journal of Human Genetics (2009) 17, 840-843; doi: 10.1038/ejhg.2008.251; published online 14 January 2009"Peer reviewe

Effects of Transmitters and Amyloid-Beta Peptide on Calcium Signals in Rat Cortical Astrocytes: Fura-2AM Measurements and Stochastic Model Simulations

BACKGROUND: To better understand the complex molecular level interactions seen in the pathogenesis of Alzheimer's disease, the results of the wet-lab and clinical studies can be complemented by mathematical models. Astrocytes are known to become reactive in Alzheimer's disease and their ionic equilibrium can be disturbed by interaction of the released and accumulated transmitters, such as serotonin, and peptides, including amyloid- peptides (A). We have here studied the effects of small amounts of A25-35 fragments on the transmitter-induced calcium signals in astrocytes by Fura-2AM fluorescence measurements and running simulations of the detected calcium signals. METHODOLOGY/PRINCIPAL FINDINGS: Intracellular calcium signals were measured in cultured rat cortical astrocytes following additions of serotonin and glutamate, or either of these transmitters together with A25-35. A25-35 increased the number of astrocytes responding to glutamate and exceedingly increased the magnitude of the serotonin-induced calcium signals. In addition to A25-35-induced effects, the contribution of intracellular calcium stores to calcium signaling was tested. When using higher stimulus frequency, the subsequent calcium peaks after the initial peak were of lower amplitude. This may indicate inadequate filling of the intracellular calcium stores between the stimuli. In order to reproduce the experimental findings, a stochastic computational model was introduced. The model takes into account the major mechanisms known to be involved in calcium signaling in astrocytes. Model simulations confirm the principal experimental findings and show the variability typical for experimental measurements. CONCLUSIONS/SIGNIFICANCE: Nanomolar A25-35 alone does not cause persistent change in the basal level of calcium in astrocytes. However, even small amounts of A25-35, together with transmitters, can have substantial synergistic effects on intracellular calcium signals. Computational modeling further helps in understanding the mechanisms associated with intracellular calcium oscillations. Modeling the mechanisms is important, as astrocytes have an essential role in regulating the neuronal microenvironment of the central nervous system

A candidate gene study of the type I interferon pathway implicates IKBKE and IL8 as risk loci for SLE

Systemic Lupus Erythematosus (SLE) is a systemic autoimmune disease in which the type I interferon pathway has a crucial role. We have previously shown that three genes in this pathway, IRF5, TYK2 and STAT4, are strongly associated with risk for SLE. Here, we investigated 78 genes involved in the type I interferon pathway to identify additional SLE susceptibility loci. First, we genotyped 896 single-nucleotide polymorphisms in these 78 genes and 14 other candidate genes in 482 Swedish SLE patients and 536 controls. Genes with P<0.01 in the initial screen were then followed up in 344 additional Swedish patients and 1299 controls. SNPs in the IKBKE, TANK, STAT1, IL8 and TRAF6 genes gave nominal signals of association with SLE in this extended Swedish cohort. To replicate these findings we extracted data from a genomewide association study on SLE performed in a US cohort. Combined analysis of the Swedish and US data, comprising a total of 2136 cases and 9694 controls, implicates IKBKE and IL8 as SLE susceptibility loci (Pmeta=0.00010 and Pmeta=0.00040, respectively). STAT1 was also associated with SLE in this cohort (Pmeta=3.3 × 10−5), but this association signal appears to be dependent of that previously reported for the neighbouring STAT4 gene. Our study suggests additional genes from the type I interferon system in SLE, and highlights genes in this pathway for further functional analysis

Intelligent tuning of pid controller for double-link flexible robotic arm manipulator by artificial bee colony algorithm

Robotics system particularly robotic arm has received tremendous demand in various fields especially manufacturing industries. Robotic arm is highly needed to enhance production, to improve output, and reduce human error. The current robotics arm not only they are expensive and required specialist for maintenance, but they are also bulky and very heavy. Thus, the option is employing lightweight, stronger, and more flexible robotics arm. However, the lightweight robotic arm can be easily influenced by unwanted vibration which may lead to problems including fatigue, instability, and performance reduction. These problems may eventually cause damage to the highly stressed structure. This research focuses on the development of intelligent controller utilizing artificial bee colony (ABC) algorithm to tune proportional integral derivative (PID) parameters for controlling two-link flexible manipulator (TLFRM). The essential objective of the designing the controller is to improve the performance of desired position and vibration suppression of TLFRM. The MATLAB environment is utilized to verify the accomplishment of the recommended control system. An assessment is conducted to illustrate the efficiency of PID-ABC controller in terms of input tracking and vibration suppression. The results show that the system with embedded new proposed controller is capable to achieve preferred angle at decrease overshoot and the settling time is exceptionally much quicker. The vibration reduction demonstrated substantial improvement as compared to manual tuning method. Overall, the proposed controller for two-link flexible manipulator that is intelligent PID-ABC was successfully control the system to the preferred position with vibration suppression in the entire system