Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae)

Cacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches' broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regulators of cellular processes and are important for the fine-tuning of plant defense responses. We developed a macroarray with 88 TF cDNA from previously obtained cacao-M. perniciosa interaction libraries. Seventy-two TFs were found differentially expressed between the susceptible (Catongo) and resistant (TSH1188) genotypes and/or during the disease time course - from 24 h to 30 days after infection. Most of the differentially expressed TFs belonged to the bZIP, MYB and WRKY families and presented opposite expression patterns in susceptible and resistant cacao-M. perniciosa interactions (i.e., up-regulated in Catongo and down-regulated in TSH1188). The results of the macroarray were confirmed for bZIP and WRKY TFs by real-time PCR. These differentially expressed TFs are good candidates for subsequent functional analysis as well as for plant engineering. Some of these TFs could also be localized on the cacao reference map related to witches' broom resistance, facilitating the breeding and selection of resistant cacao trees. (Résumé d'auteur

Suitability of wave energy converters in northwestern Spain under the near future winter wave climate

Marine renewable energies can play a key role by reducing the dependency on fossil fuels and, therefore, mitigating climate change. Among them, it is expected that wave energy will experience rapid growth in the upcoming decades. Thus, it is important to know how wave climate will change and how suitable the wave energy converters (WECs) will be to the new wave conditions. This paper aims to evaluate the capability of four different WECs—a WaveRoller type device (WRTD), Atargis, AquaBuoy and RM5—to extract wave energy on the Northwest coast of Spain (NWCS). The analysis was performed using the high-resolution wave data obtained from the Simulating Waves Nearshore (SWAN) model over the near future winters (2026–2045). The energy output (PE), the power load factor (ε), the normalized capture width (NCw) and the operational time (OT) were analyzed. According to these parameters, among the devices that work for intermediate-deep waters, Atargis would be the best option (PE=1400 ± 56 kW, ε =55.4 ± 2.2%, NCw=35.5 ± 4.1% and OT =84.5 ± 3.3%). The WRTD would also be a good option for shallow nearshore areas with PE=427 ± 248 kW, ε =12.8 ± 7.4%, NCw = 48.9 ± 9.6% and OT = 88.7 ± 18.9%. A combination of Atargis and WRTDs is proposed to make up the future wave energy farms on the NWCS.Xunta de Galicia | Ref. ED431C 2021/44Ministerio de Ciencia e Innovación | Ref. PID2020-113245RB-I00Agencia Estatal de Investigación | Ref. TED2021-129479A-100Ministerio de Ciencia e Innovación | Ref. IJC2020-043745-IAgencia Estatal de Investigación | Ref. PRE2021-097580Universidade de Vigo/CISU

Suitability of wave energy converters in northwestern Spain under the near future winter wave climate

Marine renewable energies can play a key role by reducing the dependency on fossil fuels and, therefore, mitigating climate change. Among them, it is expected that wave energy will experience rapid growth in the upcoming decades. Thus, it is important to know how wave climate will change and how suitable the wave energy converters (WECs) will be to the new wave conditions. This paper aims to evaluate the capability of four different WECs—a WaveRoller type device (WRTD), Atargis, AquaBuoy and RM5—to extract wave energy on the Northwest coast of Spain (NWCS). The analysis was performed using the high-resolution wave data obtained from the Simulating Waves Nearshore (SWAN) model over the near future winters (2026–2045). The energy output (PE), the power load factor (ε), the normalized capture width (NCw) and the operational time (OT) were analyzed. According to these parameters, among the devices that work for intermediate-deep waters, Atargis would be the best option (PE = 1400 ± 56 kW, ε = 55.4 ± 2.2%, NCw = 35.5 ± 4.1% and OT = 84.5 ± 3.3%). The WRTD would also be a good option for shallow nearshore areas with PE = 427 ± 248 kW, ε = 12.8 ± 7.4%, NCw = 48.9 ± 9.6% and OT = 88.7 ± 18.9%. A combination of Atargis and WRTDs is proposed to make up the future wave energy farms on the NWCS.publishe

Buscando uma chance para o filho vir a ser: a experiência do pai na UTI Searching a chance for the child to become: the experience of the father in the intensive care unit

O presente estudo objetiva desvelar a experiência do pai cujo filho encontra-se em Unidade de Terapia Intensiva Pediátrica, utilizando a pesquisa qualitativa, sob a perspectiva fenomenológica de Martin Heidegger, para analisar os discursos obtidos por entrevistas. Esta análise permitiu identificar três temas que configuraram aquela vivência em: Transformando seu medo em esperança, Percebendo-se como tomador de decisões e Agindo em benefício do filho. Estes temas desocultaram o fenômeno BUSCANDO UMA CHANCE PARA O FILHO VIR A SER, revelando que, para isso, o pai expõe o filho aos riscos e tratamentos que forem necessários, indicando que suas ações e a constante superação de seus sentimentos objetivam oferecer ao filhouma oportunidade para viver com plenitude.<br>This study was intended to analyse the experience of the father who have a child in an Intensive Care Unit, by applyng the qualitative research under the phenomenologycal perspective of Martin Heidegger, it was to identify three themes which figured that experience:Transforming the fear into hope,Perceiving himself as the person who decides and Acting for his child benefit. These themes unveiled the phenomenon SEARCHING A CHANCE FOR THE CHILD TO BECOME, who reveals that submits his child to risks and to essential treatments while continuously unchanging overcomes his own feelings in order to offer to his child an opportunity of full living

Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis Regeneração de plantas a partir de protoplastos de alfafa (Medicago sativa) via embriogênese somática

Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.<br>A alfafa é uma das espécies mais freqüentemente estudadas do ponto de vista da produção de embriões somáticos derivados da cultura de tecidos. Neste trabalho, cinco cultivares de alfafa foram analisados com referência à capacidade de regenerar plantas a partir de culturas de protoplastos via embriogênese somática. Regeneração de plantas a partir de protoplastos isolados de folhas da cultivar Rangelander foi obtida usando-se um protocolo definido para embriogênese somática derivada de suspensões celulares de alfafa. Em função da sua alta eficiência, recomenda-se o uso deste procedimento para transformação genética de alfafa mediada por eletroporação de protoplastos