Yeast Two-Hybrid: State of the Art

Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, the only limitation is imagination

The benefits of climate change mitigation in integrated assessment models: The role of the carbon cycle and climate component

Integrated Assessment Models (IAMs) are an important tool to compare the costs and benefits of different climate policies. Recently, attention has been given to the effect of different discounting methods and damage estimates on the results of IAMs. One aspect to which little attention has been paid is how the representation of the climate system may affect the estimated benefits of mitigation action. In that respect, we analyse several well-known IAMs, including the newest versions of FUND, DICE and PAGE. Given the role of IAMs in integrating information from different disciplines, they should ideally represent both best estimates and the ranges of anticipated climate system and carbon cycle behaviour (as e. g. synthesised in the IPCC Assessment reports). We show that in the longer term, beyond 2100, most IAM parameterisations of the carbon cycle imply lower CO 2 concentrations compared to a model that captures IPCC AR4 knowledge more closely, e. g. the carbon-cycle climate model MAGICC6. With regard to the climate component, some IAMs lead to much lower benefits of mitigation than MAGICC6. The most important reason for the underestimation of the benefits of mitigation is the failure in capturing climate dynamics correctly, which implies this could be a potential development area to focus on

Consumer Support Networks : assessment of need for consumer information and advice service

Research evidence suggests that UK consumers are facing significant problems with goods and services and are in need of information and advice to avoid or redress such situations. Consumers are not always aware of their rights nor where they can access consumer advice services. In 2000, the Department of Trade and Industry launched the Consumer Support Network (CSN) programme in Great Britain to improve consumer access to expert, accurate and timely advice. One challenge faced by these Networks and many other agencies is to assess the needs of consumers for consumer information and advice services. A needs assessment is required as a key element in the effective planning and development of services in each Network at a local level. The focus of the needs assessment at the local level is to encourage Networks to consider suitable solutions to meet the needs of people in their communities. This paper provides a review of the development of Consumer Support Networks in Great Britain and discusses the importance of needs assessment to service providers such as CSNs and other agencies. It reveals the complexity associated with conducting effective needs assessments including the various aspects of needs, consumer segmentation and characteristics of consumer information and advice. Further research is being carried out at Queen Margaret University College, UK, with a view to the development of a scientific model for the assessment of need for consumer information and advice services.div_BaM1. Department of Trade and Industry (1999) Consumer Support Networks, Getting Connected . Department of Trade and Industry, London. 2. Department of Trade and Industry (1999) Modern Markets: Confident Consumers . The Stationery Office, London. 3. London Economics (1997) Consumer Detriment Under Conditions of Imperfect Information , Office of Fair Trading, London. 4. 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