The effects of medium-oil dried distillers grains with solubles on growth performance and carcass traits in finishing pigs

An experiment was conducted to determine the effects of increasing medium-oil dried distillers grains with solubles (DDGS; 7.4% fat, 28.1% CP, 10.8% ADF, and 25.6% NDF) on growth performance and carcass traits in finishing pigs. A total of 288 pigs (PIC 327 × 1050; initially 151.8 lb) were allotted to 1 of 4 dietary treatments. Treatments consisted of a corn-soybean meal control diet or the control diet with 15, 30, or 45% medium-oil DDGS, with 8 pigs per pen and 8 replications per treatment. Increasing medium-oil DDGS decreased (linear, P < 0.01) ADG and worsened (linear, P < 0.02) F/G. In addition, final BW, HCW, carcass yield, and loin-eye depth decreased (linear, P < 0.03), and jowl iodine value (IV) increased (linear, P < 0.001) with increasing medium-oil DDGS. When pigs are fed traditional DDGS containing >10.5% fat, each 10% DDGS added to the diet increases jowl IV approximately 2 mg/g; however, feeding increasing medium-oil DDGS increased jowl IV only about 1.4 units per each 10% DDGS. In conclusion, swine producers must be aware of the negative ramifications on growth performance of using medium-oil DDGS in swine diets

The interactive effects of high-fiber diets and Ractopamine HCl on finishing pig growth performance, carcass characteristics, carcass fat quality, and intestinal weights

In previous research, feeding pigs high amounts of dried distillers grains with solubles (DDGS) and wheat middlings (midds) has been shown to reduce carcass yield and negatively affect iodine value (IV). The influence of Ractopamine HCl (RAC; Paylean, Elanco Animal Health, Greenfield, IN) on this response is not known; therefore, a total of 575 finishing pigs (PIC 327 × 1050, initially 123 lb) were used in two consecutive 73-d trials to determine the effects of DDGS and midds (high fiber) withdrawal 24 d before harvest in diets with or without RAC on finishing pig growth performance, carcass characteristics, and fat quality. From d 0 to 49, pigs were allotted to 1 of 2 dietary treatments in a completely randomized design based on initial pen weight. The dietary treatments included a corn-soybean meal–based control diet or diets with 30% DDGS and 19% wheat midds. Twelve pens of pigs were fed the corn-soybean meal control diet, and 24 pens were fed the high-fiber diet. During this 49 d period, pigs fed the corn-soybean meal diets had improved (P < 0.0001) ADG and F/G compared with those fed the high-fiber diets. On d 49, pens of pigs were re-allotted to 1 of 6 dietary treatments; pigs remained on the corn-soybean meal diets, switched from the high-fiber diet to corn-soybean meal (withdrawal diet), or were maintained on the high-fiber diet. These 3 regimens were fed with or without 9 g/ton RAC

Assessing cycling skills in Switzerland

This paper sets out to identify skills required by cyclists to navigate safely through an urban environment in Switzerland. We set out to identify situations that might result in accidents and require specific competences. Three study studies were conducted First, workshops with experts were conducted to identify required cyclists' skills. Second, accident statistics were analysed to determine in which type of situations accidents occur. Finally, a survey was conducted among Swiss cyclists to assess which skills were present and which skills were lacking. The remainder of this extended abstract describes the three studies in some detail and continues with an outlook for the final paper. [from Introduction

mRNA transfection of mouse and human neural stem cell cultures

The use of synthetic mRNA as an alternative gene delivery vector to traditional DNA-based constructs provides an effective method for inducing transient gene expression in cell cultures without genetic modification. Delivery of mRNA has been proposed as a safer alternative to viral vectors in the induction of pluripotent cells for regenerative therapies. Although mRNA transfection of fibroblasts, dendritic and embryonic stem cells has been described, mRNA delivery to neurosphere cultures has not been previously reported. Here we sought to establish an efficient method for delivering mRNA to primary neurosphere cultures. Neurospheres derived from the subventricular zone of adult mice or from human embryonic stem cells were transfected with EGFP mRNA by lipofection and electroporation. Transfection efficiency and expression levels were monitored by flow cytometry. Cell survival following transfection was examined using live cell counting and the MTT assay. Both lipofection and electroporation provided high efficiency transfection of neurospheres. In comparison with lipofection, electroporation resulted in increased transfection efficiencies, but lower expression per cell and shorter durations of expression. Additional rounds of lipofection renewed EGFP expression in neurospheres, suggesting this method may be suitable for reprogramming applications. In summary, we have developed a protocol for achieving high efficiency transfection rates in mouse and human neurosphere cell culture that can be applied for future studies of gene function studies in neural stem cells, such as defining efficient differentiation protocols for glial and neuronal linages

Crossover and scaling in a two-dimensional field-tuned superconductor

Using an analysis similar to that of Imry and Wortis, it is shown that the apparent first order superconductor to metal transition, which has been claimed to exist at low values of the magnetic field in a two-dimensional field-tuned system at zero temperature,can be consistentlyinterpreted as a sharp crossover from a strong superconductor to an inhomogeneous state, which is a weak superconductor. The true zero-temperature superconductor to insulator transition within the inhomogenous state is conjectured to be that of randomly diluted XY model. An explaination of the observed finite temperature approximate scaling of resistivity close to the critical point is speculated within this model.Comment: 5 pages, 2 figures, corrected and modified according to referee Report

The yeast Pan2 protein is required for poly(A)-binding protein-stimulated poly(A)-nuclease activity

The removal of the mRNA poly(A) tail in the yeast Saccharomyces cerevisiae is stimulated by the poly(A)binding protein (Pab1p). A large scale purification of the Pab1p-stimulated poly(A) ribonuclease (PAN) identifies a 76-kDa and two 135-Da polypeptides as candidate enzyme subunits. Antibodies against the Pan1p protein, which is the minor 135-kDa protein in the preparation, can immunodeplete Pan1p but not PAN activity. The protein sequence of the major 135-kDa protein, Pan2p, reveals a novel protein that was also found in the previously reported PAN purification (Sachs, A. B., and Deardorff, J. A. (1992) Cell 70, 961-973). Deletion of the non-essential PAN2 gene results in an increase of the average length of mRNA poly(A) tails in vivo, and a loss of Pab1p-stimulated PAN activity in crude extracts. These data confirm that Pan2p and not Pan1p is required for PAN activity, and they suggest that ribonucleases other than the Pab1p-stimulated PAN are capable of shortening poly(A) tails in vivo

Spacetime Energy Decreases under World-sheet RG Flow

We study renormalization group flows in unitary two dimensional sigma models with asymptotically flat target spaces. Applying an infrared cutoff to the target space, we use the Zamolodchikov c-theorem to demonstrate that the target space ADM energy of the UV fixed point is greater than that of the IR fixed point: spacetime energy decreases under world-sheet RG flow. This result mirrors the well understood decrease of spacetime Bondi energy in the time evolution process of tachyon condensation.Comment: 25 pages, 4 figures, harvma

The role of location in residential location choice models: a review of literature

JTLU vol. 7, no. 2, pp. 3-21 (2014)Geospatial data available to researchers has increased tremendously over the last several decades, opening up opportunities to define residential location in multiple ways. This has led to a myriad of variables to define "location'' in residential location choice models. In this paper, we propose a common classification for location variables and categorize findings from a wide range of studies. We find similar preferences but different measurement methods and market segments for locations across different study regions. Recent studies consider the residential unit as choice alternative, making it possible to include a detailed description of the built environment. However, these studies are still limited in number and the inclusion of socioeconomic environment is more common. Transport land-use models can benefit from the inclusion of points of interest, such as schools, network distances, and the distance to previous locations. For the results of location choice models to be transferable to different disciplines, and avoid multi-collinearity, it is necessary to present different model specifications, including variables of interest in different disciplines