Identification, sequencing and expression of the glycoprotein-L gene of murine cytomegalovirus

DNA sequence analysis of the genome of the Smith strain of murine cytomegalovirus (MCMV) revealed an open reading frame (ORF) with amino acid sequence identity to glycoprotein L (gL) of other herpesviruses. The ORF is 822 bp in size and has the capacity to encode a protein of 274 amino acids. It has significant identity with the gL genes of human CMV and human herpesvirus 6. The coding sequence of the gL gene of MCMV strain K181 was also determined, and expressed in Escherichia coli as a fusion protein with glutathione S-transferase using the pGEX expression system. Two antibody-binding regions were identified on the basis of the reactivity of a series of truncated gL constructs with anti-MCMV antibodies. One was mapped to residues 1 to 38 and the other between residues 230 and 274. Polyclonal antibodies specific to gL were raised against the full-length gL fusion protein. The antisera were shown to react with a 46K protein present in purified virions by Western blotting. Treatment of purified virions with endoglycosidase-H or -F resulted in reductions in M(r) of the 46K species to 42K and 31K, respectively. The antisera did not exhibit any neutralizing activity in a plaque reduction assay

Molecular and biological characterization of new strains of murine cytomegalovirus isolated from wild mice

Studies of the prevalence of antibody to murine cytomegalovirus (MCMV) in free-living wild mice (Mus domesticus) trapped in diverse regions of Australia and on a sub-Antarctic island indicated that 90% of 468 mice had serum antibody to MCMV. Twenty-six field isolates of MCMV were plaque-purified from salivary gland extracts of representative seropositive mice. These isolates varied considerably in their ability to replicate in the salivary glands of weanling BALB/c mice with 9 of 15 failing to reach significant titres in this organ and the titres of the remaining 6 strains varying by at least 100-fold. The high frequency of restriction fragment length polymorphisms observed suggests widespread genetic heterogeneity exists among the strains. This observation was mirrored at the polypeptide level by Western blot analyses with polyclonal antisera to MCMV. The isolation in this study of four genetically distinct strains of MCMV from a single wild mouse and several strains from other individual mice demonstrates that multiple infections with MCMV may be commonplace in wild mice

OzDES multi-object fibre spectroscopy for the Dark Energy Survey: results and second data release

We present a description of the Australian Dark Energy Survey (OzDES) and summarize the results from its 6 years of operations. Using the 2dF fibre positioner and AAOmega spectrograph on the 3.9-m Anglo-Australian Telescope, OzDES has monitored 771 active galactic nuclei, classified hundreds of supernovae, and obtained redshifts for thousands of galaxies that hosted a transient within the 10 deep fields of the Dark Energy Survey. We also present the second OzDES data release, containing the redshifts of almost 30 000 sources, some as faint as rAB = 24 mag, and 375 000 individual spectra. These data, in combination with the time-series photometry from the Dark Energy Survey, will be used to measure the expansion history of the Universe out to z ∼ 1.2 and the masses of hundreds of black holes out to z ∼ 4. OzDES is a template for future surveys that combine simultaneous monitoring of targets with wide-field imaging cameras and wide-field multi-object spectrographs

Multi-messenger Observations of a Binary Neutron Star Merger

International audienceOn 2017 August 17 a binary neutron star coalescence candidate (later designated GW170817) with merger time 12:41:04 UTC was observed through gravitational waves by the Advanced LIGO and Advanced Virgo detectors. The Fermi Gamma-ray Burst Monitor independently detected a gamma-ray burst (GRB 170817A) with a time delay of $\sim 1.7\,{\rm{s}}$ with respect to the merger time. From the gravitational-wave signal, the source was initially localized to a sky region of 31 deg(2) at a luminosity distance of ${40}_{-8}^{+8}$ Mpc and with component masses consistent with neutron stars. The component masses were later measured to be in the range 0.86 to 2.26 $\,{M}_{\odot }$. An extensive observing campaign was launched across the electromagnetic spectrum leading to the discovery of a bright optical transient (SSS17a, now with the IAU identification of AT 2017gfo) in NGC 4993 (at $\sim 40\,{\rm{Mpc}}$) less than 11 hours after the merger by the One-Meter, Two Hemisphere (1M2H) team using the 1 m Swope Telescope. The optical transient was independently detected by multiple teams within an hour. Subsequent observations targeted the object and its environment. Early ultraviolet observations revealed a blue transient that faded within 48 hours. Optical and infrared observations showed a redward evolution over ∼10 days. Following early non-detections, X-ray and radio emission were discovered at the transient’s position $\sim 9$ and $\sim 16$ days, respectively, after the merger. Both the X-ray and radio emission likely arise from a physical process that is distinct from the one that generates the UV/optical/near-infrared emission. No ultra-high-energy gamma-rays and no neutrino candidates consistent with the source were found in follow-up searches. These observations support the hypothesis that GW170817 was produced by the merger of two neutron stars in NGC 4993 followed by a short gamma-ray burst (GRB 170817A) and a kilonova/macronova powered by the radioactive decay of r-process nuclei synthesized in the ejecta