Association of interleukin 1 gene cluster and interleukin 1 receptor gene polymorphisms with ischemic heart failure

BACKGROUND: Proinfl ammatory cytokines have been known to play a considerable part in the pathomechanisms of chronic heart failure (CHF). Given the importance of proinfl ammatory cytokines in the context of the failing heart, we assessed whether the polymorphisms of interleukin (IL)-1 gene cluster, including IL-1a, IL-1β, and IL-1 receptor antagonist (IL-1RA) and IL-1R gene are predictors of CHF due to ischemic heart disease. METHODS: Forty- three patients with ischemic heart failure were recruited in this study as patients group and compared with 140 healthy unrelated control subjects. Using polymerase chain reaction with sequence-specifi c primers method, the allele and genotype frequency of 5 single nucleotide polymorphisms (SNPs) within the IL- 1a (-889), IL-1β (-511, +3962), IL-1R (psti 1970), and IL-1RA (mspa1 11100) genes were determined.RESULTS: The frequency of the IL-1β -511/C allele was signifi cantly higher in the patient group compared to that in the control group (p = 0.031). The IL-1β (-511) C/C genotype was signifi cantly overrepresented in patients compared to controls (p = 0.022). CONCLUSIONS: Particular allele and genotype in IL-1β gene were overrepresented in patients with ischemic heart failure, possibly affecting the individual susceptibility to this disease (Tab. 1, Ref. 27). Text in PDF www.elis.sk

Prevalence of hepatitis D virus infection in HBsAg positive subjects in Iran

Abstract This study was carried out to determine the seroprevalence of hepatitis D virus among HBsAg positive individuals in the northeast part of Iran. One hundred thirty nine HBsAg positive subjects detected from a population based single stage cluster sampling in Golestan province of Iran were enrolled. All cases were evaluated for the presence of anti-HDV antibodies using commercially available ELISA kits. Logistic regression was used to determine the relationship between independent variables and HDV seropositivity. Of 139 cases, 68(48.9%) were males and 71(51.1%) were females. The mean age was 41.89±11.30 years (25-64 years). Anti-HDV antibody was positive in 8 (5.8%) subjects with female predominance (9.9% versus 1.5%, p = 0.06; odds ratio = 7.32, 95%CI: 0.87-61.23). No significant relationship was seen between anti-HDV seropositivity and demographic factors such as age, place of residence and marital status. These findings showed that HDV infection was endemic in Golestan province (northeast) of Iran. Seroprevalence of Anti-HDV in the present study was higher than some previous studies from other parts of Iran. Our results suggest that the prevalence of HBV/HDV co-infection in Iran has increased during the last decade. Therefore, practitioners and all health care managers should be made aware of the risk of dual infection with HBV and HDV. © 2007 Asian Network for Scientific Information

Interleukin-2 and Interferon-Gamma single nucleotide polymorphisms in Iranian patients with chronic heart failure

Background: Inflammatory cytokines have been known to be associated with Chronic Heart Failure (CHF). Given the importance of cytokines in the context of the failing heart, the prevalence of Interleukin-2 (IL-2) and Interferon-gamma (IFN-γ) polymorphisms was studied in patients with CHF due to ischemic heart disease in a case-control study. Methods: Fifty-six Iranian patients with CHF were enrolled in this study as the case group and compared with 139 healthy subjects, using polymerase chain reaction with sequence-specific primers method, so as to determine the frequency of alleles, genotypes and haplotypes of IFN-γ (+874 A/T) and IL-2 (-330 G/T, +166 G/T) SNPs. Results: The GG genotype at IL-2-330 in patients with CHF was significantly overrepresented in comparison with the control group (p=0.013). Such a positive genotypic association was also observed for IL-2 +166/TT (p=0.022). Meanwhile, the GT genotype frequency at IL-2-330/GT in the patient group was significantly lower than the one in healthy controls (p=0.049). No significant association was detected between the IFN-γ gene polymorphisms and individuals� susceptibility to CHF. Conclusion: Certain genotypes in IL-2 gene were overrepresented in patients with CHF, which could render individuals more vulnerable to this disease. © 2018, Avicenna Journal of Medical Biotechnology. All rights reserved

FE parametric study of RWS/WUF-B moment connections with elliptically-based beam web openings under monotonic and cyclic loading

This paper provides numerical results investigating the behaviour of steel web-perforated beams with different shaped single openings located close to beam-to-column connections under monotonic and cyclic loading. In particular, the beams considered feature circular and patented elliptically-based perforations. Non-standard elliptically-based perforations have been proposed previously and are optimally designed to maximise resistance against Vierendeel moments and web-post buckling under static loads at the ultimate limit state. Comprehensive parametric nonlinear finite element analyses using the commercial FE package ANSYS were conducted. Initially, a FE model of the beam-to-column WUF-B moment connection was developed and calibrated against pertinent experimental results found in the literature. Next, parametric analyses were undertaken to assess the RWS/WUF-B connection regarding strength (moment), deformation (rotation) and column web shear panel zone deformation for different shapes of beam web perforations, hole sizes, and their locations. The study concludes that larger web openings are capable of moving the plastic hinge away from the column face and the CJP weld. Also, interstory drifts can be controlled with the wise use of the beam web opening size, shape, and distance from the face of the column, as suggested in the paper. Following, a step-by-step design process for RWS/WUF-B connection is presented