1,915 research outputs found
In Vivo Bacterial Morphogenetic Protein Interactions
This chapter will discuss none-invasive techniques that are widely used to study protein-protein interactions. As an example, their application in exploring interactions between proteins involved in bacterial cell division will be evaluated. First, bacterial morphology and cell division of the rod-shaped bacterium Escherichia coli will be introduced. Next, three bacterial two-hybrid methods and three Förster resonance energy transfer detection methods that are frequently applied to detect interactions between proteins will be described and discussed in detail. The chapter concludes with a discussion about the application and results of the techniques when studying proteins involved in cell division
Chapter In vivo bacterial morphogenetic protein interactions
Aquaculture & fish-farming: practice & technique
The relationships between internal and external threat and right-wing attitudes: A three-wave longitudinal study
The interplay between threat and right-wing attitudes has received much research attention, but its longitudinal relationship has hardly been investigated. In this study, we investigated the longitudinal relationships between internal and external threat and right-wing attitudes using a cross-lagged design at three different time points in a large nationally representative sample (N = 800). We found evidence for bidirectional relationships. Higher levels of external threat were related to higher levels of Right-Wing Authoritarianism and to both the egalitarianism and dominance dimensions of Social Dominance Orientation at a later point in time. Conversely, higher levels of RWA were also related to increased perception of external threat later in time. Internal threat did not yield significant direct or indirect longitudinal relationships with right-wing attitudes. Theoretical and practical implications of these longitudinal effects are discussed
Four-qubit device with mixed couplings
We present the first experimental results on a device with more than two
superconducting qubits. The circuit consists of four three-junction flux
qubits, with simultaneous ferro- and antiferromagnetic coupling implemented
using shared Josephson junctions. Its response, which is dominated by the
ground state, is characterized using low-frequency impedance measurement with a
superconducting tank circuit coupled to the qubits. The results are found to be
in excellent agreement with the quantum-mechanical predictions.Comment: REVTeX 4, 5pp., 7 EPS figure files. N.B.: "Alec" is my first, and
"Maassen van den Brink" my family name. v2: final published version, with
changed title, different sample micrograph, and several clarification
The Rascal meta-programming language - a lab for software analysis, transformation, generation & visualization
National audienceThis paper summarizes the goals and features of a do- main specific programming language called Rascal. On the one hand it is designed to facilitate software research -- research about software in general. On the other hand Rascal is applied to specific software portfolios as well, as a means to improve them and as a means to learn to understand them. Specifically, Rascal is used create tools that analyze, transform, generate or visualize source code of software products. Such tools are motivated by the need to im- prove quality of existing software or the need to lower its cost-of-ownership. More generally such tools are cre- ated to build laboratory experiments that observe and measure quality, or try and improve software quality, etc. In this paper we provide an overview of Rascal as a "domain specific language for meta programming". We first explain its goals and then its features. We end by highlighting some example applications in the area of software analysis and transformation
Extended preservation and effect of nitric oxide production in liver transplantation
Liver transplantation (Ltx) has become a routine procedure for patients with end-stage liver disease. Despite ongoing progress on short- and long-term graft survival, primary dysfunction (PDF) remains a major problem. PDF is significantly associated with the duration of cold ischemia- and, possibly, with reperfusion-related injury. Nitric oxide (NO) has many physiological functions and plays an important role in modulating tissue injury. However, the mechanism of NO action in ischemia/reperfusion injury after Ltx is thus far unknown. In this study we investigated the role of inducable NO synthase (iNOS) in the liver after preservation with UW solution using the orthotopic Ltx model in the rat. Male Brown Norway rats were used for the Ltx procedure. After donor hepatectomy, livers were stored on ice-cold UW solution for 24 or 40 h and subsequently transplanted. A control group consisted of rats with Ltx after less than 1 h storage. Posttransplant blood samples were taken at 48 h to determine standard parameters for liver injury (aspartate transaminase, lactate dehydrogenase). Liver biopsies were obtained for detection of expression of iNOS (western blot) 24 and 48 h posttransplant. We observed that a preservation time of 24 h in UW solution presents no problem for graft survival after Ltx in rats with some brain function and in healthy animals. After 40 h preservation, liver damage is obvious and graft survival reduced, indicating the limits of cold storage may be within reach. With longer preservation times, more NOs was detected in liver tissue. This finding suggests that NO has a role in ischemia/reperfusion-related injury. Current intervention with NOS inhibitors will reveal whether NO has a negative or a positive effect on graft survival after Ltx.</p
Coupling Superconducting Qubits via a Cavity Bus
Superconducting circuits are promising candidates for constructing quantum
bits (qubits) in a quantum computer; single-qubit operations are now routine,
and several examples of two qubit interactions and gates having been
demonstrated. These experiments show that two nearby qubits can be readily
coupled with local interactions. Performing gates between an arbitrary pair of
distant qubits is highly desirable for any quantum computer architecture, but
has not yet been demonstrated. An efficient way to achieve this goal is to
couple the qubits to a quantum bus, which distributes quantum information among
the qubits. Here we show the implementation of such a quantum bus, using
microwave photons confined in a transmission line cavity, to couple two
superconducting qubits on opposite sides of a chip. The interaction is mediated
by the exchange of virtual rather than real photons, avoiding cavity induced
loss. Using fast control of the qubits to switch the coupling effectively on
and off, we demonstrate coherent transfer of quantum states between the qubits.
The cavity is also used to perform multiplexed control and measurement of the
qubit states. This approach can be expanded to more than two qubits, and is an
attractive architecture for quantum information processing on a chip.Comment: 6 pages, 4 figures, to be published in Natur
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