In situ High Pressure and Temperature 13C NMR for the Study of Carbonation Reactions of CO2

The aqueous reactions of carbon dioxide with various Mg-containing minerals [MgO, Mg(OH)2, and Mg2SiO4] at several different pressures: 1-200 bar) and temperatures: 25-150C) have been studied using a novel, elevated pressure and temperature 13C NMR probe. Critical observations about reaction rates, chemical exchange, and pH measurements throughout these reactions and the implications of the in situ measurements made during these reactions are discussed. A new method is used to elucidate pH under high pressure and temperature conditions which utilizes a calculation scheme wherein experimental data and a computational model are combined. Additionally, a 1D pH imaging method is employed to observe pH gradient effects across mineral samples during their reaction with CO2. Finally, other experimental details are discussed including ex situ analysis on carbonate products using pXRD, Raman, and MAS NMR. Detailed discussion outlines how to use 13C NMR to study CO2 mineralization reactions

GENERAL DESCRIPTION PINNING SOT23 P-channel, enhancement mode, PIN DESCRIPTION

logic level, field-effect power transistor. This device has low 1 gate threshold voltage and extremely fast switching making it ideal for 2 source battery powered applications and high speed digital interfacing. 3 drain 3 Top view The BSH201 is supplied in the SOT23 subminiature surface mounting package

When Diversity Measures Are Nonequivalent: Advice for Practitioners

When addressing diversity, equity, and inclusion, researchers and organizations often focus on group differences in outcomes of interest. However, groups do not always interpret surveys in the same way, causing measurement nonequivalence. Measurement nonequivalence makes it difficult, if not impossible, to compare group differences presenting a problem for how conclusions are drawn. To better understand group differences in survey responding, the current study assessed measurement invariance across five diversity-related measures using the methods outlined by Nye and colleagues (Nye et al., 2019; Somaraju et al., 2022). Data were collected across three organizations (N = 732) from different industries (i.e., healthcare, construction, information technology). Results indicate that for all five measures, there was significant measurement nonequivalence across organizations such that all but the referent item were found to be nonequivalent. We also examined measurement invariance across race and gender where all measures in all organizations were nonequivalent. Interestingly, these effects were not similar across organizations. The construction company had strong gender effects across measures (dMAC = -.64 to -.13), but weak racial effects (dMAC = -.08 to .34). In contrast, the healthcare company had relatively stronger racial effects (dMAC = -.62 to -.35) than gender (dMAC = -.43 to -.01). The information technology company had low effects for both race (dMAC = -.29 to .04) and gender (dMAC = -.20 to .09). Given these results, there are several implications for both research and practice. Researchers should not assume that samples collected across multiple organizations are equivalent and the use of hierarchically nest models may be necessary to account for group differences. Further, greater attention is needed in measurement development to ensure their validity across groups. For practitioners, we recommend utilizing open-ended survey items to better capture group differences due to the prevalence of high measurement nonequivalence in closed-items.https://digitalcommons.odu.edu/gradposters2023_sciences/1001/thumbnail.jp

Identification of a transporter complex responsible for the cytosolic entry of nitrogen-containing bisphosphonates

Nitrogen-containing-bisphosphonates (N-BPs) are widely prescribed to treat osteoporosis and other bone-related diseases. Although previous studies established that N-BPs function by inhibiting the mevalonate pathway in osteoclasts, the mechanism by which N-BPs enter the cytosol from the extracellular space to reach their molecular target is not understood. Here we implemented a CRISPRi-mediated genome-wide screen and identified SLC37A3 (solute carrier family 37 member A3) as a gene required for the action of N-BPs in mammalian cells. We observed that SLC37A3 forms a complex with ATRAID (all-trans retinoic acid-induced differentiation factor), a previously identified genetic target of N-BPs. SLC37A3 and ATRAID localize to lysosomes and are required for releasing N-BP molecules that have trafficked to lysosomes through fluid-phase endocytosis into the cytosol. Our results elucidate the route by which N-BPs are delivered to their molecular target, addressing a key aspect of the mechanism of action of N-BPs that may have significant clinical relevance

Dynamics of glomerular ultrafiltration: VI. Studies in the primate

Dynamics of glomerular ultrafiltration: VI. Studies in the primate. Pressures and flows were measured in accessible surface glomeruli of the squirrel monkey under conditions of normal hydropenia. Mean glomerular capillary hydrostatic pressure and the mean glomerular transcapillary hydrostatic pressure difference (ΔP) averaged approximately 45 mm Hg and 35 mm Hg, respectively. These findings are in close accord with recent direct estimates in the rat. The net driving force for ultrafiltration was found to decline from a maximum value of about 12 mm Hg at the afferent end of the glomerular capillary network essentially to zero by the efferent end, indicating that, in the monkey as in the rat, filtration pressure equilibrium is achieved under normal hydropenic conditions. The monkey differs from the rat in one important respect, however, in that, as has long been recognized, the monkey tends to have higher systemic total plasma protein concentrations (CA) than the rat. This is of interest since monkey, like man, is found to have lower filtration fractions than the rat. Since ΔP is found to be essentially similar in monkey and rat, and since, at filtration pressure equilibrium, filtration fraction is determined by ΔP and CA, these observed differences in filtration fraction between rodent and primate must therefore be due to these differences in CA

Nonmetropolitan Nebraskans’ Perceptions of Local Schools: 2016 Nebraska Rural Poll Results

Conclusion Most rural Nebraskans have elementary, middle and high schools in their community or nearest community. Most of the schools available are public but many rural Nebraskans report having both public and private schools of each grade level. Persons living in or near larger communities are more likely than persons living in or near smaller communities to have each level of school in their community. And, persons living in or near the larger communities are more likely than persons living in or near smaller communities to have both public and private schools available. When asked about priorities for their local school, rural Nebraskans give highest priority to providing a safe environment for students. Other top priorities for local schools include: high graduation rate, high quality teachers, teaching problem solving or critical thinking skills to students and preparing students for college. The items that respondents were least likely to rate as high priority include teaching foreign languages, providing community social events/local entertainment, and teaching English as a second language. At least four in ten rural Nebraskans are very satisfied with the following characteristics at their local school: safe environment for students, graduation rate, quality school buildings, and opportunities for physical activities/sports. Less than two in ten respondents are very satisfied with standardized test scores and teaching English as a second language. However, many rural Nebraskans are unsure how to assess the details of their local schools since at least two in ten answered don’t know when asked to rate the characteristics. In fact, four in ten answered don’t know in regards to providing courses for advanced placement and standardized test scores. And, over one-half answered don’t know when asked to rate their satisfaction with teaching English as a second language. Satisfaction levels with many characteristics differ by community size. Persons living in or near smaller communities are more likely than persons living in or near larger communities to be very satisfied with many of the characteristics at their local school. However, satisfaction levels with four items increase as community size increases: providing courses for college credit, providing advanced placement courses, teaching foreign languages, and teaching English as a second language. Regional differences also exist. For many of the items listed, residents of the Panhandle were the regional group least likely to report being very satisfied. However, even though the Panhandle residents are less likely to report being very satisfied with many of the items listed, it does not mean they are necessarily more dissatisfied with those items than the other groups. For many of the items, the Panhandle residents were more likely to answer “don’t know.” The differences between the characteristics that people expect from their local school and those with which they are very satisfied are rather large. For almost all of the characteristics listed, the proportions that feel each is a high priority are larger than the proportions very satisfied with each. For many of the characteristics, the differences between the proportions viewing the characteristics as high priorities and the proportions very satisfied with each are larger for persons living in or near larger communities as compared to persons living in or near smaller communities. For a few items, the gaps are somewhat larger for persons living in or near smaller communities: providing courses for college credit, preparing students for technical/trade school, and providing advanced placement courses

Braveheart, a Long Noncoding RNA Required for Cardiovascular Lineage Commitment

Long noncoding RNAs (lncRNAs) are often expressed in a development-specific manner, yet little is known about their roles in lineage commitment. Here, we identified Braveheart (Bvht), a heart-associated lncRNA in mouse. Using multiple embryonic stem cell (ESC) differentiation strategies, we show that Bvht is required for progression of nascent mesoderm toward a cardiac fate. We find that Bvht is necessary for activation of a core cardiovascular gene network and functions upstream of mesoderm posterior 1 (MesP1), a master regulator of a common multipotent cardiovascular progenitor. We also show that Bvht interacts with SUZ12, a component of polycomb-repressive complex 2 (PRC2), during cardiomyocyte differentiation, suggesting that Bvht mediates epigenetic regulation of cardiac commitment. Finally, we demonstrate a role for Bvht in maintaining cardiac fate in neonatal cardiomyocytes. Together, our work provides evidence for a long noncoding RNA with critical roles in the establishment of the cardiovascular lineage during mammalian development.Damon Runyon Cancer Research Foundation (DRG 2032-09)Damon Runyon Cancer Research Foundation (DFS 04-12)European Molecular Biology Organization (Long-term Fellowship)National Heart, Lung, and Blood Institute. Bench to Bassinet Program (U01HL098179)National Heart, Lung, and Blood Institute. Bench to Bassinet Program (U01HL098188)Smith Family FoundationPew Charitable Trusts. Program in the Biomedical Science

Casimir effect due to a single boundary as a manifestation of the Weyl problem

The Casimir self-energy of a boundary is ultraviolet-divergent. In many cases the divergences can be eliminated by methods such as zeta-function regularization or through physical arguments (ultraviolet transparency of the boundary would provide a cutoff). Using the example of a massless scalar field theory with a single Dirichlet boundary we explore the relationship between such approaches, with the goal of better understanding the origin of the divergences. We are guided by the insight due to Dowker and Kennedy (1978) and Deutsch and Candelas (1979), that the divergences represent measurable effects that can be interpreted with the aid of the theory of the asymptotic distribution of eigenvalues of the Laplacian discussed by Weyl. In many cases the Casimir self-energy is the sum of cutoff-dependent (Weyl) terms having geometrical origin, and an "intrinsic" term that is independent of the cutoff. The Weyl terms make a measurable contribution to the physical situation even when regularization methods succeed in isolating the intrinsic part. Regularization methods fail when the Weyl terms and intrinsic parts of the Casimir effect cannot be clearly separated. Specifically, we demonstrate that the Casimir self-energy of a smooth boundary in two dimensions is a sum of two Weyl terms (exhibiting quadratic and logarithmic cutoff dependence), a geometrical term that is independent of cutoff, and a non-geometrical intrinsic term. As by-products we resolve the puzzle of the divergent Casimir force on a ring and correct the sign of the coefficient of linear tension of the Dirichlet line predicted in earlier treatments.Comment: 13 pages, 1 figure, minor changes to the text, extra references added, version to be published in J. Phys.