Kisspeptin receptor on the sperm surface reflects epididymal maturation in the dog

Alongside the well-known central modulatory role, the Kisspeptin system, comprising Kiss1, its cleavage products (Kisspeptins), and Kisspeptin receptor (Kiss1R), was found to regulate gonadal functions in vertebrates; however, its functional role in the male gamete and its localization during maturation have been poorly understood. The present study analyzed Kisspeptin system in dog testis and spermatozoa recovered from different segments of the epididymis, with focus on Kiss1R on sperm surface alongside the maturation during epididymal transit, demonstrated by modification in sperm kinetic, morphology, and protamination. The proteins Kiss1 and Kiss1R were detected in dog testis. The receptor Kiss1R only was detected in total protein extracts from epididymis spermatozoa, whereas dot blot revealed Kiss1 immunoreactivity in the epidydimal fluid. An increase of the Kiss1R protein on sperm surface along the length of the epididymis, with spermatozoa in the tail showing plasma membrane integrity and Kiss1R protein (p < 0.05 vs. epididymis head and body) was observed by flow cytometry and further confirmed by epifluorescence microscopy and Western blot carried on sperm membrane preparations. In parallel, during the transit in the epididymis spermatozoa significantly modified their ability to move and the pattern of motility; a progressive increase in protaminization also occurred. In conclusion, Kisspeptin system was detected in dog testis and spermatozoa. Kiss1R trafficking toward plasma membrane along the length of the epididymis and Kiss1 in epididymal fluid suggested a new functional role of the Kisspeptin system in sperm maturation and storage

Ferromagnetism in the Hubbard model with Topological/Non-Topological Flat Bands

We introduce and study two classes of Hubbard models with magnetic flux or with spin-orbit coupling, which have a flat lowest band separated from other bands by a nonzero gap. We study the Chern number of the flat bands, and find that it is zero for the first class but can be nontrivial in the second. We also prove that the introduction of on-site Coulomb repulsion leads to ferromagnetism in both the classes.Comment: 6 pages, 5 figure

Nature and Climate Change Effects on Economic Growth: An LSTM Experiment on Renewable Energy Resources

Global energy demand increases overtime, especially in emerging market economies, producing potential negative environmental impacts, particularly on the long term, on nature and climate changes. Promoting renewables is a robust policy action in world energy-based economies. This study examines if an increase in renewables production has a positive effect on the Brazilian economy, partially offsetting the SARS-CoV2 outbreak recession. Using data on Brazilian economy, we test the contribution of renewables on the economy via a ML architecture (through a LSTM model). Empirical findings show that an ever-greater use of renewables may sustain the economic growth recovery, generating a better performing GDP acceleration vs. other energy variables

Comparison of fatty acid profile in lamb meat and baby food based on lamb meat

The aim of this study was to compare the fatty acid (FA) profile of fresh lamb meat with those of baby foods based on lamb meat. For this purpose, samples of commercial homogenized (HO) and lyophilized (LIO) baby food based on lamb meat and fresh lamb meat (FM) were analyzed for their FA composition. All fatty acids, except for oleic acid and conjugated linoleic acid (CLA), differed among the three baby products tested. The sum of omega-6 FA and the ratio omega 6/omega 3 FA were lower in FM and LIO meat compared to HO samples. The content of total PUFA n-3 was the highest in FM, because of its highest content of C18:3 n3, EPA, DPA and DHA compared to LIO and HO baby food. The content of arachidonic acid was more than 6-fold higher in FM compared to LIO and HO. This study evidenced the possibility of enhancing the FA profile of commercial baby food based on lamb meat by using meat with healthier FA profile

The Ontology of Intentional Agency in Light of Neurobiological Determinism: Philosophy Meets Folk Psychology

The moot point of the Western philosophical rhetoric about free will consists in examining whether the claim of authorship to intentional, deliberative actions fits into or is undermined by a one-way causal framework of determinism. Philosophers who think that reconciliation between the two is possible are known as metaphysical compatibilists. However, there are philosophers populating the other end of the spectrum, known as the metaphysical libertarians, who maintain that claim to intentional agency cannot be sustained unless it is assumed that indeterministic causal processes pervade the action-implementation apparatus employed by the agent. The metaphysical libertarians differ among themselves on the question of whether the indeterministic causal relation exists between the series of intentional states and processes, both conscious and unconscious, and the action, making claim for what has come to be known as the event-causal view, or between the agent and the action, arguing that a sort of agent causation is at work. In this paper, I have tried to propose that certain features of both event-causal and agent-causal libertarian views need to be combined in order to provide a more defendable compatibilist account accommodating deliberative actions with deterministic causation. The ‘‘agent-executed-eventcausal libertarianism’’, the account of agency I have tried to develop here, integrates certain plausible features of the two competing accounts of libertarianism turning them into a consistent whole. I hope to show in the process that the integration of these two variants of libertarianism does not challenge what some accounts of metaphysical compatibilism propose—that there exists a broader deterministic relation between the web of mental and extra-mental components constituting the agent’s dispositional system—the agent’s beliefs, desires, short-term and long-term goals based on them, the acquired social, cultural and religious beliefs, the general and immediate and situational environment in which the agent is placed, etc. on the one hand and the decisions she makes over her lifetime on the basis of these factors. While in the ‘‘Introduction’’ the philosophically assumed anomaly between deterministic causation and the intentional act of deciding has been briefly surveyed, the second section is devoted to the task of bridging the gap between compatibilism and libertarianism. The next section of the paper turns to an analysis of folk-psychological concepts and intuitions about the effects of neurochemical processes and prior mental events on the freedom of making choices. How philosophical insights can be beneficially informed by taking into consideration folk-psychological intuitions has also been discussed, thus setting up the background for such analysis. It has been suggested in the end that support for the proposed theory of intentional agency can be found in the folk-psychological intuitions, when they are taken in the right perspective

Volcanic soils and landslides: a case study of the island of Ischia (southern Italy) and its relationship with other Campania events

Abstract. An integrated investigation was carried out on the volcanic soils involved in the landslide phenomena that occurred in 2006 at Mt. Vezzi on the island of Ischia (southern Italy). Chemical (soil pH, organic carbon content, exchangeable cations and cation exchange capacity, electrical conductivity, Na adsorption ratio and Al, Fe and Si forms), physical (particle and pore size distribution, pore structure), hydrological (soil water retention, saturated and unsaturated hydraulic conductivity), mineralogical and micromorphological analyses were carried out for three soil profiles selected in two of the main head scarps. The studied soils showed a substantial abrupt discontinuity in all the studied properties at the interface with a buried fine ash layer (namely, the 2C horizon), that was only marginally involved in the sliding surface of the landslide phenomena. When compared to the overlying horizons, 2C showed (i) fine grey ash that is almost pumice free, with the silt content increasing by 20 %; (ii) ks values 1 order of magnitude lower; (iii) a pore distribution concentrated into small (15–30 μm modal class) pores characterised by a very low percolation threshold (approximately 15–25 μm); (iv) the presence of expandable clay minerals; and (v) increasing Na content in the exchange complex. Most of these properties indicated that 2C was a lower permeability horizon compared to the overlying ones. Nevertheless, it was possible to assume this interface to be an impeding layer to vertical water fluxes only by the identification of a thin (6.5 mm) finely stratified ash layer, on top of 2C, and of the hydromorphic features (e.g. Fe / Mn concretions) within and on top of the layer. Although Mt. Vezzi's soil environment has many properties in common with those of other Campania debris-mudflows (e.g. high gradient, north-facing slope, similar forestry, and volcanic origin of the parent material), the results of this study suggest a more complex relationship between soil properties and landslides and emphasise the role of vertical discontinuities as noteworthy predisposing factors

Pleiotropic Outcomes of Glyphosate Exposure: From Organ Damage to Effects on Inflammation, Cancer, Reproduction and Development.

Glyphosate is widely used worldwide as a potent herbicide. Due to its ubiquitous use, it is detectable in air, water and foodstuffs and can accumulate in human biological fluids and tissues representing a severe human health risk. In plants, glyphosate acts as an inhibitor of the shi-kimate pathway, which is absent in vertebrates. Due to this, international scientific authorities have long‐considered glyphosate as a compound that has no or weak toxicity in humans. Howev-er, increasing evidence has highlighted the toxicity of glyphosate and its formulations in animals and human cells and tissues. Thus, despite the extension of the authorization of the use of glypho-sate in Europe until 2022, several countries have begun to take precautionary measures to reduce its diffusion. Glyphosate has been detected in urine, blood and maternal milk and has been found to induce the generation of reactive oxygen species (ROS) and several cytotoxic and genotoxic effects in vitro and in animal models directly or indirectly through its metabolite, ami-nomethylphosphonic acid (AMPA). This review aims to summarize the more relevant findings on the biological effects and underlying molecular mechanisms of glyphosate, with a particular focus on glyphosateʹs potential to induce inflammation, DNA damage and alterations in gene expression profiles as well as adverse effects on reproduction and development

Glutathione infusion before primary percutaneous coronary intervention: A randomised controlled pilot study

Objective: In the setting of reperfused ST-elevation myocardial infarction (STEMI), increased production of reactive oxygen species (ROS) contributes to reperfusion injury. Among ROS, hydrogen peroxide (H2O2) showed toxic effects on human cardiomyocytes and may induce microcirculatory impairment. Glutathione (GSH) is a water-soluble tripeptide with a potent oxidant scavenging activity. We hypothesised that the infusion of GSH before acute reoxygenation might counteract the deleterious effects of increased H2O2 generation on myocardium. Methods: Fifty consecutive patients with STEMI, scheduled to undergo primary angioplasty, were randomly assigned, before intervention, to receive an infusion of GSH (2500 mg/25 mL over 10 min), followed by drug administration at the same doses at 24, 48 and 72 hours elapsing time or placebo. Peripheral blood samples were obtained before and at the end of the procedure, as well as after 5 days. H2O2 production, 8-iso-prostaglandin F2α (PGF2α) formation, H2O2 breakdown activity (HBA) and nitric oxide (NO) bioavailability were determined. Serum cardiactroponin T (cTpT) was measured at admission and up to 5 days. Results: Following acute reperfusion, a significant reduction of H2O2 production (p=0.0015) and 8-iso-PGF2α levels (p=0.0003), as well as a significant increase in HBA (p<0.0001)and NO bioavailability (p=0.035), was found in the GSH group as compared with placebo. In treated patients, attenuated production of H2O2 persisted up to 5 days from the index procedure (p=0.009) and these changes was linked to those of the cTpT levels (r=0.41, p=0.023). Conclusion: The prophylactic and prolonged infusion of GSH seems to determine a rapid onset and persistent blunting of H2O2 generation improving myocardial cell survival. Nevertheless, a larger trial, adequately powered for evaluation of clinical endpoints, is ongoing to confirm the current finding

Probing Spin-Charge Relation by Magnetoconductance in One-Dimensional Polymer Nanofibers

Polymer nanofibers are one-dimensional organic hydrocarbon systems containing conducting polymers where the non-linear local excitations such as solitons, polarons and bipolarons formed by the electron-phonon interaction were predicted. Magnetoconductance (MC) can simultaneously probe both the spin and charge of these mobile species and identify the effects of electron-electron interactions on these nonlinear excitations. Here we report our observations of a qualitatively different MC in polyacetylene (PA) and in polyaniline (PANI) and polythiophene (PT) nanofibers. In PA the MC is essentially zero, but it is present in PANI and PT. The universal scaling behavior and the zero (finite) MC in PA (PANI and PT) nanofibers provide evidence of Coulomb interactions between spinless charged solitons (interacting polarons which carry both spin and charge)