Production of probiotic bovine salami using Lactobacillus plantarum 299v as adjunct

BACKGROUND: Five probiotic lactobacilli were tested, alone or in combination with two commercial starters, to select the most suitable strain for a probiotic bovine salami production. Lactobacillus plantarum 299v was used with both starters, to make salami according to a traditional recipe. Salami obtained by using just the starters and by spontaneous fermentation, served as control. Microbial dynamics, as well as the main physico-chemical parameters, were monitored throughout ripening. The survival of probiotic 299v was confirmed by strains' tracking by means of RAPD-PCR coupled to a culture-independent approach PCR-DGGE-based. RESULTS: The results showed a remarkable viability of the probiotic strain even after 60 days of storage. Experimental salami exhibited the same level of sensory acceptance of control salami, were hygienically safe, and characterised by pH, weight loss and microbiological loads within the ranges conventionally advocated for optimal fermented sausages. CONCLUSION: Outcomes indicate the workable possibility of using second-quality beef cuts for probiotic salami production. © 2017 Society of Chemical Industry

Spring diet of the pine marten in Sardinia, Italy

Alimentación primaveral de la marta en Cerdeña, ItaliaEl conocimiento del comportamiento de alimentación de un carnívoro es esencial para entender su ecología. La marta Martes martes se define comúnmente como un depredador generalista oportunista, porque su dieta en Europa incluye mamíferos, frutas, aves y invertebrados. A partir del análisis de las heces, hemos descrito los hábitos alimenticios en primavera y la amplitud del nicho trófico de la marta en ambiente mediterráneo en Cerdeña (Italia central). Los pequeños mamíferos (sobre todo Apodemus sylvaticus, Mus musculus y Eliomys quercinus) representan la fuente más importante de alimentación de la marta, ya que constituyen aproximadamente el 52% del volumen medio. Otra fuente importante de alimentos secundarios la constituyen los invertebrados (especialmente escarabajos y larvas de insectos) y las aves (paseriformes casi exclusivamente), mientras que los grandes mamíferos, los lagomorfos, los reptiles y las frutas están poco representados en la dieta. La composición de la dieta y el valor del índice de Levins indican que la marta en Cerdeña es un depredador especialista facultativo, con una especialización en la depredación de los micromamíferos.Knowledge of a carnivore’s foraging behaviour is essential to understand its ecology. The pine marten Martes martes is commonly defined as an opportunistic generalist predator since its diet in Europe includes mammals, fruits, birds and invertebrates. Based on faecal analyses, we evaluated spring feeding habits and trophic niche breadth of the pine marten in a Mediterranean landscape on the island of Sardinia (Central Italy). The most important source of food for the pine marten was small mammals (mainly Apodemus sylvaticus, Mus musculus and Eliomys quercinus), accounting for 52% of the percent mean volume. Important secondary foods were invertebrates (mainly beetles and insect larvae) and birds (almost exclusively passerines), whereas large mammals, lagomorphs, reptiles and fruits made little contribution to the diet. The diet composition and the Levins’ index value suggest that the pine marten in Sardinia behaves as a facultative specialist predator, with a specialization towards small mammals.Alimentación primaveral de la marta en Cerdeña, ItaliaEl conocimiento del comportamiento de alimentación de un carnívoro es esencial para entender su ecología. La marta Martes martes se define comúnmente como un depredador generalista oportunista, porque su dieta en Europa incluye mamíferos, frutas, aves y invertebrados. A partir del análisis de las heces, hemos descrito los hábitos alimenticios en primavera y la amplitud del nicho trófico de la marta en ambiente mediterráneo en Cerdeña (Italia central). Los pequeños mamíferos (sobre todo Apodemus sylvaticus, Mus musculus y Eliomys quercinus) representan la fuente más importante de alimentación de la marta, ya que constituyen aproximadamente el 52% del volumen medio. Otra fuente importante de alimentos secundarios la constituyen los invertebrados (especialmente escarabajos y larvas de insectos) y las aves (paseriformes casi exclusivamente), mientras que los grandes mamíferos, los lagomorfos, los reptiles y las frutas están poco representados en la dieta. La composición de la dieta y el valor del índice de Levins indican que la marta en Cerdeña es un depredador especialista facultativo, con una especialización en la depredación de los micromamíferos

Daily earthquake forecasts during the May-June 2012 Emilia earthquake sequence (northern Italy)

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Correlation of Static Stress Changes and Earthquake Occurrence in

A systematic analysis is made of static Coulomb stress changes and earthquake occurrence in the area of the North Aegean Sea, Greece, in order to assess the prospect of using static stress changes to construct a regional earthquake likelihood model. The earthquake data set comprises all events of magnitude M ≥ 5.2 which have occurred since 1964. This is compared to the evolving stress field due to constant tectonic loading and perturbations due to coseismic slip associated with major earthquakes (M ≥ 6.4) over the same period. The stress was resolved for sixteen fault orientation classes, covering the observed focal mechanisms of all earthquakes in the region. Analysis using error diagrams shows that earthquake occurrence is better correlated with the constant tectonic loading component of the stress field than with the total stress field changes since 1964, and that little, if any, information on earthquake occurrence is lost if only the maximum of the tectonic loading over the fault orientation classes is considered. Moreover, the information on earthquake occurrence is actually increased by taking the maximum of the evolving stress field since 1964, and of its coseismic–slip component, over the fault orientation classes. The maximum, over fault orientation classes, of linear combinations of the tectonic loading and the evolving stress field is insignificantly better correlated with earthquake occurrence than the maximum of the tectonic loading by itself. A composite stress–change variable is constructed from ordering of the maximum tectonic loading component and the maximum coseismic–slip component, in order to optimize the correlation with earthquake occurrence. The results indicate that it would be difficult to construct a time–varying earthquake likelihood model from the evolving stress field that is more informative than a time–invariant model based on the constant tectonic loading

Axessibility: a LaTeX Package for Mathematical Formulae Accessibility in PDF Documents

Accessing mathematical formulae within digital documents is challenging for blind people. In particular, document formats designed for printing, such as PDF, structure math content for visual access only. While accessibility features exist to present PDF content non-visually, formulae support is limited to providing replacement text that can be read by a screen reader or displayed on a braille bar. However, the operation of inserting replacement text is left to document authors, who rarely provide such content. Furthermore, at best, description of the formulae are provided. Thus, conveying detailed understanding of complex formulae is nearly impossible. In this contribution we report our ongoing research on Axessibility, a LATEX package framework that automates the process of making mathematical formulae accessible by providing the formulae LATEX code as PDF replacement text. Axessibility is coupled with external scripts to automate its integration in existing documents, expand user shorthand macros to standard LATEX representation, and custom screen reader dictionaries that improve formulae reading on screen readers

Clinical course and features of persistent polyclonal B-cell lymphocytosis with BCL-6 amplification during pregnancy

Background: Persistent polyclonal B-cell lymphocytosis is a rare nonmalignant disorder characterized by mild persistent lymphocyte proliferation with possible evolution to aggressive lymphoma. Its biology is not well known, but it is characterized by a specific immunophenotype with rearrangement of the BCL-2/IGH gene, whereas amplification of the BCL-6 gene has rarely been reported. Given the paucity of reports, it has been hypothesized that this disorder is associated with poor pregnancy outcomes. Case report: To our knowledge, only two successful pregnancies have been described in women with this condition. We report the third successful pregnancy in a patient with PPBL and the first with amplification of the BCL-6 gene. Conclusions: PPBL is still a poorly understood clinical condition with insufficient data to demonstrate an adverse effect on pregnancy. The role of BCL-6 dysregulation in the pathogenesis of PPBL and its prognostic significance are still unknown. Evolution into aggressive clonal lymphoproliferative disorders is possible and prolonged hematologic follow-up is warranted in patients with this rare clinical disorder