Effect of weak magnetic field on polariton-electron scattering in semiconductor microcavities

We theoretically calculate the polariton linewidth associated with the polariton-electron scattering in a microcavity in presence of a magnetic field perpendicular to the microcavity plane. It is shown that the polariton linewidth oscillates as a function of the magnetic field magnitude and the polariton-electron scattering rate can be not only decreased but also increased by the magnetic field. The possible applications of such an effect are discussed.Comment: LaTex, 6 pages, 3 figure

Organização do tráfego de veículos com base no monitoramento ambiental da bacia aérea

Modern scientific knowledge of a problem of environmental pollution by the motor transport is based on idea of negative impact of all transport complex, and not just separate objects of transport. The problem of environmental pollution by objects of a motor transportation complex is aggravated with rapid growth of vehicular fleet. In total with insufficient development of a street road network and low level of quality of management of motor transportation streams it leads to emergence of a critical ecological situation in the cities. Along with the progressing deterioration of atmospheric air also other environmental problems connected with production of automotive vehicles, spare parts and operational materials, operation, service, storage and vehicle scrappage become aggravated.In the circumstances restriction of emissions of pollutants from motor transportation streams taking into account pollution of the air basin with stationary sources has high degree of relevance. At realization of actions of this direction also projection of quality of the air basin on the basis of environmental monitoring and imitating modeling of motor transportation streams is significant objective current.Establishment of quotas for emissions of pollutants from motor transportation streams taking into account the polluting background and achievement of the established quotas at the expense of the organization of the movement of cars is represented to one of effective ways of  permission of a problem situation. The offered way of a predetermined development of the technique of the system analysis defining the sequence of stages of carrying out the analysis and methods of their performance, uniting mathematical modeling and programming, techniques of sampling of air, the laboratory and tool analysis and statistical processing of results of an experiment. In this regard, the way of restriction of emissions of pollutants from motor transportation streams by introduction of quotas for which realization the algorithm, the special software and a settlement and tool technique of environmental monitoring of motor transportation streams is offered is developed.El conocimiento científico moderno de un problema de contaminación ambiental por el transporte de automóviles se basa en la idea del impacto negativo de todos los complejos de transporte, y no solo de los objetos de transporte separados. El problema de la contaminación ambiental por objetos de un complejo de transporte de motor se ve agravado con el rápido crecimiento de la flota vehícular. En total, con un desarrollo insuficiente de una red vial de calles y un bajo nivel de calidad de la gestión de las rutas de transporte motor, conduce a la emergencia de una situación ecológica crítica en las ciudades. Junto con el deterioro progresivo del aire atmosférico también se agravan otros problemas ambientales relacionados con la producción de vehículos automotores, piezas de repuesto y materiales operacionales, operación, servicio, almacenamiento y chatarra de vehículos.En estas circunstancias, la restricción de las emisiones de contaminantes de las corrientes de transporte motor teniendo en cuenta la contaminación de la cuenca atmosférica con fuentes estacionarias tiene un alto grado de relevancia. En la realización de las acciones de esta dirección también la proyección de la cualidad de la balsa de aire sobre la base de la observación del medio ambiente y la imitación del modelado de los flujos del transporte a motor es la corriente objetivo significativa.El establecimiento de cuotas para las emisiones de contaminantes de los flujos de transporte  motor teniendo en cuenta el fondo contaminante y el logro de las cuotas establecidas a expensas de la organización del movimiento de automóviles está representado por una de las formas efectivas de autorización de una situación problemática. La manera de desarrollo predeterminado de la técnica del análisis del sistema define la secuencia de etapas de realización del análisis y métodos de su desempeño, uniendo el modelado y programación matemática, técnicas de muestreo de aire, el análisis de laboratorio y herramienta y el procesamiento estadístico de resultados de un experimento. En este sentido, se desarrolla la forma de restricción de las emisiones de contaminantes de las corrientes de transporte motor mediante la introducción de cuotas para las cuales se ofrece la realización del algoritmo, el software especial y una técnica de asentamiento y herramienta de monitoreo ambiental de las corrientes de transporte motor.O conhecimento científico moderno de um problema da poluição ambiental pelo transporte motor é baseado na ideia do impacto negativo de todo o complexo de transporte, e não apenas objetos de transporte separados. O problema da poluição ambiental por objetos de um complexo de transporte motorizado é agravado com o rápido crescimento da frota de veículos. No total com o desenvolvimento insuficiente de uma rede rodoviária de rua e o baixo nível da qualidade da gestão de correntes de transporte motoras leva à emergência de uma situação ecológica crítica nas cidades. Juntamente com a deterioração progressiva do ar atmosférico, outros problemas ambientais relacionados à produção de veículos automotivos, peças sobressalentes e materiais operacionais, operação, manutenção, armazenamento e sucateamento de veículos se agravam. Nas circunstâncias a restrição de emissões de poluentes de correntes de transporte de motor que levam em conta a poluição da bacia aérea com fontes estacionárias tem o alto grau da relevância. No momento da realização de ações desta direção também a projeção da qualidade da bacia aérea com base no monitoramento ambiental e imitando a modelagem de correntes de transporte de motor é a corrente objetiva significante. O estabelecimento de cotas para emissões de poluentes de fluxos de transporte de motor que levam em conta o fundo poluindo e realização das cotas estabelecidas às custas da organização do movimento de carros é representado a um de modos efetivos de permissão de uma situação de problema. O caminho oferecido desenvolvimento predeterminado da técnica da análise de sistema definindo a seqüência de etapas de executar a análise e métodos de seu desempenho, unindo modelagem matemática e programação, técnicas de amostragem de ar, a análise de laboratório e ferramenta e processamento estatístico de resultados de um experimento. Neste sentido, o caminho de restrição de emissões de poluentes de fluxos de transporte de motor pela introdução de cotas para a qual a realização o algoritmo, o software especial e uma técnica de ajuste e instrumento de monitorização ambiental de fluxos de transporte de motor se oferece desenvolve-se

Comprehensive approach to estimation of environmental Hazards of motor transport in industrial city

© 2018, Institute of Advanced Scientific Research, Inc.. All rights reserved. In this article the questions of an estimation of an ecological danger level of motor transport in the conditions of an industrial city are considered. The theoretical and practical substantiation of the use of the integrated approach to the estimation of the parameters of the system "Traffic flow-street of industrial city" is presented. The integrated approach proposed in the article is aimed at ensuring objectivity in modelling the process of functioning of motor transport streams in the conditions of an industrial city due to better taking into account the negative ecological effect of the aggregate chemical and energy pollution. A comparative analysis of the organization of traffic in an industrial city on the basis of ecological criteria using classical and complex approaches is presented. The revealed contradictions allowed to prove the adequacy of the proposed provisions concerning the development and application of a new criterion-a complex index of environmental load, which complements the representations of the system "Traffic flow-street of industrial city"

Disease-causing mutations in BEST1 gene are associated with altered sorting of bestrophin-1 protein

Doumanov, Jordan A. et al.Mutations in BEST1 gene, encoding the bestrophin-1 (Best1) protein are associated with macular dystrophies. Best1 is predominantly expressed in the retinal pigment epithelium (RPE), and is inserted in its basolateral membrane. We investigated the cellular localization in polarized MDCKII cells of disease-associated Best1 mutant proteins to study specific sorting motifs of Best1. Real-time PCR and western blots for endogenous expression of BEST1 in MDCK cells were performed. Best1 mutant constructs were generated using site-directed mutagenesis and transfected in MDCK cells. For protein sorting, confocal microscopy studies, biotinylation assays and statistical methods for quantification of mislocalization were used. Analysis of endogenous expression of BEST1 in MDCK cells revealed the presence of BEST1 transcript but no protein. Confocal microscopy and quantitative analyses indicate that transfected normal human Best1 displays a basolateral localization in MDCK cells, while cell sorting of several Best1 mutants (Y85H, Q96R, L100R, Y227N, Y227E) was altered. In contrast to constitutively active Y227E, constitutively inactive Y227F Best1 mutant localized basolaterally similar to the normal Best1 protein. Our data suggest that at least three basolateral sorting motifs might be implicated in proper Best1 basolateral localization. In addition, non-phosphorylated tyrosine 227 could play a role for basolateral delivery. © 2013 by the authors; licensee MDPI, Basel, Switzerland.This work was supported by Agence Nationale de la Recherche (Chaire d’Excellence to Shomi S. Bhattacharya), Fondation Voir et Entendre (Young Investigator Grants to Christina Zeitz and Emeline F. Nandrot), Centre National de la Recherche Scientifique (CNRS) and Fondation Bettencourt Schueller (to Emeline F. Nandrot), Université Pierre et Marie Curie-Paris6 (Bonus Qualité Recherche to Christina Zeitz), Foundation Fighting Blindness (grant number CD-CL-0808-0466-CHNO to Isabelle Audo), Centre d’Investigation Clinique 503 recognized as a Foundation Fighting Blindness Center (grant number C-CMM-0907-0428-INSERM04), Fundacion Progreso y Salud (to Shomi S. Bhattacharya, Maria Luz Bellido Diaz, Abhay Krishna and Paloma Dominguez Gimenez), Instituto de Salud Carlos III (grant number CM06/00183 to Maria Luz Bellido Diaz) and Bulgarian National Science Fund (grant number DDVU 02/10). Additionally, the Institut de la Vision is funded by Institut National de la Santé et de la Recherche Médicale, Université Pierre et Marie Curie-Paris 6, Centre National de la Recherche Scientifique and Départment de Paris.Peer Reviewe

Comprehensive approach to estimation of environmental Hazards of motor transport in industrial city

© 2018, Institute of Advanced Scientific Research, Inc.. All rights reserved. In this article the questions of an estimation of an ecological danger level of motor transport in the conditions of an industrial city are considered. The theoretical and practical substantiation of the use of the integrated approach to the estimation of the parameters of the system "Traffic flow-street of industrial city" is presented. The integrated approach proposed in the article is aimed at ensuring objectivity in modelling the process of functioning of motor transport streams in the conditions of an industrial city due to better taking into account the negative ecological effect of the aggregate chemical and energy pollution. A comparative analysis of the organization of traffic in an industrial city on the basis of ecological criteria using classical and complex approaches is presented. The revealed contradictions allowed to prove the adequacy of the proposed provisions concerning the development and application of a new criterion-a complex index of environmental load, which complements the representations of the system "Traffic flow-street of industrial city"

Cytotoxicity of flavonoid glycosides, flavonoids and phenolic acids from Inula oculus-christi on mammalian cell lines

Herbs of the genus Inula are well known in traditional medicine. Their extracts are used as expectorants, antitussives, bactericides as well as for the treatment of lung inflammation and have shown to possess anti-inflammatory and secretolytic activity. Experimental research findings indicate the anti-tumor effect of certain components of extracts from Inula cappa and Inula britannicа and those from Inula racemosa have antimicrobial and antidiabetic activity. We have directed our efforts on investigating the effects of different extracts from Inula oculus-christi, enriched with certain groups of biologically active substances - flavonoid glycosides (A), phenolic acids (B), flavonoid glycosides and phenolic acids (C), flavonoids and phenolic acids (D). The effect of these extracts on normal (MDCK ІІ and RPE1) and carcinoma cell lines (A549 and HepG2) was evaluated. We have performed cytotoxicity study (crystal violet assay) as well as morphological analysis of changes induced by the extracts. Among the tumor cell lines HepG2 show greater sensitivity. Surprisingly extract C has no significant influence on both cancer cell lines

Condensing effect of cholesterol on hBest1/POPC and hBest1/SM Langmuir monolayers

Human bestrophin-1 protein (hBest1) is a transmembrane channel associated with the calcium-dependent transport of chloride ions in the retinal pigment epithelium as well as with the transport of glutamate and GABA in nerve cells. Interactions between hBest1, sphingomyelins, phosphatidylcholines and cholesterol are crucial for hBest1 association with cell membrane domains and its biological functions. As cholesterol plays a key role in the formation of lipid rafts, motional ordering of lipids and modeling/remodeling of the lateral membrane structure, we examined the effect of different cholesterol concentrations on the surface tension of hBest1/POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and hBest1/SM Langmuir monolayers in the presence/absence of Ca2+ ions using surface pressure measurements and Brewster angle microscopy studies. Here, we report that cholesterol: (1) has negligible condensing effect on pure hBest1 monolayers detected mainly in the presence of Ca2+ ions, and; (2) induces a condensing effect on composite hBest1/POPC and hBest1/SM monolayers. These results offer evidence for the significance of intermolecular protein–lipid interactions for the conformational dynamics of hBest1 and its biological functions as multimeric ion channel

Effects of Ca2+ ions on bestrophin-1 surface films.

Human bestrophin-1 (hBest1) is a transmembrane calcium-activated chloride channel protein - member of the bestrophin family of anion channels, predominantly expressed in the membrane of retinal pigment epithelium (RPE) cells. Mutations in the protein cause ocular diseases, named Bestrophinopathies. Here, we present the first Fourier transform infrared (FTIR) study of the secondary structure elements of hBest1, π/A isotherms and hysteresis, Brewster angle microscopy (BAM) and atomic force microscopy (AFM) visualization of the aggregation state of protein molecules dispersed as Langmuir and Langmuir-Blodgett films. The secondary structure of hBest1 consists predominantly of 310-helices (27.2%), α-helixes (16.3%), β-turns and loops (32.2%). AFM images of hBest1 suggest approximate lateral dimensions of 100×160Å and 75Å height. Binding of calcium ions (Ca2+) induces conformational changes in the protein secondary structure leading to assembly of protein molecules and changes in molecular and macro-organization of hBest1 in monolayers. These data provide basic information needed in pursuit of molecular mechanisms underlying retinal and other pathologies linked to this protein