The Molecular Pathology of Prion Diseases

Prion diseases, or transmissible spongiform encephalopathies (TSEs), are a group of invariably fatal neurodegenerative disorders. Uniquely, they may present as sporadic, inherited, or infectious forms, all of which involve conversion of the normal cellular prion protein (PrPC) into a pathogenic likeness of itself (PrPSc). Formation of neurotoxic PrPSc and/or loss of the normal function of native PrPC result in activation of cellular pathways ultimately leading to neuronal death. Prion diseases can affect both humans and animals, with scrapie of sheep, bovine spongiform encephalopathy (BSE), and Creutzfeldt-Jakob disease being the most notable. This review is intended to provide an overview of the salient scientific discoveries in prion research, mainly from a molecular perspective. Further, some of the major outstanding questions in prion science are highlighted. Prion research is having a profound impact on modern medicine, and strategies for prevention and treatment of these disorders may also find application in the more common neurodegenerative diseases.peer-reviewe

Emergence and Adult Biology of \u3ci\u3eAgrilus Difficilis\u3c/i\u3e (Coleoptera: Buprestidae), a Pest of Honeylocust, \u3ci\u3eGleditsia Triacanthos\u3c/i\u3e

Emergence and adult biology of Agrilus difficilis were examined in relation to its host Gleditsia triacanthos. began as early as 5 June in 1982 and completed as late as 22 July in 1983. Females lived significantly longer, 48 days, than males, 29 days. Average fecundity was one egg per day during a 36-day oviposition period

Laboratory Rearing of \u3ci\u3eLycaeides Melissa Samuelis\u3c/i\u3e (Lepidoptera: Lycaenidae), An Endangered Butterfly in Michigan

The Karner blue butterfly (Lycaeides melissa samuelis) is listed as a federally endangered species in the United States. It occurs in oak savanna and pine barren habitats from eastern Minnesota to New Hampshire. In 1994, we successfully reared Karner blue larvae under controlled laboratory conditions for experimental purposes, and report on those rearing methods here. We collected 20 female Karner blue adults of the spring generation from two areas in Michigan, and housed them in cages in an environmental chamber at 240 -26°C for 5 days. The female butterflies produced 154 eggs, of which 72 hatched in an average of 4.5 days, and 68 first instars survived. Eggs, larvae and pupae were kept in a growth chamber at 24°C. Developmental time from egg to adult averaged 26 days; the average duration of each instar ranged from 3 to 4 days, and the average pupal duration was 8 days. Thirty three lab- oratory-reared Karner blue larvae successfully completed the 4 instars, and were released as adults into maternal collection sites. Laboratory rearing may be a viable means of providing Karner blue individuals for reintroduction into areas where the species is extirpated, for supplementation of small populations, or for research. Ultimately, such methods may become an integral part in the recovery of this and other rare invertebrate species

New Reports of Exotic and Native Ambrosia and Bark Beetle Species (Coleoptera: Curculionidae: Scolytinae) From Ohio

In a 2007 survey of ambrosia and bark beetles (Coleoptera: Curculionidae: Scolytinae) along a transect in northeastern Ohio, we collected six exotic and three native species not previously reported from the state. These species include the exotic ambrosia beetles Ambrosiodmus rubricollis (Eichhoff), Dryoxylon onoharaensum (Murayama), Euwallacea validus (Eichhoff), Xyleborus californicus Wood, Xyleborus pelliculosusEichhoff, and Xylosandrus crassiusculus (Motschulsky). The native ambrosia beetle Corthylus columbianus Hopkins, and the native bark beetles Dryocoetes autographus (Ratzeburg) and Hylastes tenuis Eichhoff are also reported from Ohio for the first time. Our study suggests a northward range expansion for five of the six exotic species including, X. crassiusculus, which is an important pest of nursery and orchard crops in the southeastern United States

Susceptibility of the Endangered Karner Blue Butterfly (Lepidoptera: Lycaenidae) to \u3ci\u3eBacillus Thuringiensis\u3c/i\u3e Var. \u3ci\u3eKurstaki\u3c/i\u3e Used for Gypsy Moth Suppression in Michigan

We investigated the phenological and physiological susceptibility of the endangered Karner blue butterfly (Lycaeides melissa samuelis) to Bacillus thuringiensis var. kurstaki (Bt), a product widely used for gypsy moth (Lymantria dispar) suppression in Michigan and other infested states. We monitored phenology of the bivoltine Karner blue in two regions of Michigan from 1993 to 1995 to determine if larval stages overlapped temporally with the period of Bt application for gypsy moth suppression. Karner blue larvae of the spring generation were found during the period that Bt was applied in nearby areas in 1993 only. However, spring-generation adults or newly laid eggs were observed up to 11 days before applications in 1994 and 1995. Since Karner blue eggs develop within one week, summer-generation larvae were most likely present during or shortly after 1994 and 1995 Bt application periods. These larvae would have been at risk, assuming Bt persistence of 4 to 6 days. Physiological susceptibility of Karner blue larvae to Bt was determined in a laboratory bioassay. Larvae were reared on wild lupine (Lupinus perennis) foliage that was untreated, or sprayed with Bt formulations at rates of 30-37 or 90 BIU/ha. A similar bioassay with second instar gypsy moth larvae on similarly treated white oak (Quercus alba) foliage was conducted concurrently. Karner blue survival was 100%, 27% and 14% on control, low and high Bt treatments, respectively. Early and late Karner blue instars were equally susceptible to Bt. Survival of gypsy moth was 80%, 33% and 5% on control, low and high Bt treatments, respectively, and did not differ significantly from Karner blue survival. We conclude that Karner blue is both phenologically and physiologically susceptible to Bt used for gypsy moth suppression, although the larval generation at risk and extent of phenological overlap may vary from year to year

Implications of Climate Change for Agricultural Pest Management

Termination ReportThe University Archives has determined that this item is of continuing value to OSU's history.The thermal requirements of crops and insect pests differ significantly; their differential thermal requirements will govern how climate change impacts pest management needs and practices. To investigate the impact of climate change on pest management and the implications for farming communities, we have connected EPIC, a field-scale crop management model, with a temperature-driven model of insect population development (GILSM). EPIC was used to model the corn and soybean rotation common in the Midwest, and GILSM was programmed to model the growth of nine insect pests of corn and/or soybeans. Output from the model system was input to a geographic information system covering the eight-state corn-belt (Indiana, Illinois, Iowa, Kansas, Kentucky, Missouri, Nebraska and Ohio). The models were driven using GFDL-CM2 climate scenario data developed for the period 1901-2100 as part of NOA and IPCC investigations of global climate change. Over the past 100 years, crop productivity has increased several fold as a result of improved cultivation methods, fertilizers and pesticides, and plant breeding. In order to remove the confounding of changes in technology and possible climate change over the past century and unknown changes to come in the next century, a standard crop production protocol was used from 1901-2100. Using the crop production practices used most commonly in 2000 (the midpoint of the period), EPIC/GILSM was run for four 50 year periods (1901-1950, 1951-2000, 2001-2050 and 2051-2100) and the changes in crop yield and insect abundance were examined between periods. Nine insect pests found throughout the region were modeled to examine the range of likely responses of insect pests to climate change and the possible change in crop protection needs over the next 100 years. As expected, the model predicted little or no change between the first and second periods. For most species, change was maximum in the third period and less during the fourth period. In every case the isoclines for pest population density and crop productivity moved northwards, but at different rates, resulting in the isoclines for crop damage increasing as they moved northward. In two cases the pest abundance declined locally even as productivity increased, resulting in a net increase in productivity. No allowance was made for changes in agronomic practices or improvements in breeding pest resistance, both of which have helped to improve productivity over the past century.SEEDS-The OARDC Research Enhancement Competitive Grants Program: Interdisciplinary Team Research Competitio

Soil Management Regimes for Plant Health Care and Integrated Pest Management Programs in Ornamental Landscapes

Author Institution (Herms and Stinner): Department of Entomology, The Ohio State University; Author Institution (Hoitink): Department of Plant Pathology, The Ohio State University; Author Institution (Rose): Department of Horticulture and Crop Science, The Ohio State Universit

Long-term in vivo imaging of fibrillar tau in the retina of P301S transgenic mice.

Tauopathies are widespread neurodegenerative disorders characterised by the intracellular accumulation of hyperphosphorylated tau. Especially in Alzheimer's disease, pathological alterations in the retina are discussed as potential biomarkers to improve early diagnosis of the disease. Using mice expressing human mutant P301S tau, we demonstrate for the first time a straightforward optical approach for the in vivo detection of fibrillar tau in the retina. Longitudinal examinations of individual animals revealed the fate of single cells containing fibrillar tau and the progression of tau pathology over several months. This technique is most suitable to monitor therapeutic interventions aimed at reducing the accumulation of fibrillar tau. In order to evaluate if this approach can be translated to human diagnosis, we tried to detect fibrillar protein aggregates in the post-mortem retinas of patients that had suffered from Alzheimer's disease or Progressive Supranuclear Palsy. Even though we could detect hyperphosphorylated tau, we did not observe any fibrillar tau or Aß aggregates. In contradiction to previous studies, our observations do not support the notion that Aβ or tau in the retina are of diagnostic value in Alzheimer's disease

Vertically Stratified Ash-Limb Beetle Fauna in Northern Ohio

To better understand the diversity and ecology of indigenous arthropods at risk from the invasive emerald ash borer (Agrilus planipennis Fairmaire) in North American forests, saproxylic beetles (Insecta: Coleoptera) were reared from ash (Fraxinus sp.) limbs suspended in the canopy, ~10–17 m above the ground, and from those placed on the ground in a mature mixed hardwood forest. In total, 209 specimens from 9 families and 18 species were collected from 30.0 m2 of limbs. The generalist cerambycid Neoclytus acuminatus (Fabricius) was the most commonly captured taxon, followed by an assemblage of four exotic ambrosia beetles dominated by Xylosandrus crassiusculus (Motschulsky). Two species largely or entirely restricted to ash, the buprestid Agrilus subcinctus Gory and the curculionid Hylesinus aculeatus (Say), were collected as well. Although there were no differences in beetle richness, abundance, or density between limb positions, community composition differed significantly. This can be largely attributed to phloem and wood-feeding species (i.e., Cerambycidae and Buprestidae) being more common in the suspended limbs and ambrosia beetles being more numerous on the forest floor. Possible explanations for these patterns are discussed

Induced seismicity in pressurised single fractures: a numerical approach

The exploration and exploitation of deep geothermal reservoirs has significantly increased during the last years. These reservoirs use heat exchange to produce heat or electricity. The so-called Enhanced Geothermal Systems (EGS) are characterized by a stimulation phase that aims to increase fluid flow and heat transfer between wells by increasing the permeability and transitivity of the reservoir. This is achieved by injecting high-pressure fluids (normally water) in order to increase the apertures of existing fractures, enhancing their sliding and/or generating new ones. However, this technique induces low-magnitude seismicity that occasionally results in damage at the Earth's surface. Numerical simulations able to reproduce the hydro-thermo-mechanical behaviour of geological reservoirs are an essential tool for the evaluation and forecasting of induced seismicity in such systems. In this study, the numerical code CFRAC is used to systematically evaluate how the orientation of faults with respect to the stress field influences seismicity, the injection rate and the fracture sliding behaviour