26 research outputs found
Systemic availability and metabolism of colonic-derived short-chain fatty acids in healthy subjects: a stable isotope study
The short-chain fatty acids (SCFAs), acetate, propionate and butyrate, are bacterial metabolites that mediate the interaction between the diet, the microbiota and the host. In the present study, the systemic availability of SCFAs and their incorporation into biologically relevant molecules was quantified. Known amounts of 13C-labelled acetate, propionate and butyrate were introduced in the colon of 12 healthy subjects using colon delivery capsules and plasma levels of 13C-SCFAs 13C-glucose, 13C-cholesterol and 13C-fatty acids were measured. The butyrate-producing capacity of the intestinal microbiota was also quantified. Systemic availability of colonic-administered acetate, propionate and butyrate was 36%, 9% and 2%, respectively. Conversion of acetate into butyrate (24%) was the most prevalent interconversion by the colonic microbiota and was not related to the butyrate-producing capacity in the faecal samples. Less than 1% of administered acetate was incorporated into cholesterol and <15% in fatty acids. On average, 6% of colonic propionate was incorporated into glucose. The SCFAs were mainly excreted via the lungs after oxidation to 13CO2, whereas less than 0.05% of the SCFAs were excreted into urine. These results will allow future evaluation and quantification of SCFA production from 13C-labelled fibres in the human colon by measurement of 13C-labelled SCFA concentrations in blood
Oplossingen voor bodemmoeheid in de fruitteelt
De herinplant in de fruitteelt is alleen mogelijk als de bodemmoeheid wordt aangepakt. Alternatieve methoden van grondontsmetting waren tot nu toe in de meeste gevallen minder effectief of in vergelijking met chemische grondontsmetting veel duurder. In de biologische fruitteelt is chemische ontsmetting geen optie. Uit recent onderzoek blijken een aantal biologische behandelingen geschikt om bodemmoeheid te bestrijden: zwarte braak, Tagetes (Afrikaantjes) en biologische grondontsmetting (BGO). Biofumigatie en compost bleken minder effectief of geschikt
Food Matrix Design for Effective Lactic Acid Bacteria Delivery
International audienceThe range of foods featuring lactic acid bacteria (LAB) with potential associated health benefits has expanded over the years from traditional dairy products to meat, cereals, vegetables and fruits, chocolate, etc. All these new carriers need to be compared for their efficacy to protect, carry, and deliver LAB, but because of their profusion and the diversity of methods this remains difficult. This review points out the advantages and disadvantages of the main food matrix types, and an additional distinction between dairy and nondairy foods is made. The food matrix impact on LAB viability during food manufacturing, storage, and digestion is also discussed. The authors propose an ideal hypothetical food matrix that includes structural and physicochemical characteristics such as pH, water activity, and buffering capacities, all of which need to be taken into account when performing LAB food matrix design. Guidelines are finally provided to optimize food matrix design in terms of effective LAB delivery
Molecular and Morphological Aspects of Annealing-Induced Stabilization of Starch Crystallites
A unique series of potato (mutant) starches with highly
different
amylopectin/amylose (AP/AM) ratios was annealed in excess water at
stepwise increasing temperatures to increase the starch melting (or
gelatinization) temperatures in aqueous suspensions. Small-angle X-ray
scattering (SAXS) experiments revealed that the lamellar starch crystals
gain stability upon annealing via thickening for high-AM starch, whereas
the crystal surface energy decreases for AM-free starch. In starches
with intermediate AP/AM ratio, both mechanisms occur, but the surface
energy reduction mechanism prevails. Crystal thickening seems to be
associated with the cocrystallization of AM with AP, leading to very
disordered nanomorphologies for which a new SAXS data interpretation
scheme needed to be developed. Annealing affects neither the crystal
internal structure nor the spherulitic morphology on a micrometer
length scale
Distribution of Minerals in Wheat Grains (<i>Triticum aestivum</i> L.) and in Roller Milling Fractions Affected by Pearling
The
distribution of minerals in (pearled) wheat grains was measured
by synchrotron X-ray fluorescence, and the impact of pearling (0,
3, 6, 9, and 12% by weight) on the mineral composition of flour, shorts,
and bran was identified by ICP-MS. The xylem mobile elements (Mn,
Si, Ca, and Sr) dominated in the outermost bran layers, while the
phloem mobile elements (K, Mg, P, Fe, Zn, and Cu) were more concentrated
in the aleurone. Pearling lowered the concentrations of xylem mobile
elements and increased the concentrations of most phloem mobile elements
in the pearled grains. Molybdenum, Cd, and especially Se were more
evenly distributed, and pearling affected their concentrations in
milling products less. Pearling (3%) increased the concentration of
several nutrients (P, Zn, Cu) in the flour because the bran fractions
reaching the flour are enriched in aleurone. The correlations of concentrations
of Mg, Fe, Zn, and Cu with that of P suggested their association with
phytate