Botulisme canin : Première souche isolée en France

Vallée A., Kréguer A., Eyquem A. Botulisme canin : Première souche isolée en France. In: Bulletin de l'Académie Vétérinaire de France tome 109 n°8, 1956. pp. 419-423

Cerebrospinal fluid neurogranin in an inducible mouse model of neurodegeneration: A translatable marker of synaptic degeneration

Synapse impairment is thought to be an early event in Alzheimer's disease (AD); dysfunction and loss of synapses are linked to cognitive symptoms that precede neuronal loss and neurodegeneration. Neurogranin (Ng) is a somatodendritic protein that has been shown to be reduced in brain tissue but increased in the cerebrospinal fluid (CSF) of AD patients compared to age-matched controls. High levels of CSF Ng have been shown to reflect a more rapid AD progression. To gauge the translational value of Ng as a biomarker, we developed a new, highly sensitive, digital enzyme-linked immunosorbent assay (ELISA) on the Simoa platform to measure Ng in both mouse and human CSF. We investigated and confirmed that Ng levels are increased in the CSF of patients with AD compared to controls. In addition, we explored how Ng is altered in the brain and CSF of transgenic mice that display progressive neuronal loss and synaptic degeneration following the induction of p25 overexpression. In this model, we found that Ng levels increased in CSF when neurodegeneration was induced, peaking after 2 weeks, while they decreased in brain. Our data suggest that CSF Ng is a biomarker of synaptic degeneration with translational value

The CD28-Transmembrane Domain Mediates Chimeric Antigen Receptor Heterodimerization With CD28.

Anti-CD19 chimeric antigen receptor (CD19-CAR)-engineered T cells are approved therapeutics for malignancies. The impact of the hinge domain (HD) and the transmembrane domain (TMD) between the extracellular antigen-targeting CARs and the intracellular signaling modalities of CARs has not been systemically studied. In this study, a series of 19-CARs differing only by their HD (CD8, CD28, or IgG <sub>4</sub> ) and TMD (CD8 or CD28) was generated. CARs containing a CD28-TMD, but not a CD8-TMD, formed heterodimers with the endogenous CD28 in human T cells, as shown by co-immunoprecipitation and CAR-dependent proliferation of anti-CD28 stimulation. This dimerization was dependent on polar amino acids in the CD28-TMD and was more efficient with CARs containing CD28 or CD8 HD than IgG <sub>4</sub> -HD. The CD28-CAR heterodimers did not respond to CD80 and CD86 stimulation but had a significantly reduced CD28 cell-surface expression. These data unveiled a fundamental difference between CD28-TMD and CD8-TMD and indicated that CD28-TMD can modulate CAR T-cell activities by engaging endogenous partners

Core binding factors are necessary for natural killer cell development, and cooperate with Notch signaling during T cell specification

CBF{beta} is the non-DNA binding subunit of the core binding factors (CBFs). Mice with reduced CBF{beta} levels display profound, early defects in T but not B cell development. Here we show that CBF{beta} is also required at very early stages of natural killer (NK) cell development. We also demonstrate that T cell development aborts during specification, as the expression of Gata3 and Tcf7, which encode key regulators of T lineage specification, is substantially reduced, as are functional thymic progenitors. Constitutively active Notch or IL-7 signaling cannot restore T cell expansion or differentiation of CBF{beta} insufficient cells, nor can overexpression of Runx1 or CBF{beta} overcome a lack of Notch signaling. Therefore the ability of the prethymic cell to respond appropriately to Notch is dependent on CBF{beta}, and both signals converge to activate the T cell developmental program

Concurso Bienal 1975 - 1976

Contenido Casas entre mediaderos Simón Bolivar, Santiago - Conjunto Habitacional Siete Hermanas Viña del Mar - Industria Nacional del Cemento "Inacesa" Antofagasta - Central de Adquisiciones Automotrices Santiago - Estudio preinversional de vivienda y desarrollo urbano para Iquique "Cedur" - Planeamiento del equipo escolar "Centro Metropolitano" de Concepción - Sociedad de stablecimientos Educacionales - Nuede Arquitectura y Urbanismo de la Universidad de Chile - Vivienda popular en Santiago de Chile - Elemento estructural Feria Municipal de la Vega Santiago - Hotel Internacional Rocas de Santo Domingo

Cellular Therapy with Engineered T Cells, Efficacy and Side Effects

The cellular basis of cancer immune surveillance, already hypothesized in ancient times, was only demonstrated with the advent of HSCT. Indeed, the discovery of the nature of GVHD and its antileukemic effects (Weiden et al

A propos de l'immuno-pathologie humorale chez le chien

Research of the autoimmune reaction in 166 subjects of habitual population in a veterinary consultation.La recherche des réactions auto-immunes positives. Sur 166 sujets d’une population habituelle d'une consultation vétérinaire a permis de constater leur faible importance. Il n'a pas été observé de différence avec le sexe et avec l'âge.Eyquem A., Groulade Paul. A propos de l'immuno-pathologie humorale chez le chien. In: Bulletin de l'Académie Vétérinaire de France tome 131 n°2, 1978. pp. 247-251