Pramipexole protective effect on rotenone induced neurotoxicity in mice

Introduction: 
Pramipexole is a new dopaminergic drug which has been approved for PD treatment. However, we tried to find a new capacity for this drug rather than symptomatic effect. 

Materials and Methods: 
A chronic rotenone model with daily oral dose of 30mg/kg was induced in mice. Pramipexole was tried in a new approach where the treatment began in the middle of rotenone course with oral dose 1mg/kg/day of pramipexole. 

Results: 
Further analysis of behavioral tests and immunohistochemistry revealed success of pramipexole in improving the rotenone intoxicated mice. 

Conclusion: 
These results showed possible beneficial effects of pramipexole against rotenone-induced neurotoxicity

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Triacetic acid lactone in the synthesis of heterocyclic compounds

This work was financially supported by the Russian Science Foundation (Grant no.17-73-20070)

DNA Fingerprinting, Chemical Composition, Antitumor and Antimicrobial Activities of the Essential Oils and Extractives of four Annona Species from Egypt

The leaf essential oils of four members of the Annonaceae grown in Egypt (namely; Annona cherimola, A. squamosa, A. muricata and A. glabra) have been obtained by hydrodistillation and analyzed by GC-MS in order to compare and contrast the volatile chemical compositions of these species.  The essential oils were screened for in-vitro cytotoxic activity against breast cancer (MCF-7), colon cancer (CACO) and liver cancer (HEPG2) cell lines and antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeuroginosa, Aspergillus flavus and Candida albicans. beta-elemene (25.02%), beta-caryophyllene (37.11%), bicycloelemene (23.58%) and and beta-gurjunene (42.49%), were the major constituents of Annona cherimola, A. squamosa, A. muricata and A. glabra, respectively. Ethanol extracts showed highly significant cytotoxic activities (low IC50) much more than results displayed by essential oils on breast (MCF-7), colon (CACO) and liver (HEPG2) carcinoma cell lines. Relative to breast carcinoma cell line (MCF-7), The IC50 values of ethanol extracts were 3.43, 3.89 and 4.34 ?g/ml for A. cherimola, A. squamosa and A. muricata ethanol extractives respectively. While colon carcinoma cell line (CACO) displayed IC50 values 2.82, 2.97, 3.58 and 3.89 ?g/ml for A. muricata, A. cherimola, A. glabra and A. squamosa, respectively. Liver carcinoma cell line (HEPG2) exhibited IC50 valus of 3.12, 3.43 and 3.73 for A. squamosa, A. muricata and A. cherimola, respectively. Three of the four leaf essential oils showed notable invitro cytotoxic activity. Essential oils of A. glabra, A. muricata and A. squamosa showed moderate cytotoxic activities with IC50 values ranging from 12.35 to 24.21?g/ml. While the essential oils of A. cherimola showed IC50 values ranging from 7.67 to 9.22?g/ml. Leaf oils and ethanol extractives showed appreciable antibmicobial activity with variable MIC ranging from 30 to 315µg/ml. These findings suggest that A. cherimola essential oil and ethanol extract have great potential as a natural medicine for cancers and microbial infections. Keywords:Annona cherimola; A. squamosa;, A. muricata ; A. glabra;   Annonaceae;   DNA, essential   oil   composition; cytotoxicity; antimicobia

EFFECT OF SUGAR BEET MOLASSES AND Fe-EDHHA ON TOMATO PLANTS GROWN UNDER SALINE WATER IRRIGATION CONDITION

Salinity is a major limiting factor for tomato crop growth and productivity especially in arid and semi arid lands region. Therefore this study was conducted to study the effect of applying sugar beet molasses, priming tomato seedling in saline water and Fe-EDHHA on mitigating salinity negative effects on tomato (Lycopersicon esculentum L.)  hybrid super strain B (salinity sensitive hybrid). Seedlings of tomato hybrid were transplanted on April 4th in both seasons 2014 and 2015 in pots contains washed sandy soil, and irrigated with saline water with EC of  2000, 3000 and 4000 ppm. Beet molasses and Fe – EDHHA were applied at  rates of 200 and 300 kg / fed. for beet molasses and 3 and 4 kg / fed. for Fe – EDHHA. Priming tomato seedlings in saline water treatment was applied at 5 and 6 dS/m for 24 hours.  Data showed that all plant growth aspects such as plant height, leaf area, plant fresh and dry weights were improved under beet molasses, Fe and priming tomato seedlings in saline water treatments compared to none treated plants (control). Yield parameters also followed the same trend. Among treatments, beet molasses at a rate of 200 kg / fed. recorded the highest significant effect in mitigating salinity negative effects. It could be concluded that beet molasses, priming tomato seedling in saline water and Fe – EDHHA treatments were more effective and efficient in mitigating salinity stress on tomato plants

DNA Fingerprinting, Chemical Composition, Antitumor and Antimicrobial Activities of the Essential Oils and Extractives of four Annona Species from Egypt

The leaf essential oils of four members of the Annonaceae grown in Egypt (namely; Annona cherimola, A. squamosa, A. muricata and A. glabra) have been obtained by hydrodistillation and analyzed by GC-MS in order to compare and contrast the volatile chemical compositions of these species.  The essential oils were screened for in-vitro cytotoxic activity against breast cancer (MCF-7), colon cancer (CACO) and liver cancer (HEPG2) cell lines and antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeuroginosa, Aspergillus flavus and Candida albicans. beta-elemene (25.02%), beta-caryophyllene (37.11%), bicycloelemene (23.58%) and and beta-gurjunene (42.49%), were the major constituents of Annona cherimola, A. squamosa, A. muricata and A. glabra, respectively. Ethanol extracts showed highly significant cytotoxic activities (low IC50) much more than results displayed by essential oils on breast (MCF-7), colon (CACO) and liver (HEPG2) carcinoma cell lines. Relative to breast carcinoma cell line (MCF-7), The IC50 values of ethanol extracts were 3.43, 3.89 and 4.34 ?g/ml for A. cherimola, A. squamosa and A. muricata ethanol extractives respectively. While colon carcinoma cell line (CACO) displayed IC50 values 2.82, 2.97, 3.58 and 3.89 ?g/ml for A. muricata, A. cherimola, A. glabra and A. squamosa, respectively. Liver carcinoma cell line (HEPG2) exhibited IC50 valus of 3.12, 3.43 and 3.73 for A. squamosa, A. muricata and A. cherimola, respectively. Three of the four leaf essential oils showed notable invitro cytotoxic activity. Essential oils of A. glabra, A. muricata and A. squamosa showed moderate cytotoxic activities with IC50 values ranging from 12.35 to 24.21?g/ml. While the essential oils of A. cherimola showed IC50 values ranging from 7.67 to 9.22?g/ml. Leaf oils and ethanol extractives showed appreciable antibmicobial activity with variable MIC ranging from 30 to 315µg/ml. These findings suggest that A. cherimola essential oil and ethanol extract have great potential as a natural medicine for cancers and microbial infections. Keywords:Annona cherimola; A. squamosa;, A. muricata ; A. glabra;   Annonaceae;   DNA, essential   oil   composition; cytotoxicity; antimicobia

Bio-based Triacetic Acid Lactone in the Synthesis of Heterocycles Via the Enaminone Formation

This work was financially supported by the Russian Science Foundation (grant № 18-13-00186)