Pengaruh Pemberian Tepung Magot (Hermetia Illucens) Dalam Pakan Buatan Terhadap Pertumbuhan, Efisiensi Pakan Dan Kelangsungan Hidup Benih Ikan Mas (Cyprinus Carpio L.) [the Feeding Effect of Maggot Meal (Hermetia Illucens) in Artificial Feed on Growth, Feed Efficiency and Survival Rate of Common Carp Juvenile (Cyprinus Carpio L.) ]

Carp (C. carpio L.) is one commodity of fresh water fishery sector continues to grow rapidly in Indonesia. One of the factors that determine the success of aquaculture is the availability of sufficient feed, both in quantity and quality. The aim of this research was to find out optimum percentage of feeding maggot meal in artificial feed on growth, efficiency of feed and survival rate of common carp juvenile. The treatment of this researh was feeding maggot meal in artificial feed i.e. 0%, 5%, 10%, 15%, and 20%. The design of this research was Completely Randomized Design. Data analysis used Analysis of Variance (ANOVA) to know the effect of the treatment and the difference among the treatment used Duncan's Multiple Range Test (DMRT). The result of this research showed that the growth, efficiency of feed and survival rate of common carp that given maggot meal in artificial feed was not significantly (P>0,05). Water quality on culture medium, temperature, pH, dissolved oxygen, and ammonia was 2832oC, 7-8, 5 mg/L and 0,02-0,05mg/L respectively

Fast-resorbable antibiotic-loaded hydrogel coating to reduce post-surgical infection after internal osteosynthesis : a multicenter randomized controlled trial

BACKGROUND: Infection is one of the main reasons for failure of orthopedic implants. Antibacterial coatings may prevent bacterial adhesion and biofilm formation, according to various preclinical studies. The aim of the present study is to report the first clinical trial on an antibiotic-loaded fast-resorbable hydrogel coating (Defensive Antibacterial Coating, DAC\uae) to prevent surgical site infection, in patients undergoing internal osteosynthesis for closed fractures. MATERIALS AND METHODS: In this multicenter randomized controlled prospective study, a total of 256 patients in five European orthopedic centers who were scheduled to receive osteosynthesis for a closed fracture, were randomly assigned to receive antibiotic-loaded DAC or to a control group (without coating). Pre- and postoperative assessment of laboratory tests, wound healing, clinical scores and X-rays were performed at fixed time intervals. RESULTS: Overall, 253 patients were available with a mean follow-up of 18.1 \ub1 4.5 months (range 12-30). On average, wound healing, clinical scores, laboratory tests and radiographic findings did not show any significant difference between the two groups. Six surgical site infections (4.6%) were observed in the control group compared to none in the treated group (P < 0.03). No local or systemic side-effects related to the DAC hydrogel product were observed and no detectable interference with bone healing was noted. CONCLUSIONS: The use of a fast-resorbable antibiotic-loaded hydrogel implant coating provides a reduced rate of post-surgical site infections after internal osteosynthesis for closed fractures, without any detectable adverse event or side-effects. LEVEL OF EVIDENCE: 2

Expression of the Serpin Serine Protease Inhibitor 6 Protects Dendritic Cells from Cytotoxic T Lymphocyte–Induced Apoptosis: Differential Modulation by T Helper Type 1 and Type 2 Cells

Dendritic cells (DCs) play a central role in the immune system as they drive activation of T lymphocytes by cognate interactions. However, as DCs express high levels of major histocompatibility complex class I, this intimate contact may also result in elimination of DCs by activated cytotoxic T lymphocytes (CTLs) and thereby limit induction of immunity. We show here that immature DCs are indeed susceptible to CTL-induced killing, but become resistant upon maturation with anti-CD40 or lipopolysaccharide. Protection is achieved by expression of serine protease inhibitor (SPI)-6, a member of the serpin family that specifically inactivates granzyme B and thereby blocks CTL-induced apoptosis. Anti-CD40 and LPS-induced SPI-6 expression is sustained for long periods of time, suggesting a role for SPI-6 in the longevity of DCs. Importantly, T helper 1 cells, which mature DCs and boost CTL immunity, induce SPI-6 expression and subsequent DC resistance. In contrast, T helper 2 cells neither induce SPI-6 nor convey protection, despite the fact that they trigger DC maturation with comparable efficiency. Our data identify SPI-6 as a novel marker for DC function, which protects DCs against CTL-induced apoptosis

Maturation of monocyte-derived dendritic cells with Toll-like receptor 3 and 7/8 ligands combined with prostaglandin E2 results in high interleukin-12 production and cell migration

Dendritic cells (DC) are professional antigen-presenting cells of the immune system that play a key role in regulating T cell-based immunity. In vivo, the capacity of DC to activate T cells depends on their ability to migrate to the T cell areas of lymph nodes as well as on their maturation state. Depending on their cytokine-secreting profile, DC are able to skew the immune response in a specific direction. In particular, IL-12p70 producing DC drive T cells towards a T helper 1 type response. A serious disadvantage of current clinical grade ex vivo generated monocyte-derived DC is the poor IL-12p70 production. We have investigated the effects of Toll-like receptor (TLR)-mediated maturation on ex vivo generated human monocyte-derived DC. We demonstrate that in contrast to cytokine-matured DC, DC matured with poly(I:C) (TLR3 ligand) and/or R848 (TLR7/8 ligand) are able to produce vast amounts of IL-12p70, but exhibit a reduced migratory capacity. The addition of prostaglandin E2 (PGE2) improved the migratory capacity of TLR-ligand matured DC while maintaining their IL-12p70 production upon T cell encounter. We propose a novel clinical grade maturation protocol in which TLR ligands poly(I:C) and R848 are combined with PGE2 to generate DC with both high migratory capacity and IL-12p70 production upon T cell encounter

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Desain Meja Kerja Pembakaran Arang Bongkarpasang Untuk Festival Kuliner Di Area Terbuka

Festival kuliner yang diadakan di luar ruangan merupakan salah satu acara publik yang jumlah peminatnya cukup tinggi. Mulai banyak penyelenggara dan pengusaha kuliner muncul untuk mengikuti acara tersebut. Jenis kuliner yang dijual bermacam-macam dari makanan luar negeri hingga makanan tradisional khas Indonesia. Beberapa jenis makanan tradisional khas Indonesia masih dimasak dengan menggunakan arang sebagai bahan bakarnya. Jika tidak menggunakan arang, citarasa dan aroma khas makanan tersebut hilang. Karena itu dibutuhkan dapur berbahan bakar arang yang dapat memenuhi kebutuhan tersebut dengan tingkat efisiensi terhadap instalasi dapur, sistem kerja, layout dapur, dan sirkulasi udara yang baik sehingga dapat digunakan di festival kuliner

DINAMIKA KESUKSESAN KARIR JOB-HOPPER PADA BOUNDARYLESS CAREER (Studi Fenomenologi)

This phenomenology qualitative research was conducted in order to know the dynamics of career success among job hopper and how did job hoppers interpret the meaning career success in boundaryless career. This research used in-depth interview for collecting the data and supported by the data from documents. The respondents involved in this research were four job hoppers in the level of manager, five significant others, such as family, workmate, and steady career. The findings revealed that: the job-hoppers individuals who frequently movement of job from one place to another place because of intrinsic factors (financial, interpersonal conflict, alternative employment opportunities, etc), extrinsic factors (challenges, willing to learn, personal characteristics, family, etc), proponent factors (networking, family support, and media). The meanings of career success among job-hopper in the boundaryless career wer

Pelaksanaan Program Remedial pada Mata Pelajaran Sosiologi Kelas X di SMA Negeri 4 Pontianak

This thesis entitled "Implementation of Remedial Lesson In Sociology at SMAN 4 Pontianak X class. This research uses the case study method. Sources of data in this study is the XE students classroom at SMAN 4 Pontianak. Data collection techniques used were observation, interviews, and documentary study, while tools of data collection, using your checklists, interviews, and documentary study. The data analysis technique used is redukasi, data presentation, and conclusion / verification, while for testing the validity of the data using triangulation techniques, reference material, and a member check. From the processing of the data obtained in the study of the test scores of students, both first or after taking remedial showed an increase. all of students who take the first exam in class XE are 36 students. Students who achieve mastery on the first exam are 23 students, while students who do not achieve mastery are 13 students, but after being given remedial all students achieve mastery. So In the implementation of remedial programs are showed an increase in student learning outcomes