Electrode processes during the electrorefiniment of lead in the KCl-PbCl2-PbO melt

The influence of PbO addition on current efficiency during the electrorefinement of lead in the KCl-PbCl2-PbO melt was investigated. It was shown that with PbO concentration in the KCl-PbCl2 eqiumolar mixture increasing, the current efficiency of lead decreases. Electrode processes mechanism is proposed

A novel hypervariable variable number tandem repeat in the dopamine transporter gene (SLC6A3)

The dopamine transporter gene, SLC6A3, has received substantial attention in genetic association studies of various phenotypes. Although some variable number tandem repeats (VNTRs) present in SLC6A3 have been tested in genetic association studies, results have not been consistent. VNTRs in SLC6A3 that have not been examined genetically were characterized. The Tandem Repeat Annotation Library was used to characterize the VNTRs of 64 unrelated long-read haplotype-phased SLC6A3 sequences. Sequence similarity of each repeat unit of the five VNTRs is reported, along with the correlations of SNP-SNP, SNP-VNTR, and VNTR-VNTR alleles across the gene. One of these VNTRs is a novel hyper-VNTR (hyVNTR) in intron 8 of SLC6A3, which contains a range of 3.4-133.4 repeat copies and has a consensus sequence length of 38 bp, with 82% G+C content. The 38-base repeat was predicted to form G-quadruplexes in silico and was confirmed by circular dichroism spectroscopy. In addition, this hyVNTR contains multiple putative binding sites for PRDM9, which, in combination with low levels of linkage disequilibrium around the hyVNTR, suggests it might be a recombination hotspot

Intestinal Microbiota Composition of Interleukin-10 Deficient C57BL/6J Mice and Susceptibility to Helicobacter hepaticus-Induced Colitis

The mouse pathobiont Helicobacter hepaticus can induce typhlocolitis in interleukin-10-deficient mice, and H. hepaticus infection of immunodeficient mice is widely used as a model to study the role of pathogens and commensal bacteria in the pathogenesis of inflammatory bowel disease. C57BL/6J Il10[superscript −/−] mice kept under specific pathogen-free conditions in two different facilities (MHH and MIT), displayed strong differences with respect to their susceptibilities to H. hepaticus-induced intestinal pathology. Mice at MIT developed robust typhlocolitis after infection with H. hepaticus, while mice at MHH developed no significant pathology after infection with the same H. hepaticus strain. We hypothesized that the intestinal microbiota might be responsible for these differences and therefore performed high resolution analysis of the intestinal microbiota composition in uninfected mice from the two facilities by deep sequencing of partial 16S rRNA amplicons. The microbiota composition differed markedly between mice from both facilities. Significant differences were also detected between two groups of MHH mice born in different years. Of the 119 operational taxonomic units (OTUs) that occurred in at least half the cecum or colon samples of at least one mouse group, 24 were only found in MIT mice, and another 13 OTUs could only be found in MHH samples. While most of the MHH-specific OTUs could only be identified to class or family level, the MIT-specific set contained OTUs identified to genus or species level, including the opportunistic pathogen, Bilophila wadsworthia. The susceptibility to H. hepaticus-induced colitis differed considerably between Il10[superscript −/−] mice originating from the two institutions. This was associated with significant differences in microbiota composition, highlighting the importance of characterizing the intestinal microbiome when studying murine models of IBD.National Institutes of Health (U.S.) (Grant NIH P01-CA26731)National Institutes of Health (U.S.) (Grant NIH P30ES0026731)National Institutes of Health (U.S.) (Grant NIH R01-OD011141

Integrated Live Help in the Information Commons

The University of Kansas implemented an "Ask a Librarian" email reference service in February 2002. With the arrival of our new dean, we were given a mandate to expand the existing email reference service to include a "live help" component. In reviewing different software packages, we were asked to consider other services that were being used or could be used locally (on campus), regionally (in the State of Kansas) or nationally where collaboration would be possible. This gave us the opportunity to move beyond traditional library information services to look for potential applications. The timing was certainly appropriate for the implementation of a live reference service at the University of Kansas Libraries for a number of reasons. The Kansas State Library was about to start a state-wide collaborative chat service (KANAnswer) in January 2003, responsibility for the University of Kansas KUINFO (a student services information telephone line) was transferred to the library administration, the main library service areas were being reconfigured into an information commons area and the Vice-Provost for Information Services had completed a strategic planning document supporting a vision of providing an integrated and cooperative service for the university community’s information needs. The convergence of all these factors led us to select a product that would have the capability to accommodate a variety of the university’s information needs

Project Management and Institutional Collaboration in Libraries

As most libraries in the United States struggle with declining financial support, combined with the ever-increasing need to incorporate new technology into services and the profusion of resources that are available, inter-institutional cooperation is becoming more common. Planning and implementing new projects in libraries is better carried out if the project is formally managed from the beginning to ensure an orderly and efficient completion to the project. Two institutions have developed new services that they sought to expand to other institutions. Though neither project set out to use project-management techniques, the development of the new services illustrates their practical use

Broadening Our Reach: Collaborating for Improvement

ACRL 12th. National ConferenceThis document contains the proposal submitted to the Association of College and Research Libraries for a poster session at the ACRL 12th. National Conference.Association of College and Research Librarie