Rapid sequence-independent cellular response to oligodeoxynucleotides

AbstractThe presence of receptors for oligodeoxynucleotides (OdN) on the surface of L929 cells has previously been described. To study the possible coupling of the receptor to cellular signal transducing systems, the effect of phosphodiester OdN of different sequences on cellular phospholipase C and protein kinase C (PKC) activities in L929 fibroblasts was studied. Treatment of cells with OdN induced an increase in 32P labeling of phosphatidic acid which was accompanied by a gradual decrease in diacylglycerol. These effects seem to be independent of the OdN sequence. PKC activity in membranes isolated from OdN-treated cells was found to be lower than that in membranes of control cells. SDS-PAGE of the 32P-labeled cellular proteins revealed that OdN treatment caused a decrease in phosphorylation of the 26 and 73 kDa cellular proteins in the cells

Диагностические и прогностические лабораторные критерии развития сепсиса при гнойно-воспалительных заболеваниях мягких тканей

Objective. Identification of laboratory parameters that are used in routine practice and can serve as diagnostic and prognostic criteria for the development of sepsis and its outcomes in patients with purulent-inflammatory diseases of soft tissues.Materials and methods. The study included 48 patients with purulent-inflammatory diseases of soft tissues. Recorded the occurrence of such clinical events as the development of sepsis or septic shock, intensive therapy, death or recovery and discharge from the hospital. For the diagnosis of sepsis, a SOFA (Sepsis-related organ failure assessment score) ³ 2 points was used. Patients were divided into subgroups according to the number of points according to the SOFA scale, intensive care and depending on the outcome of the disease: Subgroup 1 – 26 patients with sepsis (SOFA ³ 2 points) and 22 patients with systemic inflammatory response syndrome (SIRS) and SOFA <2 points; 2nd subgroup – 12 people who underwent intensive therapy and 36 people without it; 3rd subgroup – 7 patients with a fatal outcome and 41 patients with a favorable outcome.Results. In patients with sepsis, albumin concentration was 24,07 g / l in median versus 34,65 g / l in the control group of patients with SOFA <2 points (p <0,01); glucose -7,82 mmol / l and 5,15 mmol / l (p <0,01); sodium concentration of 133 mmol / li 139 mmol / l (p <0,01). The values of the international normalized ratio (INR) amounted to a median of 1,29 and 1,04 (p <0,01); activated partial thromboplastin time -36,20 seconds and 31,50 seconds (p <0,01). In the subgroup of patients for whom intensive therapy was required, the concentration of albumin was 22,34 g / l by median versus 30,10 g / l (p <0,01); urea – 15,50 mmol / l versus 6,00 mmol / l (p <0,05), glucose – 9,61 mmol / l against 5,80 mmol / l (p <0,05), lactate dehydrogenase-644,00 U / l and 426,00 U / l (p <0,05); INR – 1,35 against 1,05 (p <0,05). The aver-age total protein concentration is 47,80 g / l versus 57,90 g / l (p <0,01). The average albumin is 22,34 g / l versus 28,50 g / l (p <0,05). The glucose concentration among patients with a fatal outcome was 12,00 mmol / l in median versus 5,95 mmol / l (p <0,01); urea – 23,22 mmol / l versus 6,00 mmol / l (p <0,01). The incidence of lethal disease was statistically significantly higher in patients with a total protein level of less than 52 g / l 5,96 times (RR = 5,96, 95% CI 1,32 – 26,89), glucose more than 11 mmol / l – 7,00 times (OR = 7,00, 95% CI 1,25 – 39,15), urea more than 20 mmol / l – 7,05 times (RR = 7,05, 95% CI 2,00 – 24,85).Conclusion. Routine laboratory indicators as the level of total protein, albumin, glucose, sodium and urea, as well as indicators of the blood coagulation system (INR and APTT), can serve as diagnostic and prognostic criteria for the development of sepsis and its outcomes in patients with purulentinflammatory diseases. soft tissue.Цель: выявление лабораторных показателей, которые используются в рутинной практике и могут служить диагностическими и прогностическими критериями развития сепсиса и его исходов у пациентов с гнойно-воспалительными заболеваниями мягких тканей.Материалы и методы. В исследование включено 48 пациентов с гнойно-воспалительными заболеваниями мягких тканей. Проводили учет наступления таких клинических событий, как развитие сепсиса или септического шока, проведение интенсивной терапии, летальный исход или выздоровление и выписка из стационара. Для постановки диагноза «сепсис» применяли шкалу SOFA (Sepsis-related organ failure assessment score) ³ 2 баллов. Пациенты были распределены в подгруппы по количеству баллов по шкале SOFA, проведения интенсивной терапии и в зависимости от исхода заболевания: 1-я подгруппа – 26 больных сепсисом (SOFA ³ 2 баллов) и 22 пациента с синдромом системного воспалительного ответа (ССВР) и SOFA < 2 баллов; 2-я подгруппа – 12 человек, которым проводилась интенсивная терапия, и 36 человек без нее; 3-я подгруппа – 7 больных с летальным исходом и 41 пациент с благоприятным исходом.Результаты. У пациентов с сепсисом концентрация альбумина составила по медиане 24,07 г/л против 34,65 г/л в контрольной группе больных с SOFA < 2 баллов (p< 0,01); глюкозы – 7,82 ммоль/л и 5,15 ммоль/л (p< 0,01); концентрация натрия 133 ммоль/л и 139 ммоль/л (p< 0,01). Значения международного нормализованного отношения (МНО) составили по медиане 1,29 и1,04 (p< 0,01); активированного частичного тромбопластинового времени – 36,20 с и 31,50 с (p< 0,01). В подгруппе пациентов, которым потребовалось проведение интенсивной терапии, концентрации альбумина составили по медиане 22,34 г/л против 30,10 г/л (p< 0,01); мочевины – 15,50 ммоль/л против 6,00 ммоль/л (p< 0,05), глюкозы– 9,61 ммоль/лпротив 5,80 ммоль/л(p< 0,05), лактатдегидрогеназы – 644,00 Ед/л и 426,00 Ед/л (p< 0,05); МНО – 1,35 против 1,05 (p< 0,05). Средняя концентрация общего белка – 47,80 г/л против 57,90 г/л (p < 0,01). Средние показатели альбумина – 22,34 г/л против 28,50 г/л (p < 0,05). Концентрация глюкозы среди пациентов с летальным исходом составила по медиане 12,00 ммоль/л против 5,95 ммоль/л (p< 0,01); мочевины – 23,22 ммоль/л против 6,00 ммоль/л (p < 0,01). Частота наступления летального исхода болезни была статистически значимо выше у пациентов с уровнем общего белка менее 52 г/л в 5,96 раз (ОР = 5,96, 95 % ДИ 1,32 – 26,89), глюкозы более 11 ммоль/л – в 7,00 раз (ОР = 7,00, 95 % ДИ 1,25 – 39,15), мочевины более 20 ммоль/л – в 7,05 раз (ОР = 7,05, 95 % ДИ 2,00 – 24,85).Заключение. Такие рутинные лабораторные показатели, как уровень общего белка, альбумина, глюкозы, натрия и мочевины, а также показатели свертывающей системы крови (МНО и АЧТВ) могут служить диагностическими и прогностическими критериями развития сепсиса и его исходов у пациентов с гнойно-воспалительными заболеваниями мягких тканей

‘In vivo’ optical approaches to angiogenesis imaging

In recent years, molecular imaging gained significant importance in biomedical research. Optical imaging developed into a modality which enables the visualization and quantification of all kinds of cellular processes and cancerous cell growth in small animals. Novel gene reporter mice and cell lines and the development of targeted and cleavable fluorescent “smart” probes form a powerful imaging toolbox. The development of systems collecting tomographic bioluminescence and fluorescence data enabled even more spatial accuracy and more quantitative measurements. Here we describe various bioluminescent and fluorescent gene reporter models and probes that can be used to specifically image and quantify neovascularization or the angiogenic process itself

Immunochromatographic rapid test to determine β-hemolytic streptococcus group A in patients with erysipelas

Introduction. Traditionally, erysipelas has been associated with streptococcal infection. Taking into account the possibility of carriage of the pathogen on the mucous membranes of the upper respiratory tract, we assumed that in patients with erysipelas of various localization, β-hemolytic streptococcus of group A would be detected. Objective: Determine the effectiveness of immunochromatographic test for detecting β-hemolytic streptococcus group A in patients with erysipelas. Materials and methods: The study included 52 patients with various forms of erysipelas. The diagnosis of «Erysipelas» was established clinically. Patients were examined using routine clinical and laboratory methods. To identify the β-hemolytic streptococcus group A antigen, a smear was taken from the mucous membrane of the back wall of the pharynx and tonsils in all patients at the hospital, and a sandwich-membrane immunochromatographic test was used. In order to identify the pathogen from the source of inflammation, in the case of complicated forms of erysipelas, bacteriological method was used. Results: Immunochromatographic smear test revealed the presence of β-hemolytic streptococcus group A in 3 out of 52 patients (7%). Bacteriological examination of the contents of the wound, conducted by 16 patients (31%), did not reveal β-hemolytic streptococcus group A in any of the patients. One of these patients (with a fatal outcome) had a positive result of an immunochromatographic test (2%). In the wound discharge, 8 patients detected bacteria of the family Staphylococcaceae, including in combination with Enterococcus faecalis, Klebsiella mobilis, Proteus Mirabilis and Pseudomonas aeruginosa. In one case, Acinetobacter baumanii from the Moraxellaceae family was isolated. Conclusion: We have not established the effectiveness of the immunochromatographic test for determining β-hemolytic streptococcus group A in the emergency department of a surgical hospital in patients with erysipelas. This is probably due to the frequent use of antibacterial drugs in the prehospital phase. It is impossible to exclude the leading role of other etiological factors in the development of inflammation of soft tissues, clinically similar to streptococcal infection

Equivalence of the method of the kinetic equation and the fluctuating-frequency method in the theory of the broadening of spectral lines

International audienceA new expression for the Stark profiles of spectral lines in plasma has been obtained by the method of the kinetic equation taking into account the dynamics of the plasma microfield. The result represents a dynamic line profile in the form of simple functionals of a static profile. The relation of the new solution with the known fluctuatingﰀfrequency method has been analyzed. It has been shown that this method is a discrete analog of the method of the kinetic equation and passes to the latter method in the limit of the continuous fluctuations. Simple formulas (4), (5), and (21) for dynamic line profiles provide ultrafast calculations of the profiles of spectral lines taking into account the dynamics of the plasma microfield

Diagnostic and prognostic laboratory criteria for the development of sepsis in purulent-inflammatory diseases of soft tissues

Objective. Identification of laboratory parameters that are used in routine practice and can serve as diagnostic and prognostic criteria for the development of sepsis and its outcomes in patients with purulent-inflammatory diseases of soft tissues.Materials and methods. The study included 48 patients with purulent-inflammatory diseases of soft tissues. Recorded the occurrence of such clinical events as the development of sepsis or septic shock, intensive therapy, death or recovery and discharge from the hospital. For the diagnosis of sepsis, a SOFA (Sepsis-related organ failure assessment score) ³ 2 points was used. Patients were divided into subgroups according to the number of points according to the SOFA scale, intensive care and depending on the outcome of the disease: Subgroup 1 – 26 patients with sepsis (SOFA ³ 2 points) and 22 patients with systemic inflammatory response syndrome (SIRS) and SOFA <2 points; 2nd subgroup – 12 people who underwent intensive therapy and 36 people without it; 3rd subgroup – 7 patients with a fatal outcome and 41 patients with a favorable outcome.Results. In patients with sepsis, albumin concentration was 24,07 g / l in median versus 34,65 g / l in the control group of patients with SOFA <2 points (p <0,01); glucose -7,82 mmol / l and 5,15 mmol / l (p <0,01); sodium concentration of 133 mmol / li 139 mmol / l (p <0,01). The values of the international normalized ratio (INR) amounted to a median of 1,29 and 1,04 (p <0,01); activated partial thromboplastin time -36,20 seconds and 31,50 seconds (p <0,01). In the subgroup of patients for whom intensive therapy was required, the concentration of albumin was 22,34 g / l by median versus 30,10 g / l (p <0,01); urea – 15,50 mmol / l versus 6,00 mmol / l (p <0,05), glucose – 9,61 mmol / l against 5,80 mmol / l (p <0,05), lactate dehydrogenase-644,00 U / l and 426,00 U / l (p <0,05); INR – 1,35 against 1,05 (p <0,05). The aver-age total protein concentration is 47,80 g / l versus 57,90 g / l (p <0,01). The average albumin is 22,34 g / l versus 28,50 g / l (p <0,05). The glucose concentration among patients with a fatal outcome was 12,00 mmol / l in median versus 5,95 mmol / l (p <0,01); urea – 23,22 mmol / l versus 6,00 mmol / l (p <0,01). The incidence of lethal disease was statistically significantly higher in patients with a total protein level of less than 52 g / l 5,96 times (RR = 5,96, 95% CI 1,32 – 26,89), glucose more than 11 mmol / l – 7,00 times (OR = 7,00, 95% CI 1,25 – 39,15), urea more than 20 mmol / l – 7,05 times (RR = 7,05, 95% CI 2,00 – 24,85).Conclusion. Routine laboratory indicators as the level of total protein, albumin, glucose, sodium and urea, as well as indicators of the blood coagulation system (INR and APTT), can serve as diagnostic and prognostic criteria for the development of sepsis and its outcomes in patients with purulentinflammatory diseases. soft tissue