SLAM Project - Long Term Ecological Study of the Impacts of Climate Change in the natural forest of Azores: IV - The spiders of Terceira and Pico Islands (2019-2021) and general diversity patterns after ten years of sampling

BACKGROUND: Long-term studies are key to understand the drivers of biodiversity erosion, such as land-use change and habitat degradation, climate change, invasive species or pollution. The long-term project SLAM (Long Term Ecological Study of the Impacts of Climate Change in the natural forest of Azores) started in 2012 and focuses on arthropod monitoring, using SLAM (Sea, Land and Air Malaise) traps, aiming to understand the impact of the drivers of biodiversity erosion on Azorean native forests (Azores, Portugal). This is the fourth contribution including SLAM project data and the second focused on the spider fauna (Arachnida, Araneae) of native forests on two islands (Pico and Terceira). In this contribution, we describe data collected between 2019 and 2021 and we analyse them together with a previously published database that covered the 2012-2019 period, in order to describe changes in species abundance patterns over the last ten years. NEW INFORMATION: We present abundance data of Azorean spider species for the 2019-2021 period in two Azorean Islands (Terceira and Pico). We also present analyses of species distribution and abundance of the whole sampling period. In the period of 2019-2021, we collected a total of 5110 spider specimens, of which 2449 (48%) were adults. Most juveniles, with the exception of some exotic Erigoninae, were also included in the data presented in this paper, since the low diversity of spiders in the Azores allows a relatively precise species-level identification of this life-stage. We recorded a total of 45 species, belonging to 39 genera and 16 families. The ten most abundant species were composed mostly of endemic or native non-endemic species and only two exotic species (Tenuiphantes tenuis (Blackwall, 1852) and Dysdera crocata C. L. Koch, 1838). They included 4308 individuals (84%) of all sampled specimens and were the dominant species in Azorean native forests. The family Linyphiidae was the richest and most abundant taxon, with 15 (33%) species and 2630 (51%) specimens. We report Cheiracanthium mildei L. Koch, 1864, a non-native species, from Pico Island for the first time. We found no new species records on Terceira Island. This publication contributes to increasing the baseline information for future long-term comparisons of the spiders on the studied sites and the knowledge of the arachnofauna of the native forests of Terceira and Pico, in terms of species abundance, distribution and diversity across seasons for a 10 years period.AMCS is supported by the Ramón y Cajal program (RYC2020-029407-I), financed by the Spanish Ministerio de Ciencia e Innovación. IRA and MB were supported by FCT - DL57/2016/CP1375/CT0004 and /CT0001, respectively. PAVB and RG performed reserach within the project -Portal da Biodiversidade dos Açores (2022-2023) - PO Azores Project - M1.1.A/INFRAEST CIENT/001/2022. Data curation and Open Access of this manuscript was supported by the project MACRISK-Trait-based prediction of extinction risk and invasiveness for Northern Macaronesian arthropods (FCT-PTDC/BIA-CBI/0625/2021).info:eu-repo/semantics/publishedVersio

Updated Checklist of Arthropods from Azores (Portugal)

The data we present consists in an updated checklist of the Azorean arthropods. The checklist compile known records based on published literature, unpublished data, and new records at archipelago and island levels. This publication represents the most recent information about distribution of introduced, native and endemic arthropods in the Azores archipelago. Currently the total number of terrestrial arthropod taxa (species and subspecies) in the Azores is estimated of about 2417 belonging to 14 classes, 53 orders, 438 families, 1554 genera and 2397 species and 149 individual subspecies. The most diverse orders of Azorean arthropods are: Coleoptera (585 taxa), Diptera (422 taxa), Hemiptera (338 taxa), Hymenoptera (162 taxa), Lepidoptera (159 taxa) and Araneae (132 taxa). A total of 275 endemic taxa are currently known (231 species and 44 subspecies), belonging to 8 classes and 22 orders. São Miguel, Terceira and Pico are the islands with higher number of endemic species and subspecies. Compared to the other nearest Macaronesian archipelagos (Madeira and Canaries), the Azorean arthropod fauna is characterized by a lower percentage of endemism and a high proportion of exotic introduced species.FUNDING: This work was financed by two main projects: FEDER - AZORESBIOPORTAL –PORBIOTA (ACORES-01-0145-FEDER-000072) and Portal da Biodiversidade dos Açores (2022-2023) - PO Azores Project - M1.1.A/INFRAEST CIENT/001/2022. Open access was funded by the project FCT-UIDB/00329/2020-2024 (Thematic Line 1 – integrated ecological assessment of environmental change on biodiversity).info:eu-repo/semantics/publishedVersio

SLAM Project - Long Term Ecological Study of the Impacts of Climate Change in the natural forests of Azores: V - New records of terrestrial arthropods after ten years of SLAM sampling

BACKGROUND: A long-term study monitoring arthropods (Arthropoda) is being conducted since 2012 in the forests of Azorean Islands. Named "SLAM - Long Term Ecological Study of the Impacts of Climate Change in the natural forest of Azores", this project aims to understand the impact of biodiversity erosion drivers in the distribution, abundance and diversity of Azorean arthropods. The current dataset represents arthropods that have been recorded using a total of 42 passive SLAM traps (Sea, Land and Air Malaise) deployed in native, mixed and exotic forest fragments in seven Azorean Islands (Flores, Faial, Pico, Graciosa, Terceira, São Miguel and Santa Maria). This manuscript is the fifth data-paper contribution, based on data from this long-term monitoring project. NEW INFORMATION: We targeted taxa for species identification belonging to Arachnida (excluding Acari), Chilopoda, Diplopoda, Hexapoda (excluding Collembola, Lepidoptera, Diptera and Hymenoptera (but including only Formicidae)). Specimens were sampled over seven Azorean Islands during the 2012-2021 period. Spiders (Araneae) data from Pico and Terceira Islands are not included since they have been already published elsewhere (Costa and Borges 2021, Lhoumeau et al. 2022). We collected a total of 176007 specimens, of which 168565 (95.7%) were identified to the species or subspecies level. For Araneae and some Hemiptera species, juveniles are also included in this paper, since the low diversity in the Azores allows a relatively precise species-level identification of this life-stage. We recorded a total of 316 named species and subspecies, belonging to 25 orders, 106 families and 260 genera. The ten most abundant species were mostly endemic or native non-endemic (one Opiliones, one Archaeognatha and seven Hemiptera) and only one exotic species, the Julida Ommatoiulus moreleti (Lucas, 1860). These ten species represent 107330 individuals (60%) of all sampled specimens and can be considered as the dominant species in the Azorean native forests for the target studied taxa. The Hemiptera were the most abundant taxa, with 90127 (50.4%) specimens. The Coleoptera were the most diverse with 30 (28.6%) families. We registered 72 new records for many of the islands (two for Flores, eight for Faial, 24 for Graciosa, 23 for Pico, eight for Terceira, three for São Miguel and four for Santa Maria). These records represent 58 species. None of them is new to the Azores Archipelago. Most of the new records are introduced species, all still with low abundance on the studied islands. This publication contributes to increasing the baseline information for future long-term comparisons of the arthropods of the studied sites and the knowledge of the arthropod fauna of the native forests of the Azores, in terms of species abundance, distribution and diversity throughout seasons and years.AMCS is supported by the Ramón y Cajal program (RYC2020-029407-I), financed by the Spanish Ministerio de Ciencia e Innovación. IRA and MB were funded by Portuguese funds through FCT – Fundação para a Ciência e a Tecnologia, I.P., under the Norma Transitória – DL 57/2016/CP1375/CT0003 and DL 57/2016/CP1375/CT0001, respectively. Several projects supported the acquisition of traps during the last ten years, namely: EUFCT-NETBIOME –ISLANDBIODIV grant 0003/2011 (between 2012 and 2015); Portuguese National Funds, through FCT – Fundação para a Ciência e Tecnologia, within the project UID/BIA/00329/2013-2020; Direcção Regional do Ambiente - PRIBES (LIFE17 IPE/PT/ 000010) (2019); Direcção Regional do Ambiente – LIFE-BETTLES (LIFE18 NAT_PT_000864) (2020); AZORESBIOPORTAL – PORBIOTA (ACORES-01-0145- FEDER-000072) (2019); (FCT) - MACRISK-Trait-based prediction of extinction risk and invasiveness for Northern Macaronesian arthropods (FCT-PTDC/BIA-CBI/0625/2021) (2021-2022). Data curation and open Access of this manuscript were supported by the project MACRISK-Trait-based prediction of extinction risk and invasiveness for Northern Macaronesian arthropods (FCT-PTDC/BIA-CBI/0625/2021).info:eu-repo/semantics/publishedVersio

PTK7: A cell polarity receptor with multiple facets

International audienc

SLAM Project - Long Term Ecological Study of the Impacts of Climate Change in the natural forest of Azores : IV - The spiders of Terceira and Pico Islands (2019-2021) and general diversity patterns after ten years of sampling

Peer reviewe

Protein tyrosine kinase 7 has a conserved role in Wnt/beta-catenin canonical signalling

International audienc

SLAM Project - Long Term Ecological Study of the Impacts of Climate Change in the natural forests of Azores : V - New records of terrestrial arthropods after ten years of SLAM sampling

Peer reviewe

Standardization of Flow Cytometric Immunophenotyping for Hematological Malignancies: The FranceFlow Group Experience.

Flow cytometry is broadly used for the identification, characterization, and monitoring of hematological malignancies. However, the use of clinical flow cytometry is restricted by its lack of reproducibility across multiple centers. Since 2006, the EuroFlow consortium has been developing a standardized procedure detailing the whole process from instrument settings to data analysis. The FranceFlow group was created in 2010 with the intention to educate participating centers in France about the standardized instrument setting protocol (SOP) developed by the EuroFlow consortium and to organise several rounds of quality controls (QCs) in order to evaluate the feasibility of its application and its results. Here, we report the 5 year experience of the FranceFlow group and the results of the seven QCs of 23 instruments, involving up to 19 centers, in France and in Belgium. The FranceFlow group demonstrates that both the distribution and applicability of the SOP have been successful. Intercenter reproducibility was evaluated using both normal and pathological blood samples. Coefficients of variation (CVs) across the centers were <7% for the percentages of cell subsets and <30% for the median fluorescence intensities (MFIs) of the markers tested. Intracenter reproducibility provided similar results with CVs of <3% for the percentages of the majority of cell subsets, and CVs of <20% for the MFI values for the majority of markers. Altogether, the FranceFlow group show that the 19 participating labs might be considered as one unique laboratory with 23 identical flow cytometers able to reproduce identical results. Therefore, SOP significantly improves reproducibility of clinical flow in hematology and opens new avenues by providing a robust companion diagnostic tool for clinical trials in hematology. © 2019 International Society for Advancement of Cytometry