Phloroglucinol is Effective for in vitro Growth and Multiplication of Musa accuminata Cv. Grand Naine Shoots and Roots

Despite being a major staple food in the world, banana production in the United States is still limited, with about 500 acres under cultivation. Micropropagation has been an effective method for the large-scale production of bananas to meet both domestic and international markets. However, the efficiency of micropropagation protocols depends on several factors, particularly on the types, combinations, and levels of plant growth regulators used in the culture media. Phloroglucinol is a growth regulator that acts synergistically with auxins and cytokinins. The use of phloroglucinol for the production and development of in vitro plantlets of Musa spp. cv. Grande Naine were investigated. Multiplication and elongation of shoots and roots in vitro was enhanced by the addition of 200 μM phloroglucinol to MS medium, as compared to the control with 13.2 μM BA. Higher concentrations (400 to 1000 μM phloroglucinol) resulted in reduced growth and development of shoots and roots in vitro

Cardiovascular complications related to cocaine use: case report

BACKGROUND AND OBJECTIVES: Cocaine is the most commonly used illicit drug and its acute and chronic effects are related to a variety of physiological changes, mainly in the cardiovascular system. This study is a case report of a patient with cardiomyopathy related to cocaine use. CASE REPORT: A 19 year old men, who has been using cocaine and crack since 15 years old, was admitted to the emergency department (ED) in February 2006 with progressive dyspnea during minimal efforts and bloody expectoration. During the physical exam it was observed legs edema, jugular stasis and dyspnea at rest. The echocardiogram demonstrated left ventricular hypocinesia, a 17 mm ventricular thrombus and a 12% ejection fraction. A bleeding from the left upper lobe was identified during a pulmonary bronchoscopy which was treated with arterial embolization. After 48h of the procedure, the patient was asymptomatic and an antithrombotic treatment with warfarin and enoxaparin was started. No obstruction was found at the cineangiography and the patient was discharged after clinical improvement. The patient was admitted again to the intensive care unit in July with intense chest pain and dyspnea at rest. A new cineangiography was performed and it was observed occlusion in the anterior descendent coronary artery. CONCLUSIONS: The cocaine acute effects are commonly seen at the ED but the chronic effects, as the cardiovascular manifestations, can take longer to be correlated as a side effect of cocaine use. Its prolonged use is related to left ventricular systolic dysfunction due to hypertrophy or myocardial dilation, atherosclerosis, arrhythmias, myocyte apoptosis and sympathetic damage.JUSTIFICATIVA E OBJETIVOS: A cocaína é uma droga ilícita amplamente utilizada e o seu uso tem sido associado a efeitos decorrentes da toxicidade aguda e crônica em praticamente todos os órgãos, particularmente no sistema cardiovascular. Este artigo visou descrever um caso de cardiomiopatia em paciente jovem usuário crônico de cocaína. RELATO DO CASO: Paciente do sexo masculino, 19 anos, usuário de cocaína por inalação e crack desde os 15 anos de idade. Foi internado em fevereiro de 2006 devido a dispnéia progressiva aos mínimos esforços e expectoração sanguinolenta. Ao exame físico apresentava edema nos membros inferiores, estase jugular e dispnéia em repouso. Foram observados no ecocardiograma: dilatação das quatro câmaras cardíacas, com hipocinesia difusa de ventrículo esquerdo (VE), trombo mural em VE de 17 mm e fração de ejeção de 12%. Realizada broncoscopia pulmonar que identificou sangramento em língula ativo, tratado com embolização. Após 48h do procedimento, o paciente manteve-se assintomático e sem expectoração sanguinolenta. Iniciado tratamento antitrombótico com warfarina e enoxaparina. A cineangiocoronariografia não evidenciou lesões obstrutivas e o paciente recebeu alta após melhora clínica. Re-internado em julho de 2006 com dor precordial de forte intensidade e dispnéia de repouso. Nova cineangiocoronariografia evidenciou oclusão de terço médio da artéria descendente anterior. CONCLUSÕES: Os efeitos agudos da cocaína freqüentemente motivam atendimento de emergência. Já as suas manifestações crônicas, como as doenças cardiovasculares, podem produzir alterações de difícil correlação futura ao seu consumo prévio. O uso prolongado da cocaína está relacionado à alteração da função sistólica ventricular esquerda por hipertrofia ou dilatação miocárdica, aterosclerose, disritmias cardíacas, apoptose de cardiomiócitos e lesão simpática.UNIFESPUniversidade São FranciscoUNIFESPSciEL

Root production and quality attributes of sweet potato genotypes in Brasília - DF, Brazil, during two cropping seasons.

Avaliou-se nove genótipos de batata-doce: BRS Amélia, Beauregard, Brazlândia Branca, Brazlândia Rosada, Brazlândia Roxa, BRS Cuia, Princesa, BRS Rubissol e um genótipo local em delineamento de blocos casualizados, com quatro repetições. Estimou-se características relacionadas a produção de raízes, características de qualidade e danos causador por insetos

Anaplasma marginale infection in cattle from southwestern Amazonia.

study provides the first epidemiological data regarding infection by Anaplasma marginale in cattle reared in south-western Brazilian Amazonia. One simple procedure was adapted for the extraction of DNA from blood clots collected in seven microregions of Rondônia State and two mesoregions of Acre State. PCR method was used to asses the frequency of A. marginale infections in 4 to12-month-old cattle. The cattle infection was investigated by polymerase chain reaction (PCR) using the specific primer ?msp5? for A. marginale. The DNA amplifications revealed that the mean frequency of A. marginale infection was 98.6% (1,627/1,650) in samples from Rondonia, and 92.87% (208/225) in samples from Acre. The high frequency of A. marginale infections in 4 to 12- month-old cattle indicate a situation of enzootic stability in the studied areas and are comparable to those detected by immunodiagnosis in different endemic regions in Brazil. The DNA extraction of clotted blood method described here can be used for epidemiological studies on anaplasmosis and other bovine hemoparasites

In vitro viability of pollen grain of fiji dwarf coconut accession.

Lethal yellowing is a disease that causes severe damages to coconut palms and it has devastated the coconut canopy of several Caribbean islands, and areas of southern Florida. The Fiji dwarf coconut accession has demonstrated tolerance to this disease. Pollen storage and germination provides useful information for conservation strategy and genetic improvement programs. The objective of this study was to evaluate pollen viability of Fiji dwarf coconut by in vitro germination under different nutritional and abiotic conditions. For the first experiment, two liquid culture media were evaluated for in vitro germination of Fiji dwarf coconut pollen; the Lora culture medium and the Armendariz culture medium combined with four temperature levels. In the second experiment, different sucrose concentrations in the Lora culture medium (4, 8, 12 and 16%) were evaluated combined with two incubation times (6 and 24 h) in the presence or absence of light. The highest in vitro germination of pollen grain was obtained in Lora culture medium by 24 h incubation period at 28 ºC. The light condition does not influence the germination process. In vitro germination percentage improved as the sucrose concentration increased according to a linear regression. In contrast, damage pollen grain percentage reduced according to a quadratic regression

Quebra de dormência em sementes botânicas de batata-doce utilizando diferentes compostos químicos.

O presente trabalho teve por objetivo avaliar o efeito de diferentes compostos químicos e tempos de contato para quebra de dormência de sementes botânicas de batata-doce.bitstream/item/217015/1/BPD-210-23-out-2020.pd

Diversidade genética em germoplasma internacional de pinhão-manso.

O pinhão-manso (Jatropha curcas L.) é uma oleaginosa em potencial, que tem despertado ointeresse de produtores, governos e instituições de pesquisa, visando aodesenvolvimento de tecnologia que possibilite sua utilização como cultura agroenergética

Produtividade de batata-doce em canteiros utilizando diferentes espaçamentos e segmentos da rama.

O objetivo deste estudo foi avaliar o desempenho produtivo de batatas-doces em canteiros utilizando espaçamentos distintos e mudas oriundas de diferentes segmentos da rama.bitstream/item/212362/1/BPD-199.pd