Application of the cDNA-AFLP method for studying gene expression in Fibrobacter succinogenes S85 exposed to 134.2 kHz electromagnetic field

AbstractMany biological effects related to the exposure of cells and tissues to electromagnetic fields have been reported in the literature, including those influencing DNAs and RNAs structure and ..

Farm efficiency related to animal welfare performance and management of sheep farms in marginal areas of Central Italy: a two-stage DEA model

The development of specific actions to increase animal health and welfare is indicated as a strategy to improve the efficiency and sustainability of many livestock systems, including sheep farming. In this paper, efficiency measures are provided to confirm the hypothesis that farms that are higher-performing in terms of animal welfare and management are also more technically efficient. A two-stage Data Envelopment Analysis (DEA) approach was adopted with the following twofold objectives: 1) to evaluate the efficiency and super-efficiency of 76 meat-producing sheep farms situated in marginal lands in central Italy, through DEA and Super-DEA (S-DEA) models; and 2) to assess the influence of animal welfare and management indicators on technical efficiency values through the application of a Tobit regression model. An overall efficiency performance varying within a range of 0.44–1 was estimated, with an average value of 0.80, implying a potential increase of 20% in terms of output production from both management and scale improvements. The ‘pure’ technical inefficiency was found to contribute three times more than scale inefficiency in determining the overall technical inefficiency. Adopting a more extensive farming system and increasing replacement rate were found to affect negatively the efficiency scores. On the other hand, having less than 5% of animals with body condition score beyond acceptable limits, presence of access control structures, well managed lambing pens, and dedicated feed stocking areas resulted in a positive influence on efficiency. Improvements in animal welfare aspects did not appear to be farm-scale-dependent.Highlights DEA and Super-DEA models were applied to assess sheep farm technical efficiency in Central Italy. An overall potential 20% increase in output production was estimated. Animal welfare factors were found to significantly affect efficiency performance

Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 positively affects performance and intestinal ecosystem in broilers during a campylobacter jejuni infection

In poultry production, probiotics have shown promise to limit campylobacteriosis at the farm level, the most commonly reported zoonosis in Europe. The aim of this trial was to evaluate the effects of Saccharomyces supplementation in Campylobacter jejuni challenged chickens on performance and intestinal ecosystem. A total of 156 day old male Ross 308 chicks were assigned to a basal control diet (C) or to a Saccharomyces cerevisiae boulardii CNCM I-1079 supplemented diet (S). All the birds were orally challenged with C. jejuni on day (d) 21. Live weight and growth performance were evaluated on days 1, 21, 28 and 40. The histology of intestinal mucosa was analyzed and the gut microbiota composition was assessed by 16S rRNA. Performance throughout the trial as well as villi length and crypt depth were positively influenced by yeast supplementation. A higher abundance of operational taxonomic units (OTUs) annotated as Lactobacillus reuteri and Faecalibacterium prausnitzii and a lower abundance of Campylobacter in fecal samples from S compared to the C group were reported. Supplementation with Saccharomyces cerevisiae boulardii can effectively modulate the intestinal ecosystem, leading to a higher abundance of beneficial microorganisms and modifying the intestinal mucosa architecture, with a subsequent improvement of the broilers' growth performance

Effects of Goji berries supplementation on the productive performance of rabbit

Abstract Recognized by the traditional medicine and recent scientific research studies, Lycium barbarum berries (Goji berries) have beneficial effects on human and animal health. The aim of our study was to evaluate the effects of Goji berries on the productive performance of rabbits. One month before insemination, 60 New Zealand White does were randomly assigned to one of the following 3 dietary treatments: commercial standard diet (C); C supplemented with 1% Goji berries (LG); and C supplemented with 3% Goji berries (HG). After weaning up to 91 days of age, 15 randomly selected rabbits/group were fed the same diet as the mothers (C, LG, and HG). Non-pregnant and lactating does of C group showed the highest feed intake (P < 0.01), although no significant differences in body weight (BW) were observed between groups. Nutritional treatment did not affect the offspring's feed intake. However, the rabbits fed with Goji supplementation showed not only higher mean BW both during growth (P < 0.001) and at slaughter (P < 0.01), but also better feed conversion ratio (FCR; P < 0.01) than the control group. Rabbits of LG group showed the best performances in the pre-weaning period which was probably related to the highest milk production of the LG does (P < 0.001). Indeed, LG group showed lower pre-weaning mortality (P < 0.05), higher litter size (P < 0.05), and higher litter weight (P < 0.05) at day 18 as well as higher litter size at weaning (P = 0.05) compared to C group. In conclusion, the present study demonstrates that a low percentage of dietary supplementation with Goji berry improves both reproductive and productive traits of rabbits. Although further studies are needed, our study paves the way for the use of Goji berries in the rabbit nutrition

The Effect of Feeding Bt MON810 Maize to Pigs for 110 Days on Intestinal Microbiota

Objective: To assess the effects of feeding Bt MON810 maize to pigs for 110 days on the intestinal microbiota. Methodology/Principal Findings: Forty male pigs (,40 days old) were blocked by weight and litter ancestry and assigned to one of four treatments; 1) Isogenic maize-based diet for 110 days (Isogenic); 2) Bt maize-based diet (MON810) for 110 days (Bt); 3) Isogenic maize-based diet for 30 days followed by a Bt maize-based diet for 80 days (Isogenic/Bt); 4) Bt maizebased diet for 30 days followed by an isogenic maize-based diet for 80 days (Bt/Isogenic). Enterobacteriaceae, Lactobacillus and total anaerobes were enumerated in the feces using culture-based methods on days 0, 30, 60 and 100 of the study and in ileal and cecal digesta on day 110. No differences were found between treatments for any of these counts at any time point. The relative abundance of cecal bacteria was also determined using high-throughput 16 S rRNA gene sequencing. No differences were observed in any bacterial taxa between treatments, with the exception of the genus Holdemania which was more abundant in the cecum of pigs fed the isogenic/Bt treatment compared to pigs fed the Bt treatment (0.012 vs 0.003%; P#0.05). Conclusions/Significance: Feeding pigs a Bt maize-based diet for 110 days did not affect counts of any of the culturable bacteria enumerated in the feces, ileum or cecum. Neither did it influence the composition of the cecal microbiota, with the exception of a minor increase in the genus Holdemania. As the role of Holdemania in the intestine is still under investigatio

GMOs in animal agriculture: time to consider both costs and benefits in regulatory evaluations

In 2012, genetically engineered (GE) crops were grown by 17.3 million farmers on over 170 million hectares. Over 70% of harvested GE biomass is fed to food producing animals, making them the major consumers of GE crops for the past 15 plus years. Prior to commercialization, GE crops go through an extensive regulatory evaluation. Over one hundred regulatory submissions have shown compositional equivalence, and comparable levels of safety, between GE crops and their conventional counterparts. One component of regulatory compliance is whole GE food/feed animal feeding studies. Both regulatory studies and independent peer-reviewed studies have shown that GE crops can be safely used in animal feed, and rDNA fragments have never been detected in products (e.g. milk, meat, eggs) derived from animals that consumed GE feed. Despite the fact that the scientific weight of evidence from these hundreds of studies have not revealed unique risks associated with GE feed, some groups are calling for more animal feeding studies, including long-term rodent studies and studies in target livestock species for the approval of GE crops. It is an opportune time to review the results of such studies as have been done to date to evaluate the value of the additional information obtained. Requiring long-term and target animal feeding studies would sharply increase regulatory compliance costs and prolong the regulatory process associated with the commercialization of GE crops. Such costs may impede the development of feed crops with enhanced nutritional characteristics and durability, particularly in the local varieties in small and poor developing countries. More generally it is time for regulatory evaluations to more explicitly consider both the reasonable and unique risks and benefits associated with the use of both GE plants and animals in agricultural systems, and weigh them against those associated with existing systems, and those of regulatory inaction. This would represent a shift away from a GE evaluation process that currently focuses only on risk assessment and identifying ever diminishing marginal hazards, to a regulatory approach that more objectively evaluates and communicates the likely impact of approving a new GE plant or animal on agricultural production systems

Single-axon-resolution intravital imaging reveals a rapid onset form of Wallerian degeneration in the adult neocortex

Despite the widespread occurrence of axon degeneration in the injured and diseased nervous system, the mechanisms of the degenerative process remain incompletely understood. In particular, the factors that regulate how individual axons degenerate within their native environment in the mammalian brain are unknown. Longitudinal imaging of >120 individually injured cortical axons revealed a threshold length below which injured axons undergo a rapid-onset form of Wallerian degeneration (ROWD). ROWD consistently starts 10 times earlier and is executed 4 times slower than classic Wallerian degeneration (WD). ROWD is dependent on synaptic density, unlike WD, but is independent of axon complexity. Finally, we provide both pharmacological and genetic evidence that a Nicotinamide Adenine Dinucleotide (NAD + )-dependent pathway controls cortical axon ROWD independent of transcription in the damaged neurons. Thus, our data redefine the therapeutic window for intervention to maintain neurological function in injured cortical neurons, and support the use of in vivo optical imaging to gain unique insights into the mechanisms of axon degeneration in the brain