Impact of new synthesized analogues of dehydroacetic acid on growth rate and vomitoxin accumulation by Fusarium graminearum under different temperatures in maize hybrid

Previous work indicated that some of the new synthesized analogues of dehydroacetic acid (DHA) were inhibitory to the growth of mycotoxin producing moulds and accumulation of aflatoxin B1 (AFB1) and ochratoxin A (OTA). The objective of this study was to determine the specific new synthesized chemical compounds that may be effective against mould growth and vomitoxin (deoxynivalenol) (DON) accumulation by Fusarium graminearum. The effect of the investigated 3-/2-aminophenylamine-(ptoluoyl)- 4-hydroxy-6-(p-tolyl)-2H- pyrane-2-one (Schiff base) and 4-hydroxy-3-(p-toluoyl)-6-(p-tolil)-2Hpyrane- 2-one (DHT) on growth and DON accumulation were studied using a mould F. graminearum ZMPBF 1244 and maize grain hybrid to determine the possible use of these compounds as a mean of controlling DON accumulation. Schiff base was inhibitory at 0.05 and 0.1 μg/g and DHT at 0.5 μg/g of maize grain. The inhibitory effect of these substances was judged to be the inhibition of growth rather than toxin accumulation. When growth occurred after a delay, DON accumulation occurred when the cultures reached secondary metabolism. Given sufficient time, cultures which were inhibited initially, but which subsequently inhibited their growth, produced toxin levels equivalent to the control cultures. Levels of the Schiff base above 0.2 μg/g almost completely inhibited mould growth or permitted only a small amount of growth that never reached secondary metabolism and never produced DON during the time of this study.Key words: Fusarium graminearum, vomitoxin, Schiff base, chitin, Artemia salina

A consensus statement on detection of hippocampal sharp wave ripples and differentiation from other fast oscillations

Decades of rodent research have established the role of hippocampal sharp wave ripples (SPW-Rs) in consolidating and guiding experience. More recently, intracranial recordings in humans have suggested their role in episodic and semantic memory. Yet, common standards for recording, detection, and reporting do not exist. Here, we outline the methodological challenges involved in detecting ripple events and offer practical recommendations to improve separation from other high-frequency oscillations. We argue that shared experimental, detection, and reporting standards will provide a solid foundation for future translational discovery.This work was funded by K23NS104252 (A.A.L.) R01 MH117777 (E.B., J.W.R.) Whitehall Foundation (KH) 5F31NS120783-02 (Z.L.) 1U19NS104590 (A.L.) R01NS106611-02 (J.S., M.K.) MTEC-20-06-MOM013 (J.S., M.K.) 1U19NS107609-01 (I.S., J.L.) 1U19NS104590 (A.L., J.S.F., I.S.) 1U19NS107609 (E.A.B., J.W.R., J.J.L., I.S.) La Caixa LCF/PR/HR21/52410030 (A.N.O., L.dl.P) European Research Council Consolidator Grant 101001121 (B.P.S.) U.S.-Israel BSF grant 2017015 (RM)U01-NS113198 (J.J.) NSF CAREER IOS-1844935 (M.vdM.) 1R01NS121764-01 (B.L.M.) R01 MH122391 (G.B.) 30MH126483 (J.A.G.) Fondation pour la Recherche Médicale EQU202103012768 (M.Z.) 1R16-NS131108-01 (L.L.)

Replication of fifteen loci involved in human plasma protein N-glycosylation in 4,802 samples from four cohorts

Human protein glycosylation is a complex process, and its in vivo regulation is poorly understood. Changes in glycosylation patterns are associated with many human diseases and conditions. Understanding the biological determinants of protein glycome provides a basis for future diagnostic and therapeutic applications. Genome-wide association studies (GWAS) allow to study biology via a hypothesis-free search of loci and genetic variants associated with a trait of interest. Sixteen loci were identified by three previous GWAS of human plasma proteome N-glycosylation. However, the possibility that some of these loci are false positives needs to be eliminated by replication studies, which have been limited so far. Here, we use the largest set of samples so far (4,802 individuals) to replicate the previously identified loci. For all but one locus, the expected replication power exceeded 95%. Of the sixteen loci reported previously, fifteen were replicated in our study. For the remaining locus (near the KREMEN1 gene) the replication power was low, and hence replication results were inconclusive. The very high replication rate highlights the general robustness of the GWAS findings as well as the high standards adopted by the community that studies genetic regulation of protein glycosylation. The fifteen replicated loci present a good target for further functional studies. Among these, eight genes encode glycosyltransferases: MGAT5, B3GAT1, FUT8, FUT6, ST6GAL1, B4GALT1, ST3GAL4, and MGAT3. The remaining seven loci offer starting points for further functional follow-up investigation into molecules and mechanisms that regulate human protein N-glycosylation in vivo

GW8510 Increases Insulin Expression in Pancreatic Alpha Cells through Activation of p53 Transcriptional Activity

Background: Expression of insulin in terminally differentiated non-beta cell types in the pancreas could be important to treating type-1 diabetes. Previous findings led us to hypothesize involvement of kinase inhibition in induction of insulin expression in pancreatic alpha cells. Methodology/Principal Findings: Alpha (αTC1.6) cells and human islets were treated with GW8510 and other small-molecule inhibitors for up to 5 days. Alpha cells were assessed for gene- and protein-expression levels, cell-cycle status, promoter occupancy status by chromatin immunoprecipitation (ChIP), and p53-dependent transcriptional activity. GW8510, a putative CDK2 inhibitor, up-regulated insulin expression in mouse alpha cells and enhanced insulin secretion in dissociated human islets. Gene-expression profiling and gene-set enrichment analysis of GW8510-treated alpha cells suggested up-regulation of the p53 pathway. Accordingly, the compound increased p53 transcriptional activity and expression levels of p53 transcriptional targets. A predicted p53 response element in the promoter region of the mouse Ins2 gene was verified by chromatin immunoprecipitation (ChIP). Further, inhibition of Jun N-terminal kinase (JNK) and p38 kinase activities suppressed insulin induction by GW8510. Conclusions/Significance: The induction of Ins2 by GW8510 occurred through p53 in a JNK- and p38-dependent manner. These results implicate p53 activity in modulation of Ins2 expression levels in pancreatic alpha cells, and point to a potential approach toward using small molecules to generate insulin in an alternative cell type.Chemistry and Chemical BiologyMolecular and Cellular Biolog

Intracellular Serotonin Modulates Insulin Secretion from Pancreatic β-Cells by Protein Serotonylation

Non-neuronal, peripheral serotonin deficiency causes diabetes mellitus and identifies an intracellular role for serotonin in the regulation of insulin secretion

A consensus statement on detection of hippocampal sharp wave ripples and differentiation from other fast oscillations

Article suggests that common standards for recording, detection, and reporting for intracranial recordings in humans that suggest their role in episodic and semantic memory does not exist. Authors of the article outline the methodological challenges involved in detecting ripple events and offer practical recommendations to improve separation from other high-frequency oscillations, and argue that shared experimental, detection, and reporting standards will provide a solid foundation for future translational discovery

Visual Peoplemeter: A Vision-based Television Audience Measurement System

Visual peoplemeter is a vision-based measurement system that objectively evaluates the attentive behavior for TV audience rating, thus offering solution to some of drawbacks of current manual logging peoplemeters. In this paper, some limitations of current audience measurement system are reviewed and a novel vision-based system aiming at passive metering of viewers is prototyped. The system uses camera mounted on a television as a sensing modality and applies advanced computer vision algorithms to detect and track a person, and to recognize attentional states. Feasibility of the system is evaluated on a secondary dataset. The results show that the proposed system can analyze viewer's attentive behavior, therefore enabling passive estimates of relevant audience measurement categories

Bacterial communities associated with the production of artisanal Istrian cheese.

In this work we report on the main bacterial microflora typical for fermentation and ripening of traditional Istrian cheese. Samples from milk as well as Istrian cheese were analyzed during the ripening process by using culture independent molecular fingerprinting methods as well as culture based approaches. Our results indicate changes in bacterial diversity pattern during the ripening process. Differences in bacterial diversity at the same ripening stage among different farms investigated were comparably low. Sequence analysis of the most prominent bands of denaturing gradient gel electrophoresis fingerprints revealed dominance of Lactococcus lactis subs. lactis in all samples and a strong presence of Enterococcus spp. which was also confirmed by plate count analysis

Laminar Organization of Encoding and Memory Reactivation in the Parietal Cortex

Egocentric neural coding has been observed in parietal cortex (PC), but its topographical and laminar organization is not well characterized. We used multi-site recording to look for evidence of local clustering and laminar consistency of linear and angular velocity encoding in multi-neuronal spiking activity (MUA) and in the high-frequency (300-900 Hz) component of the local field potential (HF-LFP), believed to reflect local spiking activity. Rats were trained to run many trials on a large circular platform, either to LED-cued goal locations or as a spatial sequence from memory. Tuning to specific self-motion states was observed and exhibited distinct cortical depth-invariant coding properties. These patterns of collective local and laminar activation during behavior were reactivated in compressed form during post-experience sleep and temporally coupled to cortical delta waves and hippocampal sharp-wave ripples. Thus, PC neuron motion encoding is consistent across cortical laminae, and this consistency is maintained during memory reactivation