Thermo-mechanical process parameters effect on a 9 % Cr-2 % W steel

In this paper the effect of thermo-mechanical parameters on the mechanical behavior of a 9 % Cr-2 % W steel is investigated by hot rolling and heat treatment on pilot scale. Results show a strong effect of reheating temperature before rolling on the material hardness, due to an increase of hardenability following the austenite grain growth. A poor effect of the hot reduction and of the following tempering temperature is detected in the total investigated deformation range. A loss of impact energy is found coupled with the hardness increase. The tensile properties values are strongly depending upon the tempering temperature and an increase of tensile yield stress (Rp0,2) and ultimate stress (Rm) have been recorded in tensile test carried on at T = 550°C and T = 650°C

Nutrient availability links mitochondria, apoptosis, and obesity

Mitochondria are the dominant source of the cellular energy requirements through oxidative phosphorylation, but they are also central players in apoptosis. Nutrient availability may have been the main evolutionary driving force behind these opposite mitochondrial functions: production of energy to sustain life and release of apoptotic proteins to trigger cell death. Here, we explore the link between nutrients, mitochondria and apoptosis with known and potential implications for age‐related decline and metabolic syndromes

Cratoxylum formosum ssp. pruniflorum activates the TRAIL death receptor complex and inhibits topoisomerase I

Abstract Six species of Cratoxylum are found in Thailand. Whether the Cratoxylum formosum ssp. pruniflorum (CFP) has anticancer properties requires investigation. CFP exhibited cytotoxicity against hepatocellular carcinoma HepG2 cells. Based on FTIR microspectroscopy, CFP raised the respective lipid and nucleic acid content and β-pleated sheet in HepG2 cells, suggesting a change in the secondary protein structure. CFP induced apoptosis by increasing the activities of various caspases. Overexpression of TRAILR2 indicated the extrinsic pathway while expression of Bax/Bcl-2 ratio indicated the intrinsic pathway and decreased expression of Bid indicated cross-talk between the two. CFP alkylated the DNA and caused DNA damage, which may be the initial step in the intrinsic pathway. CFP indirectly and directly inhibited Top IB enzyme activity and decreased PARP—the protein-related DNA repair process. CFP could, thus, protect the resistance mechanism of cancer by reversing the effect of Top as confirmed by apoptosis induction in the resistant HepG2 cells. The phytochemical analysis suggested that the compounds playing an important role in the anticancer activity of CFP are a group of xanthones

An Unusual Case of Adrenal Metastasis from Colorectal Cancer: Computed Tomography and Fluorine 18-Fluoro-Deoxy-Glucose Positron Emission Tomography-Computed Tomography Features and Literature Review

Incidentally discovered adrenal masses are a common diagnostic problem. While computed tomography (CT) and magnetic resonance (MR) imaging can adequately characterize most benign or malignant adrenal masses, in some cases the results are indeterminate. We report and discuss a case of an adrenal metastasis with misleading clinical and CT features, in which an abnormal metabolic uptake detected through fluorine 18-fluoro-deoxy-glucose positron emission tomography (18F-FDG PET)-CT raised the suspicion of adrenal metastasis relatively early compared with apparently normal results on repeated follow-up CT examinations

Miglustat Reverts the Impairment of Synaptic Plasticity in a Mouse Model of NPC Disease

Niemann-Pick type C disease is an autosomal recessive storage disorder, characterized by abnormal sequestration of unesterified cholesterol within the late endolysosomal compartment of cells and accumulation of gangliosides and other sphingolipids. Progressive neurological deterioration and insurgence of symptoms like ataxia, seizure, and cognitive decline until severe dementia are pathognomonic features of the disease. Here, we studied synaptic plasticity phenomena and evaluated ERKs activation in the hippocampus of BALB/c NPC1-/- mice, a well described animal model of the disease. Our results demonstrated an impairment of both induction and maintenance of long term synaptic potentiation in NPC1-/- mouse slices, associated with the lack of ERKs phosphorylation. We then investigated the effects of Miglustat, a recent approved drug for the treatment of NPCD. We found that in vivo Miglustat administration in NPC1-/- mice was able to rescue synaptic plasticity deficits, to restore ERKs activation and to counteract hyperexcitability. Overall, these data indicate that Miglustat may be effective for treating the neurological deficits associated with NPCD, such as seizures and dementia

TAp73 promotes anabolism

Metabolic adaptation has emerged as a hallmark of cancer and a promising therapeutic target, as rapidly proliferating cancer cells adapt their metabolism increasing nutrient uptake and reorganizing metabolic fluxes to support biosynthesis. The transcription factor p73 belongs to the p53-family and regulates tumorigenesis via its two N-terminal isoforms, with (TAp73) or without (ΔNp73) a transactivation domain. TAp73 acts as tumor suppressor, at least partially through induction of cell cycle arrest and apoptosis and through regulation of genomic stability. Here, we sought to investigate whether TAp73 also affects metabolic profiling of cancer cells. Using high throughput metabolomics, we unveil a thorough and unexpected role for TAp73 in promoting Warburg effect and cellular metabolism. TAp73-expressing cells show increased rate of glycolysis, higher amino acid uptake and increased levels and biosynthesis of acetyl-CoA. Moreover, we report an extensive TAp73-mediated upregulation of several anabolic pathways including polyamine and synthesis of membrane phospholipids. TAp73 expression also increases cellular methyl-donor S-adenosylmethionine (SAM), possibly influencing methylation and epigenetics, and promotes arginine metabolism, suggestive of a role in extracellular matrix (ECM) modeling. In summary, our data indicate that TAp73 regulates multiple metabolic pathways that impinge on numerous cellular functions, but that, overall, converge to sustain cell growth and proliferation

Caracterização biométrica e físico-química dos frutos de acessos de manga 'Ubá'.

Este trabalho teve como objetivo avaliar a dissimilaridade genética de acessos de manga ?Ubá? na região leste de Minas Gerais, por meio da caracterização biométrica e físico-química dos frutos, visando a identificar materiais de interesse industrial para futuros trabalhos de melhoramento. Frutos de 67 acessos de mangueira Ubá provenientes do leste de Minas Gerais foram caracterizados, avaliando-se: massa da fruta, massa do endocarpo, relação polpa/endocarpo, diâmetro longitudinal, diâmetro transversal, sólidos solúveis totais, acidez total titulável, relação sólidos solúveis total/acidez total titulável e pH. Os resultados foram avaliados por estatística descritiva, utilizando-se de medida de tendência central (média) e de variabilidade de dados (desvio-padrão). Foram realizadas análises estatísticas, utilizando-se das técnicas de agrupamento e medidas de dissimilaridade. Os frutos que apresentaram melhores características para o processamento foram os provenientes dos acessos 10; 22; 23; 24; 35; 38; 40; 42; 52; 55 e 63. A análise de agrupamento mostrou a formação de dois grupos de acessos com base nas características biométricas e físico-químicas dos frutos, o que demonstra variabilidade genética em mangueiras Ubá, no leste de Minas Gerais

DRY BEAN CULTIVAR IAC ALVORADA UNDER DIFFERENT RATES OF \u3ci\u3eRHIZOBIUM\u3c/i\u3e INOCULANT IN THE PLANTING FURROW

INTRODUCTION: No publications were found regarding liquid inoculation in the planting furrow of dry bean, which indicates a demand for investigations to assist the producer in deciding which method of inoculation to adopt. Thus, the aim of this study was to evaluate the viability of liquid inoculation in the planting furrow and determine the best application rate of inoculant, using the dry bean cultivar IAC Alvorada. MATERIALS AND METHODS: A field experiment was conducted in a no-till planting system in the 2014/2015 crop season in a Latossolo Vermelho distrófico in the municipality of Lambari, MG, Brazil. The experimental design was randomized blocks with three replications and eight treatments, involving five application rates of liquid inoculant in the planting furrow (0, 120, 240, 480, and 720 mL ha-1), liquid seed inoculation (40 mL kg-1), and two controls without inoculation: one with N-urea (80 kg ha-1 N, ½ at sowing and ½ in topdressing, between the V3 and V4 stages of the crop cycle) and another without mineral N. The experimental unit (14.4 m2) consisted of six four-meter-length rows, spaced at 0.6 m, and the area effectively used was the four central rows. All the plots received base fertilization of 110 kg ha-1 of P2O5 (simple superphosphate) and 40 kg ha-1 of K2O (potassium chloride), mechanically applied during furrowing. In addition, all levels of inoculation received 20 kg Nurea ha-1, so as to meet the recommendations of Soares et al. (2016). Sowing was manual at the density of 15 seeds per meter and the cultivar used was IAC Alvorada, of semi-upright plant architecture and moderate resistance to anthracnose (Carbonell et al., 2008)

DRY BEAN CULTIVAR IAC ALVORADA UNDER DIFFERENT RATES OF \u3ci\u3eRHIZOBIUM\u3c/i\u3e INOCULANT IN THE PLANTING FURROW

INTRODUCTION: No publications were found regarding liquid inoculation in the planting furrow of dry bean, which indicates a demand for investigations to assist the producer in deciding which method of inoculation to adopt. Thus, the aim of this study was to evaluate the viability of liquid inoculation in the planting furrow and determine the best application rate of inoculant, using the dry bean cultivar IAC Alvorada. MATERIALS AND METHODS: A field experiment was conducted in a no-till planting system in the 2014/2015 crop season in a Latossolo Vermelho distrófico in the municipality of Lambari, MG, Brazil. The experimental design was randomized blocks with three replications and eight treatments, involving five application rates of liquid inoculant in the planting furrow (0, 120, 240, 480, and 720 mL ha-1), liquid seed inoculation (40 mL kg-1), and two controls without inoculation: one with N-urea (80 kg ha-1 N, ½ at sowing and ½ in topdressing, between the V3 and V4 stages of the crop cycle) and another without mineral N. The experimental unit (14.4 m2) consisted of six four-meter-length rows, spaced at 0.6 m, and the area effectively used was the four central rows. All the plots received base fertilization of 110 kg ha-1 of P2O5 (simple superphosphate) and 40 kg ha-1 of K2O (potassium chloride), mechanically applied during furrowing. In addition, all levels of inoculation received 20 kg Nurea ha-1, so as to meet the recommendations of Soares et al. (2016). Sowing was manual at the density of 15 seeds per meter and the cultivar used was IAC Alvorada, of semi-upright plant architecture and moderate resistance to anthracnose (Carbonell et al., 2008)

P73 in cancer

p73 is a tumor suppressor belonging to the p53 family of transcription factors. Distinct isoforms are transcribed from the p73 locus. The use of 2 promoters at the N-terminus allows the expression of an isoform containing (TAp73) or not containing (ΔNp73) a complete N-terminal transactivation domain, with the latter isoform capable of a dominant negative effect over the former. In addition, both N-terminal variants are alternatively spliced at the C-terminus. TAp73 is a bona fide tumor suppressor, being able to induce cell death and cell cycle arrest; conversely, ΔNp73 shows oncogenic properties, inhibiting TAp73 and p53 functions. Here, we discuss the latest findings linking p73 to cancer. The generation of isoform specific null mice has helped in dissecting the contribution of TA versus ΔNp73 isoforms to tumorigenesis. The activity of both isoforms is regulated transcriptionally and by posttranslational modification. p73 dysfunction, particularly of TAp73, has been associated with mitotic abnormalities, which may lead to polyploidy and aneuploidy and thus contribute to tumorigenesis. Although p73 is only rarely mutated in cancer, the tumor suppressor actions of TAp73 are inhibited by mutant p53, a finding that has important implications for cancer therapy. Finally, we discuss the expression and role of p73 isoforms in human cancer, with a particular emphasis on the neuroblastoma cancer model. Broadly, the data support the hypothesis that the ratio between TAp73 and ΔNp73 is crucial for tumor progression and therapeutic response