21 research outputs found
Conjugated linoleic acid, unlike other unsaturated fatty acids, strongly induces glutathione synthesis without any lipoperoxidation
International audienc
IMMUNOMODULATION DU MICRO-ENVIRONNEMENT TUMORAL MAMMAIRE : L'EXERCICE PHYSIQUE CONTREBALANCE L'EFFET TOLĂROGĂNE DE LA VITAMINE D
International audienc
CaractĂ©risation de lâinfiltrat immunitaire tumoral mammaire chez la souris C57BL/6 obĂšse
National audienceLa rĂ©ponse immunitaire systĂ©mique et tissulaire joue un rĂŽle-clĂ© dans la prĂ©vention du dĂ©veloppement tumoral. Notre objectif est dâĂ©valuer les modifications de lâinfiltrat immunitaire tumoral dans un modĂšle murin syngĂ©nique orthotopique de carcinogenĂšse mammaire en situation dâobĂ©sitĂ©. Des souris femelles C57BL/6 ĂągĂ©es (33 semaines) ovariectomisĂ©es, nourries avec un rĂ©gime Hyperlipidique (HL) ou Standard (SD), ont reçu une injection de cellules tumorales (EO771) par la technique « fatpad ». Au sacrifice, un phĂ©notypage des cellules immunitaires intra-tumorales a Ă©tĂ© rĂ©alisĂ© par cytomĂ©trie en flux. Le phĂ©notypage des cellules immunitaires intra-tumorales rĂ©vĂšle, sous rĂ©gime HL vs SD, une augmentation des populations immunosuppressives (MDSC : 278 ± 42 vs 57 ± 17 cellules (C) /mg tumeur (T), p=0,02 ; LTreg : 1,2 ± 0.3 vs 11,23 ± 17,4 C /mg T, p=0,013 ), associĂ©e Ă une modification de la rĂ©partition des sous-types de lymphocytes T (LTc / LTreg : 1,4 ± 0,1 vs 12 ± 6,8, p=0,05). De plus une tendance Ă lâaugmentation des populations anti-tumorales est observĂ©e (NKT : 364 ± 70 vs 491 ± 113 C /mg T, p=0,13 ; NK : 7,4 ±1,41 vs 43 ± 16 C /mg T, p=0,013). Ainsi, le rĂ©gime HL favorise la migration des cellules immunitaires depuis les organes lymphoĂŻdes secondaires vers la tumeur, induisant une repolarisation de lâinfiltrat immunitaire tumoral en faveur des cellules immunosuppressives impliquĂ©es dans le processus de carcinogenĂšse en dĂ©pit dâune immunosurveillance renforcĂ©
Effects of Enriched Environment on COX-2, Leptin and Eicosanoids in a Mouse Model of Breast Cancer
Cyclooxygenase-2 (COX-2) and adipokines have been implicated in breast cancer. This study investigated a possible link between COX-2 and adipokines in the development of mammary tumors. A model of environmental enrichment (EE), known to reduce tumor growth was used for a syngeneic murine model of mammary carcinoma. 3-week-old, female C57BL/6 mice were housed in standard environment (SE) or EE cages for 9 weeks and transplanted orthotopically with syngeneic EO771 adenocarcinoma cells into the right inguinal mammary fat pad. EE housing influenced mammary gland development with a decrease in COX-2 expressing cells and enhanced side-branching and advanced development of alveolar structures of the mammary gland. Tumor volume and weight were decreased in EE housed mice and were associated with a reduction in COX-2 and Ki67 levels, and an increase in caspase-3 levels. In tumors of SE mice, high COX-2 expression correlated with enhanced leptin detection. Non-tumor-bearing EE mice showed a significant increase in adiponectin levels but no change in those of leptin, F2-isoprostanes, PGF2α, IL-6, TNF-α, PAI-1, and MCP-1 levels. Both tumor-bearing groups (SE and EE housing) had increased resistin, IL-6, TNF-α, PAI-1 and MCP-1 levels irrespective of the different housing environment demonstrating higher inflammatory response due to the presence of the tumor. This study demonstrates that EE housing influenced normal mammary gland development and inhibited mammary tumor growth resulting in a marked decrease in intratumoral COX-2 activity and an increase in the plasma ratio of adiponectin/leptin levels
Cell Cycle Synchronization of the Murine EO771 Cell Line Using Double Thymidine Block Treatment
International audienceThis study shows that double thymidine block treatment efficiently arrests the EO771 cells in the S-phase without altering cell growth or survival. A long-term analysis of cell behavior, using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE) staining, show synchronization to be stable and consistent over time. The EO771 cell line is a medullary breast-adenocarcinoma cell line isolated from a spontaneous murine mammary tumor, and can be used to generate murine tumor implantation models. Different biological (serum or amino acid deprivation), physical (elutriation, mitotic shake-off), or chemical (colchicine, nocodazole, thymidine) treatments are widely used for cell synchronization. Of the different methods tested, the double thymidine block is the most efficient for synchronization of murine EO771 cells if a large quantity of highly synchronized cells is recommended to study functional and biochemical events occurring in specific points of cell cycle progression
Increased consumption of salmon during pregnancy partly prevents the decline of some plasma essential amino acid concentrations in pregnant women
International audienceBackground & aims: Oily fish is a good source of n-3 long-chain polyunsaturated fatty acids. Since these fatty acids may change efficiency of amino acid (AA) absorption, we determined whether increased salmon consumption influences plasma AA concentrations in pregnant women and their newborns. Methods: Pregnant women were randomly allocated to remain on their habitual diet (n = 61; control group) or to consume two 150 g farmed salmon portions per week from 20 weeks pregnancy until birth (n = 62; salmon group). Plasma AA concentrations were determined in women at w20, w34 and w38 of pregnancy and in umbilical cord at delivery. Results: Concentrations of arginine, valine, leucine and lysine were affected by both time of pregnancy and salmon intake (p 0.05). Conclusions: Two portions/wk of oily fish increased plasma essential AA concentrations during pregnancy and could contribute to a maternal health benefit. Two portions/wk of salmon did not affect plasma AA concentrations in the newborn. (C) 2013 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved
Association entre profils métabolomiques plasmatiques par rmn et risque à long terme de développer un cancer de la prostate
National audienc
Mise en place de l'enrichissement : impact des phases du protocole sur la composition corporelle et la croissance tumorale dans un modÚle murin de carcinogenÚse mammaire associé à un régime hyper-lipidique
National audienceAlors que l'arrĂȘtĂ© de fĂ©vrier 2013 1 encourage l'enrichissement du milieu des animaux en expĂ©rimentation sans donner plus de prĂ©cision, nous verrons que cette pratique n'est pas sans consĂ©quence sur les Ă©tudes en oncologie. En effet, l'activitĂ© physique est reconnue comme un facteur protecteur pour de nombreux cancers 2. Cependant, les mĂ©canismes d'interaction entre l'activitĂ© physique et le dĂ©veloppement de la tumeur sont mal compris. Depuis plusieurs annĂ©es, notre laboratoire travaille sur cette problĂ©matique grĂące Ă un modĂšle syngĂ©nique de souris C57BL6 implantĂ©es par fat-pad avec des cellules EO771 de carcinome mammaire 3,4,5. Nous discuterons de l'impact des diffĂ©rentes Ă©tapes du protocole expĂ©rimental (ovariectomie, rĂ©gime alimentaire, implantation tumorale) et de l'introduction de l'enrichissement du milieu sur les rĂ©sultats des Ă©tudes dans le champ de la carcinogenĂšse mammaire associĂ© Ă un rĂ©gime hyper-lipidique