Process disturbances in agricultural biogas production—causes, mechanisms and effects on the biogas microbiome: A review

Disturbances of the anaerobic digestion process reduce the economic and environmental performance of biogas systems. A better understanding of the highly complex process is of crucial importance in order to avoid disturbances. This review defines process disturbances as significant changes in the functionality within the microbial community leading to unacceptable and severe decreases in biogas production and requiring an active counteraction to be overcome. The main types of process disturbances in agricultural biogas production are classified as unfavorable process temperatures, fluctuations in the availability of macro- and micronutrients (feedstock variability), overload of the microbial degradation potential, process-related accumulation of inhibiting metabolites such as hydrogen (H 2 ), ammonium/ammonia (NH 4 + /NH 3 ) or hydrogen sulphide (H 2 S) and inhibition by other organic and inorganic toxicants. Causes, mechanisms and effects on the biogas microbiome are discussed. The need for a knowledge-based microbiome management to ensure a stable and efficient production of biogas with low susceptibility to disturbances is derived and an outlook on potential future process monitoring and control by means of microbial indicators is provided

Circulação de cromo entre os compartimentos biótico e abiótico com indícios de bioindicação em uma turfeira não alterada no município de Guaíba, RS, Brasil

Amostras de sedimento, água e tecido vegetal (Mimosa bimucronata e Paspalum notatum)foram analisadas com respeito a seu conteúdo em cromo. Estas amostras foram coletadas de umaturfeira numa baixada entre elevações graníticas na Fazenda São Maximiniano, município de Guaíba,RS, Brasil. Os resultados indicam que em um meio sem contaminação antrópica do metal propostoas concentrações do metal pesado estão totalmente dependentes da fonte para este, ou seja, arocha granítica. O presente trabalho permitiu estabelecer os níveis naturais de cromo (background)em um ambiente geologicamente conhecido e não alterado antropicamente, assim como o comportamentogeoquímico deste metal entre os compartimentos abióticos e biótico. Foi possívelestabelecer, também, o comportamento de absorção do metal por duas espécies vegetais tidascomo bioindicadoras.Samples of sediment, water and vegetational tissue (Mimosa bimucronata and Paspalumnotatum) were analised with respect of its chromium content. These samples were collected from apeatland located in a basin among granitic elevations in the São Maximiniano Farm, Guaíba municipality,Rio Grande do Sul, Brazil. The results indicate that in a non antropic contaminated environment by theproposed heavy metal, the concentration are totally source dependent for it, the granitic rocks. Thiswork led us to establish the natural levels of chromium (background) in a geologically well known andnon antropically changed environment, as well as geochemical behavior of this metal among theabiotics and biotic compartments. It was also possible to establish the biosorption behavior of the twovegetal species, known as bioindicators

Shear bands in granular flow through a mixing length model

We discuss the advantages and results of using a mixing-length, compressible model to account for shear banding behaviour in granular flow. We formulate a general approach based on two function of the solid fraction to be determined. Studying the vertical chute flow, we show that shear band thickness is always independent from flowrate in the quasistatic limit, for Coulomb wall boundary conditions. The effect of bin width is addressed using the functions developed by Pouliquen and coworkers, predicting a linear dependence of shear band thickness by channel width, while literature reports contrasting data. We also discuss the influence of wall roughness on shear bands. Through a Coulomb wall friction criterion we show that our model correctly predicts the effect of increasing wall roughness on the thickness of shear bands. Then a simple mixing-length approach to steady granular flows can be useful and representative of a number of original features of granular flow.Comment: submitted to EP

Water use indicators at farm scale: Methodology and case study

Indicators for water use at farm scale can assist farmers in understanding the water flows on their farms and in optimizing water use by adapting agronomic measures and farm management. The objective of this work is to develop a methodology to estimate water flows at the farm scale, to derive indicators for farm water use, and to apply them in a first case study. After the spatial and temporal boundaries of the farm system and the water flows are defined, three indicators to assess water use at the farm scale are developed: farm water productivity, degree of water utilization, and specific inflow of technical water. Farm water productivity describes the ratio of farm output to water input, where the water input is the total of those water inflows into the farm system that can be assigned to the generation of farm output. Farm output is expressed on a mass basis, food energy basis, and monetary basis. The degree of water utilization characterizes the relationship between productive water to the total water inflow into the farm system, where productive water comprises those water flows that directly contribute to biomass generation via plant and animal metabolism. The specific technical water inflow quantifies the water inflow into the system by technical means relative to the farm area. The application of the methodology in a first case study for a mixed crop-livestock farm with 2869 ha in Germany results in a farm water productivity of 2.30 kg fresh mass per mWinput-3, 1.03 kg dry mass per m Winput-3, 5.96 GJ m Winput-3, and 0.25 € mWinput-3. The degree of water utilization is 0.56. The specific technical water inflow is 36.5 m3 ha-1 year -1. Factors that mainly effect these indicators and general approaches to optimize water use in farms are discussed as well as the further research required for practical implementation

Eficiência agronômica de fosfato natural reativo na cultura da soja.

O objetivo deste trabalho foi avaliar a eficiência agronômica relativa de uma fonte de fósforo na cultura de soja [Glycine max (L.) Merrill.]. O superfosfato triplo foi a fonte-padrão, e o fosfato natural reativo Arad foi a fonte testada, ambos aplicados em diferentes doses, em área total ou no sulco de semeadura. Em outubro de 2004, o experimento foi instalado em Balsas, MA, em Latossolo Vermelho-Amarelo distrófico, textura argilosa e baixa disponibilidade de fósforo, conduzido com soja cultivar BRS Sambaíba por três safras (2004/2005 a 2006/2007); a terceira safra foi conduzida sob efeito residual das aplicações anteriores. De forma geral, não foram observadas diferenças quanto à localização da fonte-padrão, ao passo que a localização do fosfato natural reativo Arad reduziu significativamente a eficiência. Quando aplicado a lanço, nos dois primeiros cultivos, o fosfato natural reativo Arad resultou em aproximadamente 76% de eficiência agronômica relativa, o que demonstra média viabilidade agronômica. Sob efeito residual, a aplicação localizada do superfosfato triplo resultou em resposta semelhante à verificada com a aplicação anual desta fonte, entretanto, o aumento na eficiência agronômica relativa foi mais acentuado, quando ambas as fontes foram aplicadas a lanço

Improving patient and caregiver new medication education using an innovative teach-back toolkit

Background: Patients and caregivers are often not adequately informed about new medications. Nurses can lead innovations that improve new medication education. Local Problem: Healthcare Consumer Assessment of Healthcare Providers and Systems (HCAHPS) scores on medication questions trailed state and national levels in one Midwestern hospital. Methods: This quality improvement project, guided by the Ottawa Model of Research Use and the Always Use Teach-back! innovative toolkit, used a 1-group pre- and post education design with RNs, patients, and caregivers. Intervention: RNs (n = 25) were observed in patient/caregiver education and surveyed in confidence/conviction in the teach-back method before and after education. Patients’ (n = 74) and caregivers’ (n = 33) knowledge was assessed. Results: RNs reported significant increases in conviction in the importance of (P \u3c .0001), confidence in using (P \u3c .0001), and frequency in using (P \u3c .0001) teach-back. With teach-back, both patients and caregivers recalled the purpose and side effects of new medications. Specific HCAHPS scores increased from 6% to 10%. Conclusion: The teach-back method strengthened safe nursing practice and enhanced quality in new medication education

Water footprint analysis for the assessment of milk production in Brandenburg (Germany)

The working group "Adaptation to Climate Change" at the Leibniz-Institute for Agricultural Engineering Potsdam-Bornim (ATB) is introduced. This group calculates the water footprint for agricultural processes and farms, distinguished into green water footprint, blue water footprint, and dilution water footprint. The green and blue water demand of a dairy farm plays a pivotal role in the regional water balance. Considering already existing and forthcoming climate change effects there is a need to determine the water cycle in the field and in housing for process chain optimisation for the adaptation to an expected increasing water scarcity. Resulting investments to boost water productivity and to improve water use efficiency in milk production are two pathways to adapt to climate change effects. In this paper the calculation of blue water demand for dairy farming in Brandenburg (Germany) is presented. The water used for feeding, milk processing, and servicing of cows over the time period of ten years was assessed in our study. The preliminary results of the calculation of the direct blue water footprint shows a decreasing water demand in the dairy production from the year 1999 with 5.98×109 L/yr to a water demand of 5.00×109 L/yr in the year 2008 in Brandenburg because of decreasing animal numbers and an improved average milk yield per cow. Improved feeding practices and shifted breeding to greater-volume producing Holstein-Friesian cow allow the production of milk in a more water sustainable way. The mean blue water consumption for the production of 1 kg milk in the time period between 1999 to 2008 was 3.94±0.29 L. The main part of the consumed water seems to stem from indirect used green water for the production of feed for the cows

Environmental cues and genes involved in establishment of the superinfective Pf4 phage of Pseudomonas aeruginosa

© 2014 Hui, Mai-Prochnow, Kjelleberg, McDougald and Rice. Biofilm development in Pseudomonas aeruginosa is in part dependent on a filamentous phage, Pf4, which contributes to biofilm maturation, cell death, dispersal and variant formation, e.g., small colony variants (SCVs). These biofilm phenotypes correlate with the conversion of the Pf4 phage into a superinfection (SI) variant that reinfects and kills the prophage carrying host, in contrast to other filamentous phage that normally replicate without killing their host. Here we have investigated the physiological cues and genes that may be responsible for this conversion. Flow through biofilms typically developed SI phage approximately days 4 or 5 of development and corresponded with dispersal. Starvation for carbon or nitrogen did not lead to the development of SI phage. In contrast, exposure of the biofilm to nitric oxide, H2O2 or the DNA damaging agent, mitomycin C, showed a trend of increased numbers of SI phage, suggesting that reactive oxygen or nitrogen species (RONS) played a role in the formation of SI phage. In support of this, mutation of oxyR, the major oxidative stress regulator in P. aeruginosa, resulted in higher level of and earlier superinfection compared to the wild-type (WT). Similarly, inactivation of mutS, a DNA mismatch repair gene, resulted in the early appearance of the SI phage and this was four log higher than the WT. In contrast, loss of recA, which is important for DNA repair and the SOS response, also resulted in a delayed and decreased production of SI phage. Treatments or mutations that increased superinfection also correlated with an increase in the production of morphotypic variants. The results suggest that the accumulation of RONS by the biofilm may result in DNA lesions in the Pf4 phage, leading to the formation of SI phage, which subsequently selects for morphotypic variants, such as SCVs

Phosphorus placement for annual crops in the tropics.

This article discusses principles for optimizing the placement of P in soils of the tropics?looking towards better agronomic, economic, environmental, and social outcomes. General guidelines are offered for short and long-term sustainability