Micropropagation of Origanum sipyleum L., an endemic medicinal herb of Turkey

Origanum sipyleum L. (Lamiaceae) is an endemic species of the Western Anatolia, Turkey. Essential oils of oreganos are utilized in pharmaceutical and cosmetic industries. A micropropagation protocol was developed using seedlings-derived explants. Shoot apices of 17d-old seedling were cultured and shoots multiplied on Murashige-Skoog modified (MSM) containing 550 mg/l of CaCl2 for sustained growth. Multiple shoots (3.7 ± 0.3 shoot/explant) were produced on medium containing 1 mg/L benzylaminopurine (BAP). On subculturing rated of shoot multiplication increased to 7.8 ± 0.4. 96% ofthe shoots rooted in a culture medium with 0.5 mg/L indolebutyric acid (IBA) after 3 weeks. The plantlets were acclimatized into outdoor conditions. 76% of these survived in the greenhouse. These in vitro derived microplants are already under the evaluation for their essential oil composition

High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hypericum triquetrifolium Turra

A method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semisolid MS supplemented with IAA (0.5 mg.L-1) combined with BAP (2 mg.L-1). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these embryogenic calli occured in semi-solid medium when the PGRs were removed completely. Embryogenic calli obtained during the experiments were analyzed for their hypericin content. And they were found to produce hypericin as 48 ìg/g DM. Regenerated plantlets were rooted in MS containing 1 mg/L IAA. The highest percentages (94%) of survival of transferred plantlets to freeliving circumstances were limited when they were acclimatized in sand : peat : perlite (1:1:1; v/v/v) mixture

High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperi{dotless}cum tri{dotless}quetri{dotless}foli{dotless}um Turra

A method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semi-solid MS supplemented with IAA (0.5 mg.L-1) combined with BAP (2 mg.L-1). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these embryogenic calli occured in semi-solid medium when the PGRs were removed completely. Embryogenic calli obtained during the experiments were analyzed for their hypericin content. And they were found to produce hypericin as 48 µg/g DM. Regenerated plantlets were rooted in MS containing 1 mg/L IAA. The highest percentages (94%) of survival of transferred plantlets to free-living circumstances were limited when they were acclimatized in sand: peat: perlite (1:1:1; v/v/v) mixture. © 2010 Academic Journals

Some biochemical characteristics of grafted watermelon

Grafting is an alternative approach to reduce crop damage resulting from soil-borne pathogens. This approach also increases plant abiotic stress tolerance, which in turn increases crop yield and quality. Therefore, the objective of this study was to determine the effect of grafting on watermelon quality parameters with the use of different rootstock combinations. Different morphological characteristics (yellow-red fleshed, with seed-seedless, striped rind and dark green rind) were grafted on rootstocks of Argentario (Lagenaria spp.) and Maximus (Cucurbita maxima × C. moschata). While the highest fructose, sucrose and soluble solids content were measured in Argentario/ TP84, TP84 had the highest glucose content. Total carotenoids content in watermelons ranged from 15.36 ± 2.78 to 104.51 ± 12.04 mg kg-1. Argentario/ TP84 showed the highest level of lycopene (93.95 mg kg-1) among cultivars and combinations. ß-carotene content varied from 1.44 ± 0.01 to 3.76 ± 0.01 mg kg-1. The highest ascorbic acid content was in Argentario/ TP84 (17.26 mg kg-1), followed by Argentario/ 5299 (17.26 mg kg-1). Grafting significantly increased soluble solids, sugars (fructose, glucose and sucrose), total carotenoids and ascorbic acid contents. Maximus/ Ant09 had the highest total carotenoids and lycopene content but lowest fructose, sucrose and soluble solids content on C. maxima × C. moschata combinations, whereas Argentario/ TP84 has the highest fructose, sucrose, soluble solids, total carotenoids, ß-carotene, lycopene and ascorbic acid contents among cultivars and combinations. This study showed that use of bottle gourd (Lagenaria spp.) rootstock could enhance soluble solids, fructose, sucrose, lycopene and ascorbic acid contents in watermelon. © 2017, Horticulture Society of India. All rights reserved

Investigation on fruit quality of grafted watermelon

10th EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae -- OCT 15-18, 2012 -- Antalya, TURKEYWOS: 000345687100045In this study, grafted and ungrafted watermelon cultivars were grown in Cukurova region to determine fruit pH, titratable acidity, soluble solids, yantioxidant activity, total phenolic compounds and analysis of sensory evaluation. The highest soluble solids (11.32%) and titratable acidity 0.190 g/100g were in seedless cultivars. The highest antioxidant activity and total phenolic content was in Argentario+TP84, 80.21% and 137.40 (GAE) mg/kg fresh weight (fw) respectively. For sensory analysis, the most popular cultivars were grafted type.European Assoc Res Plant Breeding, Cukurova Univ, Minis Food, Agr & Livestock, Turkish Sci & Technol Council, Antalya Tarim, Manier Seed, Yuksel Seed, Syngenta, AG Seed, Fito Seed, Multi Seed, Nunhems, Rijk Zwaan, Bati Akdeniz Agr Res Inst, Alata Hort Res Sta

Tetanus in adults: results of the multicenter ID-IRI study

Tetanus is an acute, severe infection caused by a neurotoxin secreting bacterium. Various prognostic factors affecting mortality in tetanus patients have been described in the literature. In this study, we aimed to analyze the factors affecting mortality in hospitalized tetanus patients in a large case series. This retrospective multicenter study pooled data of tetanus patients from 25 medical centers. The hospitals participating in this study were the collaborating centers of the Infectious Diseases International Research Initiative (ID-IRI). Only adult patients over the age of 15 years with tetanus were included. The diagnosis of tetanus was made by the clinicians at the participant centers. Izmir Bozyaka Education and Research Hospital’s Review Board approved the study. Prognostic factors were analyzed by using the multivariate regression analysis method. In this study, 117 adult patients with tetanus were included. Of these, 79 (67.5%) patients survived and 38 (32.5%) patients died. Most of the deaths were observed in patients >60 years of age (60.5%). Generalized type of tetanus, presence of pain at the wound area, presence of generalized spasms, leukocytosis, high alanine aminotransferase (ALT) and C-reactive protein (CRP) values on admission, and the use of equine immunoglobulins in the treatment were found to be statistically associated with mortality (p < 0.05 for all). Here, we describe the prognostic factors for mortality in tetanus. Immunization seems to be the most critical point, considering the advanced age of our patients. A combination of laboratory and clinical parameters indicates mortality. Moreover, human immunoglobulins should be preferred over equine sera to increase survival. © 2017, Springer-Verlag Berlin Heidelberg