In vitro assessment of cytotoxicity of giomer on human gingival fibroblasts

Root coverage on restored root surfaces has been considered as a challenging issue. The evaluation of cytotoxic effects of restorative materials is a fundamental requirement for sustaining the cell attachment and the clinical success of root coverage. The aim of the present study was to compare the human gingival fibroblast cytotoxicity of the recently introduced giomer composite (GC) with resin ionomer (RI) restorative material. Discs (6x2 mm) of GC and RI restorative materials were prepared using sterile Teflon mold. Extracts from the materials were incubated to cell culture medium for 24, 48 and 72 h. Human gingival fibroblasts (HGF) were exposed to the extracts of the materials while the un-incubated media served as the control group. The cytotoxicity of the materials were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In order to compare the mean values of the measured parameters a Kruskal-Walis test was carried out. MTT assay indicated that human gingival fibroblasts proliferated well in the presence of GC extract. The proliferation rate was higher in cells incubated with GC compared to RI extracts but the differences were not statistically significant (p= 0.09). This in vitro study indicated that GC is a non-toxic material for HGF. However, further studies are needed to assess the other biologic and clinical behavior of this material prior to it being considered as a potentially suitable restorative material to restore the carious root lesions candidated to root coverage procedures

Study of clonal variation of 'Bidaneh Ghermez' grapevine cultivar in Iran

ArticleGrapevine (Vitis vinifera L.) is a well - known plant including different cultivars and clones. In spite of the extensive works at the cultivar level, identification and determination of clonal genetic variation has remained as a challenge. To assess the genetic variation between clones of grapevine cv. 'Bidaneh Ghermez', 20 selected clones were analyzed for cluster weight (CW), cluster length (CL), cluster width (CWI), berry weight (BW), berry length (BL), berry width (BWI) and total soluble solids (TSS) in randomized complete block design with three replications. Analysis of variance reveale d considerable genetic variation for all measured traits (except cluster width) among clones. Cluster analysis, discriminant function analysis and principal component analysis (PCA) showed same results and all clones assigned in 2 groups. First group was including 9 clones and second group was including 11 clones. Overall, our results indicated C7, C10, C12 and C14 clones were best clones and have potential to introduce promising clones for stablishing new vineyard with high yield

In vitro assessment of cytotoxicity of giomer on human gingival fibroblasts

Root coverage on restored root surfaces has been considered as a challenging issue. The evaluation of cytotoxic effects of restorative materials is a fundamental requirement for sustaining the cellattachment and the clinical success of root coverage. The aim of the present study was to compare the human gingival fibroblast cytotoxicity of the recently introduced giomer composite (GC) with resinionomer (RI) restorative material. Discs (6×2 mm) of GC and RI restorative materials were prepared using sterile Teflon mold. Extracts from the materials were incubated to cell culture medium for 24, 48and 72 h. Human gingival fibroblasts (HGF) were exposed to the extracts of the materials while the unincubated media served as the control group. The cytotoxicity of the materials were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In order to compare the mean values of the measured parameters a Kruskal-Walis test was carried out. MTT assay indicated that human gingival fibroblasts proliferated well in the presence of GC extract. The proliferation rate washigher in cells incubated with GC compared to RI extracts but the differences were not statistically significant (p= 0.09). This in vitro study indicated that GC is a non-toxic material for HGF. However, further studies are needed to assess the other biologic and clinical behavior of this material prior to it being considered as a potentially suitable restorative material to restore the carious root lesions candidated to root coverage procedures

Identify successful restrictions in suppressing the early outbreak of COVID-19 in Arizona, United States: Interrupted time series analysis

COVID-19 was responsible for many deaths and economic losses around the globe since its first case report. Governments implemented a variety of policies to combat the pandemic in order to protect their citizens and save lives. Early in 2020, the first cases were reported in Arizona State and continued to rise until the discovery of the vaccine in 2021. A variety of strategies and interventions to stop or decelerate the spread of the pandemic has been considered. It is recommended to define which strategy was successful for disease propagation prevention and could be used in further similar situations. This study aimed to evaluate the effect of people's contact interventions strategies which were implemented in Arizona State and their effect on reducing the daily new COVID-19 cases and deaths. Their effect on daily COVID-19 cases and deaths were evaluated using an interrupted time series analysis during the pandemic's first peaks to better understand the onward situation. Canceling the order of staying at home (95% CI, 1718.52 to 6218.79; p<0.001) and expiring large gatherings (95% CI, 1984.99 to 7060.26; p<0.001) on June 30 and August 17, 2020, respectively, had a significant effect on the pandemic, leading to the daily cases to grow rapidly. Moreover, canceling the stay at home orders led to an increase in the number of COVID-19 daily deaths by 67.68 cases (95% CI, 27.96 to 107.40; p<0.001) after about 21 days while prohibiting large gatherings significantly decreased 66.76 (95% CI: 20.56 to 112.96; p = 0.004) the number of daily deaths with about 21 days' lag. The results showed that strategies aimed at reducing people's contact with one another could successfully help fight the pandemic. Findings from this study provide important evidence to support state-level policies that require observance of social distancing by the general public for future pandemics. Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

Background: Silkworm products were first used by physicians more than 8500 years ago, in the early Neolithic period. In Persian medicine, silkworm extract has several uses for treating and preventing neurological, cardiac, and liver diseases. Mature silkworms (Bombyx mori) and their pupae contain a variety of growth factors and proteins that can be used in many repair processes, including nerve regeneration. Objectives: The study aimed to evaluate the effects of mature silkworm (Bombyx mori), and silkworm pupae extract on Schwann cell proliferation and axon growth. Methods: Silkworm (Bombyx mori) and silkworm pupae extracts were prepared. Then, the concentration and type of amino acids and proteins in the extracts were evaluated by Bradford assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and liquid chromatograph-mass spectrometer (LC-MS/MS). Also, the regenerative potential of extracts for improving Schwann cell proliferation and axon growth was examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, electron microscopy, and NeuroFilament-200 (NF-200) immunostaining. Results: According to the results of the Bradford test, the total protein content of pupae extract was almost twice that of mature worm extract. Also, SDS-PAGE analysis revealed numerous proteins and growth factors, such as bombyrin and laminin, in extracts that are involved in the repair of the nervous system. In accordance with Bradford’s results, the evaluation of extracts using LCMS/MS revealed that the number of amino acids in pupae extract was higher than in mature silkworm extract. It was found that the proliferation of Schwann cells at a concentration of 0.25 mg/mL in both extracts was higher than the concentrations of 0.01 and 0.05 mg/mL. When using both extracts on dorsal root ganglion (DRGs), an increase in length and number was observed in axons. Conclusions: The findings of this study demonstrated that extracts obtained from silkworms, especially pupae, can play an effective role in Schwann cell proliferation and axonal growth, which can be strong evidence for nerve regeneration, and, consequently, repairing peripheral nerve damage

Enhancing Controlled Environment Agriculture in Desert Ecosystems with AC/DC Hybrid Solar Technology

Received: 29 March 2023. Accepted: 26 May 2023.Controlled Environment Agriculture (CEA) plays a crucial role in promoting sustainable farming practices within the challenging climate of the Arabian Peninsula. Traditional CEAs, however, are confronted with excessive water and electricity consumption due to the region's elevated temperatures and humidity levels. To address these challenges, an innovative project was carried out at the Al Dhaid Research Station, United Arab Emirates, integrating solar-powered cooling and irrigation, closed hydroponic systems, net-house structures, root zone cooling, and ultra-low-energy drippers. The study employed a cooled greenhouse alongside two net houses, one of which was equipped with a solar-powered cooling and irrigation system. Cucumber crops were cultivated within each structure, demonstrating that the combined technologies could prolong production periods despite increasing temperatures, while simultaneously reducing energy consumption by 95% and water usage by 80%, without compromising crop yield. The findings of this study suggest that the implementation of this novel approach holds significant potential for boosting crop productivity and water efficiency in desert agriculture systems.This publication showcases a collaborative effort between ICARDA and NARS in the countries of the Arabian Peninsula. The contributions and efforts of the NARS, particularly in the UAE where this study was conducted, are highly acknowledged and appreciated. We extend our heartfelt gratitude to the Arab Fund for Economic and Social Development (AFESD) and The Kuwait Fund for Arab Economic Development (KFAED) for their invaluable financial support to ICARDA-APRP. Their generous contributions have played a crucial role in facilitating the research and implementation of this project

Date Value Chain Analysis, Development, and Competitiveness of Date Palm Products in the Sultanate of Oman

This paper considers the vision of how to develop an efficient value chain and development strategies to improve the value chain in Oman. The main key factors of inclusive and sustainable development of the date value chain are determined to carry out the appropriate actions for improving the date palm sector in Oman. To explore the challenges and opportunities leading to the improvement of the marketing, commercialization, and competitiveness of dates and date palm products in this country, an analytical tool guideline (SWOT) was framed. Such analysis is useful to raise awareness to create policies for improved marketing of dates. Based on the SWOT analysis, the diversification of date varieties, and orientation toward modern plantations are the most important strengths of the date value chain. However, the increasing competition in regional and global date markets is the major threat to the date sector in Oman. There is a good possibility to promote the date processing industry in Oman given the rising demand for fresh and processed dates in national and international markets. A profitable and competitive date palm sector could be achieved by focusing on high yield and commercial varieties to ensure higher date palm productivity and the orientation toward adoption of quality standards to meet international market demand

Comparison of Cold-Resistance in Some Iranian and European Grape Cultivars

One of the main problems in vineyards of Iran is sensitivity of grape cultivars to winter cold. Considering the rich genetic diversity of grape cultivars in Iran vineyards and severe cold winter in 2007, this situation was used as a natural treatment in order to identify cold tolerance and assess damages in 21 Iranian grape varieties and 8 European grape varieties in a factorial experiment, based on completely randomized blocks design with four replications and three plants per unit. In each plant, four branches (one year old) were selected and the number of healthy and damaged sprouts were counted. Then, statistical and cluster analysis was performed. Descriptive statistics (coefficient of variation of phenotypic traits) of the obtained data from assessment of grape cultivars was calculated. Using the correlation coefficient (Pearson r), bilateral relationship between traits was measured and the attributes which their selection could increase the efficiency were determined. The results indicated that grape cultivars were located in four groups of sensitive, semi-sensitive, semi-tolerant and completely tolerant. Ghermez Bidaneh, Fakhri, Siyah Gazvin, Khalili and Rish Baba cultivars showed the highest tolerance. Most of the European cultivars including Ruby Seedless, Superior, Fiesta, Black Seedless, Perlette, Flame Seedless, Tompson Seedless and Torkmanistan 4 were in semi-tolerant to susceptible groups. Tolerance threshold against winter cold was was 15 ±1 ◦C in normal varieties and 20 ±1 ◦C in tolerant cultivars. There was a significant phenotypic correlation between the studied traits